(Circular) (Six-base) MAPSORT of: pFN21AE3136.seq Check: 8613 from: 1 to: 5749 pFN21AE3136 5749 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 2404 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2801 3812 5196 5464 1632 Size: 1169 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1169 1011 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2191 3492 5064 2191 Size: 1301 1572 2876 Fragments arranged by size: 2876 1572 1301 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2288 3074 1870 Size: 73 262 83 786 4545 Fragments arranged by size: 4545 786 262 83 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2108 2171 3070 4525 Size: 325 217 627 260 61 63 899 1455 Cuts at: 4525 618 Size: 1842 Fragments arranged by size: 1842 1455 899 627 325 260 217 63 61 BanII G_rGCy'C Cuts at: 1391 3068 1391 Size: 1677 4072 BbeI G_GCGC'C Cuts at: 2051 2051 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2334 2376 928 Size: 971 435 42 4301 Fragments arranged by size: 4301 971 435 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2273 2308 2716 3641 4851 2273 Size: 35 408 925 1210 3171 Fragments arranged by size: 3171 1210 925 408 35 BclI T'GATC_A Cuts at: 1548 2358 1548 Size: 810 4939 BfrBI ATG'CAT Cuts at: 2964 2964 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2542 3810 4966 1790 Size: 752 1268 1156 2573 Fragments arranged by size: 2573 1268 1156 752 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 3480 4438 Size: 416 53 83 399 24 1201 862 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1201 958 862 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 4409 1239 Size: 965 2205 2579 Fragments arranged by size: 2579 2205 965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 2067 2554 3556 Size: 53 83 186 936 515 19 487 1002 Cuts at: 3556 3938 4573 275 Size: 382 635 1451 Fragments arranged by size: 1451 1002 936 635 515 487 382 186 83 53 19 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3033 3063 1178 Size: 30 165 30 1630 30 3864 Fragments arranged by size: 3864 1630 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2532 2679 2838 2854 1186 Size: 1346 147 159 16 4081 Fragments arranged by size: 4081 1346 159 147 16 BseYI C'CCAG_C Cuts at: 2467 4952 5448 2467 Size: 2485 496 2768 Fragments arranged by size: 2768 2485 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 2545 2074 Size: 471 5278 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2542 3068 3810 3895 4966 1391 Size: 399 752 526 742 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 526 399 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2404 3104 844 Size: 1560 700 3489 Fragments arranged by size: 3489 1560 700 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2314 4244 4418 65 Size: 1183 1066 1930 174 1396 Fragments arranged by size: 1930 1396 1183 1066 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 2887 96 Size: 2791 2958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2461 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2299 3079 3446 3831 3848 5300 5386 5398 Size: 327 780 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 780 385 367 346 327 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2103 2460 513 Size: 552 1038 357 3802 Fragments arranged by size: 3802 1038 552 357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 3265 4059 4079 3265 Size: 794 20 4935 Fragments arranged by size: 4935 794 20 ClaI AT'CG_AT Cuts at: 1554 2681 1554 Size: 1127 4622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DraIII CAC_nnn'GTG Cuts at: 2142 2142 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714 Size: 54 1425 50 275 18 1366 889 1629 Cuts at: 5714 8 Size: 43 Fragments arranged by size: 1629 1425 1366 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2801 3812 4409 5196 5464 1239 Size: 393 572 597 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 787 597 597 572 393 268 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 EcoRV GAT'ATC Cuts at: 2679 2679 Size: 5749 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2051 4896 1678 Size: 373 2845 2531 Fragments arranged by size: 2845 2531 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373 Size: 32 135 32 2818 32 42 32 2626 Fragments arranged by size: 2818 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2944 3093 3339 669 Size: 988 281 1006 149 246 3079 Fragments arranged by size: 3079 1006 988 281 246 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2047 2047 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2257 4863 5047 1305 Size: 952 2606 184 2007 Fragments arranged by size: 2606 2007 952 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3707 4066 4225 518 Size: 648 2541 359 159 2042 Fragments arranged by size: 2541 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2105 2154 3165 3842 4990 5235 1606 Size: 499 49 1011 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 677 499 245 49 NarI GG'CG_CC Cuts at: 2048 2048 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NsiI A_TGCA'T Cuts at: 2966 2966 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2356 3014 3107 4652 1132 Size: 97 1127 658 93 1545 2229 Fragments arranged by size: 2229 1545 1127 658 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 RsrII CG'GwC_CG Cuts at: 2130 2130 Size: 5749 SacII CC_GC'GG Cuts at: 2106 2106 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 2630 3997 5709 2630 Size: 1367 1712 2670 Fragments arranged by size: 2670 1712 1367 SfcI C'TryA_G Cuts at: 826 1045 2593 3097 4232 4913 5104 826 Size: 219 1548 504 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 681 504 219 191 SfoI GGC'GCC Cuts at: 2049 2049 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2207 3076 1872 Size: 335 869 4545 Fragments arranged by size: 4545 869 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 2492 152 Size: 2340 3409 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2163 3015 3188 513 Size: 552 1098 852 173 3074 Fragments arranged by size: 3074 1098 852 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 2189 3776 3793 3946 4131 4576 Size: 1255 567 42 1587 17 153 185 445 Cuts at: 4576 325 Size: 1498 Fragments arranged by size: 1587 1498 1255 567 445 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 2628 2887 Size: 274 80 33 51 1443 522 129 259 Cuts at: 2887 3995 5707 96 Size: 1108 1712 138 Fragments arranged by size: 1712 1443 1108 522 274 259 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2048 3035 2048 Size: 987 4762 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2205 3074 1870 Size: 335 869 4545 Fragments arranged by size: 4545 869 335 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI SrfI SwaI