(Linear) (Six-base) MAPSORT of: KIEE3447.seq Check: 2030 from: 1 to: 3843 KIEE3447.orf With 182 enzymes: SgfI * July 3, 2012 17:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1012 1095 2190 3533 3843 Size: 1012 83 1095 1343 310 Fragments arranged by size: 1343 1095 1012 310 83 AccI GT'mk_AC Cuts at: 0 1630 3547 3843 Size: 1630 1917 296 Fragments arranged by size: 1917 1630 296 Acc65I G'GTAC_C Cuts at: 0 1511 3843 Size: 1511 2332 AclI AA'CG_TT Cuts at: 0 545 3843 Size: 545 3298 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1382 1559 1920 3013 3242 3843 Size: 1382 177 361 1093 229 601 Fragments arranged by size: 1382 1093 601 361 229 177 AflII C'TTAA_G Cuts at: 0 1159 1528 3843 Size: 1159 369 2315 Fragments arranged by size: 2315 1159 369 AflIII A'CryG_T Cuts at: 0 1652 1853 1896 3843 Size: 1652 201 43 1947 Fragments arranged by size: 1947 1652 201 43 AleI CACnn'nnGTG Cuts at: 0 694 3667 3843 Size: 694 2973 176 Fragments arranged by size: 2973 694 176 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2647 3685 3843 Size: 2647 1038 158 Fragments arranged by size: 2647 1038 158 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 871 903 3843 Size: 871 32 2940 Fragments arranged by size: 2940 871 32 AlwNI CAG_nnn'CTG Cuts at: 0 59 448 478 528 2002 2086 2685 3046 Size: 59 389 30 50 1474 84 599 361 Cuts at: 3046 3843 Size: 797 Fragments arranged by size: 1474 797 599 389 361 84 59 50 30 ApaI G_GGCC'C Cuts at: 0 3698 3843 Size: 3698 145 ApaLI G'TGCA_C Cuts at: 0 1957 3670 3843 Size: 1957 1713 173 Fragments arranged by size: 1957 1713 173 ApoI r'AATT_y Cuts at: 0 402 1060 2477 3843 Size: 402 658 1417 1366 Fragments arranged by size: 1417 1366 658 402 AvaI C'yCGr_G Cuts at: 0 133 486 3776 3843 Size: 133 353 3290 67 Fragments arranged by size: 3290 353 133 67 AvrII C'CTAG_G Cuts at: 0 798 3843 Size: 798 3045 BamHI G'GATC_C Cuts at: 0 17 3109 3843 Size: 17 3092 734 Fragments arranged by size: 3092 734 17 BanI G'GyrC_C Cuts at: 0 36 1511 2268 2385 3154 3494 3843 Size: 36 1475 757 117 769 340 349 Fragments arranged by size: 1475 769 757 349 340 117 36 BanII G_rGCy'C Cuts at: 0 296 1293 3312 3586 3698 3843 Size: 296 997 2019 274 112 145 Fragments arranged by size: 2019 997 296 274 145 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 3764 3843 Size: 3764 79 BciVI GTATCCnnnnn_n' Cuts at: 0 3793 3843 Size: 3793 50 BclI T'GATC_A Cuts at: 0 1407 3843 Size: 1407 2436 BglI GCCn_nnn'nGGC Cuts at: 0 3721 3843 Size: 3721 122 BglII A'GATC_T Cuts at: 0 1832 3843 Size: 1832 2011 BlpI GC'TnA_GC Cuts at: 0 1361 2279 2678 3843 Size: 1361 918 399 1165 Fragments arranged by size: 1361 1165 918 399 Bme1580I G_kGCm'C Cuts at: 0 1961 2390 3497 3674 3698 3843 Size: 1961 429 1107 177 24 145 Fragments arranged by size: 1961 1107 429 177 145 24 BmrI ACTGGGnnnn_n' Cuts at: 0 1354 2972 3589 3843 Size: 1354 1618 617 254 Fragments arranged by size: 1618 1354 617 254 BmtI G_CTAG'C Cuts at: 0 2584 3843 Size: 2584 1259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1573 1660 2108 2483 2610 2747 3023 3843 Size: 1573 87 448 375 127 137 276 820 Fragments arranged by size: 1573 820 448 375 276 137 127 87 Bpu10I CC'TnA_GC Cuts at: 0 934 1616 3232 3843 Size: 934 682 1616 611 Fragments arranged by size: 1616 934 682 611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 1063 1114 2339 3612 3843 Size: 577 486 51 1225 1273 231 Fragments arranged by size: 1273 1225 577 486 231 51 BsaI GGTCTCn'nnnn_ Cuts at: 0 3232 3843 Size: 3232 611 BsaAI yAC'GTr Cuts at: 0 3551 3843 Size: 3551 292 BsaBI GATnn'nnATC Cuts at: 0 2879 3843 Size: 2879 964 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 95 125 871 901 3843 Size: 95 30 746 30 2942 Fragments arranged by size: 2942 746 95 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 375 424 427 855 3694 3843 Size: 375 49 3 428 2839 149 Fragments arranged by size: 2839 428 375 149 49 3 BseYI C'CCAG_C Cuts at: 0 302 360 441 2096 2908 3405 3697 3843 Size: 302 58 81 1655 812 497 292 146 Fragments arranged by size: 1655 812 497 302 292 146 81 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 640 2060 3186 3350 3560 3843 Size: 181 459 1420 1126 164 210 283 Fragments arranged by size: 1420 1126 459 283 210 181 164 BsiEI CG_ry'CG Cuts at: 0 174 2415 2419 3843 Size: 174 2241 4 1424 Fragments arranged by size: 2241 1424 174 4 BsiHKAI G_wGCw'C Cuts at: 0 847 1261 1293 1961 3093 3312 3674 3843 Size: 847 414 32 668 1132 219 362 169 Fragments arranged by size: 1132 847 668 414 362 219 169 32 BsmI GAATG_Cn' Cuts at: 0 2217 2601 3843 Size: 2217 384 1242 Fragments arranged by size: 2217 1242 384 Bsp1286I G_dGCh'C Cuts at: 0 296 847 1261 1293 1961 2390 3093 3312 Size: 296 551 414 32 668 429 703 219 Cuts at: 3312 3497 3586 3674 3698 3843 Size: 185 89 88 24 145 Fragments arranged by size: 703 668 551 429 414 296 219 185 145 89 88 32 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1012 1095 2190 3533 3843 Size: 1012 83 1095 1343 310 Fragments arranged by size: 1343 1095 1012 310 83 BsrDI GCAATG_nn' Cuts at: 0 2552 3596 3843 Size: 2552 1044 247 Fragments arranged by size: 2552 1044 247 BsrFI r'CCGG_y Cuts at: 0 2410 2414 2418 3843 Size: 2410 4 4 1425 Fragments arranged by size: 2410 1425 4 4 BsrGI T'GTAC_A Cuts at: 0 2323 3843 Size: 2323 1520 BstAPI GCAn_nnn'nTGC Cuts at: 0 59 2002 2275 2505 3843 Size: 59 1943 273 230 1338 Fragments arranged by size: 1943 1338 273 230 59 BstBI TT'CG_AA Cuts at: 0 2740 3843 Size: 2740 1103 BstEII G'GTnAC_C Cuts at: 0 1310 3843 Size: 1310 2533 BstXI CCAn_nnnn'nTGG Cuts at: 0 2608 3843 Size: 2608 1235 BstYI r'GATC_y Cuts at: 0 17 755 1497 1832 2148 3109 3843 Size: 17 738 742 335 316 961 734 Fragments arranged by size: 961 742 738 734 335 316 17 BstZ17I GTA'TAC Cuts at: 0 3548 3843 Size: 3548 295 Bsu36I CC'TnA_GG Cuts at: 0 3222 3843 Size: 3222 621 BtgI C'CryG_G Cuts at: 0 2561 3843 Size: 2561 1282 BtsI GCAGTG_nn' Cuts at: 0 69 276 458 498 945 1069 2672 3843 Size: 69 207 182 40 447 124 1603 1171 Fragments arranged by size: 1603 1171 447 207 182 124 69 40 DraI TTT'AAA Cuts at: 0 44 3843 Size: 44 3799 EaeI y'GGCC_r Cuts at: 0 1109 2412 2416 2669 3843 Size: 1109 1303 4 253 1174 Fragments arranged by size: 1303 1174 1109 253 4 EagI C'GGCC_G Cuts at: 0 2412 2416 3843 Size: 2412 4 1427 Fragments arranged by size: 2412 1427 4 EarI CTCTTCn'nnn_ Cuts at: 0 832 1671 2775 2951 3057 3843 Size: 832 839 1104 176 106 786 Fragments arranged by size: 1104 839 832 786 176 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 193 3843 Size: 193 3650 EcoICRI GAG'CTC Cuts at: 0 1291 3310 3843 Size: 1291 2019 533 Fragments arranged by size: 2019 1291 533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1382 1559 1573 1660 1920 2108 2483 2610 Size: 1382 177 14 87 260 188 375 127 Cuts at: 2610 2747 3013 3023 3242 3843 Size: 137 266 10 219 601 Fragments arranged by size: 1382 601 375 266 260 219 188 177 137 127 87 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 133 3843 Size: 133 3710 EcoO109I rG'GnC_Cy Cuts at: 0 88 429 3124 3678 3843 Size: 88 341 2695 554 165 Fragments arranged by size: 2695 554 341 165 88 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1902 3843 Size: 1902 1941 FseI GG_CCGG'CC Cuts at: 0 2418 3843 Size: 2418 1425 FspI TGC'GCA Cuts at: 0 2406 3843 Size: 2406 1437 HaeII r_GCGC'y Cuts at: 0 2425 3843 Size: 2425 1418 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 516 548 1125 1157 1199 1231 1324 1356 Size: 516 32 577 32 42 32 93 32 Cuts at: 1356 1758 1790 1866 1898 2124 2156 3034 3066 Size: 402 32 76 32 226 32 878 32 Cuts at: 3066 3272 3304 3843 Size: 206 32 539 Fragments arranged by size: 878 577 539 516 402 226 206 93 76 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 603 3843 Size: 603 3240 KpnI G_GTAC'C Cuts at: 0 1515 3843 Size: 1515 2328 MfeI C'AATT_G Cuts at: 0 1712 2255 3843 Size: 1712 543 1588 Fragments arranged by size: 1712 1588 543 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1004 2181 3403 3843 Size: 1004 1177 1222 440 Fragments arranged by size: 1222 1177 1004 440 MscI TGG'CCA Cuts at: 0 1111 2671 3843 Size: 1111 1560 1172 Fragments arranged by size: 1560 1172 1111 MslI CAynn'nnrTG Cuts at: 0 694 978 1856 2545 2606 2629 3279 3667 Size: 694 284 878 689 61 23 650 388 Cuts at: 3667 3843 Size: 176 Fragments arranged by size: 878 694 689 650 388 284 176 61 23 MspA1I CmG'CkG Cuts at: 0 195 225 478 722 741 1447 2002 2275 Size: 195 30 253 244 19 706 555 273 Cuts at: 2275 2443 3807 3843 Size: 168 1364 36 Fragments arranged by size: 1364 706 555 273 253 244 195 168 36 30 19 NaeI GCC'GGC Cuts at: 0 2412 2416 2420 3843 Size: 2412 4 4 1423 Fragments arranged by size: 2412 1423 4 4 NcoI C'CATG_G Cuts at: 0 2561 3843 Size: 2561 1282 NgoMIV G'CCGG_C Cuts at: 0 2410 2414 2418 3843 Size: 2410 4 4 1425 Fragments arranged by size: 2410 1425 4 4 NheI G'CTAG_C Cuts at: 0 2580 3843 Size: 2580 1263 NspI r_CATG'y Cuts at: 0 1656 1857 1900 3637 3843 Size: 1656 201 43 1737 206 Fragments arranged by size: 1737 1656 206 201 43 PciI A'CATG_T Cuts at: 0 1652 1853 1896 3843 Size: 1652 201 43 1947 Fragments arranged by size: 1947 1652 201 43 PflMI CCAn_nnn'nTGG Cuts at: 0 2086 3843 Size: 2086 1757 PfoI T'CCnGG_A Cuts at: 0 2085 3843 Size: 2085 1758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 699 731 871 903 3843 Size: 699 32 140 32 2940 Fragments arranged by size: 2940 699 140 32 32 PpuMI rG'GwC_Cy Cuts at: 0 3678 3843 Size: 3678 165 PsiI TTA'TAA Cuts at: 0 2852 3843 Size: 2852 991 PspOMI G'GGCC_C Cuts at: 0 3694 3843 Size: 3694 149 PspXI vC'TCGA_Gb Cuts at: 0 133 3843 Size: 133 3710 PstI C_TGCA'G Cuts at: 0 64 271 453 1998 2453 3642 3843 Size: 64 207 182 1545 455 1189 201 Fragments arranged by size: 1545 1189 455 207 201 182 64 PvuI CG_AT'CG Cuts at: 0 174 3843 Size: 174 3669 PvuII CAG'CTG Cuts at: 0 195 225 741 1447 2002 2275 3843 Size: 195 30 516 706 555 273 1568 Fragments arranged by size: 1568 706 555 516 273 195 30 SacI G_AGCT'C Cuts at: 0 1293 3312 3843 Size: 1293 2019 531 Fragments arranged by size: 2019 1293 531 ScaI AGT'ACT Cuts at: 0 164 3843 Size: 164 3679 SexAI A'CCwGG_T Cuts at: 0 392 1480 3674 3843 Size: 392 1088 2194 169 Fragments arranged by size: 2194 1088 392 169 SfcI C'TryA_G Cuts at: 0 60 267 449 1284 1631 1994 2287 2449 Size: 60 207 182 835 347 363 293 162 Cuts at: 2449 3206 3638 3843 Size: 757 432 205 Fragments arranged by size: 835 757 432 363 347 293 207 205 182 162 60 SmlI C'TyrA_G Cuts at: 0 133 556 1078 1129 1159 1528 2354 3627 Size: 133 423 522 51 30 369 826 1273 Cuts at: 3627 3843 Size: 216 Fragments arranged by size: 1273 826 522 423 369 216 133 51 30 SnaBI TAC'GTA Cuts at: 0 3551 3843 Size: 3551 292 SphI G_CATG'C Cuts at: 0 3637 3843 Size: 3637 206 SspI AAT'ATT Cuts at: 0 337 3843 Size: 337 3506 StuI AGG'CCT Cuts at: 0 3220 3843 Size: 3220 623 StyI C'CwwG_G Cuts at: 0 798 848 2561 2858 2998 3843 Size: 798 50 1713 297 140 845 Fragments arranged by size: 1713 845 798 297 140 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 661 1688 2143 3512 3769 3843 Size: 661 1027 455 1369 257 74 Fragments arranged by size: 1369 1027 661 455 257 74 TatI w'GTAC_w Cuts at: 0 162 1082 1586 2323 2445 2618 3843 Size: 162 920 504 737 122 173 1225 Fragments arranged by size: 1225 920 737 504 173 162 122 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 95 127 2216 2248 3843 Size: 95 32 2089 32 1595 Fragments arranged by size: 2089 1595 95 32 32 XbaI T'CTAG_A Cuts at: 0 2784 3843 Size: 2784 1059 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 855 3763 3843 Size: 855 2908 80 Fragments arranged by size: 2908 855 80 XhoI C'TCGA_G Cuts at: 0 133 3843 Size: 133 3710 XmnI GAAnn'nnTTC Cuts at: 0 1301 1454 3843 Size: 1301 153 2389 Fragments arranged by size: 2389 1301 153 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII SacI ScaI SexAI SfcI SmlI SnaBI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AfeI AgeI AhdI AscI AseI AsiSI BaeI BbeI BbvCI BcgI BfrBI BmgBI BplI BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BtgZI ClaI CspCI DraIII DrdI EcoRI EcoRV FspAI HindIII HpaI KasI MluI NarI NdeI NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsrI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI SwaI Tth111I XmaI ZraI