(Circular) (Six-base) MAPSORT of: pFN21AE4208.seq Check: 5955 from: 1 to: 6889 pFN21AE4208 6889 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4699 278 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4232 1937 Size: 2295 4594 Acc65I G'GTAC_C Cuts at: 4210 4210 Size: 6889 AclI AA'CG_TT Cuts at: 1563 5016 5389 1563 Size: 3453 373 3063 Fragments arranged by size: 3453 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2602 4952 6336 6604 1632 Size: 970 2350 1384 268 1917 Fragments arranged by size: 2350 1917 1384 970 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6692 AflIII A'CryG_T Cuts at: 1203 2667 5788 6765 1203 Size: 1464 3121 977 1327 Fragments arranged by size: 3121 1464 1327 977 AgeI A'CCGG_T Cuts at: 5720 5720 Size: 6889 AhdI GACnn_n'nnGTC Cuts at: 5618 5618 Size: 6889 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2554 3275 1184 Size: 1370 721 4798 Fragments arranged by size: 4798 1370 721 AlwNI CAG_nnn'CTG Cuts at: 4632 6204 4632 Size: 1572 5317 ApaLI G'TGCA_C Cuts at: 4946 6102 4946 Size: 1156 5733 ApoI r'AATT_y Cuts at: 1493 2030 2556 2996 3929 4036 4134 4198 4424 Size: 537 526 440 933 107 98 64 226 Cuts at: 4424 4576 6685 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 933 537 526 440 226 152 107 98 64 AseI AT'TA_AT Cuts at: 160 1032 4118 5443 160 Size: 872 3086 1325 1606 Fragments arranged by size: 3086 1606 1325 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 AvaI C'yCGr_G Cuts at: 1870 1943 2326 3816 4214 1870 Size: 73 383 1490 398 4545 Fragments arranged by size: 4545 1490 398 383 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3433 3466 3433 Size: 33 6856 BamHI G'GATC_C Cuts at: 4219 4586 4219 Size: 367 6522 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3315 4210 5665 618 Size: 325 217 627 1528 895 1455 1842 Fragments arranged by size: 1842 1528 1455 895 627 325 217 BanII G_rGCy'C Cuts at: 1391 2097 4208 1391 Size: 706 2111 4072 Fragments arranged by size: 4072 2111 706 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3192 928 Size: 971 1293 4625 Fragments arranged by size: 4625 1293 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5080 5114 1903 Size: 34 3143 34 3678 Fragments arranged by size: 3678 3143 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4113 4781 5991 4113 Size: 668 1210 5011 Fragments arranged by size: 5011 1210 668 BclI T'GATC_A Cuts at: 1548 1548 Size: 6889 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5500 136 Size: 107 122 71 631 605 3828 1525 Fragments arranged by size: 3828 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6884 6884 Size: 6889 BlpI GC'TnA_GC Cuts at: 4306 4306 Size: 6889 Bme1580I G_kGCm'C Cuts at: 1790 2642 4950 6106 1790 Size: 852 2308 1156 2573 Fragments arranged by size: 2573 2308 1156 852 BmgBI CAC'GTC Cuts at: 1318 3259 1318 Size: 1941 4948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4620 5578 442 Size: 416 53 83 399 24 3203 958 1753 Fragments arranged by size: 3203 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6889 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3513 3519 5549 1239 Size: 2274 6 2030 2579 Fragments arranged by size: 2579 2274 2030 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2481 3045 5014 5879 6177 2481 Size: 564 1969 865 298 3193 Fragments arranged by size: 3193 1969 865 564 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3600 5552 882 Size: 617 2101 1952 2219 Fragments arranged by size: 2219 2101 1952 617 BsaAI yAC'GTr Cuts at: 493 1204 4680 493 Size: 711 3476 2702 Fragments arranged by size: 3476 2702 711 BsaBI GATnn'nnATC Cuts at: 2370 4050 6883 2370 Size: 1680 2833 2376 Fragments arranged by size: 2833 2376 1680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4696 5078 5713 275 Size: 53 83 186 936 3163 382 635 1451 Fragments arranged by size: 3163 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5322 5720 5994 6141 6552 5322 Size: 398 274 147 411 5659 Fragments arranged by size: 5659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 1178 Size: 30 165 30 838 30 5796 Fragments arranged by size: 5796 838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2962 3516 1186 Size: 1776 554 4559 Fragments arranged by size: 4559 1776 554 BseYI C'CCAG_C Cuts at: 2104 6092 6588 2104 Size: 3988 496 2405 Fragments arranged by size: 3988 2405 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3119 1163 Size: 573 1383 4933 Fragments arranged by size: 4933 1383 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4339 5100 6128 1540 Size: 275 186 2338 761 1028 2301 Fragments arranged by size: 2338 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 4950 5035 6106 2097 Size: 2853 85 1071 2880 Fragments arranged by size: 2880 2853 1071 85 BsmI GAATG_Cn' Cuts at: 2552 4400 4493 2552 Size: 1848 93 4948 Fragments arranged by size: 4948 1848 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2642 4208 4950 5035 6106 1391 Size: 399 307 545 1566 742 85 1071 2174 Fragments arranged by size: 2174 1566 1071 742 545 399 307 85 BspEI T'CCGG_A Cuts at: 6552 6552 Size: 6889 BspHI T'CATG_A Cuts at: 4778 6508 4778 Size: 1730 5159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3172 4244 844 Size: 2328 1072 3489 Fragments arranged by size: 3489 2328 1072 BsrBI CCG'CTC Cuts at: 1394 1823 4776 1394 Size: 429 2953 3507 Fragments arranged by size: 3507 2953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2548 5384 5558 65 Size: 1183 1300 2836 174 1396 Fragments arranged by size: 2836 1396 1300 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5533 5720 1700 Size: 110 3723 187 2869 Fragments arranged by size: 3723 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6889 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6889 BssSI C'ACGA_G Cuts at: 4949 5961 4949 Size: 1012 5877 BstAPI GCAn_nnn'nTGC Cuts at: 794 2547 794 Size: 1753 5136 BstBI TT'CG_AA Cuts at: 2404 5693 2404 Size: 3289 3600 BstEII G'GTnAC_C Cuts at: 1184 2068 1184 Size: 884 6005 BstYI r'GATC_y Cuts at: 1972 2371 4219 4586 4971 4988 6440 6526 6538 Size: 399 1848 367 385 17 1452 86 12 Cuts at: 6538 6884 1972 Size: 346 1977 Fragments arranged by size: 1977 1848 1452 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6150 Fragments arranged by size: 6150 683 56 BtsI GCAGTG_nn' Cuts at: 2761 4132 4405 5199 5219 2761 Size: 1371 273 794 20 4431 Fragments arranged by size: 4431 1371 794 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6854 DraI TTT'AAA Cuts at: 4193 4193 Size: 6889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3085 5896 809 Size: 507 1769 2811 1802 Fragments arranged by size: 2811 1802 1769 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4336 5225 6854 Size: 54 1425 50 275 18 2506 889 1629 Cuts at: 6854 8 Size: 43 Fragments arranged by size: 2506 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4336 1537 Size: 275 2524 4090 Fragments arranged by size: 4090 2524 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 2931 3009 3146 3596 4819 1353 Size: 1469 109 78 137 450 1223 3423 Fragments arranged by size: 3423 1469 1223 450 137 109 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5476 5850 5996 387 Size: 1064 138 3887 374 146 1280 Fragments arranged by size: 3887 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2095 2095 Size: 6889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2602 3513 3519 4952 5549 6336 6604 Size: 393 970 911 6 1433 597 787 268 Cuts at: 6604 1239 Size: 1524 Fragments arranged by size: 1524 1433 970 911 787 597 393 268 6 EcoRI G'AATT_C Cuts at: 4134 4198 4576 4134 Size: 64 378 6447 Fragments arranged by size: 6447 378 64 EcoRV GAT'ATC Cuts at: 2438 2438 Size: 6889 FspI TGC'GCA Cuts at: 5395 5395 Size: 6889 HaeII r_GCGC'y Cuts at: 1678 6036 1678 Size: 4358 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2241 2273 2930 Size: 32 135 32 632 32 5 32 657 Cuts at: 2930 2962 3371 3403 5530 5562 5604 5636 1373 Size: 32 409 32 2127 32 42 32 2626 Fragments arranged by size: 2626 2127 657 632 409 135 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 4233 4479 669 Size: 988 281 2295 246 3079 Fragments arranged by size: 3079 2295 988 281 246 HindIII A'AGCT_T Cuts at: 748 2780 3679 748 Size: 2032 899 3958 Fragments arranged by size: 3958 2032 899 HpaI GTT'AAC Cuts at: 4479 4479 Size: 6889 KpnI G_GTAC'C Cuts at: 4214 4214 Size: 6889 MfeI C'AATT_G Cuts at: 2964 4488 2964 Size: 1524 5365 MluI A'CGCG_T Cuts at: 6765 6765 Size: 6889 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2630 6003 6187 1305 Size: 684 641 3373 184 2007 Fragments arranged by size: 3373 2007 684 641 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5067 Fragments arranged by size: 5067 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 2595 3257 3662 3861 4847 5206 Size: 648 1003 426 662 405 199 986 359 Cuts at: 5206 5365 518 Size: 159 2042 Fragments arranged by size: 2042 1003 986 662 648 426 405 359 199 159 MspA1I CmG'CkG Cuts at: 1606 4305 4982 6130 6375 1606 Size: 2699 677 1148 245 2120 Fragments arranged by size: 2699 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6337 NdeI CA'TA_TG Cuts at: 387 387 Size: 6889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6889 NotI GC'GGCC_GC Cuts at: 4336 4336 Size: 6889 NspI r_CATG'y Cuts at: 1132 1229 2112 2671 3925 4247 5792 1132 Size: 97 883 559 1254 322 1545 2229 Fragments arranged by size: 2229 1545 1254 883 559 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6889 PciI A'CATG_T Cuts at: 2667 5788 2667 Size: 3121 3768 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6889 PmeI GTTT'AAAC Cuts at: 4193 4193 Size: 6889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4958 4990 6471 6503 4958 Size: 32 1481 32 5344 Fragments arranged by size: 5344 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6889 PsiI TTA'TAA Cuts at: 3294 4459 3294 Size: 1165 5724 PstI C_TGCA'G Cuts at: 830 3169 3652 4241 830 Size: 2339 483 589 3478 Fragments arranged by size: 3478 2339 589 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6889 SacI G_AGCT'C Cuts at: 2097 2097 Size: 6889 SalI G'TCGA_C Cuts at: 1936 4231 1936 Size: 2295 4594 SapI GCTCTTCn'nnn_ Cuts at: 3146 3146 Size: 6889 SbfI CC_TGCA'GG Cuts at: 4241 4241 Size: 6889 ScaI AGT'ACT Cuts at: 5137 6849 5137 Size: 1712 5177 SexAI A'CCwGG_T Cuts at: 3759 3759 Size: 6889 SfcI C'TryA_G Cuts at: 826 1045 2524 3165 3490 3648 3915 4237 5372 Size: 219 1479 641 325 158 267 322 1135 Cuts at: 5372 6053 6244 826 Size: 681 191 1471 Fragments arranged by size: 1479 1471 1135 681 641 325 322 267 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6889 SmaI CCC'GGG Cuts at: 1872 3818 4216 1872 Size: 1946 398 4545 Fragments arranged by size: 4545 1946 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 3060 4993 5894 6156 820 Size: 197 926 553 564 1933 901 262 1553 Fragments arranged by size: 1933 1553 926 901 564 553 262 197 SnaBI TAC'GTA Cuts at: 493 4680 493 Size: 4187 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6889 SphI G_CATG'C Cuts at: 1132 2112 3925 4247 1132 Size: 980 1813 322 3774 Fragments arranged by size: 3774 1813 980 322 SspI AAT'ATT Cuts at: 5 52 4813 5 Size: 47 4761 2081 Fragments arranged by size: 4761 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6889 StyI C'CwwG_G Cuts at: 513 1065 2567 4328 513 Size: 552 1502 1761 3074 Fragments arranged by size: 3074 1761 1502 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 2301 2705 4916 4933 5086 5271 Size: 1255 565 156 404 2211 17 153 185 Cuts at: 5271 5716 325 Size: 445 1498 Fragments arranged by size: 2211 1498 1255 565 445 404 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 5135 6847 Size: 274 80 33 51 1443 693 2465 1712 Cuts at: 6847 96 Size: 138 Fragments arranged by size: 2465 1712 1443 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6849 6881 6849 Size: 32 6857 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6889 XbaI T'CTAG_A Cuts at: 3031 4225 3031 Size: 1194 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6889 XmaI C'CCGG_G Cuts at: 1870 3816 4214 1870 Size: 1946 398 4545 Fragments arranged by size: 4545 1946 398 XmnI GAAnn'nnTTC Cuts at: 3513 5018 3513 Size: 1505 5384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4697 276 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI