(Circular) (Six-base) MAPSORT of: pFN21AE3307.seq Check: 8071 from: 1 to: 7099 pFN21AE3307 7099 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4909 278 Size: 53 83 186 936 3373 2468 Fragments arranged by size: 3373 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4442 1937 Size: 2505 4594 Acc65I G'GTAC_C Cuts at: 4420 4420 Size: 7099 AclI AA'CG_TT Cuts at: 1563 5226 5599 1563 Size: 3663 373 3063 Fragments arranged by size: 3663 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3151 4339 5162 6546 6814 1632 Size: 1519 1188 823 1384 268 1917 Fragments arranged by size: 1917 1519 1384 1188 823 268 AfeI AGC'GCT Cuts at: 2303 2303 Size: 7099 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6902 AflIII A'CryG_T Cuts at: 1203 5998 6975 1203 Size: 4795 977 1327 Fragments arranged by size: 4795 1327 977 AgeI A'CCGG_T Cuts at: 4139 5930 4139 Size: 1791 5308 AhdI GACnn_n'nnGTC Cuts at: 5828 5828 Size: 7099 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7099 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7099 AlwNI CAG_nnn'CTG Cuts at: 3118 4842 6414 3118 Size: 1724 1572 3803 Fragments arranged by size: 3803 1724 1572 ApaLI G'TGCA_C Cuts at: 3689 5156 6312 3689 Size: 1467 1156 4476 Fragments arranged by size: 4476 1467 1156 ApoI r'AATT_y Cuts at: 1493 3815 4408 4634 4786 6895 1493 Size: 2322 593 226 152 2109 1697 Fragments arranged by size: 2322 2109 1697 593 226 152 AseI AT'TA_AT Cuts at: 160 1032 5653 160 Size: 872 4621 1606 Fragments arranged by size: 4621 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2534 2608 4424 1870 Size: 73 77 514 74 1816 4545 Fragments arranged by size: 4545 1816 514 77 74 73 BamHI G'GATC_C Cuts at: 3245 3572 4429 4796 3245 Size: 327 857 367 5548 Fragments arranged by size: 5548 857 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2573 4420 5875 618 Size: 325 217 627 786 1847 1455 1842 Fragments arranged by size: 1847 1842 1455 786 627 325 217 BanII G_rGCy'C Cuts at: 1391 2320 2365 4418 1391 Size: 929 45 2053 4072 Fragments arranged by size: 4072 2053 929 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3855 928 Size: 971 1956 4172 Fragments arranged by size: 4172 1956 971 BbvCI CC'TCA_GC Cuts at: 2441 2453 2599 2441 Size: 12 146 6941 Fragments arranged by size: 6941 146 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5290 5324 1903 Size: 34 3353 34 3678 Fragments arranged by size: 3678 3353 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4991 6201 4991 Size: 1210 5889 BclI T'GATC_A Cuts at: 1548 3514 1548 Size: 1966 5133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5710 136 Size: 107 122 71 631 605 337 3701 1525 Fragments arranged by size: 3701 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7094 7094 Size: 7099 BlpI GC'TnA_GC Cuts at: 3335 4516 3335 Size: 1181 5918 Bme1580I G_kGCm'C Cuts at: 1790 2268 3693 5160 6316 1790 Size: 478 1425 1467 1156 2573 Fragments arranged by size: 2573 1467 1425 1156 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7099 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3967 4830 5788 Size: 416 53 83 399 24 2550 863 958 Cuts at: 5788 442 Size: 1753 Fragments arranged by size: 2550 1753 958 863 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7099 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2669 2669 Size: 7099 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3017 5759 1239 Size: 1778 2742 2579 Fragments arranged by size: 2742 2579 1778 Bpu10I CC'TnA_GC Cuts at: 2441 2453 2599 2441 Size: 12 146 6941 Fragments arranged by size: 6941 146 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5224 6089 6387 5224 Size: 865 298 5936 Fragments arranged by size: 5936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5762 882 Size: 617 4263 2219 Fragments arranged by size: 4263 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4890 493 Size: 711 3686 2702 Fragments arranged by size: 3686 2702 711 BsaBI GATnn'nnATC Cuts at: 3614 7093 3614 Size: 3479 3620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2347 4906 5288 Size: 53 83 186 936 624 190 2559 382 Cuts at: 5288 5923 275 Size: 635 1451 Fragments arranged by size: 2559 1451 936 635 624 382 190 186 83 53 BsaWI w'CCGG_w Cuts at: 2121 2931 3641 4139 5532 5930 6204 6351 6762 Size: 810 710 498 1393 398 274 147 411 Cuts at: 6762 2121 Size: 2458 Fragments arranged by size: 2458 1393 810 710 498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6874 Fragments arranged by size: 6874 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2697 2893 3044 3307 3310 3713 1186 Size: 1511 196 151 263 3 403 4572 Fragments arranged by size: 4572 1511 403 263 196 151 3 BseYI C'CCAG_C Cuts at: 2279 2420 2488 3088 4034 4200 6302 6798 2279 Size: 141 68 600 946 166 2102 496 2580 Fragments arranged by size: 2580 2102 946 600 496 166 141 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2401 1163 Size: 573 665 5861 Fragments arranged by size: 5861 665 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2084 2260 2437 2571 4549 5310 Size: 275 186 83 176 177 134 1978 761 Cuts at: 5310 6338 1540 Size: 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 177 176 134 83 BsiHKAI G_wGCw'C Cuts at: 2320 2365 3301 3693 3945 3972 4233 5160 5245 Size: 45 936 392 252 27 261 927 85 Cuts at: 5245 6316 2320 Size: 1071 3103 Fragments arranged by size: 3103 1071 936 927 392 261 252 85 45 27 BsmI GAATG_Cn' Cuts at: 3139 3407 3885 4610 4703 3139 Size: 268 478 725 93 5535 Fragments arranged by size: 5535 725 478 268 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2320 2365 3301 3693 3945 3972 Size: 399 478 52 45 936 392 252 27 Cuts at: 3972 4233 4418 5160 5245 6316 1391 Size: 261 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 936 742 478 399 392 261 252 185 85 52 45 27 BspEI T'CCGG_A Cuts at: 2931 6762 2931 Size: 3831 3268 BspHI T'CATG_A Cuts at: 3279 4176 4988 6718 3279 Size: 897 812 1730 3660 Fragments arranged by size: 3660 1730 897 812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4454 844 Size: 3610 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2401 2613 4986 1394 Size: 429 578 212 2373 3507 Fragments arranged by size: 3507 2373 578 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 3433 3540 5594 5768 65 Size: 1183 2185 107 2054 174 1396 Fragments arranged by size: 2185 2054 1396 1183 174 107 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 2274 3270 3771 4139 5743 5930 Size: 110 224 240 996 501 368 1604 187 Cuts at: 5930 1700 Size: 2869 Fragments arranged by size: 2869 1604 996 501 368 240 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7099 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7099 BssSI C'ACGA_G Cuts at: 5159 6171 5159 Size: 1012 6087 BstAPI GCAn_nnn'nTGC Cuts at: 794 3380 794 Size: 2586 4513 BstBI TT'CG_AA Cuts at: 5903 5903 Size: 7099 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7099 BstXI CCAn_nnnn'nTGG Cuts at: 2304 2304 Size: 7099 BstYI r'GATC_y Cuts at: 1972 3245 3572 3704 4429 4796 5181 5198 6650 Size: 1273 327 132 725 367 385 17 1452 Cuts at: 6650 6736 6748 7094 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1273 725 385 367 346 327 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2726 2766 2726 Size: 40 7059 BtgI C'CryG_G Cuts at: 513 1065 2004 4119 513 Size: 552 939 2115 3493 Fragments arranged by size: 3493 2115 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6360 Fragments arranged by size: 6360 683 56 BtsI GCAGTG_nn' Cuts at: 2108 3225 3921 3929 4615 5409 5429 2108 Size: 1117 696 8 686 794 20 3778 Fragments arranged by size: 3778 1117 794 696 686 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7064 DraI TTT'AAA Cuts at: 3073 3787 4403 3073 Size: 714 616 5769 Fragments arranged by size: 5769 714 616 DraIII CAC_nnn'GTG Cuts at: 2393 2393 Size: 7099 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2850 3199 6106 809 Size: 507 1534 349 2907 1802 Fragments arranged by size: 2907 1802 1534 507 349 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2081 2257 2434 Size: 54 1425 50 275 18 251 176 177 Cuts at: 2434 2464 4546 5435 7064 8 Size: 30 2082 889 1629 43 Fragments arranged by size: 2082 1629 1425 889 275 251 177 176 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2081 2257 2434 4546 1537 Size: 275 269 176 177 2112 4090 Fragments arranged by size: 4090 2112 275 269 177 176 EarI CTCTTCn'nnn_ Cuts at: 1353 3014 3806 4013 5029 1353 Size: 1661 792 207 1016 3423 Fragments arranged by size: 3423 1661 1016 792 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 2400 2569 5686 6060 6206 Size: 1064 138 668 143 169 3117 374 146 Cuts at: 6206 387 Size: 1280 Fragments arranged by size: 3117 1280 1064 668 374 169 146 143 138 EcoICRI GAG'CTC Cuts at: 2318 2363 2318 Size: 45 7054 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3017 3151 4339 5162 5759 6546 6814 Size: 393 1385 134 1188 823 597 787 268 Cuts at: 6814 1239 Size: 1524 Fragments arranged by size: 1524 1385 1188 823 787 597 393 268 134 EcoO109I rG'GnC_Cy Cuts at: 2024 2538 3115 3416 4129 2024 Size: 514 577 301 713 4994 Fragments arranged by size: 4994 713 577 514 301 EcoRI G'AATT_C Cuts at: 4408 4786 4408 Size: 378 6721 FspI TGC'GCA Cuts at: 5605 5605 Size: 7099 HaeII r_GCGC'y Cuts at: 1678 2305 6246 1678 Size: 627 3941 2531 Fragments arranged by size: 3941 2531 627 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 2926 2958 3013 Size: 32 135 32 1061 32 261 32 55 Cuts at: 3013 3045 5740 5772 5814 5846 1373 Size: 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 1061 261 135 55 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4443 4689 669 Size: 988 281 2505 246 3079 Fragments arranged by size: 3079 2505 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7099 HpaI GTT'AAC Cuts at: 4689 4689 Size: 7099 KpnI G_GTAC'C Cuts at: 4424 4424 Size: 7099 MfeI C'AATT_G Cuts at: 3507 4698 3507 Size: 1191 5908 MluI A'CGCG_T Cuts at: 6975 6975 Size: 7099 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2428 6213 6397 1305 Size: 903 220 3785 184 2007 Fragments arranged by size: 3785 2007 903 220 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5277 Fragments arranged by size: 5277 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 5057 5416 5575 518 Size: 648 1533 2358 359 159 2042 Fragments arranged by size: 2358 2042 1533 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2145 4038 4200 4515 5192 6340 6585 Size: 471 68 1893 162 315 677 1148 245 Cuts at: 6585 1606 Size: 2120 Fragments arranged by size: 2120 1893 1148 677 471 315 245 162 68 NaeI GCC'GGC Cuts at: 2036 2036 Size: 7099 NcoI C'CATG_G Cuts at: 513 1065 2004 4119 513 Size: 552 939 2115 3493 Fragments arranged by size: 3493 2115 939 552 NdeI CA'TA_TG Cuts at: 387 2050 387 Size: 1663 5436 NgoMIV G'CCGG_C Cuts at: 2034 2034 Size: 7099 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7099 NotI GC'GGCC_GC Cuts at: 4546 4546 Size: 7099 NspI r_CATG'y Cuts at: 1132 1229 4457 6002 1132 Size: 97 3228 1545 2229 Fragments arranged by size: 3228 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2472 1348 Size: 1124 5975 PciI A'CATG_T Cuts at: 5998 5998 Size: 7099 PflMI CCAn_nnn'nTGG Cuts at: 1108 3088 1108 Size: 1980 5119 PfoI T'CCnGG_A Cuts at: 4326 4326 Size: 7099 PmeI GTTT'AAAC Cuts at: 4403 4403 Size: 7099 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5168 5200 6681 6713 4343 Size: 32 793 32 1481 32 4729 Fragments arranged by size: 4729 1481 793 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2538 3115 3416 4129 2024 Size: 514 577 301 713 4994 Fragments arranged by size: 4994 713 577 514 301 PshAI GACnn'nnGTC Cuts at: 1613 2901 1613 Size: 1288 5811 PsiI TTA'TAA Cuts at: 4669 4669 Size: 7099 PstI C_TGCA'G Cuts at: 830 3215 4451 830 Size: 2385 1236 3478 Fragments arranged by size: 3478 2385 1236 PvuI CG_AT'CG Cuts at: 2001 2571 2001 Size: 570 6529 PvuII CAG'CTG Cuts at: 4038 4038 Size: 7099 SacI G_AGCT'C Cuts at: 2320 2365 2320 Size: 45 7054 SalI G'TCGA_C Cuts at: 1936 4441 1936 Size: 2505 4594 SanDI GG'GwC_CC Cuts at: 2538 2538 Size: 7099 SapI GCTCTTCn'nnn_ Cuts at: 3014 3806 4013 3014 Size: 792 207 6100 Fragments arranged by size: 6100 792 207 SbfI CC_TGCA'GG Cuts at: 4451 4451 Size: 7099 ScaI AGT'ACT Cuts at: 5347 7059 5347 Size: 1712 5387 SfcI C'TryA_G Cuts at: 826 1045 3211 4168 4447 5582 6263 6454 826 Size: 219 2166 957 279 1135 681 191 1471 Fragments arranged by size: 2166 1471 1135 957 681 279 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7099 SmaI CCC'GGG Cuts at: 1872 2022 2536 4426 1872 Size: 150 514 1890 4545 Fragments arranged by size: 4545 1890 514 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 5203 6104 6366 820 Size: 197 926 3260 901 262 1553 Fragments arranged by size: 3260 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4890 493 Size: 4397 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7099 SphI G_CATG'C Cuts at: 1132 4457 1132 Size: 3325 3774 SspI AAT'ATT Cuts at: 5 52 5023 5 Size: 47 4971 2081 Fragments arranged by size: 4971 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7099 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2549 2688 2961 2978 3867 Size: 552 939 264 281 139 273 17 889 Cuts at: 3867 4119 4538 513 Size: 252 419 3074 Fragments arranged by size: 3074 939 889 552 419 281 273 264 252 139 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2658 3125 5126 5143 5296 5481 5926 Size: 1255 1078 467 2001 17 153 185 445 Cuts at: 5926 325 Size: 1498 Fragments arranged by size: 2001 1498 1255 1078 467 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5345 7057 96 Size: 274 80 33 51 1443 3368 1712 138 Fragments arranged by size: 3368 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7059 7091 7059 Size: 32 7067 Tth111I GACn'n_nGTC Cuts at: 1316 2558 1316 Size: 1242 5857 XbaI T'CTAG_A Cuts at: 4435 4435 Size: 7099 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7099 XmaI C'CCGG_G Cuts at: 1870 2020 2534 4424 1870 Size: 150 514 1890 4545 Fragments arranged by size: 4545 1890 514 150 XmnI GAAnn'nnTTC Cuts at: 3097 3498 5228 3097 Size: 401 1730 4968 Fragments arranged by size: 4968 1730 401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4907 276 Size: 53 83 186 936 3373 2468 Fragments arranged by size: 3373 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI