################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AcsI AflII AflIII AgeI AocI ApoI AseI AsnI Asp700I Asp718I AvrII BalI BbrPI BbsI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI BstBI BstEII BstPI Bsu36I CelII ClaI DraI DrdI EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MunI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AclI 976 AACGTT AA^CGTT AhdI 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 447 GTGCAC G^TGCAC AlwNI 166 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 153 AGCGCT AGC^GCT ApaLI 447 GTGCAC G^TGCAC AscI 357 GGCGCGCC GG^CGCGCC AspEI 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 447 GTGCAC G[AT]GC[AT]^C AviII 752 TGCGCA TGC^GCA BamHI 1410 GGATCC G^GATCC BbvCI 156 GCTGAGG CCTCAGC(-5/-2) BciVI 139 GTATCC GTATCC(6/5) BglI 27 GCCGTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1263 CACGTA [CT]AC^GT[GA] BsiHKAI 447 GTGCAC G[AT]GC[AT]^C BsmBI 889 CGTCTC CGTCTC(1/5) BsrDI 1327 GCAATG GCAATG(2/0) Bst1107I 1075 GTATAC GTA^TAC BstYI 1410 GGATCC [GA]^GATC[CT] BstZ17I 1075 GTATAC GTA^TAC CpoI 416 CGGTCCG CG^G[AT]CCG DraIII 1559 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1781 CCATGG C^C[GA][CT]GG Eam1105I 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 153 AGCGCT AGC^GCT FspI 752 TGCGCA TGC^GCA MflI 1410 GGATCC [GA]^GATC[CT] MslI 1899 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1781 CCATGG C^CATGG NdeI 1645 CATATG CA^TATG PflMI 777 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1826 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 976 AACGTT AA^CGTT RsrII 416 CGGTCCG CG^G[AT]CCG SalI 207 GTCGAC G^TCGAC SgrAI 52 CGCCGGCG C[GA]^CCGG[CT]G SmlI 99 CTTGAG C^T[CT][GA]AG Tsp509I 1581 AATT ^AATT Van91I 777 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1301 CCATACTGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1410 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 207 GTCGAC GT^[AC][GT]AC 1075 GTATAC AfaI 867 GTAC GT^AC 1266 GTAC AlwI 1411 GATCC GGATC(4/5) 1410 GGATC AspI 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 495 GACGGCGTC BbeI 50 GGCGCC GGCGC^C 659 GGCGCC BfaI 176 CTAG C^TAG 1912 CTAG BsaI 430 GAGACC GGTCTC(1/5) 817 GAGACC BseRI 892 CTCCTC GAGGAG(10/8) 760 GAGGAG BsmI 1222 GAATGC GAATGC(1/-1) 1395 GCATTC BspMI 1829 ACCTGC ACCTGC(4/8) 510 GCAGGT BsrBI 791 CCGCTC CCGCTC(-3/-3) 602 GAGCGG BstAPI 165 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1161 GCAGACCGTGC BstXI 1579 CCAATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1745 CCAAGTGCTTGG DpnII 646 GATC ^GATC 1411 GATC EcoNI 393 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 864 CCTGTACCAGG EcoT14I 1781 CCATGG C^C[AT][AT]GG 1862 CCTTGG HincII 207 GTCGAC GT[CT]^[GA]AC 1623 GTTGAC HindII 207 GTCGAC GT[CT]^[GA]AC 1623 GTTGAC KasI 50 GGCGCC G^GCGCC 659 GGCGCC MaeI 176 CTAG C^TAG 1912 CTAG MaeIII 1286 GTGAC ^GT[ACGT]AC 1838 GTGAC MboI 646 GATC ^GATC 1411 GATC NarI 50 GGCGCC GG^CGCC 659 GGCGCC NdeII 646 GATC ^GATC 1411 GATC PflFI 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 495 GACGGCGTC RsaI 867 GTAC GT^AC 1266 GTAC Sau3AI 646 GATC ^GATC 1411 GATC SfoI 50 GGCGCC GGC^GCC 659 GGCGCC StuI 320 AGGCCT AGG^CCT 1034 AGGCCT StyI 1781 CCATGG C^C[AT][AT]GG 1862 CCTTGG Tsp45I 1286 GTGAC ^GT[GC]AC 1838 GTGAC Tth111I 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 495 GACGGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI PstI PvuII Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI TspRI TthHB8I