(Linear) (Six-base) MAPSORT of: KIEE0987.seq Check: 7509 from: 1 to: 1848 KIEE0987.orf With 182 enzymes: SgfI * July 3, 2012 16:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 345 1482 1848 Size: 345 1137 366 Fragments arranged by size: 1137 366 345 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 221 438 1485 1848 Size: 187 34 217 1047 363 Fragments arranged by size: 1047 363 217 187 34 AflIII A'CryG_T Cuts at: 0 590 1848 Size: 590 1258 AhdI GACnn_n'nnGTC Cuts at: 0 594 1848 Size: 594 1254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 865 1848 Size: 865 983 AlwNI CAG_nnn'CTG Cuts at: 0 696 1053 1550 1565 1678 1700 1848 Size: 696 357 497 15 113 22 148 Fragments arranged by size: 696 497 357 148 113 22 15 ApaI G_GGCC'C Cuts at: 0 549 1411 1637 1756 1848 Size: 549 862 226 119 92 Fragments arranged by size: 862 549 226 119 92 AvaI C'yCGr_G Cuts at: 0 1404 1585 1805 1848 Size: 1404 181 220 43 Fragments arranged by size: 1404 220 181 43 BamHI G'GATC_C Cuts at: 0 478 1848 Size: 478 1370 BanI G'GyrC_C Cuts at: 0 1254 1841 1848 Size: 1254 587 7 Fragments arranged by size: 1254 587 7 BanII G_rGCy'C Cuts at: 0 549 1411 1418 1637 1685 1756 1764 1772 Size: 549 862 7 219 48 71 8 8 Cuts at: 1772 1848 Size: 76 Fragments arranged by size: 862 549 219 76 71 48 8 8 7 BbvCI CC'TCA_GC Cuts at: 0 1187 1848 Size: 1187 661 BclI T'GATC_A Cuts at: 0 968 1848 Size: 968 880 BglI GCCn_nnn'nGGC Cuts at: 0 730 1448 1848 Size: 730 718 400 Fragments arranged by size: 730 718 400 BlpI GC'TnA_GC Cuts at: 0 617 1519 1598 1848 Size: 617 902 79 250 Fragments arranged by size: 902 617 250 79 Bme1580I G_kGCm'C Cuts at: 0 549 1065 1400 1411 1637 1756 1844 1848 Size: 549 516 335 11 226 119 88 4 Fragments arranged by size: 549 516 335 226 119 88 11 4 BmgBI CAC'GTC Cuts at: 0 213 591 1848 Size: 213 378 1257 Fragments arranged by size: 1257 378 213 BmrI ACTGGGnnnn_n' Cuts at: 0 1007 1848 Size: 1007 841 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1600 1848 Size: 1600 248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1727 1759 1810 1848 Size: 295 1432 32 51 38 Fragments arranged by size: 1432 295 51 38 32 Bpu10I CC'TnA_GC Cuts at: 0 1187 1412 1679 1763 1848 Size: 1187 225 267 84 85 Fragments arranged by size: 1187 267 225 85 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 232 247 364 394 772 838 934 Size: 109 123 15 117 30 378 66 96 Cuts at: 934 1354 1848 Size: 420 494 Fragments arranged by size: 494 420 378 123 117 109 96 66 30 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 1063 1324 1353 1533 1848 Size: 1063 261 29 180 315 Fragments arranged by size: 1063 315 261 180 29 BsaAI yAC'GTr Cuts at: 0 510 1848 Size: 510 1338 BsaHI Gr'CG_yC Cuts at: 0 134 242 1848 Size: 134 108 1606 Fragments arranged by size: 1606 134 108 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1204 1261 1848 Size: 1204 57 587 Fragments arranged by size: 1204 587 57 BseYI C'CCAG_C Cuts at: 0 561 1309 1565 1693 1848 Size: 561 748 256 128 155 Fragments arranged by size: 748 561 256 155 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 607 1320 1716 1848 Size: 160 447 713 396 132 Fragments arranged by size: 713 447 396 160 132 BsiHKAI G_wGCw'C Cuts at: 0 97 362 941 1685 1848 Size: 97 265 579 744 163 Fragments arranged by size: 744 579 265 163 97 BsmBI CGTCTCn'nnnn_ Cuts at: 0 808 1848 Size: 808 1040 Bsp1286I G_dGCh'C Cuts at: 0 97 362 549 941 1065 1400 1411 1418 Size: 97 265 187 392 124 335 11 7 Cuts at: 1418 1637 1685 1756 1764 1772 1844 1848 Size: 219 48 71 8 8 72 4 Fragments arranged by size: 392 335 265 219 187 124 97 72 71 48 11 8 8 7 4 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 345 1342 1482 1848 Size: 345 997 140 366 Fragments arranged by size: 997 366 345 140 BsrBI CCG'CTC Cuts at: 0 1784 1848 Size: 1784 64 BsrFI r'CCGG_y Cuts at: 0 1222 1848 Size: 1222 626 BssSI C'ACGA_G Cuts at: 0 1066 1848 Size: 1066 782 BstAPI GCAn_nnn'nTGC Cuts at: 0 183 1053 1848 Size: 183 870 795 Fragments arranged by size: 870 795 183 BstXI CCAn_nnnn'nTGG Cuts at: 0 1795 1848 Size: 1795 53 BstYI r'GATC_y Cuts at: 0 119 478 554 1848 Size: 119 359 76 1294 Fragments arranged by size: 1294 359 119 76 Bsu36I CC'TnA_GG Cuts at: 0 1535 1848 Size: 1535 313 BtgI C'CryG_G Cuts at: 0 1655 1756 1848 Size: 1655 101 92 Fragments arranged by size: 1655 101 92 BtsI GCAGTG_nn' Cuts at: 0 177 897 1065 1221 1848 Size: 177 720 168 156 627 Fragments arranged by size: 720 627 177 168 156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 695 730 1848 Size: 695 35 1118 Fragments arranged by size: 1118 695 35 DraIII CAC_nnn'GTG Cuts at: 0 1395 1848 Size: 1395 453 DrdI GACnn_nn'nnGTC Cuts at: 0 990 1360 1848 Size: 990 370 488 Fragments arranged by size: 990 488 370 EaeI y'GGCC_r Cuts at: 0 110 809 896 1151 1220 1386 1607 1848 Size: 110 699 87 255 69 166 221 241 Fragments arranged by size: 699 255 241 221 166 110 87 69 EarI CTCTTCn'nnn_ Cuts at: 0 197 1690 1848 Size: 197 1493 158 Fragments arranged by size: 1493 197 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 105 471 949 1848 Size: 105 366 478 899 Fragments arranged by size: 899 478 366 105 EcoICRI GAG'CTC Cuts at: 0 1683 1848 Size: 1683 165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 221 295 438 1485 1727 1759 1810 Size: 187 34 74 143 1047 242 32 51 Cuts at: 1810 1848 Size: 38 Fragments arranged by size: 1047 242 187 143 74 51 38 34 32 EcoO109I rG'GnC_Cy Cuts at: 0 59 546 693 1166 1408 1547 1589 1752 Size: 59 487 147 473 242 139 42 163 Cuts at: 1752 1848 Size: 96 Fragments arranged by size: 487 473 242 163 147 139 96 59 42 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 78 1848 Size: 78 1770 FspI TGC'GCA Cuts at: 0 1057 1099 1848 Size: 1057 42 749 Fragments arranged by size: 1057 749 42 HaeII r_GCGC'y Cuts at: 0 840 931 1848 Size: 840 91 917 Fragments arranged by size: 917 840 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 580 612 1848 Size: 463 32 85 32 1236 Fragments arranged by size: 1236 463 85 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 842 1376 1514 1520 1848 Size: 842 534 138 6 328 Fragments arranged by size: 842 534 328 138 6 MscI TGG'CCA Cuts at: 0 112 898 1388 1609 1848 Size: 112 786 490 221 239 Fragments arranged by size: 786 490 239 221 112 MslI CAynn'nnrTG Cuts at: 0 430 1654 1793 1848 Size: 430 1224 139 55 Fragments arranged by size: 1224 430 139 55 MspA1I CmG'CkG Cuts at: 0 446 1053 1284 1565 1700 1848 Size: 446 607 231 281 135 148 Fragments arranged by size: 607 446 281 231 148 135 NaeI GCC'GGC Cuts at: 0 1224 1848 Size: 1224 624 NcoI C'CATG_G Cuts at: 0 1655 1848 Size: 1655 193 NgoMIV G'CCGG_C Cuts at: 0 1222 1848 Size: 1222 626 NspI r_CATG'y Cuts at: 0 186 981 1834 1848 Size: 186 795 853 14 Fragments arranged by size: 853 795 186 14 PasI CC'CwG_GG Cuts at: 0 600 690 1848 Size: 600 90 1158 Fragments arranged by size: 1158 600 90 PflMI CCAn_nnn'nTGG Cuts at: 0 83 779 1848 Size: 83 696 1069 Fragments arranged by size: 1069 696 83 PfoI T'CCnGG_A Cuts at: 0 1704 1848 Size: 1704 144 PpuMI rG'GwC_Cy Cuts at: 0 59 1166 1848 Size: 59 1107 682 Fragments arranged by size: 1107 682 59 PshAI GACnn'nnGTC Cuts at: 0 1295 1848 Size: 1295 553 PspOMI G'GGCC_C Cuts at: 0 545 1407 1633 1752 1848 Size: 545 862 226 119 96 Fragments arranged by size: 862 545 226 119 96 PstI C_TGCA'G Cuts at: 0 356 1304 1619 1848 Size: 356 948 315 229 Fragments arranged by size: 948 356 315 229 PvuII CAG'CTG Cuts at: 0 1053 1284 1565 1700 1848 Size: 1053 231 281 135 148 Fragments arranged by size: 1053 281 231 148 135 SacI G_AGCT'C Cuts at: 0 1685 1848 Size: 1685 163 SanDI GG'GwC_CC Cuts at: 0 1166 1848 Size: 1166 682 SapI GCTCTTCn'nnn_ Cuts at: 0 1690 1848 Size: 1690 158 SbfI CC_TGCA'GG Cuts at: 0 356 1848 Size: 356 1492 SexAI A'CCwGG_T Cuts at: 0 257 665 1257 1848 Size: 257 408 592 591 Fragments arranged by size: 592 591 408 257 SfcI C'TryA_G Cuts at: 0 352 1300 1431 1615 1848 Size: 352 948 131 184 233 Fragments arranged by size: 948 352 233 184 131 SmaI CCC'GGG Cuts at: 0 1807 1848 Size: 1807 41 SmlI C'TyrA_G Cuts at: 0 124 226 247 379 409 787 853 949 Size: 124 102 21 132 30 378 66 96 Cuts at: 949 1369 1848 Size: 420 479 Fragments arranged by size: 479 420 378 132 124 102 96 66 30 21 SphI G_CATG'C Cuts at: 0 186 981 1848 Size: 186 795 867 Fragments arranged by size: 867 795 186 StuI AGG'CCT Cuts at: 0 191 1848 Size: 191 1657 StyI C'CwwG_G Cuts at: 0 77 1655 1848 Size: 77 1578 193 Fragments arranged by size: 1578 193 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1090 1848 Size: 1090 758 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1595 1627 1848 Size: 1595 32 221 Fragments arranged by size: 1595 221 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1146 1281 1848 Size: 1146 135 567 Fragments arranged by size: 1146 567 135 XmaI C'CCGG_G Cuts at: 0 1805 1848 Size: 1805 43 XmnI GAAnn'nnTTC Cuts at: 0 310 1848 Size: 310 1538 Enzymes that do cut: AarI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BfrBI BglII BmtI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI EagI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI