################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvrII BbeI BbrPI BbsI BcgI BciVI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 590 ACGTGT A^C[GA][CT]GT AhdI 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1534 CCTGAGG CC^T[ACGT]AGG Asp700I 306 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 478 GGATCC G^GATCC BbvCI 1186 GCTGAGG CCTCAGC(-5/-2) BclI 968 TGATCA T^GATCA BfaI 193 CTAG C^TAG BsaAI 508 TACGTG [CT]AC^GT[GA] BsmBI 814 GAGACG CGTCTC(1/5) BsrBI 1782 CCGCTC CCGCTC(-3/-3) BsrFI 1222 GCCGGC [GA]^CCGG[CT] BssSI 1066 CACGAG CACGAG(-5/-1) BstXI 1788 CCACCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG Cfr10I 1222 GCCGGC [GA]^CCGG[CT] DraIII 1390 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1534 CCTGAGG CC^T[ACGT]AGG FbaI 968 TGATCA T^GATCA MaeI 193 CTAG C^TAG NaeI 1222 GCCGGC GCC^GGC NcoI 1655 CCATGG C^CATGG NgoMIV 1222 GCCGGC G^CCGGC PshAI 1291 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1681 GAGCTC GAGCT^C SapI 1683 GCTCTTC GCTCTTC(1/4) SbfI 351 CCTGCAGG CCTGCA^GG SmaI 1805 CCCGGG CCC^GGG Sse8387I 351 CCTGCAGG CCTGCA^GG StuI 189 AGGCCT AGG^CCT XmaCI 1805 CCCGGG C^CCGGG XmaI 1805 CCCGGG C^CCGGG XmnI 306 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 315 CGCG CG^CG 533 CGCG AcyI 133 GACGCC G[GA]^CG[CT]C 241 GACGCC AfaI 341 GTAC GT^AC 660 GTAC AviII 1055 TGCGCA TGC^GCA 1097 TGCGCA BanI 1254 GGCACC G^G[CT][GA]CC 1841 GGCACC BglI 724 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1442 GCCTGGCAGGC BsaHI 133 GACGCC G[GA]^CG[CT]C 241 GACGCC BseRI 1189 GAGGAG GAGGAG(10/8) 1246 GAGGAG BstAPI 177 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1047 GCAGCAGCTGC BstUI 315 CGCG CG^CG 533 CGCG DrdI 984 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1354 GACATGATGGTC DsaI 1655 CCATGG C^C[GA][CT]GG 1756 CCACGG EarI 1684 CTCTTC CTCTTC(1/4) 202 GAAGAG EcoT14I 77 CCAAGG C^C[AT][AT]GG 1655 CCATGG FspI 1055 TGCGCA TGC^GCA 1097 TGCGCA HaeII 836 AGCGCC [GA]GCGC^[CT] 927 GGCGCT Hin1I 133 GACGCC G[GA]^CG[CT]C 241 GACGCC Ksp632I 1684 CTCTTC CTCTTC(1/4) 202 GAAGAG MvnI 315 CGCG CG^CG 533 CGCG PflMI 77 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 773 CCAACCCCTGG PpuMI 58 GGGACCT [GA]G^G[AT]CC[CT] 1165 GGGACCC RsaI 341 GTAC GT^AC 660 GTAC SphI 182 GCATGC GCATG^C 977 GCATGC StyI 77 CCAAGG C^C[AT][AT]GG 1655 CCATGG Van91I 77 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 773 CCAACCCCTGG XcmI 1139 CCACGCCCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1274 CCATCGACCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I XhoII