################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AcyI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BclI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BspHI BsrBI BsrDI BssHII BssSI Bsu36I CelII CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI Hin1I HpaI KasI KpnI KspI MamI MluI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 3923 TCCGGA T^CCGGA AclI 2992 AACGTT AA^CGTT AflII 2349 CTTAAG C^TTAAG AseI 3049 ATTAAT AT^TAAT AsnI 3049 ATTAAT AT^TAAT AviII 137 TGCGCA TGC^GCA BbvCI 4482 GCTGAGG CCTCAGC(-5/-2) BfrI 2349 CTTAAG C^TTAAG BglI 4049 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1004 AGATCT A^GATCT BsaWI 3923 TCCGGA [AT]^CCGG[AT] BseAI 3923 TCCGGA T^CCGGA BsmBI 1615 CGTCTC CGTCTC(1/5) Bsp1407I 695 TGTACA T^GTACA BspEI 3923 TCCGGA T^CCGGA BsrFI 3661 ACCGGC [GA]^CCGG[CT] BsrGI 695 TGTACA T^GTACA Bst1107I 3081 GTATAC GTA^TAC BstAPI 1018 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4510 TTCGAA TT^CGAA BstEII 775 GGTCACC G^GT[ACGT]ACC BstPI 775 GGTCACC G^GT[ACGT]ACC BstXI 3882 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3081 GTATAC GTA^TAC Cfr10I 3661 ACCGGC [GA]^CCGG[CT] DraI 183 TTTAAA TTT^AAA DrdI 2857 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 775 GGTCACC G^GT[ACGT]ACC EcoRI 1050 GAATTC G^AATTC EcoT22I 1273 ATGCAT ATGCA^T FspI 137 TGCGCA TGC^GCA HaeII 132 GGCGCT [GA]GCGC^[CT] HgaI 1614 GCGTC GACGC(5/10) MfeI 2564 CAATTG C^AATTG MroI 3923 TCCGGA T^CCGGA MunI 2564 CAATTG C^AATTG NheI 1320 GCTAGC G^CTAGC NsiI 1273 ATGCAT ATGCA^T NspV 4510 TTCGAA TT^CGAA PshBI 3049 ATTAAT AT^TAAT Psp1406I 2992 AACGTT AA^CGTT SacI 3398 GAGCTC GAGCT^C SfuI 4510 TTCGAA TT^CGAA SphI 3848 GCATGC GCATG^C SspBI 695 TGTACA T^GTACA SspI 2740 AATATT AAT^ATT StuI 679 AGGCCT AGG^CCT SwaI 182 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 850 GTAGAC GT^[AC][GT]AC 3081 GTATAC AccII 4 CGCG CG^CG 3618 CGCG AflIII 2794 ACATGT A^C[GA][CT]GT 3034 ACATGT ApaI 711 GGGCCC GGGCC^C 4090 GGGCCC Asp700I 432 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1756 GAAATACTTC AvaI 102 CTCGGG C^[CT]CG[GA]G 683 CTCGGG BalI 1985 TGGCCA TGG^CCA 2114 TGGCCA BbsI 213 GAAGAC GAAGAC(2/6) 3574 GAAGAC BcgI 4435 CGATTAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 839 GCAACAATATCG BciVI 2202 GGATAC GTATCC(6/5) 4321 GGATAC BpmI 950 CTGGAG CTGGAG(16/14) 2666 CTGGAG BpuAI 213 GAAGAC GAAGAC(2/6) 3574 GAAGAC BsoBI 102 CTCGGG C^[CT]CG[GA]G 683 CTCGGG BspDI 1475 ATCGAT AT^CGAT 3223 ATCGAT BspLU11I 2794 ACATGT A^CATGT 3034 ACATGT BspMI 4063 ACCTGC ACCTGC(4/8) 4150 ACCTGC BstUI 4 CGCG CG^CG 3618 CGCG ClaI 1475 ATCGAT AT^CGAT 3223 ATCGAT DraIII 699 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3721 CACATTGTG EaeI 1985 TGGCCA [CT]^GGCC[GA] 2114 TGGCCA EcoNI 57 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3591 CCTGTATAAGG FauI 161 CCCGC CCCGC(4/6) 1379 CCCGC MluNI 1985 TGGCCA TGG^CCA 2114 TGGCCA MscI 1985 TGGCCA TGG^CCA 2114 TGGCCA MslI 2497 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4201 CATGGAGGTG MvnI 4 CGCG CG^CG 3618 CGCG PflMI 2106 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3720 CCACATTGTGG SmlI 1312 CTCAAG C^T[CT][GA]AG 2349 CTTAAG SnaBI 1679 TACGTA TAC^GTA 3202 TACGTA Van91I 2106 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3720 CCACATTGTGG XcmI 2102 CCATCCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3683 CCATTTACAGTGTGG XmnI 432 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1756 GAAATACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII