(Circular) (Six-base) MAPSORT of: pFN21AE3259.seq Check: 4315 from: 1 to: 6556 pFN21AE3259 6556 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2786 3096 2786 Size: 310 6246 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3899 1937 Size: 1962 4594 Acc65I G'GTAC_C Cuts at: 3488 3877 3488 Size: 389 6167 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3120 3306 4619 6003 6271 1632 Size: 1488 186 1313 1384 268 1917 Fragments arranged by size: 1917 1488 1384 1313 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2081 2769 5455 6432 1203 Size: 878 688 2686 977 1327 Fragments arranged by size: 2686 1327 977 878 688 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 2348 1166 Size: 1182 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6556 AlwNI CAG_nnn'CTG Cuts at: 3851 4299 5871 3851 Size: 448 1572 4536 Fragments arranged by size: 4536 1572 448 ApaI G_GGCC'C Cuts at: 3011 3011 Size: 6556 ApaLI G'TGCA_C Cuts at: 2069 2773 4613 5769 2069 Size: 704 1840 1156 2856 Fragments arranged by size: 2856 1840 1156 704 ApoI r'AATT_y Cuts at: 1493 2109 2873 3689 3778 3865 4091 4243 6352 Size: 616 764 816 89 87 226 152 2109 Cuts at: 6352 1493 Size: 1697 Fragments arranged by size: 2109 1697 816 764 616 226 152 89 87 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 3293 3344 3488 3502 Size: 325 217 627 1406 100 51 144 14 Cuts at: 3502 3566 3877 5332 618 Size: 64 311 1455 1842 Fragments arranged by size: 1842 1455 1406 627 325 311 217 144 100 64 51 14 BanII G_rGCy'C Cuts at: 1391 2653 3011 3069 3875 1391 Size: 1262 358 58 806 4072 Fragments arranged by size: 4072 1262 806 358 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3347 3381 4747 4781 1903 Size: 34 1410 34 1366 34 3678 Fragments arranged by size: 3678 1410 1366 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 1548 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2790 5167 136 Size: 107 122 71 631 605 1118 2377 1525 Fragments arranged by size: 2377 1525 1118 631 605 122 107 71 BglII A'GATC_T Cuts at: 3447 6551 3447 Size: 3104 3452 BlpI GC'TnA_GC Cuts at: 3973 3973 Size: 6556 Bme1580I G_kGCm'C Cuts at: 1790 2073 2355 2777 3011 3296 3642 4617 5773 Size: 283 282 422 234 285 346 975 1156 Cuts at: 5773 1790 Size: 2573 Fragments arranged by size: 2573 1156 975 422 346 285 283 282 234 BmgBI CAC'GTC Cuts at: 1318 2686 1318 Size: 1368 5188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 4287 5245 Size: 416 53 83 399 24 932 1938 958 Cuts at: 5245 442 Size: 1753 Fragments arranged by size: 1938 1753 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 2951 5216 1239 Size: 1449 263 2265 2579 Fragments arranged by size: 2579 2265 1449 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3666 4681 5546 5844 3666 Size: 1015 865 298 4378 Fragments arranged by size: 4378 1015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5219 882 Size: 617 3720 2219 Fragments arranged by size: 3720 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2772 4347 493 Size: 711 1568 1575 2702 Fragments arranged by size: 2702 1575 1568 711 BsaBI GATnn'nnATC Cuts at: 6550 6550 Size: 6556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6331 Fragments arranged by size: 6331 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 2146 3137 3140 3152 3227 3230 3233 Size: 948 12 991 3 12 75 3 3 Cuts at: 3233 3254 3257 3260 3604 1186 Size: 21 3 3 344 4138 Fragments arranged by size: 4138 991 948 344 75 21 12 12 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3580 5759 6255 3580 Size: 2179 496 3881 Fragments arranged by size: 3881 2179 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2818 3068 1163 Size: 573 1082 250 4651 Fragments arranged by size: 4651 1082 573 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2653 2777 2992 4617 4702 5773 2073 Size: 580 124 215 1625 85 1071 2856 Fragments arranged by size: 2856 1625 1071 580 215 124 85 BsmI GAATG_Cn' Cuts at: 3379 4067 4160 3379 Size: 688 93 5775 Fragments arranged by size: 5775 688 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2355 2653 2777 2992 3011 3069 Size: 399 283 282 298 124 215 19 58 Cuts at: 3069 3296 3642 3875 4617 4702 5773 1391 Size: 227 346 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 298 283 282 233 227 215 124 85 58 19 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 4445 6175 4445 Size: 1730 4826 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2066 2786 2852 3096 3911 844 Size: 1222 720 66 244 815 3489 Fragments arranged by size: 3489 1222 815 720 244 66 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5051 5225 65 Size: 1183 3803 174 1396 Fragments arranged by size: 3803 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5200 5387 1700 Size: 110 3390 187 2869 Fragments arranged by size: 3390 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2293 96 Size: 2197 4359 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 2724 4616 5628 2724 Size: 1892 1012 3652 Fragments arranged by size: 3652 1892 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2799 3156 3374 794 Size: 2005 357 218 3976 Fragments arranged by size: 3976 2005 357 218 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6556 BstYI r'GATC_y Cuts at: 1972 3447 3886 4253 4638 4655 6107 6193 6205 Size: 1475 439 367 385 17 1452 86 12 Cuts at: 6205 6551 1972 Size: 346 1977 Fragments arranged by size: 1977 1475 1452 439 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2474 2474 Size: 6556 BtgI C'CryG_G Cuts at: 513 1065 3834 513 Size: 552 2769 3235 Fragments arranged by size: 3235 2769 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2114 487 Size: 683 56 888 4929 Fragments arranged by size: 4929 888 683 56 BtsI GCAGTG_nn' Cuts at: 2091 2197 3084 4072 4866 4886 2091 Size: 106 887 988 794 20 3761 Fragments arranged by size: 3761 988 887 794 106 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 2115 3860 2115 Size: 1745 4811 DraIII CAC_nnn'GTG Cuts at: 3200 3200 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4003 4892 6521 Size: 54 1425 50 275 18 2173 889 1629 Cuts at: 6521 8 Size: 43 Fragments arranged by size: 2173 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2641 3129 3225 3231 3321 3400 3549 3622 Size: 1288 488 96 6 90 79 149 73 Cuts at: 3622 3810 4486 1353 Size: 188 676 3423 Fragments arranged by size: 3423 1288 676 488 188 149 96 90 79 73 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 5143 5517 5663 387 Size: 1064 138 1116 2438 374 146 1280 Fragments arranged by size: 2438 1280 1116 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2651 2651 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 2951 3120 3306 4619 5216 6003 Size: 393 1056 263 169 186 1313 597 787 Cuts at: 6003 6271 1239 Size: 268 1524 Fragments arranged by size: 1524 1313 1056 787 597 393 268 263 186 169 EcoNI CCTnn'n_nnAGG Cuts at: 2161 2477 2161 Size: 316 6240 EcoO109I rG'GnC_Cy Cuts at: 3008 3710 3008 Size: 702 5854 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2801 2833 2974 Size: 32 135 32 557 32 640 32 141 Cuts at: 2974 3006 3462 3494 5197 5229 5271 5303 1373 Size: 32 456 32 1703 32 42 32 2626 Fragments arranged by size: 2626 1703 640 557 456 141 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 3535 3900 4146 669 Size: 988 281 199 1398 365 246 3079 Fragments arranged by size: 3079 1398 988 365 281 246 199 HindIII A'AGCT_T Cuts at: 748 3187 748 Size: 2439 4117 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3492 3881 3492 Size: 389 6167 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5670 5854 1305 Size: 4365 184 2007 Fragments arranged by size: 4365 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4734 Fragments arranged by size: 4734 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2915 4514 4873 5032 518 Size: 648 1182 567 1599 359 159 2042 Fragments arranged by size: 2042 1599 1182 648 567 359 159 MspA1I CmG'CkG Cuts at: 1606 3162 3584 3972 4649 5797 6042 1606 Size: 1556 422 388 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 677 422 388 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6004 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2085 3914 5459 1132 Size: 97 856 1829 1545 2229 Fragments arranged by size: 2229 1829 1545 856 97 PasI CC'CwG_GG Cuts at: 1348 2374 1348 Size: 1026 5530 PciI A'CATG_T Cuts at: 2081 5455 2081 Size: 3374 3182 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6556 PfoI T'CCnGG_A Cuts at: 3430 3430 Size: 6556 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PmlI CAC'GTG Cuts at: 2772 2772 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PspOMI G'GGCC_C Cuts at: 3007 3007 Size: 6556 PstI C_TGCA'G Cuts at: 830 2098 2783 3079 3093 3908 830 Size: 1268 685 296 14 815 3478 Fragments arranged by size: 3478 1268 815 685 296 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 3584 3584 Size: 6556 SacI G_AGCT'C Cuts at: 2653 2653 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SapI GCTCTTCn'nnn_ Cuts at: 2641 2641 Size: 6556 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 2612 4804 6516 2612 Size: 2192 1712 2652 Fragments arranged by size: 2652 2192 1712 SfcI C'TryA_G Cuts at: 826 1045 2094 2779 3075 3089 3904 5039 5720 Size: 219 1049 685 296 14 815 1135 681 Cuts at: 5720 5911 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1049 815 685 681 296 219 191 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3681 4660 5561 5823 820 Size: 197 926 1738 979 901 262 1553 Fragments arranged by size: 1738 1553 979 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 3032 4480 5 Size: 47 2980 1448 2081 Fragments arranged by size: 2980 2081 1448 47 StuI AGG'CCT Cuts at: 1517 3560 1517 Size: 2043 4513 StyI C'CwwG_G Cuts at: 513 1065 3115 3995 513 Size: 552 2050 880 3074 Fragments arranged by size: 3074 2050 880 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 4583 4600 4753 4938 5383 325 Size: 1255 1731 1272 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1272 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2293 2316 2409 Size: 274 80 33 51 1443 316 23 93 Cuts at: 2409 2610 4802 6514 96 Size: 201 2192 1712 138 Fragments arranged by size: 2192 1712 1443 316 274 201 138 93 80 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6516 6548 6516 Size: 32 6524 Tth111I GACn'n_nGTC Cuts at: 1316 2216 3476 1316 Size: 900 1260 4396 Fragments arranged by size: 4396 1260 900 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2414 2414 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 4685 4685 Size: 6556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI