################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanII BbrPI BbvCI BcgI BclI BglI BlnI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmBI BspDI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccIII 1442 TCCGGA T^CCGGA AflII 1728 CTTAAG C^TTAAG AflIII 420 ACATGT A^C[GA][CT]GT AgeI 6 ACCGGT A^CCGGT AlwNI 1684 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 811 CTCGGG C^[CT]CG[GA]G AvaII 1445 GGACC G^G[AT]CC BamHI 1120 GGATCC G^GATCC BbeI 59 GGCGCC GGCGC^C BbsI 1393 GAAGAC GAAGAC(2/6) BciVI 405 GTATCC GTATCC(6/5) BfrI 1728 CTTAAG C^TTAAG BlpI 1329 GCTCAGC GC^T[ACGT]AGC BmyI 1542 GTGCCC G[AGT]GC[ACT]^C BpmI 1519 CTGGAG CTGGAG(16/14) Bpu1102I 1329 GCTCAGC GC^T[ACGT]AGC BpuAI 1393 GAAGAC GAAGAC(2/6) BsaI 534 GAGACC GGTCTC(1/5) BseAI 1442 TCCGGA T^CCGGA BsgI 1029 CTGCAC GTGCAG(16/14) BsmFI 301 GGGAC GGGAC(10/14) BsoBI 811 CTCGGG C^[CT]CG[GA]G Bsp1286I 1542 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 417 TGTACA T^GTACA BspEI 1442 TCCGGA T^CCGGA BspHI 1385 TCATGA T^CATGA BspLU11I 420 ACATGT A^CATGT BspMI 1100 GCAGGT ACCTGC(4/8) BsrFI 6 ACCGGT [GA]^CCGG[CT] BsrGI 417 TGTACA T^GTACA BstAPI 1562 GCACCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 290 TTCGAA TT^CGAA CelII 1329 GCTCAGC GC^T[ACGT]AGC CfoI 60 GCGC GCG^C Cfr10I 6 ACCGGT [GA]^CCGG[CT] DraIII 1563 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 635 CTCTTC CTCTTC(1/4) EcoRI 458 GAATTC G^AATTC EcoT14I 1533 CCTTGG C^C[AT][AT]GG HaeII 59 GGCGCC [GA]GCGC^[CT] HgaI 348 GACGC GACGC(5/10) HhaI 60 GCGC GCG^C HinP1I 60 GCGC G^CGC HincII 21 GTTAAC GT[CT]^[GA]AC HindII 21 GTTAAC GT[CT]^[GA]AC HpaI 21 GTTAAC GTT^AAC KasI 59 GGCGCC G^GCGCC Ksp632I 635 CTCTTC CTCTTC(1/4) MroI 1442 TCCGGA T^CCGGA MslI 937 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 59 GGCGCC GG^CGCC NheI 1411 GCTAGC G^CTAGC NspV 290 TTCGAA TT^CGAA PinAI 6 ACCGGT A^CCGGT PstI 1552 CTGCAG CTGCA^G RcaI 1385 TCATGA T^CATGA SexAI 1675 ACCAGGT A^CC[AT]GGT SfoI 59 GGCGCC GGC^GCC SfuI 290 TTCGAA TT^CGAA SspBI 417 TGTACA T^GTACA StuI 1211 AGGCCT AGG^CCT StyI 1533 CCTTGG C^C[AT][AT]GG XbaI 426 TCTAGA T^CTAGA XcmI 164 CCAAAGAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 59 GGCGCC G[GA]^CG[CT]C 348 GACGCC BanI 59 GGCGCC G^G[CT][GA]CC 1541 GGTGCC BcnI 442 CCCGG CC^[GC]GG 1565 CCGGG BglII 1630 AGATCT A^GATCT 1755 AGATCT BsaHI 59 GGCGCC G[GA]^CG[CT]C 348 GACGCC BsaWI 6 ACCGGT [AT]^CCGG[AT] 1442 TCCGGA BsmAI 534 GAGAC GTCTC(1/5) 1023 GTCTC BsmI 549 GAATGC GAATGC(1/-1) 1818 GAATGC BsrDI 937 CATTGC GCAATG(2/0) 1263 CATTGC Cfr13I 820 GGGCC G^G[ACGT]CC 1445 GGACC HaeIII 821 GGCC GG^CC 1212 GGCC Hin1I 59 GGCGCC G[GA]^CG[CT]C 348 GACGCC HindIII 1250 AAGCTT A^AGCTT 1824 AAGCTT NciI 442 CCCGG CC^[GC]GG 1565 CCGGG NspI 420 ACATGT [GA]CATG^[CT] 1663 GCATGT PflMI 256 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 823 CCAAATAATGG Sau96I 820 GGGCC G^G[ACGT]CC 1445 GGACC SspI 644 AATATT AAT^ATT 1471 AATATT TaqI 291 TCGA T^CGA 1302 TCGA TthHB8I 291 TCGA T^CGA 1302 TCGA Van91I 256 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 823 CCAAATAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII