(Circular) (Six-base) MAPSORT of: pFN21AE3447.seq Check: 8843 from: 1 to: 8548 pFN21AE3447 8548 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3017 3100 4195 5538 3017 Size: 83 1095 1343 6027 Fragments arranged by size: 6027 1343 1095 83 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6358 278 Size: 53 83 186 936 4822 2468 Fragments arranged by size: 4822 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 5552 5891 1937 Size: 1698 1917 339 4594 Fragments arranged by size: 4594 1917 1698 339 Acc65I G'GTAC_C Cuts at: 3516 5869 3516 Size: 2353 6195 AclI AA'CG_TT Cuts at: 1563 2550 6675 7048 1563 Size: 987 4125 373 3063 Fragments arranged by size: 4125 3063 987 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3387 3564 3925 5018 5247 6611 7995 8263 Size: 1755 177 361 1093 229 1364 1384 268 Cuts at: 8263 1632 Size: 1917 Fragments arranged by size: 1917 1755 1384 1364 1093 361 268 229 177 AflII C'TTAA_G Cuts at: 820 1017 3164 3533 820 Size: 197 2147 369 5835 Fragments arranged by size: 5835 2147 369 197 AflIII A'CryG_T Cuts at: 1203 3657 3858 3901 7447 8424 1203 Size: 2454 201 43 3546 977 1327 Fragments arranged by size: 3546 2454 1327 977 201 43 AgeI A'CCGG_T Cuts at: 7379 7379 Size: 8548 AhdI GACnn_n'nnGTC Cuts at: 7277 7277 Size: 8548 AleI CACnn'nnGTG Cuts at: 1166 2699 5672 1166 Size: 1533 2973 4042 Fragments arranged by size: 4042 2973 1533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4652 5690 1184 Size: 3468 1038 4042 Fragments arranged by size: 4042 3468 1038 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2876 2908 2876 Size: 32 8516 AlwNI CAG_nnn'CTG Cuts at: 2064 2453 2483 2533 4007 4091 4690 5051 6291 Size: 389 30 50 1474 84 599 361 1240 Cuts at: 6291 7863 2064 Size: 1572 2749 Fragments arranged by size: 2749 1572 1474 1240 599 389 361 84 50 30 ApaI G_GGCC'C Cuts at: 5703 5703 Size: 8548 ApaLI G'TGCA_C Cuts at: 3962 5675 6605 7761 3962 Size: 1713 930 1156 4749 Fragments arranged by size: 4749 1713 1156 930 ApoI r'AATT_y Cuts at: 1493 2407 3065 4482 5857 6083 6235 8344 1493 Size: 914 658 1417 1375 226 152 2109 1697 Fragments arranged by size: 2109 1697 1417 1375 914 658 226 152 AseI AT'TA_AT Cuts at: 160 1032 7102 160 Size: 872 6070 1606 Fragments arranged by size: 6070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8548 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2491 5781 5873 1870 Size: 73 195 353 3290 92 4545 Fragments arranged by size: 4545 3290 353 195 92 73 AvrII C'CTAG_G Cuts at: 2803 2803 Size: 8548 BamHI G'GATC_C Cuts at: 2022 5114 5878 6245 2022 Size: 3092 764 367 4325 Fragments arranged by size: 4325 3092 764 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 3516 4273 4390 5159 Size: 325 217 627 254 1475 757 117 769 Cuts at: 5159 5499 5869 7324 618 Size: 340 370 1455 1842 Fragments arranged by size: 1842 1475 1455 769 757 627 370 340 325 254 217 117 BanII G_rGCy'C Cuts at: 1391 2301 3298 5317 5591 5703 5867 1391 Size: 910 997 2019 274 112 164 4072 Fragments arranged by size: 4072 2019 997 910 274 164 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5769 928 Size: 971 3870 3707 Fragments arranged by size: 3870 3707 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6739 6773 1903 Size: 34 4802 34 3678 Fragments arranged by size: 4802 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5798 6440 7650 5798 Size: 642 1210 6696 Fragments arranged by size: 6696 1210 642 BclI T'GATC_A Cuts at: 1548 3412 1548 Size: 1864 6684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5726 7159 136 Size: 107 122 71 631 605 4054 1433 1525 Fragments arranged by size: 4054 1525 1433 631 605 122 107 71 BglII A'GATC_T Cuts at: 3837 8543 3837 Size: 4706 3842 BlpI GC'TnA_GC Cuts at: 3366 4284 4683 5965 3366 Size: 918 399 1282 5949 Fragments arranged by size: 5949 1282 918 399 Bme1580I G_kGCm'C Cuts at: 1790 3966 4395 5502 5679 5703 6609 7765 1790 Size: 2176 429 1107 177 24 906 1156 2573 Fragments arranged by size: 2573 2176 1156 1107 906 429 177 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3359 4977 5594 Size: 416 53 83 399 24 1942 1618 617 Cuts at: 5594 6279 7237 442 Size: 685 958 1753 Fragments arranged by size: 1942 1753 1618 958 685 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4589 1056 Size: 3533 5015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3578 3665 4113 4488 4615 4752 5028 7208 Size: 2339 87 448 375 127 137 276 2180 Cuts at: 7208 1239 Size: 2579 Fragments arranged by size: 2579 2339 2180 448 375 276 137 127 87 Bpu10I CC'TnA_GC Cuts at: 2939 3621 5237 2939 Size: 682 1616 6250 Fragments arranged by size: 6250 1616 682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 3068 3119 4344 5617 6673 7538 7836 2582 Size: 486 51 1225 1273 1056 865 298 3294 Fragments arranged by size: 3294 1273 1225 1056 865 486 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 7211 882 Size: 617 3738 1974 2219 Fragments arranged by size: 3738 2219 1974 617 BsaAI yAC'GTr Cuts at: 493 1204 5556 6339 493 Size: 711 4352 783 2702 Fragments arranged by size: 4352 2702 783 711 BsaBI GATnn'nnATC Cuts at: 4884 8542 4884 Size: 3658 4890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6355 6737 7372 275 Size: 53 83 186 936 4822 382 635 1451 Fragments arranged by size: 4822 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6981 7379 7653 7800 8211 6981 Size: 398 274 147 411 7318 Fragments arranged by size: 7318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 2876 2906 1178 Size: 30 165 30 697 30 746 30 6820 Fragments arranged by size: 6820 746 697 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2380 2429 2432 2860 5699 1186 Size: 1194 49 3 428 2839 4035 Fragments arranged by size: 4035 2839 1194 428 49 3 BseYI C'CCAG_C Cuts at: 2307 2365 2446 4101 4913 5410 5702 7751 8247 Size: 58 81 1655 812 497 292 2049 496 Cuts at: 8247 2307 Size: 2608 Fragments arranged by size: 2608 2049 1655 812 497 496 292 81 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2645 4065 5191 5355 5565 1163 Size: 573 450 459 1420 1126 164 210 4146 Fragments arranged by size: 4146 1420 1126 573 459 450 210 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2179 4420 4424 5998 6759 7787 Size: 275 186 178 2241 4 1574 761 1028 Cuts at: 7787 1540 Size: 2301 Fragments arranged by size: 2301 2241 1574 1028 761 275 186 178 4 BsiHKAI G_wGCw'C Cuts at: 2852 3266 3298 3966 5098 5317 5679 6609 6694 Size: 414 32 668 1132 219 362 930 85 Cuts at: 6694 7765 2852 Size: 1071 3635 Fragments arranged by size: 3635 1132 1071 930 668 414 362 219 85 32 BsmI GAATG_Cn' Cuts at: 4222 4606 6059 6152 4222 Size: 384 1453 93 6618 Fragments arranged by size: 6618 1453 384 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2852 3266 3298 3966 4395 5098 Size: 399 511 551 414 32 668 429 703 Cuts at: 5098 5317 5502 5591 5679 5703 5867 6609 6694 Size: 219 185 89 88 24 164 742 85 Cuts at: 6694 7765 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 703 668 551 511 429 414 399 219 185 164 89 88 85 32 24 BspEI T'CCGG_A Cuts at: 8211 8211 Size: 8548 BspHI T'CATG_A Cuts at: 6437 8167 6437 Size: 1730 6818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3017 3100 4195 5538 5903 844 Size: 2173 83 1095 1343 365 3489 Fragments arranged by size: 3489 2173 1343 1095 365 83 BsrBI CCG'CTC Cuts at: 1394 1823 6435 1394 Size: 429 4612 3507 Fragments arranged by size: 4612 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4557 5601 7043 7217 65 Size: 1183 3309 1044 1442 174 1396 Fragments arranged by size: 3309 1442 1396 1183 1044 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4415 4419 4423 7192 7379 1700 Size: 110 2605 4 4 2769 187 2869 Fragments arranged by size: 2869 2769 2605 187 110 4 4 BsrGI T'GTAC_A Cuts at: 96 4328 96 Size: 4232 4316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8548 BssSI C'ACGA_G Cuts at: 6608 7620 6608 Size: 1012 7536 BstAPI GCAn_nnn'nTGC Cuts at: 794 2064 4007 4280 4510 794 Size: 1270 1943 273 230 4832 Fragments arranged by size: 4832 1943 1270 273 230 BstBI TT'CG_AA Cuts at: 4745 7352 4745 Size: 2607 5941 BstEII G'GTnAC_C Cuts at: 1184 3315 1184 Size: 2131 6417 BstXI CCAn_nnnn'nTGG Cuts at: 4613 4613 Size: 8548 BstYI r'GATC_y Cuts at: 1972 2022 2760 3502 3837 4153 5114 5878 6245 Size: 50 738 742 335 316 961 764 367 Cuts at: 6245 6630 6647 8099 8185 8197 8543 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 961 764 742 738 385 367 346 335 316 86 50 17 12 BstZ17I GTA'TAC Cuts at: 5553 5553 Size: 8548 Bsu36I CC'TnA_GG Cuts at: 5227 5227 Size: 8548 BtgI C'CryG_G Cuts at: 513 1065 4566 513 Size: 552 3501 4495 Fragments arranged by size: 4495 3501 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7809 Fragments arranged by size: 7809 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2281 2463 2503 2950 3074 4677 6064 6858 Size: 207 182 40 447 124 1603 1387 794 Cuts at: 6858 6878 2074 Size: 20 3744 Fragments arranged by size: 3744 1603 1387 794 447 207 182 124 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8513 DraI TTT'AAA Cuts at: 2049 5852 2049 Size: 3803 4745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7555 809 Size: 507 6239 1802 Fragments arranged by size: 6239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3114 4417 4421 Size: 54 1425 50 275 18 1284 1303 4 Cuts at: 4421 4674 5995 6884 8513 8 Size: 253 1321 889 1629 43 Fragments arranged by size: 1629 1425 1321 1303 1284 889 275 253 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4417 4421 5995 1537 Size: 275 2605 4 1574 4090 Fragments arranged by size: 4090 2605 1574 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3676 4780 4956 5062 6478 1353 Size: 1484 839 1104 176 106 1416 3423 Fragments arranged by size: 3423 1484 1416 1104 839 176 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2198 7135 7509 7655 387 Size: 1064 138 609 4937 374 146 1280 Fragments arranged by size: 4937 1280 1064 609 374 146 138 EcoICRI GAG'CTC Cuts at: 3296 5315 3296 Size: 2019 6529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3387 3564 3578 3665 3925 4113 4488 Size: 393 1755 177 14 87 260 188 375 Cuts at: 4488 4615 4752 5018 5028 5247 6611 7208 7995 Size: 127 137 266 10 219 1364 597 787 Cuts at: 7995 8263 1239 Size: 268 1524 Fragments arranged by size: 1755 1524 1364 787 597 393 375 268 266 260 219 188 177 137 127 87 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2138 Size: 8548 EcoO109I rG'GnC_Cy Cuts at: 2093 2434 5129 5683 2093 Size: 341 2695 554 4958 Fragments arranged by size: 4958 2695 554 341 EcoRI G'AATT_C Cuts at: 5857 6235 5857 Size: 378 8170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3907 3907 Size: 8548 FseI GG_CCGG'CC Cuts at: 4423 4423 Size: 8548 FspI TGC'GCA Cuts at: 4411 7054 4411 Size: 2643 5905 HaeII r_GCGC'y Cuts at: 1678 4430 7695 1678 Size: 2752 3265 2531 Fragments arranged by size: 3265 2752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 3130 3162 3204 Size: 32 135 32 949 32 577 32 42 Cuts at: 3204 3236 3329 3361 3763 3795 3871 3903 4129 Size: 32 93 32 402 32 76 32 226 Cuts at: 4129 4161 5039 5071 5277 5309 7189 7221 7263 Size: 32 878 32 206 32 1880 32 42 Cuts at: 7263 7295 1373 Size: 32 2626 Fragments arranged by size: 2626 1880 949 878 577 402 226 206 135 93 76 42 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2608 5892 6138 669 Size: 988 281 670 3284 246 3079 Fragments arranged by size: 3284 3079 988 670 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8548 HpaI GTT'AAC Cuts at: 6138 6138 Size: 8548 KpnI G_GTAC'C Cuts at: 3520 5873 3520 Size: 2353 6195 MfeI C'AATT_G Cuts at: 3717 4260 6147 3717 Size: 543 1887 6118 Fragments arranged by size: 6118 1887 543 MluI A'CGCG_T Cuts at: 8424 8424 Size: 8548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2000 3009 4186 5408 7662 7846 1305 Size: 695 1009 1177 1222 2254 184 2007 Fragments arranged by size: 2254 2007 1222 1177 1009 695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3116 4676 10 Size: 54 1425 343 1284 1560 3882 Fragments arranged by size: 3882 1560 1425 1284 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 2983 3861 4550 4611 4634 5284 Size: 648 1533 284 878 689 61 23 650 Cuts at: 5284 5672 6506 6865 7024 518 Size: 388 834 359 159 2042 Fragments arranged by size: 2042 1533 878 834 689 650 648 388 359 284 159 61 23 MspA1I CmG'CkG Cuts at: 1606 2200 2230 2483 2727 2746 3452 4007 4280 Size: 594 30 253 244 19 706 555 273 Cuts at: 4280 4448 5812 5964 6641 7789 8034 1606 Size: 168 1364 152 677 1148 245 2120 Fragments arranged by size: 2120 1364 1148 706 677 594 555 273 253 245 244 168 152 30 19 NaeI GCC'GGC Cuts at: 4417 4421 4425 4417 Size: 4 4 8540 Fragments arranged by size: 8540 4 4 NcoI C'CATG_G Cuts at: 513 1065 4566 513 Size: 552 3501 4495 Fragments arranged by size: 4495 3501 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8548 NgoMIV G'CCGG_C Cuts at: 4415 4419 4423 4415 Size: 4 4 8540 Fragments arranged by size: 8540 4 4 NheI G'CTAG_C Cuts at: 1052 4585 1052 Size: 3533 5015 NotI GC'GGCC_GC Cuts at: 5995 5995 Size: 8548 NspI r_CATG'y Cuts at: 1132 1229 3661 3862 3905 5642 5906 7451 1132 Size: 97 2432 201 43 1737 264 1545 2229 Fragments arranged by size: 2432 2229 1737 1545 264 201 97 43 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8548 PciI A'CATG_T Cuts at: 3657 3858 3901 7447 3657 Size: 201 43 3546 4758 Fragments arranged by size: 4758 3546 201 43 PflMI CCAn_nnn'nTGG Cuts at: 1108 4091 1108 Size: 2983 5565 PfoI T'CCnGG_A Cuts at: 4090 4090 Size: 8548 PmeI GTTT'AAAC Cuts at: 5852 5852 Size: 8548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2704 2736 2876 2908 6617 6649 8130 8162 2704 Size: 32 140 32 3709 32 1481 32 3090 Fragments arranged by size: 3709 3090 1481 140 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5683 5683 Size: 8548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8548 PsiI TTA'TAA Cuts at: 4857 6118 4857 Size: 1261 7287 PspOMI G'GGCC_C Cuts at: 5699 5699 Size: 8548 PspXI vC'TCGA_Gb Cuts at: 2138 2138 Size: 8548 PstI C_TGCA'G Cuts at: 830 2069 2276 2458 4003 4458 5647 5900 830 Size: 1239 207 182 1545 455 1189 253 3478 Fragments arranged by size: 3478 1545 1239 1189 455 253 207 182 PvuI CG_AT'CG Cuts at: 2001 2179 2001 Size: 178 8370 PvuII CAG'CTG Cuts at: 2200 2230 2746 3452 4007 4280 2200 Size: 30 516 706 555 273 6468 Fragments arranged by size: 6468 706 555 516 273 30 SacI G_AGCT'C Cuts at: 3298 5317 3298 Size: 2019 6529 SalI G'TCGA_C Cuts at: 1936 5890 1936 Size: 3954 4594 SbfI CC_TGCA'GG Cuts at: 5900 5900 Size: 8548 ScaI AGT'ACT Cuts at: 2169 6796 8508 2169 Size: 4627 1712 2209 Fragments arranged by size: 4627 2209 1712 SexAI A'CCwGG_T Cuts at: 2397 3485 5679 2397 Size: 1088 2194 5266 Fragments arranged by size: 5266 2194 1088 SfcI C'TryA_G Cuts at: 826 1045 2065 2272 2454 3289 3636 3999 4292 Size: 219 1020 207 182 835 347 363 293 Cuts at: 4292 4454 5211 5643 5896 7031 7712 7903 826 Size: 162 757 432 253 1135 681 191 1471 Fragments arranged by size: 1471 1135 1020 835 757 681 432 363 347 293 253 219 207 191 182 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8548 SmaI CCC'GGG Cuts at: 1872 5875 1872 Size: 4003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 2561 3083 3134 3164 3533 Size: 197 926 195 423 522 51 30 369 Cuts at: 3533 4359 5632 6652 7553 7815 820 Size: 826 1273 1020 901 262 1553 Fragments arranged by size: 1553 1273 1020 926 901 826 522 423 369 262 197 195 51 30 SnaBI TAC'GTA Cuts at: 493 5556 6339 493 Size: 5063 783 2702 Fragments arranged by size: 5063 2702 783 SpeI A'CTAG_T Cuts at: 152 152 Size: 8548 SphI G_CATG'C Cuts at: 1132 5642 5906 1132 Size: 4510 264 3774 Fragments arranged by size: 4510 3774 264 SspI AAT'ATT Cuts at: 5 52 2342 6472 5 Size: 47 2290 4130 2081 Fragments arranged by size: 4130 2290 2081 47 StuI AGG'CCT Cuts at: 1517 5225 1517 Size: 3708 4840 StyI C'CwwG_G Cuts at: 513 1065 2803 2853 4566 4863 5003 5987 513 Size: 552 1738 50 1713 297 140 984 3074 Fragments arranged by size: 3074 1738 1713 984 552 297 140 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 3693 4148 5517 5774 6575 6592 Size: 1255 1086 1027 455 1369 257 801 17 Cuts at: 6592 6745 6930 7375 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1369 1255 1086 1027 801 455 445 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 3087 3591 Size: 274 80 33 51 1443 190 920 504 Cuts at: 3591 4328 4450 4623 6794 8506 96 Size: 737 122 173 2171 1712 138 Fragments arranged by size: 2171 1712 1443 920 737 504 274 190 173 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 4221 4253 8508 8540 2100 Size: 32 2089 32 4255 32 2108 Fragments arranged by size: 4255 2108 2089 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8548 XbaI T'CTAG_A Cuts at: 4789 5884 4789 Size: 1095 7453 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2860 5768 2860 Size: 2908 5640 XhoI C'TCGA_G Cuts at: 1943 2138 1943 Size: 195 8353 XmaI C'CCGG_G Cuts at: 1870 5873 1870 Size: 4003 4545 XmnI GAAnn'nnTTC Cuts at: 3306 3459 6677 3306 Size: 153 3218 5177 Fragments arranged by size: 5177 3218 153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6356 276 Size: 53 83 186 936 4822 2468 Fragments arranged by size: 4822 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI