################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 99 CGCG CG^CG AlwNI 181 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG BbeI 42 GGCGCC GGCGC^C BclI 353 TGATCA T^GATCA BmyI 533 GTGCCC G[AGT]GC[ACT]^C BpmI 214 CTCCAG CTGGAG(16/14) Bsp1286I 533 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 882 TGTACA T^GTACA BspDI 675 ATCGAT AT^CGAT BspMI 390 ACCTGC ACCTGC(4/8) BsrDI 302 GCAATG GCAATG(2/0) BsrGI 882 TGTACA T^GTACA BstUI 99 CGCG CG^CG BstXI 449 CCAGAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 294 GGATCT [GA]^GATC[CT] CfoI 43 GCGC GCG^C ClaI 675 ATCGAT AT^CGAT CpoI 124 CGGACCG CG^G[AT]CCG DraIII 132 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 672 GATATC GAT^ATC EcoT22I 957 ATGCAT ATGCA^T FbaI 353 TGATCA T^GATCA HaeII 42 GGCGCC [GA]GCGC^[CT] HhaI 43 GCGC GCG^C HinP1I 43 GCGC G^CGC HincII 937 GTCAAC GT[CT]^[GA]AC HindII 937 GTCAAC GT[CT]^[GA]AC KasI 42 GGCGCC G^GCGCC KspI 98 CCGCGG CCGC^GG MflI 294 GGATCT [GA]^GATC[CT] MseI 979 TTAA T^TAA MvnI 99 CGCG CG^CG NarI 42 GGCGCC GG^CGCC NsiI 957 ATGCAT ATGCA^T RsrII 124 CGGACCG CG^G[AT]CCG SacII 98 CCGCGG CCGC^GG ScaI 623 AGTACT AGT^ACT SfcI 588 CTATAG C^T[GA][CT]AG SfoI 42 GGCGCC GGC^GCC SmaI 200 CCCGGG CCC^GGG SpeI 487 ACTAGT A^CTAGT SspBI 882 TGTACA T^GTACA Tru9I 979 TTAA T^TAA TspRI 890 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 294 GGATCT [GA]^GATC[CT] XmaCI 200 CCCGGG C^CCGGG XmaI 200 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 200 CCCGGG C^[CT]CG[GA]G 283 CCCGAG BbsI 322 GAAGAC GAAGAC(2/6) 364 GAAGAC BpuAI 322 GAAGAC GAAGAC(2/6) 364 GAAGAC BsmBI 63 CGTCTC CGTCTC(1/5) 546 GAGACG BsmFI 82 GGGAC GGGAC(10/14) 460 GTCCC BsoBI 200 CCCGGG C^[CT]CG[GA]G 283 CCCGAG Cac8I 91 GCTGGC GC[ACGT]^[ACGT]GC 571 GCAAGC DsaI 98 CCGCGG C^C[GA][CT]GG 455 CCGTGG EcoT14I 158 CCAAGG C^C[AT][AT]GG 1010 CCAAGG HgaI 548 GACGC GACGC(5/10) 62 GCGTC MaeII 405 ACGT A^CGT 422 ACGT MspA1I 98 CCGCGG C[AC]G^C[GT]G 147 CCGCTG MwoI 91 GCTGGCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 901 GCAGATGAAGC NspI 347 ACATGC [GA]CATG^[CT] 1005 ACATGC PleI 32 GAGTC GAGTC(4/5) 515 GAGTC StyI 158 CCAAGG C^C[AT][AT]GG 1010 CCAAGG TaqI 506 TCGA T^CGA 676 TCGA TfiI 263 GAATC G^A[AT]TC 522 GAATC Tsp45I 344 GTGAC ^GT[GC]AC 629 GTGAC TthHB8I 506 TCGA T^CGA 676 TCGA XcmI 36 CCACCCGGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1023 CCATACCAACTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BanI BbvI BciVI BcnI BfaI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I