(Circular) (Six-base) MAPSORT of: pFN21AE4002.seq Check: 4329 from: 1 to: 5314 pFN21AE4002 5314 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2243 2243 Size: 5314 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3124 278 Size: 53 83 186 936 1588 2468 Fragments arranged by size: 2468 1588 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 2657 1937 Size: 340 380 4594 Fragments arranged by size: 4594 380 340 Acc65I G'GTAC_C Cuts at: 2635 2635 Size: 5314 AclI AA'CG_TT Cuts at: 1563 3441 3814 1563 Size: 1878 373 3063 Fragments arranged by size: 3063 1878 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2497 3377 4761 5029 1632 Size: 865 880 1384 268 1917 Fragments arranged by size: 1917 1384 880 865 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5117 AflIII A'CryG_T Cuts at: 1203 2410 2430 4213 5190 1203 Size: 1207 20 1783 977 1327 Fragments arranged by size: 1783 1327 1207 977 20 AgeI A'CCGG_T Cuts at: 4145 4145 Size: 5314 AhdI GACnn_n'nnGTC Cuts at: 4043 4043 Size: 5314 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2251 2573 1184 Size: 1067 322 3925 Fragments arranged by size: 3925 1067 322 AlwNI CAG_nnn'CTG Cuts at: 2335 3057 4629 2335 Size: 722 1572 3020 Fragments arranged by size: 3020 1572 722 ApaLI G'TGCA_C Cuts at: 2159 2558 3371 4527 2159 Size: 399 813 1156 2946 Fragments arranged by size: 2946 1156 813 399 ApoI r'AATT_y Cuts at: 1493 2623 2849 3001 5110 1493 Size: 1130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 226 152 AseI AT'TA_AT Cuts at: 160 1032 3868 160 Size: 872 2836 1606 Fragments arranged by size: 2836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 AvaI C'yCGr_G Cuts at: 1870 1943 2639 1870 Size: 73 696 4545 Fragments arranged by size: 4545 696 73 BamHI G'GATC_C Cuts at: 2644 3011 2644 Size: 367 4947 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 4090 618 Size: 325 217 627 848 1455 1842 Fragments arranged by size: 1842 1455 848 627 325 217 BanII G_rGCy'C Cuts at: 1391 2633 1391 Size: 1242 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3505 3539 1903 Size: 34 1568 34 3678 Fragments arranged by size: 3678 1568 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3206 4416 3206 Size: 1210 4104 BclI T'GATC_A Cuts at: 1548 1548 Size: 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3925 136 Size: 107 122 71 631 605 2253 1525 Fragments arranged by size: 2253 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5309 5309 Size: 5314 BlpI GC'TnA_GC Cuts at: 2731 2731 Size: 5314 Bme1580I G_kGCm'C Cuts at: 1790 2163 2562 3375 4531 1790 Size: 373 399 813 1156 2573 Fragments arranged by size: 2573 1156 813 399 373 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 4003 442 Size: 416 53 83 399 24 1628 958 1753 Fragments arranged by size: 1753 1628 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5314 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2201 2357 3974 1239 Size: 914 48 156 1617 2579 Fragments arranged by size: 2579 1617 914 156 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2510 3439 4304 4602 2510 Size: 929 865 298 3222 Fragments arranged by size: 3222 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 3977 882 Size: 617 515 1963 2219 Fragments arranged by size: 2219 1963 617 515 BsaAI yAC'GTr Cuts at: 493 1204 2158 2470 2557 3105 493 Size: 711 954 312 87 548 2702 Fragments arranged by size: 2702 954 711 548 312 87 BsaBI GATnn'nnATC Cuts at: 5308 5308 Size: 5314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2366 3121 3503 4138 Size: 53 83 186 936 833 755 382 635 Cuts at: 4138 275 Size: 1451 Fragments arranged by size: 1451 936 833 755 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2250 3747 4145 4419 4566 4977 2250 Size: 1497 398 274 147 411 2587 Fragments arranged by size: 2587 1497 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5089 Fragments arranged by size: 5089 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5314 BseYI C'CCAG_C Cuts at: 2032 4517 5013 2032 Size: 2485 496 2333 Fragments arranged by size: 2485 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4741 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 3525 4553 1540 Size: 275 186 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 2562 3375 3460 4531 2163 Size: 399 813 85 1071 2946 Fragments arranged by size: 2946 1071 813 399 85 BsmI GAATG_Cn' Cuts at: 2480 2825 2918 2480 Size: 345 93 4876 Fragments arranged by size: 4876 345 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2333 2357 2333 Size: 24 5290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2562 2633 3375 3460 4531 1391 Size: 399 373 399 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 399 373 85 71 BspEI T'CCGG_A Cuts at: 2250 4977 2250 Size: 2727 2587 BspHI T'CATG_A Cuts at: 2145 3203 4933 2145 Size: 1058 1730 2526 Fragments arranged by size: 2526 1730 1058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2243 2406 2669 844 Size: 1295 104 163 263 3489 Fragments arranged by size: 3489 1295 263 163 104 BsrBI CCG'CTC Cuts at: 1394 1823 3201 1394 Size: 429 1378 3507 Fragments arranged by size: 3507 1378 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3809 3983 65 Size: 1183 2561 174 1396 Fragments arranged by size: 2561 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3958 4145 1700 Size: 110 2148 187 2869 Fragments arranged by size: 2869 2148 187 110 BsrGI T'GTAC_A Cuts at: 96 2079 96 Size: 1983 3331 BssHII G'CGCG_C Cuts at: 1925 2583 1925 Size: 658 4656 BssSI C'ACGA_G Cuts at: 3374 4386 3374 Size: 1012 4302 BstAPI GCAn_nnn'nTGC Cuts at: 794 2503 2601 794 Size: 1709 98 3507 Fragments arranged by size: 3507 1709 98 BstBI TT'CG_AA Cuts at: 4118 4118 Size: 5314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5314 BstYI r'GATC_y Cuts at: 1972 2172 2331 2644 3011 3396 3413 4865 4951 Size: 200 159 313 367 385 17 1452 86 Cuts at: 4951 4963 5309 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 313 200 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3823 Fragments arranged by size: 3823 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4575 Fragments arranged by size: 4575 683 56 BtsI GCAGTG_nn' Cuts at: 2830 3624 3644 2830 Size: 794 20 4500 Fragments arranged by size: 4500 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5279 DraI TTT'AAA Cuts at: 2618 2618 Size: 5314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4321 809 Size: 507 3005 1802 Fragments arranged by size: 3005 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2152 2481 Size: 54 1425 50 275 18 195 127 329 Cuts at: 2481 2761 3650 5279 8 Size: 280 889 1629 43 Fragments arranged by size: 1629 1425 889 329 280 275 195 127 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2761 1537 Size: 275 949 4090 Fragments arranged by size: 4090 949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2516 3244 1353 Size: 1163 728 3423 Fragments arranged by size: 3423 1163 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2312 3901 4275 4421 387 Size: 1064 138 723 1589 374 146 1280 Fragments arranged by size: 1589 1280 1064 723 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2201 2357 2497 3377 3974 4761 Size: 393 521 48 156 140 880 597 787 Cuts at: 4761 5029 1239 Size: 268 1524 Fragments arranged by size: 1524 880 787 597 521 393 268 156 140 48 EcoNI CCTnn'n_nnAGG Cuts at: 2239 2239 Size: 5314 EcoO109I rG'GnC_Cy Cuts at: 2395 2395 Size: 5314 EcoRI G'AATT_C Cuts at: 2623 3001 2623 Size: 378 4936 FspI TGC'GCA Cuts at: 3820 3820 Size: 5314 HaeII r_GCGC'y Cuts at: 1678 2199 4461 1678 Size: 521 2262 2531 Fragments arranged by size: 2531 2262 521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2432 3955 3987 4029 Size: 32 135 32 828 32 1523 32 42 Cuts at: 4029 4061 1373 Size: 32 2626 Fragments arranged by size: 2626 1523 828 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 2904 669 Size: 988 281 720 246 3079 Fragments arranged by size: 3079 988 720 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5314 HpaI GTT'AAC Cuts at: 2904 2904 Size: 5314 KpnI G_GTAC'C Cuts at: 2639 2639 Size: 5314 MfeI C'AATT_G Cuts at: 2913 2913 Size: 5314 MluI A'CGCG_T Cuts at: 2410 5190 2410 Size: 2780 2534 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4428 4612 1305 Size: 3123 184 2007 Fragments arranged by size: 3123 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3492 Fragments arranged by size: 3492 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 3272 3631 3790 518 Size: 648 1212 894 359 159 2042 Fragments arranged by size: 2042 1212 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2601 2730 3407 4555 4800 1606 Size: 995 129 677 1148 245 2120 Fragments arranged by size: 2120 1148 995 677 245 129 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3823 Fragments arranged by size: 3823 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5314 NotI GC'GGCC_GC Cuts at: 2761 2761 Size: 5314 NspI r_CATG'y Cuts at: 1132 1229 2672 4217 1132 Size: 97 1443 1545 2229 Fragments arranged by size: 2229 1545 1443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5314 PciI A'CATG_T Cuts at: 4213 4213 Size: 5314 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5314 PfoI T'CCnGG_A Cuts at: 2217 2334 2217 Size: 117 5197 PmeI GTTT'AAAC Cuts at: 2618 2618 Size: 5314 PmlI CAC'GTG Cuts at: 2158 2557 2158 Size: 399 4915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3415 4896 4928 3383 Size: 32 1481 32 3769 Fragments arranged by size: 3769 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2395 Size: 5314 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5314 PsiI TTA'TAA Cuts at: 2884 2884 Size: 5314 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2072 2104 2072 Size: 32 5282 PstI C_TGCA'G Cuts at: 830 2243 2403 2666 830 Size: 1413 160 263 3478 Fragments arranged by size: 3478 1413 263 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5314 SalI G'TCGA_C Cuts at: 1936 2656 1936 Size: 720 4594 SapI GCTCTTCn'nnn_ Cuts at: 2516 2516 Size: 5314 SbfI CC_TGCA'GG Cuts at: 2666 2666 Size: 5314 ScaI AGT'ACT Cuts at: 3562 5274 3562 Size: 1712 3602 SexAI A'CCwGG_T Cuts at: 2439 2439 Size: 5314 SfcI C'TryA_G Cuts at: 826 1045 2239 2399 2662 3797 4478 4669 826 Size: 219 1194 160 263 1135 681 191 1471 Fragments arranged by size: 1471 1194 1135 681 263 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5314 SmaI CCC'GGG Cuts at: 1872 2641 1872 Size: 769 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 3418 4319 4581 820 Size: 197 926 582 893 901 262 1553 Fragments arranged by size: 1553 926 901 893 582 262 197 SnaBI TAC'GTA Cuts at: 493 3105 493 Size: 2612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5314 SphI G_CATG'C Cuts at: 1132 2672 1132 Size: 1540 3774 SspI AAT'ATT Cuts at: 5 52 3238 5 Size: 47 3186 2081 Fragments arranged by size: 3186 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2753 513 Size: 552 939 749 3074 Fragments arranged by size: 3074 939 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2203 3341 3358 3511 3696 4141 325 Size: 1255 623 1138 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1138 623 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 2487 3560 Size: 274 80 33 51 1443 102 408 1073 Cuts at: 3560 5272 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1073 408 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5274 5306 5274 Size: 32 5282 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5314 XbaI T'CTAG_A Cuts at: 2650 2650 Size: 5314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2377 2020 Size: 357 4957 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5314 XmaI C'CCGG_G Cuts at: 1870 2639 1870 Size: 769 4545 XmnI GAAnn'nnTTC Cuts at: 3443 3443 Size: 5314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3122 276 Size: 53 83 186 936 1588 2468 Fragments arranged by size: 2468 1588 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI