Restriction map for
pFN21AE3259
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvaI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI TaqI TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1483 GGTACC G^GTACC
AlwNI 1841 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 468 CCTTAGG CC^T[ACGT]AGG
ApaI 1002 GGGCCC GGGCC^C
Asp718I 1483 GGTACC G^GTACC
AvaII 1664 GGTCC G^G[AT]CC
BbrPI 765 CACGTG CAC^GTG
BcgI 1353 CGAAAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 779 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1442 AGATCT A^GATCT
BsaAI 765 CACGTG [CT]AC^GT[GA]
BsmI 1368 GAATGC GAATGC(1/-1)
Bsp1407I 288 TGTACA T^GTACA
BspLU11I 76 ACATGT A^CATGT
BsrGI 288 TGTACA T^GTACA
BssSI 719 CACGAG CACGAG(-5/-1)
BstYI 1442 AGATCT [GA]^GATC[CT]
Bsu36I 468 CCTTAGG CC^T[ACGT]AGG
CfoI 399 GCGC GCG^C
DraI 108 TTTAAA TTT^AAA
DraIII 1190 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1829 CCGTGG C^C[GA][CT]GG
Eco81I 468 CCTTAGG CC^T[ACGT]AGG
EcoT14I 1110 CCAAGG C^C[AT][AT]GG
HhaI 399 GCGC GCG^C
HinP1I 399 GCGC G^CGC
HindIII 1182 AAGCTT A^AGCTT
KpnI 1483 GGTACC GGTAC^C
MflI 1442 AGATCT [GA]^GATC[CT]
NspI 76 ACATGT [GA]CATG^[CT]
PmaCI 765 CACGTG CAC^GTG
PmlI 765 CACGTG CAC^GTG
PvuII 1577 CAGCTG CAG^CTG
SacI 644 GAGCTC GAGCT^C
SapI 641 GAAGAGC GCTCTTC(1/4)
ScaI 605 AGTACT AGT^ACT
SmlI 1676 CTCAAG C^T[CT][GA]AG
SspBI 288 TGTACA T^GTACA
SspI 1025 AATATT AAT^ATT
StuI 1553 AGGCCT AGG^CCT
StyI 1110 CCAAGG C^C[AT][AT]GG
XcmI 402 CCAGTACACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1442 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 76 ACATGT A^C[GA][CT]GT
764 ACACGT
Alw44I 64 GTGCAC G^TGCAC
768 GTGCAC
AlwI 757 GATCC GGATC(4/5)
804 GGATC
ApaLI 64 GTGCAC G^TGCAC
768 GTGCAC
AspI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACACTGTC
BcnI 447 CCGGG CC^[GC]GG
1515 CCCGG
BpmI 662 CTGGAG CTGGAG(16/14)
925 CTGGAG
BsgI 828 CTGCAC GTGCAG(16/14)
1078 CTGCAC
DraII 1002 GGGCCCT [GA]G^G[ACGT]CC[CT]
1704 AGGCCCC
EcoNI 152 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
468 CCTTAGGGAGG
EcoO109I 1002 GGGCCCT [GA]G^G[ACGT]CC[CT]
1704 AGGCCCC
HapII 447 CCGG C^CGG
1516 CCGG
HincII 130 GTTGAC GT[CT]^[GA]AC
1528 GTTGAC
HindII 130 GTTGAC GT[CT]^[GA]AC
1528 GTTGAC
HpaII 447 CCGG C^CGG
1516 CCGG
MaeII 680 ACGT A^CGT
766 ACGT
MslI 339 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
906 CACAATAATG
MspA1I 1155 CAGCGG C[AC]G^C[GT]G
1577 CAGCTG
MspI 447 CCGG C^CGG
1516 CCGG
NciI 447 CCGGG CC^[GC]GG
1515 CCCGG
PflFI 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACACTGTC
SfaNI 1761 GCATC GCATC(5/9)
1817 GCATC
Tth111I 208 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanI BanII BbvI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI