(Linear) (Six-base) MAPSORT of: KIEE4170.seq Check: 2919 from: 1 to: 1863 KIEE4170.orf With 182 enzymes: SgfI * July 3, 2012 18:43 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1129 1342 1863 Size: 524 605 213 521 Fragments arranged by size: 605 524 521 213 AflII C'TTAA_G Cuts at: 0 1728 1863 Size: 1728 135 AflIII A'CryG_T Cuts at: 0 420 1863 Size: 420 1443 AgeI A'CCGG_T Cuts at: 0 6 1863 Size: 6 1857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 933 1863 Size: 933 930 AlwNI CAG_nnn'CTG Cuts at: 0 1689 1863 Size: 1689 174 ApoI r'AATT_y Cuts at: 0 228 458 594 1012 1863 Size: 228 230 136 418 851 Fragments arranged by size: 851 418 230 228 136 AvaI C'yCGr_G Cuts at: 0 811 1863 Size: 811 1052 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 388 421 1863 Size: 388 33 1442 Fragments arranged by size: 1442 388 33 BamHI G'GATC_C Cuts at: 0 1120 1863 Size: 1120 743 BanI G'GyrC_C Cuts at: 0 59 1541 1863 Size: 59 1482 322 Fragments arranged by size: 1482 322 59 BbeI G_GCGC'C Cuts at: 0 63 1863 Size: 63 1800 BbsI GAAGACnn'nnnn_ Cuts at: 0 1400 1863 Size: 1400 463 BciVI GTATCCnnnnn_n' Cuts at: 0 416 1863 Size: 416 1447 BglII A'GATC_T Cuts at: 0 1630 1755 1863 Size: 1630 125 108 Fragments arranged by size: 1630 125 108 BlpI GC'TnA_GC Cuts at: 0 1330 1863 Size: 1330 533 Bme1580I G_kGCm'C Cuts at: 0 1546 1863 Size: 1546 317 BmrI ACTGGGnnnn_n' Cuts at: 0 993 1863 Size: 993 870 BmtI G_CTAG'C Cuts at: 0 1415 1863 Size: 1415 448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1540 1863 Size: 1540 323 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 1232 1863 Size: 691 541 631 Fragments arranged by size: 691 631 541 BsaI GGTCTCn'nnnn_ Cuts at: 0 528 1863 Size: 528 1335 BsaHI Gr'CG_yC Cuts at: 0 60 349 1863 Size: 60 289 1514 Fragments arranged by size: 1514 289 60 BsaWI w'CCGG_w Cuts at: 0 6 1442 1863 Size: 6 1436 421 Fragments arranged by size: 1436 421 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1577 1607 1863 Size: 1577 30 256 Fragments arranged by size: 1577 256 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 98 1215 1537 1863 Size: 98 1117 322 326 Fragments arranged by size: 1117 326 322 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1014 1863 Size: 1014 849 BsmI GAATG_Cn' Cuts at: 0 555 1824 1863 Size: 555 1269 39 Fragments arranged by size: 1269 555 39 Bsp1286I G_dGCh'C Cuts at: 0 1546 1863 Size: 1546 317 BspEI T'CCGG_A Cuts at: 0 1442 1863 Size: 1442 421 BspHI T'CATG_A Cuts at: 0 1385 1863 Size: 1385 478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1091 1863 Size: 1091 772 BsrDI GCAATG_nn' Cuts at: 0 936 1262 1863 Size: 936 326 601 Fragments arranged by size: 936 601 326 BsrFI r'CCGG_y Cuts at: 0 6 1863 Size: 6 1857 BsrGI T'GTAC_A Cuts at: 0 417 1863 Size: 417 1446 BstAPI GCAn_nnn'nTGC Cuts at: 0 1568 1863 Size: 1568 295 BstBI TT'CG_AA Cuts at: 0 291 1863 Size: 291 1572 BstYI r'GATC_y Cuts at: 0 1120 1452 1630 1755 1863 Size: 1120 332 178 125 108 Fragments arranged by size: 1120 332 178 125 108 BtsI GCAGTG_nn' Cuts at: 0 807 1324 1863 Size: 807 517 539 Fragments arranged by size: 807 539 517 DraIII CAC_nnn'GTG Cuts at: 0 1568 1863 Size: 1568 295 EarI CTCTTCn'nnn_ Cuts at: 0 641 1863 Size: 641 1222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1129 1342 1540 1863 Size: 524 605 213 198 323 Fragments arranged by size: 605 524 323 213 198 EcoRI G'AATT_C Cuts at: 0 458 1863 Size: 458 1405 HaeII r_GCGC'y Cuts at: 0 63 1863 Size: 63 1800 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 120 152 861 893 1064 1096 1280 1312 Size: 120 32 709 32 171 32 184 32 Cuts at: 1312 1437 1469 1863 Size: 125 32 394 Fragments arranged by size: 709 394 184 171 125 120 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 23 1863 Size: 23 1840 HindIII A'AGCT_T Cuts at: 0 1250 1824 1863 Size: 1250 574 39 Fragments arranged by size: 1250 574 39 HpaI GTT'AAC Cuts at: 0 23 1863 Size: 23 1840 KasI G'GCGC_C Cuts at: 0 59 1863 Size: 59 1804 MslI CAynn'nnrTG Cuts at: 0 941 1863 Size: 941 922 MspA1I CmG'CkG Cuts at: 0 283 415 502 867 1689 1863 Size: 283 132 87 365 822 174 Fragments arranged by size: 822 365 283 174 132 87 NarI GG'CG_CC Cuts at: 0 60 1863 Size: 60 1803 NheI G'CTAG_C Cuts at: 0 1411 1863 Size: 1411 452 NspI r_CATG'y Cuts at: 0 424 1667 1863 Size: 424 1243 196 Fragments arranged by size: 1243 424 196 PciI A'CATG_T Cuts at: 0 420 1863 Size: 420 1443 PflMI CCAn_nnn'nTGG Cuts at: 0 262 829 1863 Size: 262 567 1034 Fragments arranged by size: 1034 567 262 PfoI T'CCnGG_A Cuts at: 0 441 1739 1863 Size: 441 1298 124 Fragments arranged by size: 1298 441 124 PstI C_TGCA'G Cuts at: 0 1556 1863 Size: 1556 307 PvuII CAG'CTG Cuts at: 0 283 415 502 867 1689 1863 Size: 283 132 87 365 822 174 Fragments arranged by size: 822 365 283 174 132 87 SexAI A'CCwGG_T Cuts at: 0 1675 1863 Size: 1675 188 SfcI C'TryA_G Cuts at: 0 110 410 1018 1207 1552 1863 Size: 110 300 608 189 345 311 Fragments arranged by size: 608 345 311 300 189 110 SfoI GGC'GCC Cuts at: 0 61 1863 Size: 61 1802 SmlI C'TyrA_G Cuts at: 0 706 1247 1728 1863 Size: 706 541 481 135 Fragments arranged by size: 706 541 481 135 SspI AAT'ATT Cuts at: 0 646 1473 1863 Size: 646 827 390 Fragments arranged by size: 827 646 390 StuI AGG'CCT Cuts at: 0 1213 1863 Size: 1213 650 StyI C'CwwG_G Cuts at: 0 1533 1863 Size: 1533 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 115 1496 1863 Size: 115 1381 367 Fragments arranged by size: 1381 367 115 TatI w'GTAC_w Cuts at: 0 417 1863 Size: 417 1446 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1577 1609 1863 Size: 1577 32 254 Fragments arranged by size: 1577 254 32 XbaI T'CTAG_A Cuts at: 0 426 1863 Size: 426 1437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 171 1863 Size: 171 1692 Enzymes that do cut: AcuI AflII AflIII AgeI AlfI AlwNI ApoI AvaI BaeI BamHI BanI BbeI BbsI BciVI BglII BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsgI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstYI BtsI DraIII EarI Eco57MI EcoRI HaeII Hin4I HincII HindIII HpaI KasI MslI MspA1I NarI NheI NspI PciI PflMI PfoI PstI PvuII SexAI SfcI SfoI SmlI SspI StuI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BanII BbvCI BcgI BclI BfrBI BglI BmgBI BplI Bpu10I BsaAI BsaBI BseYI BsiEI BsiHKAI BsiWI BsmBI BsrBI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XhoI XmaI XmnI ZraI