Restriction map for
pFN21AE1851
(Linear) (Six-base) MAPSORT of: KIEE1851.seq Check: 9857 from: 1 to: 1110
KIEE1851.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 869 1110
Size: 869 241
AfeI AGC'GCT
Cuts at: 0 1030 1110
Size: 1030 80
AflIII A'CryG_T
Cuts at: 0 454 1110
Size: 454 656
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 1110
Size: 106 32 972
Fragments arranged by size:
972 106 32
ApaI G_GGCC'C
Cuts at: 0 329 487 1110
Size: 329 158 623
Fragments arranged by size:
623 329 158
AscI GG'CGCG_CC
Cuts at: 0 936 1110
Size: 936 174
AvaI C'yCGr_G
Cuts at: 0 169 478 1110
Size: 169 309 632
Fragments arranged by size:
632 309 169
BamHI G'GATC_C
Cuts at: 0 230 1110
Size: 230 880
BanI G'GyrC_C
Cuts at: 0 754 1110
Size: 754 356
BanII G_rGCy'C
Cuts at: 0 8 176 329 487 1110
Size: 8 168 153 158 623
Fragments arranged by size:
623 168 158 153 8
BbeI G_GCGC'C
Cuts at: 0 758 1110
Size: 758 352
BbsI GAAGACnn'nnnn_
Cuts at: 0 836 1110
Size: 836 274
BglI GCCn_nnn'nGGC
Cuts at: 0 482 539 1110
Size: 482 57 571
Fragments arranged by size:
571 482 57
Bme1580I G_kGCm'C
Cuts at: 0 329 487 919 1110
Size: 329 158 432 191
Fragments arranged by size:
432 329 191 158
BmgBI CAC'GTC
Cuts at: 0 1062 1110
Size: 1062 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 507 1110
Size: 170 337 603
Fragments arranged by size:
603 337 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 841 1110
Size: 305 536 269
Fragments arranged by size:
536 305 269
BsaAI yAC'GTr
Cuts at: 0 455 692 899 1110
Size: 455 237 207 211
Fragments arranged by size:
455 237 211 207
BsaHI Gr'CG_yC
Cuts at: 0 207 755 909 1110
Size: 207 548 154 201
Fragments arranged by size:
548 207 201 154
BsaWI w'CCGG_w
Cuts at: 0 290 1110
Size: 290 820
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 640 670 1110
Size: 106 30 504 30 440
Fragments arranged by size:
504 440 106 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 1110
Size: 114 996
BseYI C'CCAG_C
Cuts at: 0 26 1110
Size: 26 1084
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 435 879 1023 1062 1081 1110
Size: 220 215 444 144 39 19 29
Fragments arranged by size:
444 220 215 144 39 29 19
BsiEI CG_ry'CG
Cuts at: 0 531 543 626 744 1110
Size: 531 12 83 118 366
Fragments arranged by size:
531 366 118 83 12
BsiWI C'GTAC_G
Cuts at: 0 619 1110
Size: 619 491
Bsp1286I G_dGCh'C
Cuts at: 0 8 176 329 487 919 1110
Size: 8 168 153 158 432 191
Fragments arranged by size:
432 191 168 158 153 8
BspEI T'CCGG_A
Cuts at: 0 290 1110
Size: 290 820
BspHI T'CATG_A
Cuts at: 0 590 1110
Size: 590 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 587 1110
Size: 143 444 523
Fragments arranged by size:
523 444 143
BsrBI CCG'CTC
Cuts at: 0 61 1110
Size: 61 1049
BsrFI r'CCGG_y
Cuts at: 0 464 1110
Size: 464 646
BssHII G'CGCG_C
Cuts at: 0 418 717 936 1110
Size: 418 299 219 174
Fragments arranged by size:
418 299 219 174
BssSI C'ACGA_G
Cuts at: 0 1099 1110
Size: 1099 11
BstEII G'GTnAC_C
Cuts at: 0 402 1110
Size: 402 708
BstYI r'GATC_y
Cuts at: 0 230 1110
Size: 230 880
BtgI C'CryG_G
Cuts at: 0 660 722 780 1110
Size: 660 62 58 330
Fragments arranged by size:
660 330 62 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 743 1110
Size: 743 367
BtsI GCAGTG_nn'
Cuts at: 0 96 314 1110
Size: 96 218 796
Fragments arranged by size:
796 218 96
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1110
Size: 72 35 1003
Fragments arranged by size:
1003 72 35
DraIII CAC_nnn'GTG
Cuts at: 0 243 1110
Size: 243 867
EaeI y'GGCC_r
Cuts at: 0 56 93 528 540 1110
Size: 56 37 435 12 570
Fragments arranged by size:
570 435 56 37 12
EagI C'GGCC_G
Cuts at: 0 528 540 1110
Size: 528 12 570
Fragments arranged by size:
570 528 12
EarI CTCTTCn'nnn_
Cuts at: 0 340 541 1110
Size: 340 201 569
Fragments arranged by size:
569 340 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 384 820 1110
Size: 384 436 290
Fragments arranged by size:
436 384 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 841 1110
Size: 305 536 269
Fragments arranged by size:
536 305 269
EcoO109I rG'GnC_Cy
Cuts at: 0 325 326 483 484 576 1110
Size: 325 1 157 1 92 534
Fragments arranged by size:
534 325 157 92 1 1
FspI TGC'GCA
Cuts at: 0 688 1110
Size: 688 422
FspAI rTGC'GCAy
Cuts at: 0 688 1110
Size: 688 422
HaeII r_GCGC'y
Cuts at: 0 16 55 758 1032 1110
Size: 16 39 703 274 78
Fragments arranged by size:
703 274 78 39 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1110
Size: 742 32 336
Fragments arranged by size:
742 336 32
HincII GTy'rAC
Cuts at: 0 351 1110
Size: 351 759
KasI G'GCGC_C
Cuts at: 0 754 1110
Size: 754 356
MspA1I CmG'CkG
Cuts at: 0 186 204 302 782 905 1067 1110
Size: 186 18 98 480 123 162 43
Fragments arranged by size:
480 186 162 123 98 43 18
NarI GG'CG_CC
Cuts at: 0 755 1110
Size: 755 355
PflMI CCAn_nnn'nTGG
Cuts at: 0 414 1110
Size: 414 696
PmlI CAC'GTG
Cuts at: 0 455 692 1110
Size: 455 237 418
Fragments arranged by size:
455 418 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1110
Size: 106 32 972
Fragments arranged by size:
972 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 576 1110
Size: 576 534
PspOMI G'GGCC_C
Cuts at: 0 325 483 1110
Size: 325 158 627
Fragments arranged by size:
627 325 158
PspXI vC'TCGA_Gb
Cuts at: 0 169 1110
Size: 169 941
PstI C_TGCA'G
Cuts at: 0 185 260 511 1110
Size: 185 75 251 599
Fragments arranged by size:
599 251 185 75
PvuII CAG'CTG
Cuts at: 0 186 1067 1110
Size: 186 881 43
Fragments arranged by size:
881 186 43
SacII CC_GC'GG
Cuts at: 0 783 1110
Size: 783 327
SfcI C'TryA_G
Cuts at: 0 181 256 507 1110
Size: 181 75 251 603
Fragments arranged by size:
603 251 181 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 482 1110
Size: 482 628
SfoI GGC'GCC
Cuts at: 0 756 1110
Size: 756 354
SmaI CCC'GGG
Cuts at: 0 480 1110
Size: 480 630
SmlI C'TyrA_G
Cuts at: 0 169 1110
Size: 169 941
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 1110
Size: 421 689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 652 672 684 1110
Size: 640 12 20 12 426
Fragments arranged by size:
640 426 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 0 865 1110
Size: 865 245
XhoI C'TCGA_G
Cuts at: 0 169 1110
Size: 169 941
XmaI C'CCGG_G
Cuts at: 0 478 1110
Size: 478 632
Enzymes that do cut:
AccI AfeI AflIII AloI ApaI AscI AvaI BamHI
BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI
BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstEII BstYI BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I HincII KasI MspA1I NarI PflMI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacII
SfcI SfiI SfoI SmaI SmlI TaqII TstI Tth111I
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiHKAI BsmI
BsmBI BsrDI BsrGI BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI
FseI HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI XbaI XcmI
XmnI ZraI