################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseRI BsgI BsiEI BsiWI BsoBI BspDI BspLU11I BsrBI BsrDI BsrFI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluNI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 271 GTCTAC GT^[AC][GT]AC AccIII 245 TCCGGA T^CCGGA AcyI 360 GACGCC G[GA]^CG[CT]C AlwNI 325 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 390 GGACC G^G[AT]CC BcnI 15 CCGGG CC^[GC]GG BsaHI 360 GACGCC G[GA]^CG[CT]C BsaI 3 GGTCTC GGTCTC(1/5) BsaJI 573 CCAGGG C^C[ACGT][ACGT]GG BsaWI 245 TCCGGA [AT]^CCGG[AT] BseAI 245 TCCGGA T^CCGGA BsmI 469 GAATGC GAATGC(1/-1) Bsp1407I 74 TGTACA T^GTACA BspEI 245 TCCGGA T^CCGGA BspHI 140 TCATGA T^CATGA BsrGI 74 TGTACA T^GTACA BssHII 578 GCGCGC G^CGCGC Cfr13I 390 GGACC G^G[ACGT]CC DdeI 354 CTCAG C^T[ACGT]AG DraII 389 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 505 CTCTTC CTCTTC(1/4) EcoNI 230 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 389 GGGACCT [GA]G^G[ACGT]CC[CT] HaeII 190 AGCGCC [GA]GCGC^[CT] Hin1I 360 GACGCC G[GA]^CG[CT]C HinfI 242 GACTC G^A[ACGT]TC HphI 342 GGTGA GGTGA(8/7) Ksp632I 505 CTCTTC CTCTTC(1/4) MboII 506 TCTTC GAAGA(8/7) MluI 405 ACGCGT A^CGCGT MroI 245 TCCGGA T^CCGGA MslI 369 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 594 CCGCTG C[AC]G^C[GT]G NciI 15 CCGGG CC^[GC]GG PleI 242 GACTC GAGTC(4/5) PpuMI 389 GGGACCT [GA]G^G[AT]CC[CT] RcaI 140 TCATGA T^CATGA SapI 504 GCTCTTC GCTCTTC(1/4) Sau96I 390 GGACC G^G[ACGT]CC SexAI 434 ACCTGGT A^CC[AT]GGT SmlI 520 CTCAAG C^T[CT][GA]AG SspBI 74 TGTACA T^GTACA TaqI 173 TCGA T^CGA TthHB8I 173 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 405 ACGCGT A^C[GA][CT]GT 425 ACGTGT AluI 295 AGCT AG^CT 515 AGCT Alw44I 154 GTGCAC G^TGCAC 553 GTGCAC ApaLI 154 GTGCAC G^TGCAC 553 GTGCAC AspHI 154 GTGCAC G[AT]GC[AT]^C 553 GTGCAC BbrPI 151 CACGTG CAC^GTG 550 CACGTG BmyI 154 GTGCAC G[AGT]GC[ACT]^C 553 GTGCAC BsiHKAI 154 GTGCAC G[AT]GC[AT]^C 553 GTGCAC BsmBI 334 GAGACG CGTCTC(1/5) 358 GAGACG Bsp1286I 154 GTGCAC G[AGT]GC[ACT]^C 553 GTGCAC BsrI 488 ACTGG ACTGG(1/-1) 66 CCAGT BstAPI 492 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 590 GCAACCGCTGC BstYI 167 AGATCC [GA]^GATC[CT] 326 AGATCC HapII 15 CCGG C^CGG 246 CCGG HpaII 15 CCGG C^CGG 246 CCGG MflI 167 AGATCC [GA]^GATC[CT] 326 AGATCC MspI 15 CCGG C^CGG 246 CCGG NlaIII 141 CATG CATG^ 606 CATG NlaIV 349 GGAACC GG[ACGT]^[ACGT]CC 389 GGGACC PmaCI 151 CACGTG CAC^GTG 550 CACGTG PmlI 151 CACGTG CAC^GTG 550 CACGTG PstI 234 CTGCAG CTGCA^G 394 CTGCAG SfcI 234 CTGCAG C^T[GA][CT]AG 394 CTGCAG XcmI 8 CCACCTACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 365 CCATCATCATCGTGG XhoII 167 AGATCC [GA]^GATC[CT] 326 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AfaI AlwI BbvI BpmI BsaAI BsiYI BslI BsmAI BsmFI BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HgaI HhaI HinP1I ItaI MaeII MboI MnlI MvaI MvnI MwoI NdeII RsaI Sau3AI ScrFI TseI