(Circular) (Six-base) MAPSORT of: pFN21AE1851.seq Check: 7808 from: 1 to: 5815 pFN21AE1851 5815 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3158 1937 Size: 937 284 4594 Fragments arranged by size: 4594 937 284 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3878 5262 5530 1632 Size: 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 268 AfeI AGC'GCT Cuts at: 3035 3035 Size: 5815 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 2459 4714 5691 1203 Size: 1256 2255 977 1327 Fragments arranged by size: 2255 1327 1256 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2111 Size: 32 5783 AlwNI CAG_nnn'CTG Cuts at: 3558 5130 3558 Size: 1572 4243 ApaI G_GGCC'C Cuts at: 2334 2492 2334 Size: 158 5657 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 3124 3350 3502 5611 1493 Size: 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 226 152 AscI GG'CGCG_CC Cuts at: 2941 2941 Size: 5815 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2483 3140 1870 Size: 73 231 309 657 4545 Fragments arranged by size: 4545 657 309 231 73 BamHI G'GATC_C Cuts at: 2235 3145 3512 2235 Size: 910 367 4538 Fragments arranged by size: 4538 910 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2759 3136 4591 618 Size: 325 217 627 972 377 1455 1842 Fragments arranged by size: 1842 1455 972 627 377 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2181 2334 2492 3134 1391 Size: 622 168 153 158 642 4072 Fragments arranged by size: 4072 642 622 168 158 153 BbeI G_GCGC'C Cuts at: 2763 2763 Size: 5815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2841 928 Size: 971 942 3902 Fragments arranged by size: 3902 971 942 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2487 2544 4426 Size: 107 122 71 631 605 815 57 1882 Cuts at: 4426 136 Size: 1525 Fragments arranged by size: 1882 1525 815 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2334 2492 2924 3876 5032 1790 Size: 544 158 432 952 1156 2573 Fragments arranged by size: 2573 1156 952 544 432 158 BmgBI CAC'GTC Cuts at: 1318 3067 1318 Size: 1749 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2512 3546 Size: 416 53 83 399 24 758 337 1034 Cuts at: 3546 4504 442 Size: 958 1753 Fragments arranged by size: 1753 1034 958 758 416 399 337 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 2846 4475 1239 Size: 1071 536 1629 2579 Fragments arranged by size: 2579 1629 1071 536 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4805 5103 3940 Size: 865 298 4652 Fragments arranged by size: 4652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2460 2697 2904 3606 493 Size: 711 1256 237 207 702 2702 Fragments arranged by size: 2702 1256 711 702 237 207 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2212 2760 2914 3622 Size: 53 83 186 936 679 548 154 708 Cuts at: 3622 4004 4639 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 708 679 635 548 382 186 154 83 53 BsaWI w'CCGG_w Cuts at: 2295 4248 4646 4920 5067 5478 2295 Size: 1953 398 274 147 411 2632 Fragments arranged by size: 2632 1953 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2645 2675 1178 Size: 30 165 30 708 30 504 30 4318 Fragments arranged by size: 4318 708 504 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 1186 Size: 933 4882 BseYI C'CCAG_C Cuts at: 2031 5018 5514 2031 Size: 2987 496 2332 Fragments arranged by size: 2987 2332 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 2440 2884 3028 3067 3086 1163 Size: 573 489 215 444 144 39 19 3892 Fragments arranged by size: 3892 573 489 444 215 144 39 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2536 2548 2631 2749 3265 4026 Size: 275 186 535 12 83 118 516 761 Cuts at: 4026 5054 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 535 516 275 186 118 83 12 BsiHKAI G_wGCw'C Cuts at: 3876 3961 5032 3876 Size: 85 1071 4659 Fragments arranged by size: 4659 1071 85 BsiWI C'GTAC_G Cuts at: 2624 2624 Size: 5815 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2181 2334 2492 2924 3134 3876 Size: 399 223 168 153 158 432 210 742 Cuts at: 3876 3961 5032 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 223 210 168 158 153 85 BspEI T'CCGG_A Cuts at: 2295 5478 2295 Size: 3183 2632 BspHI T'CATG_A Cuts at: 2595 3704 5434 2595 Size: 1109 1730 2976 Fragments arranged by size: 2976 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2592 3170 844 Size: 1304 444 578 3489 Fragments arranged by size: 3489 1304 578 444 BsrBI CCG'CTC Cuts at: 1394 1823 2066 3702 1394 Size: 429 243 1636 3507 Fragments arranged by size: 3507 1636 429 243 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2469 4459 4646 1700 Size: 110 659 1990 187 2869 Fragments arranged by size: 2869 1990 659 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 2423 2722 2941 1925 Size: 498 299 219 4799 Fragments arranged by size: 4799 498 299 219 BssSI C'ACGA_G Cuts at: 3104 3875 4887 3104 Size: 771 1012 4032 Fragments arranged by size: 4032 1012 771 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2407 1184 Size: 1223 4592 BstYI r'GATC_y Cuts at: 1972 2235 3145 3512 3897 3914 5366 5452 5464 Size: 263 910 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 910 385 367 346 263 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 2727 2785 513 Size: 552 939 661 62 58 3543 Fragments arranged by size: 3543 939 661 552 62 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2748 487 Size: 683 56 1522 3554 Fragments arranged by size: 3554 1522 683 56 BtsI GCAGTG_nn' Cuts at: 2101 2319 3331 4125 4145 2101 Size: 218 1012 794 20 3771 Fragments arranged by size: 3771 1012 794 218 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 529 Size: 35 1513 35 4232 Fragments arranged by size: 4232 1513 35 35 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DraIII CAC_nnn'GTG Cuts at: 2248 2248 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2098 2533 Size: 54 1425 50 275 18 231 37 435 Cuts at: 2533 2545 3262 4151 5780 8 Size: 12 717 889 1629 43 Fragments arranged by size: 1629 1425 889 717 435 275 231 54 50 43 37 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2533 2545 3262 1537 Size: 275 721 12 717 4090 Fragments arranged by size: 4090 721 717 275 12 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 2546 3745 1353 Size: 992 201 1199 3423 Fragments arranged by size: 3423 1199 992 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2389 2825 4402 4776 4922 387 Size: 1064 138 800 436 1577 374 146 1280 Fragments arranged by size: 1577 1280 1064 800 436 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2846 3878 4475 5262 5530 1239 Size: 393 678 536 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 787 678 597 536 393 268 EcoO109I rG'GnC_Cy Cuts at: 2330 2331 2488 2489 2581 2330 Size: 1 157 1 92 5564 Fragments arranged by size: 5564 157 92 1 1 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FspI TGC'GCA Cuts at: 2693 4321 2693 Size: 1628 4187 FspAI rTGC'GCAy Cuts at: 2693 2693 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 2021 2060 2763 3037 4962 1678 Size: 343 39 703 274 1925 2531 Fragments arranged by size: 2531 1925 703 343 274 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2747 2779 4456 4488 4530 Size: 32 135 32 1175 32 1677 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 1677 1175 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2356 3159 3405 669 Size: 988 281 418 803 246 3079 Fragments arranged by size: 3079 988 803 418 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KasI G'GCGC_C Cuts at: 2759 2759 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4929 5113 1305 Size: 3624 184 2007 Fragments arranged by size: 3624 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3773 4132 4291 518 Size: 648 2607 359 159 2042 Fragments arranged by size: 2607 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2209 2307 2787 2910 3072 3231 3908 Size: 585 18 98 480 123 162 159 677 Cuts at: 3908 5056 5301 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 585 480 245 162 159 123 98 18 NarI GG'CG_CC Cuts at: 2760 2760 Size: 5815 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3173 4718 1132 Size: 97 1944 1545 2229 Fragments arranged by size: 2229 1944 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 2419 1108 Size: 1311 4504 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PmlI CAC'GTG Cuts at: 2460 2697 2460 Size: 237 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 3884 3916 5397 5429 2111 Size: 32 1741 32 1481 32 2497 Fragments arranged by size: 2497 1741 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2581 2581 Size: 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2330 2488 2330 Size: 158 5657 PspXI vC'TCGA_Gb Cuts at: 2174 2174 Size: 5815 PstI C_TGCA'G Cuts at: 830 2190 2265 2516 3167 830 Size: 1360 75 251 651 3478 Fragments arranged by size: 3478 1360 651 251 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2191 3072 2191 Size: 881 4934 SacII CC_GC'GG Cuts at: 2788 2788 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2186 2261 2512 3163 4298 4979 5170 Size: 219 1141 75 251 651 1135 681 191 Cuts at: 5170 826 Size: 1471 Fragments arranged by size: 1471 1141 1135 681 651 251 219 191 75 SfiI GGCCn_nnn'nGGCC Cuts at: 2487 2487 Size: 5815 SfoI GGC'GCC Cuts at: 2761 2761 Size: 5815 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 2485 3142 1872 Size: 613 657 4545 Fragments arranged by size: 4545 657 613 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 3919 4820 5082 820 Size: 197 926 231 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 3254 513 Size: 552 939 1250 3074 Fragments arranged by size: 3074 1250 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 3842 3859 4012 4197 4642 325 Size: 1255 846 1416 17 153 185 445 1498 Fragments arranged by size: 1498 1416 1255 846 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2657 2677 2689 5775 5807 2645 Size: 12 20 12 3086 32 2653 Fragments arranged by size: 3086 2653 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 2870 1316 Size: 1554 4261 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 2174 1943 Size: 231 5584 XmaI C'CCGG_G Cuts at: 1870 2483 3140 1870 Size: 613 657 4545 Fragments arranged by size: 4545 657 613 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI