(Circular) (Six-base) MAPSORT of: pFN21AE4170.seq Check: 5403 from: 1 to: 6568 pFN21AE4170 6568 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2529 3134 3347 4631 6015 6283 1632 Size: 897 605 213 1284 1384 268 1917 Fragments arranged by size: 1917 1384 1284 897 605 268 213 AflII C'TTAA_G Cuts at: 820 1017 3733 820 Size: 197 2716 3655 Fragments arranged by size: 3655 2716 197 AflIII A'CryG_T Cuts at: 1203 2425 5467 6444 1203 Size: 1222 3042 977 1327 Fragments arranged by size: 3042 1327 1222 977 AgeI A'CCGG_T Cuts at: 2011 5399 2011 Size: 3388 3180 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2938 1184 Size: 1754 4814 AlwNI CAG_nnn'CTG Cuts at: 3694 4311 5883 3694 Size: 617 1572 4379 Fragments arranged by size: 4379 1572 617 ApaLI G'TGCA_C Cuts at: 4625 5781 4625 Size: 1156 5412 ApoI r'AATT_y Cuts at: 1493 2233 2463 2599 3017 3877 4103 4255 6364 Size: 740 230 136 418 860 226 152 2109 Cuts at: 6364 1493 Size: 1697 Fragments arranged by size: 2109 1697 860 740 418 230 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2816 3893 1870 Size: 73 873 1077 4545 Fragments arranged by size: 4545 1077 873 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2393 2426 2393 Size: 33 6535 BamHI G'GATC_C Cuts at: 3125 3898 4265 3125 Size: 773 367 5428 Fragments arranged by size: 5428 773 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 3546 3889 5344 618 Size: 325 217 627 277 1482 343 1455 1842 Fragments arranged by size: 1842 1482 1455 627 343 325 277 217 BanII G_rGCy'C Cuts at: 1391 3887 1391 Size: 2496 4072 BbeI G_GCGC'C Cuts at: 2068 2068 Size: 6568 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3405 928 Size: 971 1506 4091 Fragments arranged by size: 4091 1506 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2421 4460 5670 2421 Size: 2039 1210 3319 Fragments arranged by size: 3319 2039 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3635 3760 6563 3635 Size: 125 2803 3640 Fragments arranged by size: 3640 2803 125 BlpI GC'TnA_GC Cuts at: 3335 3985 3335 Size: 650 5918 Bme1580I G_kGCm'C Cuts at: 1790 3551 4629 5785 1790 Size: 1761 1078 1156 2573 Fragments arranged by size: 2573 1761 1156 1078 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2998 4299 5257 Size: 416 53 83 399 24 1581 1301 958 Cuts at: 5257 442 Size: 1753 Fragments arranged by size: 1753 1581 1301 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3420 1056 Size: 2364 4204 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3879 3879 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3545 5228 1239 Size: 2306 1683 2579 Fragments arranged by size: 2579 2306 1683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2696 3237 4693 5558 5856 2696 Size: 541 1456 865 298 3408 Fragments arranged by size: 3408 1456 865 541 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2533 5231 882 Size: 617 1034 2698 2219 Fragments arranged by size: 2698 2219 1034 617 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 2354 4375 4757 Size: 53 83 186 936 532 289 2021 382 Cuts at: 4757 5392 275 Size: 635 1451 Fragments arranged by size: 2021 1451 936 635 532 382 289 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 3447 5001 5399 5673 5820 6231 2011 Size: 1436 1554 398 274 147 411 2348 Fragments arranged by size: 2348 1554 1436 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3582 3612 1178 Size: 30 165 30 2179 30 4134 Fragments arranged by size: 4134 2179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 3220 3542 1186 Size: 917 1117 322 4212 Fragments arranged by size: 4212 1117 917 322 BseYI C'CCAG_C Cuts at: 5771 6267 5771 Size: 496 6072 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3019 1163 Size: 573 1283 4712 Fragments arranged by size: 4712 1283 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4018 4779 5807 1540 Size: 275 186 2017 761 1028 2301 Fragments arranged by size: 2301 2017 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4629 4714 5785 4629 Size: 85 1071 5412 Fragments arranged by size: 5412 1071 85 BsmI GAATG_Cn' Cuts at: 2560 3829 4079 4172 2560 Size: 1269 250 93 4956 Fragments arranged by size: 4956 1269 250 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3551 3887 4629 4714 5785 1391 Size: 399 1761 336 742 85 1071 2174 Fragments arranged by size: 2174 1761 1071 742 399 336 85 BspEI T'CCGG_A Cuts at: 3447 6231 3447 Size: 2784 3784 BspHI T'CATG_A Cuts at: 3390 4457 6187 3390 Size: 1067 1730 3771 Fragments arranged by size: 3771 1730 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3096 3923 844 Size: 2252 827 3489 Fragments arranged by size: 3489 2252 827 BsrBI CCG'CTC Cuts at: 1394 1823 4455 1394 Size: 429 2632 3507 Fragments arranged by size: 3507 2632 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2941 3267 5063 5237 65 Size: 1183 1693 326 1796 174 1396 Fragments arranged by size: 1796 1693 1396 1183 326 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 5212 5399 1700 Size: 110 201 3201 187 2869 Fragments arranged by size: 3201 2869 201 187 110 BsrGI T'GTAC_A Cuts at: 96 2422 96 Size: 2326 4242 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 3573 794 Size: 2779 3789 BstBI TT'CG_AA Cuts at: 2296 5372 2296 Size: 3076 3492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstYI r'GATC_y Cuts at: 1972 3125 3457 3635 3760 3898 4265 4650 4667 Size: 1153 332 178 125 138 367 385 17 Cuts at: 4667 6119 6205 6217 6563 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1153 385 367 346 332 178 138 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6016 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 2812 3329 4084 4878 4898 2812 Size: 517 755 794 20 4482 Fragments arranged by size: 4482 794 755 517 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DraIII CAC_nnn'GTG Cuts at: 3573 3573 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4015 4904 6533 Size: 54 1425 50 275 18 2185 889 1629 Cuts at: 6533 8 Size: 43 Fragments arranged by size: 2185 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 4498 1353 Size: 1293 1852 3423 Fragments arranged by size: 3423 1852 1293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2529 3134 3347 3545 4631 5228 6015 Size: 393 897 605 213 198 1086 597 787 Cuts at: 6015 6283 1239 Size: 268 1524 Fragments arranged by size: 1524 1086 897 787 605 597 393 268 213 198 EcoRI G'AATT_C Cuts at: 2463 3877 4255 2463 Size: 1414 378 4776 Fragments arranged by size: 4776 1414 378 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 2068 5715 1678 Size: 390 3647 2531 Fragments arranged by size: 3647 2531 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2125 2157 2866 2898 3069 Size: 32 135 32 553 32 709 32 171 Cuts at: 3069 3101 3285 3317 3442 3474 5209 5241 5283 Size: 32 184 32 125 32 1735 32 42 Cuts at: 5283 5315 1373 Size: 32 2626 Fragments arranged by size: 2626 1735 709 553 184 171 135 125 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 3912 4158 669 Size: 988 281 90 1884 246 3079 Fragments arranged by size: 3079 1884 988 281 246 90 HindIII A'AGCT_T Cuts at: 748 3255 3829 748 Size: 2507 574 3487 Fragments arranged by size: 3487 2507 574 HpaI GTT'AAC Cuts at: 2028 4158 2028 Size: 2130 4438 KasI G'GCGC_C Cuts at: 2064 2064 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5682 5866 1305 Size: 4377 184 2007 Fragments arranged by size: 4377 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4746 Fragments arranged by size: 4746 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2946 4526 4885 5044 518 Size: 648 1780 1580 359 159 2042 Fragments arranged by size: 2042 1780 1580 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2288 2420 2507 2872 3694 3984 4661 5809 Size: 682 132 87 365 822 290 677 1148 Cuts at: 5809 6054 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 822 682 677 365 290 245 132 87 NarI GG'CG_CC Cuts at: 2065 2065 Size: 6568 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6016 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 3416 1052 Size: 2364 4204 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 2429 3672 3926 5471 1132 Size: 97 1200 1243 254 1545 2229 Fragments arranged by size: 2229 1545 1243 1200 254 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6568 PciI A'CATG_T Cuts at: 2425 5467 2425 Size: 3042 3526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2267 2834 1108 Size: 1159 567 4842 Fragments arranged by size: 4842 1159 567 PfoI T'CCnGG_A Cuts at: 2446 3744 2446 Size: 1298 5270 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PstI C_TGCA'G Cuts at: 830 3561 3920 830 Size: 2731 359 3478 Fragments arranged by size: 3478 2731 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2288 2420 2507 2872 3694 2288 Size: 132 87 365 822 5162 Fragments arranged by size: 5162 822 365 132 87 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SexAI A'CCwGG_T Cuts at: 3680 3680 Size: 6568 SfcI C'TryA_G Cuts at: 826 1045 2115 2415 3023 3212 3557 3916 5051 Size: 219 1070 300 608 189 345 359 1135 Cuts at: 5051 5732 5923 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1070 681 608 359 345 300 219 191 189 SfoI GGC'GCC Cuts at: 2066 2066 Size: 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2711 3252 3733 4672 5573 5835 Size: 197 926 768 541 481 939 901 262 Cuts at: 5835 820 Size: 1553 Fragments arranged by size: 1553 939 926 901 768 541 481 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 2651 3478 4492 5 Size: 47 2599 827 1014 2081 Fragments arranged by size: 2599 2081 1014 827 47 StuI AGG'CCT Cuts at: 1517 3218 1517 Size: 1701 4867 StyI C'CwwG_G Cuts at: 513 1065 3538 4007 513 Size: 552 2473 469 3074 Fragments arranged by size: 3074 2473 552 469 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2120 3501 4595 4612 4765 4950 5395 Size: 1255 540 1381 1094 17 153 185 445 Cuts at: 5395 325 Size: 1498 Fragments arranged by size: 1498 1381 1255 1094 540 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2422 4814 6526 Size: 274 80 33 51 1443 445 2392 1712 Cuts at: 6526 96 Size: 138 Fragments arranged by size: 2392 1712 1443 445 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3582 3614 6528 6560 3582 Size: 32 2914 32 3590 Fragments arranged by size: 3590 2914 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 2431 3904 2431 Size: 1473 5095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2176 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 4697 4697 Size: 6568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI