(Linear) (Six-base) MAPSORT of: KIBB0120.seq Check: 6350 from: 1 to: 4587 KIBB0120.orf With 182 enzymes: SgfI * July 3, 2012 15:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4072 4159 4587 Size: 4072 87 428 Fragments arranged by size: 4072 428 87 AccI GT'mk_AC Cuts at: 0 851 3082 4587 Size: 851 2231 1505 Fragments arranged by size: 2231 1505 851 AclI AA'CG_TT Cuts at: 0 2993 4587 Size: 2993 1594 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 328 1207 2373 2543 3496 3506 3541 Size: 235 93 879 1166 170 953 10 35 Cuts at: 3541 4587 Size: 1046 Fragments arranged by size: 1166 1046 953 879 235 170 93 35 10 AflII C'TTAA_G Cuts at: 0 2349 4587 Size: 2349 2238 AflIII A'CryG_T Cuts at: 0 2794 3034 4587 Size: 2794 240 1553 Fragments arranged by size: 2794 1553 240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 836 4171 4418 4587 Size: 836 3335 247 169 Fragments arranged by size: 3335 836 247 169 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1186 1218 4587 Size: 1186 32 3369 Fragments arranged by size: 3369 1186 32 AlwNI CAG_nnn'CTG Cuts at: 0 24 3129 3371 3679 4587 Size: 24 3105 242 308 908 Fragments arranged by size: 3105 908 308 242 24 ApaI G_GGCC'C Cuts at: 0 715 4094 4587 Size: 715 3379 493 Fragments arranged by size: 3379 715 493 ApoI r'AATT_y Cuts at: 0 413 462 760 1050 1423 1630 2710 3486 Size: 413 49 298 290 373 207 1080 776 Cuts at: 3486 3819 4587 Size: 333 768 Fragments arranged by size: 1080 776 768 413 373 333 298 290 207 49 AseI AT'TA_AT Cuts at: 0 3050 4587 Size: 3050 1537 AvaI C'yCGr_G Cuts at: 0 102 683 4587 Size: 102 581 3904 Fragments arranged by size: 3904 581 102 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2195 2228 3192 3225 4587 Size: 2195 33 964 33 1362 Fragments arranged by size: 2195 1362 964 33 33 BanI G'GyrC_C Cuts at: 0 69 1360 3966 4147 4587 Size: 69 1291 2606 181 440 Fragments arranged by size: 2606 1291 440 181 69 BanII G_rGCy'C Cuts at: 0 519 715 3267 3402 4094 4587 Size: 519 196 2552 135 692 493 Fragments arranged by size: 2552 692 519 493 196 135 BbsI GAAGACnn'nnnn_ Cuts at: 0 220 3581 4587 Size: 220 3361 1006 Fragments arranged by size: 3361 1006 220 BbvCI CC'TCA_GC Cuts at: 0 4483 4587 Size: 4483 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 828 862 4424 4458 4587 Size: 828 34 3562 34 129 Fragments arranged by size: 3562 828 129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2196 4315 4587 Size: 2196 2119 272 Fragments arranged by size: 2196 2119 272 BfrBI ATG'CAT Cuts at: 0 1275 4587 Size: 1275 3312 BglI GCCn_nnn'nGGC Cuts at: 0 4055 4587 Size: 4055 532 BglII A'GATC_T Cuts at: 0 1004 4587 Size: 1004 3583 Bme1580I G_kGCm'C Cuts at: 0 85 149 715 1365 3123 4026 4094 4587 Size: 85 64 566 650 1758 903 68 493 Fragments arranged by size: 1758 903 650 566 493 85 68 64 BmrI ACTGGGnnnn_n' Cuts at: 0 1590 2625 4587 Size: 1590 1035 1962 Fragments arranged by size: 1962 1590 1035 BmtI G_CTAG'C Cuts at: 0 1324 4587 Size: 1324 3263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4228 4587 Size: 4228 359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 971 2687 4587 Size: 971 1716 1900 Fragments arranged by size: 1900 1716 971 Bpu10I CC'TnA_GC Cuts at: 0 991 4483 4587 Size: 991 3492 104 Fragments arranged by size: 3492 991 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1297 4587 Size: 1297 3290 BsaI GGTCTCn'nnnn_ Cuts at: 0 874 892 1345 4587 Size: 874 18 453 3242 Fragments arranged by size: 3242 874 453 18 BsaAI yAC'GTr Cuts at: 0 1681 3204 3296 4587 Size: 1681 1523 92 1291 Fragments arranged by size: 1681 1523 1291 92 BsaWI w'CCGG_w Cuts at: 0 3923 4587 Size: 3923 664 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1186 1216 4219 4249 4513 4543 4587 Size: 1186 30 3003 30 264 30 44 Fragments arranged by size: 3003 1186 264 44 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 302 1735 2398 2441 3115 4240 4500 4518 Size: 302 1433 663 43 674 1125 260 18 Cuts at: 4518 4587 Size: 69 Fragments arranged by size: 1433 1125 674 663 302 260 69 43 18 BseYI C'CCAG_C Cuts at: 0 36 2402 4042 4587 Size: 36 2366 1640 545 Fragments arranged by size: 2366 1640 545 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1810 3174 3255 4296 4464 4478 4587 Size: 1810 1364 81 1041 168 14 109 Fragments arranged by size: 1810 1364 1041 168 109 81 14 BsiHKAI G_wGCw'C Cuts at: 0 97 539 1589 3402 4587 Size: 97 442 1050 1813 1185 Fragments arranged by size: 1813 1185 1050 442 97 BsmI GAATG_Cn' Cuts at: 0 292 508 618 1143 4315 4519 4587 Size: 292 216 110 525 3172 204 68 Fragments arranged by size: 3172 525 292 216 204 110 68 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1621 4587 Size: 1621 2966 Bsp1286I G_dGCh'C Cuts at: 0 85 97 149 519 539 715 1365 1589 Size: 85 12 52 370 20 176 650 224 Cuts at: 1589 3123 3267 3402 4026 4094 4587 Size: 1534 144 135 624 68 493 Fragments arranged by size: 1534 650 624 493 370 224 176 144 135 85 68 52 20 12 BspEI T'CCGG_A Cuts at: 0 3923 4587 Size: 3923 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 4072 4159 4587 Size: 4072 87 428 Fragments arranged by size: 4072 428 87 BsrFI r'CCGG_y Cuts at: 0 3661 4587 Size: 3661 926 BsrGI T'GTAC_A Cuts at: 0 695 4587 Size: 695 3892 BstAPI GCAn_nnn'nTGC Cuts at: 0 1024 4587 Size: 1024 3563 BstBI TT'CG_AA Cuts at: 0 4511 4587 Size: 4511 76 BstEII G'GTnAC_C Cuts at: 0 775 4587 Size: 775 3812 BstXI CCAn_nnnn'nTGG Cuts at: 0 3889 4587 Size: 3889 698 BstYI r'GATC_y Cuts at: 0 300 878 1004 1179 1506 4230 4587 Size: 300 578 126 175 327 2724 357 Fragments arranged by size: 2724 578 357 327 300 175 126 BstZ17I GTA'TAC Cuts at: 0 3083 4587 Size: 3083 1504 BtgI C'CryG_G Cuts at: 0 441 3347 3764 3912 4026 4200 4587 Size: 441 2906 417 148 114 174 387 Fragments arranged by size: 2906 441 417 387 174 148 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1215 4587 Size: 1215 3372 BtsI GCAGTG_nn' Cuts at: 0 97 777 813 1601 2608 3213 3267 3287 Size: 97 680 36 788 1007 605 54 20 Cuts at: 3287 4323 4474 4587 Size: 1036 151 113 Fragments arranged by size: 1036 1007 788 680 605 151 113 97 54 36 20 ClaI AT'CG_AT Cuts at: 0 1476 3224 4587 Size: 1476 1748 1363 Fragments arranged by size: 1748 1476 1363 DraI TTT'AAA Cuts at: 0 185 4587 Size: 185 4402 DraIII CAC_nnn'GTG Cuts at: 0 704 3726 4587 Size: 704 3022 861 Fragments arranged by size: 3022 861 704 DrdI GACnn_nn'nnGTC Cuts at: 0 2863 4587 Size: 2863 1724 EaeI y'GGCC_r Cuts at: 0 1985 2114 4587 Size: 1985 129 2473 Fragments arranged by size: 2473 1985 129 EarI CTCTTCn'nnn_ Cuts at: 0 1259 2038 2483 3853 4217 4587 Size: 1259 779 445 1370 364 370 Fragments arranged by size: 1370 1259 779 445 370 364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4512 4587 Size: 4512 75 EcoICRI GAG'CTC Cuts at: 0 3400 4587 Size: 3400 1187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 328 971 1207 2373 2543 2687 3496 Size: 235 93 643 236 1166 170 144 809 Cuts at: 3496 3506 3541 4587 Size: 10 35 1046 Fragments arranged by size: 1166 1046 809 643 236 235 170 144 93 35 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 61 3595 4587 Size: 61 3534 992 Fragments arranged by size: 3534 992 61 EcoO109I rG'GnC_Cy Cuts at: 0 525 712 1241 2725 3126 3885 4090 4091 Size: 525 187 529 1484 401 759 205 1 Cuts at: 4091 4587 Size: 496 Fragments arranged by size: 1484 759 529 525 496 401 205 187 1 EcoRI G'AATT_C Cuts at: 0 1050 4587 Size: 1050 3537 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 452 1260 3129 4587 Size: 452 808 1869 1458 Fragments arranged by size: 1869 1458 808 452 FspI TGC'GCA Cuts at: 0 139 4587 Size: 139 4448 HaeII r_GCGC'y Cuts at: 0 136 4587 Size: 136 4451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 749 781 984 1016 1462 1494 1830 1862 Size: 749 32 203 32 446 32 336 32 Cuts at: 1862 3649 3681 4513 4545 4587 Size: 1787 32 832 32 42 Fragments arranged by size: 1787 832 749 446 336 203 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 453 2814 2991 4587 Size: 453 2361 177 1596 Fragments arranged by size: 2361 1596 453 177 HindIII A'AGCT_T Cuts at: 0 434 973 1219 4587 Size: 434 539 246 3368 Fragments arranged by size: 3368 539 434 246 MfeI C'AATT_G Cuts at: 0 2564 4587 Size: 2564 2023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 686 1622 2345 2606 2636 4587 Size: 686 936 723 261 30 1951 Fragments arranged by size: 1951 936 723 686 261 30 MscI TGG'CCA Cuts at: 0 1987 2116 4587 Size: 1987 129 2471 Fragments arranged by size: 2471 1987 129 MslI CAynn'nnrTG Cuts at: 0 2501 4205 4587 Size: 2501 1704 382 Fragments arranged by size: 2501 1704 382 MspA1I CmG'CkG Cuts at: 0 348 491 3581 4181 4337 4482 4587 Size: 348 143 3090 600 156 145 105 Fragments arranged by size: 3090 600 348 156 145 143 105 NcoI C'CATG_G Cuts at: 0 3347 4026 4200 4587 Size: 3347 679 174 387 Fragments arranged by size: 3347 679 387 174 NdeI CA'TA_TG Cuts at: 0 590 2838 3301 4587 Size: 590 2248 463 1286 Fragments arranged by size: 2248 1286 590 463 NheI G'CTAG_C Cuts at: 0 1320 4587 Size: 1320 3267 NsiI A_TGCA'T Cuts at: 0 1277 4587 Size: 1277 3310 NspI r_CATG'y Cuts at: 0 2601 2798 3038 3065 3852 4037 4587 Size: 2601 197 240 27 787 185 550 Fragments arranged by size: 2601 787 550 240 197 185 27 PasI CC'CwG_GG Cuts at: 0 3889 4587 Size: 3889 698 PciI A'CATG_T Cuts at: 0 2794 3034 4587 Size: 2794 240 1553 Fragments arranged by size: 2794 1553 240 PflMI CCAn_nnn'nTGG Cuts at: 0 2112 3726 4587 Size: 2112 1614 861 Fragments arranged by size: 2112 1614 861 PfoI T'CCnGG_A Cuts at: 0 296 521 948 1580 2105 2664 4587 Size: 296 225 427 632 525 559 1923 Fragments arranged by size: 1923 632 559 525 427 296 225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1186 1218 4587 Size: 1186 32 3369 Fragments arranged by size: 3369 1186 32 PpuMI rG'GwC_Cy Cuts at: 0 525 1241 2725 3126 4587 Size: 525 716 1484 401 1461 Fragments arranged by size: 1484 1461 716 525 401 PsiI TTA'TAA Cuts at: 0 3493 4587 Size: 3493 1094 PspOMI G'GGCC_C Cuts at: 0 711 4090 4587 Size: 711 3379 497 Fragments arranged by size: 3379 711 497 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 731 763 4587 Size: 731 32 3824 Fragments arranged by size: 3824 731 32 PstI C_TGCA'G Cuts at: 0 772 1608 3953 4587 Size: 772 836 2345 634 Fragments arranged by size: 2345 836 772 634 PvuII CAG'CTG Cuts at: 0 348 491 4181 4337 4587 Size: 348 143 3690 156 250 Fragments arranged by size: 3690 348 250 156 143 SacI G_AGCT'C Cuts at: 0 3402 4587 Size: 3402 1185 SfcI C'TryA_G Cuts at: 0 768 834 1604 1928 3949 4176 4587 Size: 768 66 770 324 2021 227 411 Fragments arranged by size: 2021 770 768 411 324 227 66 SmlI C'TyrA_G Cuts at: 0 1312 2349 4587 Size: 1312 1037 2238 Fragments arranged by size: 2238 1312 1037 SnaBI TAC'GTA Cuts at: 0 1681 3204 4587 Size: 1681 1523 1383 Fragments arranged by size: 1681 1523 1383 SphI G_CATG'C Cuts at: 0 3852 4587 Size: 3852 735 SspI AAT'ATT Cuts at: 0 2742 4587 Size: 2742 1845 StuI AGG'CCT Cuts at: 0 681 4587 Size: 681 3906 StyI C'CwwG_G Cuts at: 0 427 1032 1235 2309 3347 3527 3740 4026 Size: 427 605 203 1074 1038 180 213 286 Cuts at: 4026 4125 4200 4587 Size: 99 75 387 Fragments arranged by size: 1074 1038 605 427 387 286 213 203 180 99 75 SwaI ATTT'AAAT Cuts at: 0 185 4587 Size: 185 4402 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2607 3360 4442 4587 Size: 2607 753 1082 145 Fragments arranged by size: 2607 1082 753 145 TatI w'GTAC_w Cuts at: 0 695 1598 2191 4587 Size: 695 903 593 2396 Fragments arranged by size: 2396 903 695 593 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 876 908 2046 2078 3335 3367 4587 Size: 876 32 1138 32 1257 32 1220 Fragments arranged by size: 1257 1220 1138 876 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2109 3690 4587 Size: 2109 1581 897 Fragments arranged by size: 2109 1581 897 XmnI GAAnn'nnTTC Cuts at: 0 436 1760 4587 Size: 436 1324 2827 Fragments arranged by size: 2827 1324 436 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AlfI AloI AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI SfcI SmlI SnaBI SphI SspI StuI StyI SwaI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI AhdI AleI ApaLI AscI AsiSI AvrII BamHI BbeI BclI BlpI BmgBI BsaBI BsaHI BsiEI BsiWI BspHI BsrBI BsrDI BssHII BssSI Bsu36I CspCI EagI EcoRV FseI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NotI NruI PacI PmeI PmlI PshAI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI Tth111I XbaI XhoI XmaI ZraI