(Linear) (Six-base) MAPSORT of: KIEE3259.seq Check: 9461 from: 1 to: 1851 KIEE3259.orf With 182 enzymes: SgfI * July 3, 2012 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 781 1091 1851 Size: 781 310 760 Fragments arranged by size: 781 760 310 Acc65I G'GTAC_C Cuts at: 0 1483 1851 Size: 1483 368 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1115 1301 1851 Size: 1115 186 550 Fragments arranged by size: 1115 550 186 AflIII A'CryG_T Cuts at: 0 76 764 1851 Size: 76 688 1087 Fragments arranged by size: 1087 688 76 AleI CACnn'nnGTG Cuts at: 0 343 1851 Size: 343 1508 AlwNI CAG_nnn'CTG Cuts at: 0 1846 1851 Size: 1846 5 ApaI G_GGCC'C Cuts at: 0 1006 1851 Size: 1006 845 ApaLI G'TGCA_C Cuts at: 0 64 768 1851 Size: 64 704 1083 Fragments arranged by size: 1083 704 64 ApoI r'AATT_y Cuts at: 0 104 868 1684 1773 1851 Size: 104 764 816 89 78 Fragments arranged by size: 816 764 104 89 78 BanI G'GyrC_C Cuts at: 0 1188 1288 1339 1483 1497 1561 1851 Size: 1188 100 51 144 14 64 290 Fragments arranged by size: 1188 290 144 100 64 51 14 BanII G_rGCy'C Cuts at: 0 648 1006 1064 1851 Size: 648 358 58 787 Fragments arranged by size: 787 648 358 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1342 1376 1851 Size: 1342 34 475 Fragments arranged by size: 1342 475 34 BglI GCCn_nnn'nGGC Cuts at: 0 785 1851 Size: 785 1066 BglII A'GATC_T Cuts at: 0 1442 1851 Size: 1442 409 Bme1580I G_kGCm'C Cuts at: 0 68 350 772 1006 1291 1637 1851 Size: 68 282 422 234 285 346 214 Fragments arranged by size: 422 346 285 282 234 214 68 BmgBI CAC'GTC Cuts at: 0 681 1851 Size: 681 1170 BmrI ACTGGGnnnn_n' Cuts at: 0 344 1851 Size: 344 1507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 946 1851 Size: 683 263 905 Fragments arranged by size: 905 683 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1661 1851 Size: 1661 190 BsaAI yAC'GTr Cuts at: 0 767 1851 Size: 767 1084 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 129 141 1132 1135 1147 1222 1225 1228 Size: 129 12 991 3 12 75 3 3 Cuts at: 1228 1249 1252 1255 1599 1851 Size: 21 3 3 344 252 Fragments arranged by size: 991 344 252 129 75 21 12 12 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1575 1851 Size: 1575 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 813 1063 1851 Size: 813 250 788 Fragments arranged by size: 813 788 250 BsiHKAI G_wGCw'C Cuts at: 0 68 648 772 987 1851 Size: 68 580 124 215 864 Fragments arranged by size: 864 580 215 124 68 BsmI GAATG_Cn' Cuts at: 0 1374 1851 Size: 1374 477 Bsp1286I G_dGCh'C Cuts at: 0 68 350 648 772 987 1006 1064 1291 Size: 68 282 298 124 215 19 58 227 Cuts at: 1291 1637 1851 Size: 346 214 Fragments arranged by size: 346 298 282 227 215 214 124 68 58 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 61 781 847 1091 1851 Size: 61 720 66 244 760 Fragments arranged by size: 760 720 244 66 61 BsrGI T'GTAC_A Cuts at: 0 288 1851 Size: 288 1563 BssSI C'ACGA_G Cuts at: 0 719 1851 Size: 719 1132 BstAPI GCAn_nnn'nTGC Cuts at: 0 794 1151 1369 1851 Size: 794 357 218 482 Fragments arranged by size: 794 482 357 218 BstYI r'GATC_y Cuts at: 0 1442 1851 Size: 1442 409 Bsu36I CC'TnA_GG Cuts at: 0 469 1851 Size: 469 1382 BtgI C'CryG_G Cuts at: 0 1829 1851 Size: 1829 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 109 1851 Size: 109 1742 BtsI GCAGTG_nn' Cuts at: 0 86 192 1079 1851 Size: 86 106 887 772 Fragments arranged by size: 887 772 106 86 DraI TTT'AAA Cuts at: 0 110 1851 Size: 110 1741 DraIII CAC_nnn'GTG Cuts at: 0 1195 1851 Size: 1195 656 EarI CTCTTCn'nnn_ Cuts at: 0 636 1124 1220 1226 1316 1395 1544 1617 Size: 636 488 96 6 90 79 149 73 Cuts at: 1617 1805 1851 Size: 188 46 Fragments arranged by size: 636 488 188 149 96 90 79 73 46 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 700 1851 Size: 700 1151 EcoICRI GAG'CTC Cuts at: 0 646 1851 Size: 646 1205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 946 1115 1301 1851 Size: 683 263 169 186 550 Fragments arranged by size: 683 550 263 186 169 EcoNI CCTnn'n_nnAGG Cuts at: 0 156 472 1851 Size: 156 316 1379 Fragments arranged by size: 1379 316 156 EcoO109I rG'GnC_Cy Cuts at: 0 1003 1705 1851 Size: 1003 702 146 Fragments arranged by size: 1003 702 146 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 796 828 969 1001 1457 1489 Size: 124 32 640 32 141 32 456 32 Cuts at: 1489 1851 Size: 362 Fragments arranged by size: 640 456 362 141 124 32 32 32 32 HincII GTy'rAC Cuts at: 0 132 1530 1851 Size: 132 1398 321 Fragments arranged by size: 1398 321 132 HindIII A'AGCT_T Cuts at: 0 1182 1851 Size: 1182 669 KpnI G_GTAC'C Cuts at: 0 1487 1851 Size: 1487 364 MslI CAynn'nnrTG Cuts at: 0 343 910 1851 Size: 343 567 941 Fragments arranged by size: 941 567 343 MspA1I CmG'CkG Cuts at: 0 1157 1579 1851 Size: 1157 422 272 Fragments arranged by size: 1157 422 272 NspI r_CATG'y Cuts at: 0 80 1851 Size: 80 1771 PasI CC'CwG_GG Cuts at: 0 369 1851 Size: 369 1482 PciI A'CATG_T Cuts at: 0 76 1851 Size: 76 1775 PfoI T'CCnGG_A Cuts at: 0 1425 1851 Size: 1425 426 PmlI CAC'GTG Cuts at: 0 767 1851 Size: 767 1084 PspOMI G'GGCC_C Cuts at: 0 1002 1851 Size: 1002 849 PstI C_TGCA'G Cuts at: 0 93 778 1074 1088 1851 Size: 93 685 296 14 763 Fragments arranged by size: 763 685 296 93 14 PvuII CAG'CTG Cuts at: 0 1579 1851 Size: 1579 272 SacI G_AGCT'C Cuts at: 0 648 1851 Size: 648 1203 SapI GCTCTTCn'nnn_ Cuts at: 0 636 1851 Size: 636 1215 ScaI AGT'ACT Cuts at: 0 607 1851 Size: 607 1244 SfcI C'TryA_G Cuts at: 0 89 774 1070 1084 1851 Size: 89 685 296 14 767 Fragments arranged by size: 767 685 296 89 14 SmlI C'TyrA_G Cuts at: 0 1676 1851 Size: 1676 175 SspI AAT'ATT Cuts at: 0 1027 1851 Size: 1027 824 StuI AGG'CCT Cuts at: 0 1555 1851 Size: 1555 296 StyI C'CwwG_G Cuts at: 0 1110 1851 Size: 1110 741 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1306 1851 Size: 1306 545 TatI w'GTAC_w Cuts at: 0 288 311 404 605 1851 Size: 288 23 93 201 1246 Fragments arranged by size: 1246 288 201 93 23 Tth111I GACn'n_nGTC Cuts at: 0 211 1471 1851 Size: 211 1260 380 Fragments arranged by size: 1260 380 211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 409 1851 Size: 409 1442 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI ApoI BanI BanII BcgI BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MslI MspA1I NspI PasI PciI PfoI PmlI PspOMI PstI PvuII SacI SapI ScaI SfcI SmlI SspI StuI StyI TaqII TatI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI DrdI EaeI EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI XbaI XhoI XmaI XmnI ZraI