(Circular) (Six-base) MAPSORT of: pFN21AE3534.seq Check: 7023 from: 1 to: 6628 pFN21AE3534 6628 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2506 2506 Size: 6628 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2213 3081 3971 1937 Size: 276 868 890 4594 Fragments arranged by size: 4594 890 868 276 Acc65I G'GTAC_C Cuts at: 3949 3949 Size: 6628 AclI AA'CG_TT Cuts at: 1563 2982 4755 5128 1563 Size: 1419 1773 373 3063 Fragments arranged by size: 3063 1773 1419 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3118 3641 4691 6075 6343 1632 Size: 1486 523 1050 1384 268 1917 Fragments arranged by size: 1917 1486 1384 1050 523 268 AfeI AGC'GCT Cuts at: 2160 2160 Size: 6628 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 5527 6504 1203 Size: 4324 977 1327 Fragments arranged by size: 4324 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 3862 5357 3862 Size: 1495 5133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3030 3798 1184 Size: 1846 768 4014 Fragments arranged by size: 4014 1846 768 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 6596 AlwNI CAG_nnn'CTG Cuts at: 2176 4371 5943 2176 Size: 2195 1572 2861 Fragments arranged by size: 2861 2195 1572 ApaI G_GGCC'C Cuts at: 2310 2494 3397 3625 2310 Size: 184 903 228 5313 Fragments arranged by size: 5313 903 228 184 ApaLI G'TGCA_C Cuts at: 2452 4685 5841 2452 Size: 2233 1156 3239 Fragments arranged by size: 3239 2233 1156 ApoI r'AATT_y Cuts at: 1493 3937 4163 4315 6424 1493 Size: 2444 226 152 2109 1697 Fragments arranged by size: 2444 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2363 2363 Size: 6628 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 3090 3204 3369 3953 1870 Size: 73 1147 114 165 584 4545 Fragments arranged by size: 4545 1147 584 165 114 73 BamHI G'GATC_C Cuts at: 3415 3958 4325 3415 Size: 543 367 5718 Fragments arranged by size: 5718 543 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2055 2371 2407 2664 3949 Size: 325 217 627 268 316 36 257 1285 Cuts at: 3949 5404 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1285 627 325 316 268 257 217 36 BanII G_rGCy'C Cuts at: 1391 2016 2310 2494 3097 3397 3625 3947 1391 Size: 625 294 184 603 300 228 322 4072 Fragments arranged by size: 4072 625 603 322 300 294 228 184 BbeI G_GCGC'C Cuts at: 2059 2668 2059 Size: 609 6019 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5657 BbvCI CC'TCA_GC Cuts at: 2162 2162 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2155 4520 5730 2155 Size: 2365 1210 3053 Fragments arranged by size: 3053 2365 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2038 5239 136 Size: 107 122 71 631 605 366 3201 1525 Fragments arranged by size: 3201 1525 631 605 366 122 107 71 BglII A'GATC_T Cuts at: 6623 6623 Size: 6628 BlpI GC'TnA_GC Cuts at: 4045 4045 Size: 6628 Bme1580I G_kGCm'C Cuts at: 1790 2310 2376 2412 2456 2494 3397 3565 3625 Size: 520 66 36 44 38 903 168 60 Cuts at: 3625 4689 5845 1790 Size: 1064 1156 2573 Fragments arranged by size: 2573 1156 1064 903 520 168 66 60 44 38 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3619 4359 5317 Size: 416 53 83 399 24 2202 740 958 Cuts at: 5317 442 Size: 1753 Fragments arranged by size: 2202 1753 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 2342 3204 3621 5288 1239 Size: 761 342 862 417 1667 2579 Fragments arranged by size: 2579 1667 862 761 417 342 Bpu10I CC'TnA_GC Cuts at: 2162 2162 Size: 6628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2125 4753 5618 5916 2125 Size: 2628 865 298 2837 Fragments arranged by size: 2837 2628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 2816 5291 882 Size: 617 930 387 2475 2219 Fragments arranged by size: 2475 2219 930 617 387 BsaAI yAC'GTr Cuts at: 493 1204 3270 4419 493 Size: 711 2066 1149 2702 Fragments arranged by size: 2702 2066 1149 711 BsaBI GATnn'nnATC Cuts at: 6622 6622 Size: 6628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2056 2504 2665 2793 Size: 53 83 186 936 523 448 161 128 Cuts at: 2793 4435 4817 5452 275 Size: 1642 382 635 1451 Fragments arranged by size: 1642 1451 936 635 523 448 382 186 161 128 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 3594 3624 1178 Size: 30 165 30 1356 30 805 30 4182 Fragments arranged by size: 4182 1356 805 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 2888 1186 Size: 1594 108 4926 Fragments arranged by size: 4926 1594 108 BseYI C'CCAG_C Cuts at: 2070 2591 3396 3591 3771 5831 6327 2070 Size: 521 805 195 180 2060 496 2371 Fragments arranged by size: 2371 2060 805 521 496 195 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 2792 3236 3344 1163 Size: 573 310 746 444 108 4447 Fragments arranged by size: 4447 746 573 444 310 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2051 2220 2265 2475 2842 4078 Size: 275 186 50 169 45 210 367 1236 Cuts at: 4078 4839 5867 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1236 1028 761 367 275 210 186 169 50 45 BsiHKAI G_wGCw'C Cuts at: 2456 4689 4774 5845 2456 Size: 2233 85 1071 3239 Fragments arranged by size: 3239 2233 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3400 4139 4232 3233 Size: 167 739 93 5629 Fragments arranged by size: 5629 739 167 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2900 2900 Size: 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2310 2376 2412 2456 2494 3097 Size: 399 226 294 66 36 44 38 603 Cuts at: 3097 3397 3565 3625 3947 4689 4774 5845 1391 Size: 300 168 60 322 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 322 300 294 226 168 85 66 60 44 38 36 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 4517 6247 4517 Size: 1730 4898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2506 3843 3983 844 Size: 1662 1337 140 3489 Fragments arranged by size: 3489 1662 1337 140 BsrBI CCG'CTC Cuts at: 1394 1823 2609 2798 4515 1394 Size: 429 786 189 1717 3507 Fragments arranged by size: 3507 1717 786 429 189 BsrDI GCAATG_nn' Cuts at: 65 1248 3339 5123 5297 65 Size: 1183 2091 1784 174 1396 Fragments arranged by size: 2091 1784 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 2419 2539 2598 2661 2879 3157 Size: 110 248 361 120 59 63 218 278 Cuts at: 3157 5272 5459 1700 Size: 2115 187 2869 Fragments arranged by size: 2869 2115 361 278 248 218 187 120 110 63 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6628 BssHII G'CGCG_C Cuts at: 1925 2339 2363 2393 2844 1925 Size: 414 24 30 451 5709 Fragments arranged by size: 5709 451 414 30 24 BssSI C'ACGA_G Cuts at: 4688 5700 4688 Size: 1012 5616 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 3172 794 Size: 1382 996 4250 Fragments arranged by size: 4250 1382 996 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstXI CCAn_nnnn'nTGG Cuts at: 3591 3757 3591 Size: 166 6462 BstYI r'GATC_y Cuts at: 1972 3415 3958 4325 4710 4727 6179 6265 6277 Size: 1443 543 367 385 17 1452 86 12 Cuts at: 6277 6623 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1443 543 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3082 3082 Size: 6628 BtgI C'CryG_G Cuts at: 513 1065 2004 3786 513 Size: 552 939 1782 3355 Fragments arranged by size: 3355 1782 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2617 487 Size: 683 56 1391 4498 Fragments arranged by size: 4498 1391 683 56 BtsI GCAGTG_nn' Cuts at: 3327 4144 4938 4958 3327 Size: 817 794 20 4997 Fragments arranged by size: 4997 817 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DraIII CAC_nnn'GTG Cuts at: 3569 3569 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5635 809 Size: 507 4319 1802 Fragments arranged by size: 4319 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2217 2262 Size: 54 1425 50 275 18 218 169 45 Cuts at: 2262 2296 2472 2571 2779 2839 3495 4075 4964 Size: 34 176 99 208 60 656 580 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 656 580 275 218 208 176 169 99 60 54 50 45 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2048 2217 2262 2472 2839 4075 1537 Size: 275 236 169 45 210 367 1236 4090 Fragments arranged by size: 4090 1236 367 275 236 210 169 45 EarI CTCTTCn'nnn_ Cuts at: 1353 4558 1353 Size: 3205 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5215 5589 5735 387 Size: 1064 138 3626 374 146 1280 Fragments arranged by size: 3626 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2342 3118 3204 3621 3641 4691 Size: 393 368 342 776 86 417 20 1050 Cuts at: 4691 5288 6075 6343 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1050 787 776 597 417 393 368 342 268 86 20 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2873 2402 Size: 471 6157 EcoO109I rG'GnC_Cy Cuts at: 2306 2490 2491 3393 3621 3832 2306 Size: 184 1 902 228 211 5102 Fragments arranged by size: 5102 902 228 211 184 1 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3447 3447 Size: 6628 FspI TGC'GCA Cuts at: 2759 5134 2759 Size: 2375 4253 HaeII r_GCGC'y Cuts at: 1678 2059 2162 2668 5775 1678 Size: 381 103 506 3107 2531 Fragments arranged by size: 3107 2531 506 381 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3848 3880 5269 5301 5343 Size: 32 135 32 2276 32 1389 32 42 Cuts at: 5343 5375 1373 Size: 32 2626 Fragments arranged by size: 2626 2276 1389 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2214 3630 3972 4218 669 Size: 988 281 276 1416 342 246 3079 Fragments arranged by size: 3079 1416 988 342 281 276 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KasI G'GCGC_C Cuts at: 2055 2664 2055 Size: 609 6019 KpnI G_GTAC'C Cuts at: 3953 3953 Size: 6628 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3003 5742 5926 1305 Size: 1698 2739 184 2007 Fragments arranged by size: 2739 2007 1698 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4806 Fragments arranged by size: 4806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3908 4586 4945 5104 518 Size: 648 2742 678 359 159 2042 Fragments arranged by size: 2742 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2131 2557 2738 3055 3862 4044 4721 Size: 476 49 426 181 317 807 182 677 Cuts at: 4721 5869 6114 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 476 426 317 245 182 181 49 NaeI GCC'GGC Cuts at: 2060 2541 2600 2881 2060 Size: 481 59 281 5807 Fragments arranged by size: 5807 481 281 59 NarI GG'CG_CC Cuts at: 2056 2665 2056 Size: 609 6019 NcoI C'CATG_G Cuts at: 513 1065 2004 3786 513 Size: 552 939 1782 3355 Fragments arranged by size: 3355 1782 939 552 NdeI CA'TA_TG Cuts at: 387 3651 387 Size: 3264 3364 NgoMIV G'CCGG_C Cuts at: 2058 2539 2598 2879 2058 Size: 481 59 281 5807 Fragments arranged by size: 5807 481 281 59 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 3986 5531 1132 Size: 97 2757 1545 2229 Fragments arranged by size: 2757 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6628 PciI A'CATG_T Cuts at: 5527 5527 Size: 6628 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 1108 Size: 1680 4948 PfoI T'CCnGG_A Cuts at: 2111 2268 2111 Size: 157 6471 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 2791 4697 4729 6210 6242 2759 Size: 32 1906 32 1481 32 3145 Fragments arranged by size: 3145 1906 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3832 3832 Size: 6628 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PspOMI G'GGCC_C Cuts at: 2306 2490 3393 3621 2306 Size: 184 903 228 5313 Fragments arranged by size: 5313 903 228 184 PstI C_TGCA'G Cuts at: 830 2352 3054 3168 3980 830 Size: 1522 702 114 812 3478 Fragments arranged by size: 3478 1522 812 702 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 PvuII CAG'CTG Cuts at: 2131 3055 3862 2131 Size: 924 807 4897 Fragments arranged by size: 4897 924 807 RsrII CG'GwC_CG Cuts at: 2422 2422 Size: 6628 SalI G'TCGA_C Cuts at: 1936 2212 3970 1936 Size: 276 1758 4594 Fragments arranged by size: 4594 1758 276 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SfcI C'TryA_G Cuts at: 826 1045 2348 3050 3164 3976 5111 5792 5983 Size: 219 1303 702 114 812 1135 681 191 Cuts at: 5983 826 Size: 1471 Fragments arranged by size: 1471 1303 1135 812 702 681 219 191 114 SfoI GGC'GCC Cuts at: 2057 2666 2057 Size: 609 6019 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 2058 1700 Size: 358 6270 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2104 4732 5633 5895 820 Size: 197 926 161 2628 901 262 1553 Fragments arranged by size: 2628 1553 926 901 262 197 161 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 2327 3041 1517 Size: 810 714 5104 Fragments arranged by size: 5104 810 714 StyI C'CwwG_G Cuts at: 513 1065 2004 3786 3867 4067 513 Size: 552 939 1782 81 200 3074 Fragments arranged by size: 3074 1782 939 552 200 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2077 3671 4655 4672 4825 5010 5455 Size: 1255 497 1594 984 17 153 185 445 Cuts at: 5455 325 Size: 1498 Fragments arranged by size: 1594 1498 1255 984 497 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4874 6586 96 Size: 274 80 33 51 1443 2897 1712 138 Fragments arranged by size: 2897 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6588 6620 6588 Size: 32 6596 Tth111I GACn'n_nGTC Cuts at: 1316 2356 2503 1316 Size: 1040 147 5441 Fragments arranged by size: 5441 1040 147 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3313 3313 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 4757 4757 Size: 6628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BfrBI BplI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI