(Circular) (Six-base) MAPSORT of: pFN21AE0987.seq Check: 9504 from: 1 to: 6553 pFN21AE0987 6553 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 3487 2350 Size: 1137 5416 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3896 1937 Size: 1959 4594 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2226 2443 3490 4616 6000 6268 1632 Size: 560 34 217 1047 1126 1384 268 1917 Fragments arranged by size: 1917 1384 1126 1047 560 268 217 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 2595 5452 6429 1203 Size: 1392 2857 977 1327 Fragments arranged by size: 2857 1392 1327 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 2599 5282 2599 Size: 2683 3870 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2870 1184 Size: 1686 4867 AlwNI CAG_nnn'CTG Cuts at: 2701 3058 3555 3570 3683 3705 4296 5868 2701 Size: 357 497 15 113 22 591 1572 3386 Fragments arranged by size: 3386 1572 591 497 357 113 22 15 ApaI G_GGCC'C Cuts at: 2554 3416 3642 3761 2554 Size: 862 226 119 5346 Fragments arranged by size: 5346 862 226 119 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 3862 4088 4240 6349 1493 Size: 2369 226 152 2109 1697 Fragments arranged by size: 2369 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 3409 3590 3810 3878 1870 Size: 73 1466 181 220 68 4545 Fragments arranged by size: 4545 1466 220 181 73 68 BamHI G'GATC_C Cuts at: 2483 3883 4250 2483 Size: 1400 367 4786 Fragments arranged by size: 4786 1400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3259 3846 3874 5329 618 Size: 325 217 627 1472 587 28 1455 1842 Fragments arranged by size: 1842 1472 1455 627 587 325 217 28 BanII G_rGCy'C Cuts at: 1391 2554 3416 3423 3642 3690 3761 3769 3777 Size: 1163 862 7 219 48 71 8 8 Cuts at: 3777 3872 1391 Size: 95 4072 Fragments arranged by size: 4072 1163 862 219 95 71 48 8 8 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5582 BbvCI CC'TCA_GC Cuts at: 3192 3192 Size: 6553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 1903 Size: 34 2807 34 3678 Fragments arranged by size: 3678 2807 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4445 5655 4445 Size: 1210 5343 BclI T'GATC_A Cuts at: 1548 2973 1548 Size: 1425 5128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2735 3453 5164 Size: 107 122 71 631 605 1063 718 1711 Cuts at: 5164 136 Size: 1525 Fragments arranged by size: 1711 1525 1063 718 631 605 122 107 71 BglII A'GATC_T Cuts at: 6548 6548 Size: 6553 BlpI GC'TnA_GC Cuts at: 2622 3524 3603 3970 2622 Size: 902 79 367 5205 Fragments arranged by size: 5205 902 367 79 Bme1580I G_kGCm'C Cuts at: 1790 2554 3070 3405 3416 3642 3761 3849 4614 Size: 764 516 335 11 226 119 88 765 Cuts at: 4614 5770 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 765 764 516 335 226 119 88 11 BmgBI CAC'GTC Cuts at: 1318 2218 2596 1318 Size: 900 378 5275 Fragments arranged by size: 5275 900 378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 4284 5242 Size: 416 53 83 399 24 1595 1272 958 Cuts at: 5242 442 Size: 1753 Fragments arranged by size: 1753 1595 1272 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3605 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 3732 3764 3815 5213 1239 Size: 1061 1432 32 51 1398 2579 Fragments arranged by size: 2579 1432 1398 1061 51 32 Bpu10I CC'TnA_GC Cuts at: 3192 3417 3684 3768 3192 Size: 225 267 84 5977 Fragments arranged by size: 5977 267 225 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 2237 2252 2369 2399 2777 2843 2939 3359 Size: 123 15 117 30 378 66 96 420 Cuts at: 3359 4678 5543 5841 2114 Size: 1319 865 298 2826 Fragments arranged by size: 2826 1319 865 420 378 298 123 117 96 66 30 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3068 3329 3358 3538 5216 882 Size: 617 1569 261 29 180 1678 2219 Fragments arranged by size: 2219 1678 1569 617 261 180 29 BsaAI yAC'GTr Cuts at: 493 1204 2515 4344 493 Size: 711 1311 1829 2702 Fragments arranged by size: 2702 1829 1311 711 BsaBI GATnn'nnATC Cuts at: 6547 6547 Size: 6553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2247 4360 4742 Size: 53 83 186 936 606 108 2113 382 Cuts at: 4742 5377 275 Size: 635 1451 Fragments arranged by size: 2113 1451 936 635 606 382 186 108 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6328 Fragments arranged by size: 6328 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3209 3266 1186 Size: 2023 57 4473 Fragments arranged by size: 4473 2023 57 BseYI C'CCAG_C Cuts at: 2566 3314 3570 3698 5756 6252 2566 Size: 748 256 128 2058 496 2867 Fragments arranged by size: 2867 2058 748 496 256 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2612 3325 3721 1163 Size: 573 429 447 713 396 3995 Fragments arranged by size: 3995 713 573 447 429 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2102 2367 2946 3690 4614 4699 5770 2102 Size: 265 579 744 924 85 1071 2885 Fragments arranged by size: 2885 1071 924 744 579 265 85 BsmI GAATG_Cn' Cuts at: 4064 4157 4064 Size: 93 6460 BsmBI CGTCTCn'nnnn_ Cuts at: 2813 2813 Size: 6553 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2367 2554 2946 3070 3405 3416 Size: 399 312 265 187 392 124 335 11 Cuts at: 3416 3423 3642 3690 3761 3769 3777 3849 3872 Size: 7 219 48 71 8 8 72 23 Cuts at: 3872 4614 4699 5770 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 335 312 265 219 187 124 85 72 71 48 23 11 8 8 7 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 3347 3487 3908 844 Size: 1506 997 140 421 3489 Fragments arranged by size: 3489 1506 997 421 140 BsrBI CCG'CTC Cuts at: 1394 1823 3789 4440 1394 Size: 429 1966 651 3507 Fragments arranged by size: 3507 1966 651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5048 5222 65 Size: 1183 3800 174 1396 Fragments arranged by size: 3800 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3227 5197 5384 1700 Size: 110 1417 1970 187 2869 Fragments arranged by size: 2869 1970 1417 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 3071 4613 5625 3071 Size: 1542 1012 3999 Fragments arranged by size: 3999 1542 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2188 3058 794 Size: 1394 870 4289 Fragments arranged by size: 4289 1394 870 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstXI CCAn_nnnn'nTGG Cuts at: 3800 3800 Size: 6553 BstYI r'GATC_y Cuts at: 1972 2124 2483 2559 3883 4250 4635 4652 6104 Size: 152 359 76 1324 367 385 17 1452 Cuts at: 6104 6190 6202 6548 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1324 385 367 359 346 152 86 76 17 12 Bsu36I CC'TnA_GG Cuts at: 3540 3540 Size: 6553 BtgI C'CryG_G Cuts at: 513 1065 3660 3761 513 Size: 552 2595 101 3305 Fragments arranged by size: 3305 2595 552 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5814 Fragments arranged by size: 5814 683 56 BtsI GCAGTG_nn' Cuts at: 2182 2902 3070 3226 4069 4863 4883 2182 Size: 720 168 156 843 794 20 3852 Fragments arranged by size: 3852 843 794 720 168 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2700 2735 529 Size: 35 2136 35 4347 Fragments arranged by size: 4347 2136 35 35 DraI TTT'AAA Cuts at: 3857 3857 Size: 6553 DraIII CAC_nnn'GTG Cuts at: 3400 3400 Size: 6553 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2995 3365 5560 809 Size: 507 1679 370 2195 1802 Fragments arranged by size: 2195 1802 1679 507 370 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2814 2901 Size: 54 1425 50 275 18 285 699 87 Cuts at: 2901 3156 3225 3391 3612 4000 4889 6518 8 Size: 255 69 166 221 388 889 1629 43 Fragments arranged by size: 1629 1425 889 699 388 285 275 255 221 166 87 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 3695 4483 1353 Size: 849 1493 788 3423 Fragments arranged by size: 3423 1493 849 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 2476 2954 5140 5514 5660 Size: 1064 138 521 366 478 2186 374 146 Cuts at: 5660 387 Size: 1280 Fragments arranged by size: 2186 1280 1064 521 478 374 366 146 138 EcoICRI GAG'CTC Cuts at: 3688 3688 Size: 6553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2226 2300 2443 3490 3732 3764 Size: 393 560 34 74 143 1047 242 32 Cuts at: 3764 3815 4616 5213 6000 6268 1239 Size: 51 801 597 787 268 1524 Fragments arranged by size: 1524 1047 801 787 597 560 393 268 242 143 74 51 34 32 EcoO109I rG'GnC_Cy Cuts at: 2064 2551 2698 3171 3413 3552 3594 3757 2064 Size: 487 147 473 242 139 42 163 4860 Fragments arranged by size: 4860 487 473 242 163 147 139 42 EcoRI G'AATT_C Cuts at: 3862 4240 3862 Size: 378 6175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 6553 FspI TGC'GCA Cuts at: 3062 3104 5059 3062 Size: 42 1955 4556 Fragments arranged by size: 4556 1955 42 HaeII r_GCGC'y Cuts at: 1678 2845 2936 5700 1678 Size: 1167 91 2764 2531 Fragments arranged by size: 2764 2531 1167 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2585 2617 5194 Size: 32 135 32 896 32 85 32 2577 Cuts at: 5194 5226 5268 5300 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2577 896 135 85 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3897 4143 669 Size: 988 281 1959 246 3079 Fragments arranged by size: 3079 1959 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6553 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 2847 3381 3519 3525 5667 5851 1305 Size: 696 846 534 138 6 2142 184 2007 Fragments arranged by size: 2142 2007 846 696 534 184 138 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2903 3393 3614 10 Size: 54 1425 343 285 786 490 221 2949 Fragments arranged by size: 2949 1425 786 490 343 285 221 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3659 3798 4511 4870 5029 518 Size: 648 1269 1224 139 713 359 159 2042 Fragments arranged by size: 2042 1269 1224 713 648 359 159 139 MspA1I CmG'CkG Cuts at: 1606 2451 3058 3289 3570 3705 3969 4646 5794 Size: 845 607 231 281 135 264 677 1148 Cuts at: 5794 6039 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 845 677 607 281 264 245 231 135 NaeI GCC'GGC Cuts at: 3229 3229 Size: 6553 NcoI C'CATG_G Cuts at: 513 1065 3660 513 Size: 552 2595 3406 Fragments arranged by size: 3406 2595 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NgoMIV G'CCGG_C Cuts at: 3227 3227 Size: 6553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2191 2986 3839 3911 5456 1132 Size: 97 962 795 853 72 1545 2229 Fragments arranged by size: 2229 1545 962 853 795 97 72 PasI CC'CwG_GG Cuts at: 1348 2605 2695 1348 Size: 1257 90 5206 Fragments arranged by size: 5206 1257 90 PciI A'CATG_T Cuts at: 5452 5452 Size: 6553 PflMI CCAn_nnn'nTGG Cuts at: 1108 2088 2784 1108 Size: 980 696 4877 Fragments arranged by size: 4877 980 696 PfoI T'CCnGG_A Cuts at: 3709 3709 Size: 6553 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 3171 2064 Size: 1107 5446 PshAI GACnn'nnGTC Cuts at: 1613 3300 1613 Size: 1687 4866 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PspOMI G'GGCC_C Cuts at: 2550 3412 3638 3757 2550 Size: 862 226 119 5346 Fragments arranged by size: 5346 862 226 119 PstI C_TGCA'G Cuts at: 830 2361 3309 3624 3905 830 Size: 1531 948 315 281 3478 Fragments arranged by size: 3478 1531 948 315 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 3058 3289 3570 3705 3058 Size: 231 281 135 5906 Fragments arranged by size: 5906 281 231 135 SacI G_AGCT'C Cuts at: 3690 3690 Size: 6553 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SanDI GG'GwC_CC Cuts at: 3171 3171 Size: 6553 SapI GCTCTTCn'nnn_ Cuts at: 3695 3695 Size: 6553 SbfI CC_TGCA'GG Cuts at: 2361 3905 2361 Size: 1544 5009 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SexAI A'CCwGG_T Cuts at: 2262 2670 3262 2262 Size: 408 592 5553 Fragments arranged by size: 5553 592 408 SfcI C'TryA_G Cuts at: 826 1045 2357 3305 3436 3620 3901 5036 5717 Size: 219 1312 948 131 184 281 1135 681 Cuts at: 5717 5908 826 Size: 191 1471 Fragments arranged by size: 1471 1312 1135 948 681 281 219 191 184 131 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3812 3880 1872 Size: 1940 68 4545 Fragments arranged by size: 4545 1940 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2231 2252 2384 2414 2792 Size: 197 926 186 102 21 132 30 378 Cuts at: 2792 2858 2954 3374 4657 5558 5820 820 Size: 66 96 420 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 901 420 378 262 197 186 132 102 96 66 30 21 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 2191 2986 3911 1132 Size: 1059 795 925 3774 Fragments arranged by size: 3774 1059 925 795 SspI AAT'ATT Cuts at: 5 52 4477 5 Size: 47 4425 2081 Fragments arranged by size: 4425 2081 47 StuI AGG'CCT Cuts at: 1517 2196 1517 Size: 679 5874 StyI C'CwwG_G Cuts at: 513 1065 2082 3660 3992 513 Size: 552 1017 1578 332 3074 Fragments arranged by size: 3074 1578 1017 552 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 4580 4597 4750 4935 5380 325 Size: 1255 1515 1485 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1485 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4799 6511 96 Size: 274 80 33 51 1443 2822 1712 138 Fragments arranged by size: 2822 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3600 3632 6513 6545 3600 Size: 32 2881 32 3608 Fragments arranged by size: 3608 2881 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3151 3286 3151 Size: 135 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6553 XmaI C'CCGG_G Cuts at: 1870 3810 3878 1870 Size: 1940 68 4545 Fragments arranged by size: 4545 1940 68 XmnI GAAnn'nnTTC Cuts at: 2315 4682 2315 Size: 2367 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI