(Linear) (Six-base) MAPSORT of: KIEE3534.seq Check: 7386 from: 1 to: 1923 KIEE3534.orf With 182 enzymes: SgfI * July 3, 2012 18:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 501 1923 Size: 501 1422 AccI GT'mk_AC Cuts at: 0 208 1076 1923 Size: 208 868 847 Fragments arranged by size: 868 847 208 AclI AA'CG_TT Cuts at: 0 977 1923 Size: 977 946 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1113 1636 1923 Size: 1113 523 287 Fragments arranged by size: 1113 523 287 AfeI AGC'GCT Cuts at: 0 155 1923 Size: 155 1768 AhdI GACnn_n'nnGTC Cuts at: 0 1857 1923 Size: 1857 66 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1025 1793 1923 Size: 1025 768 130 Fragments arranged by size: 1025 768 130 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 754 786 1923 Size: 754 32 1137 Fragments arranged by size: 1137 754 32 AlwNI CAG_nnn'CTG Cuts at: 0 171 1923 Size: 171 1752 ApaI G_GGCC'C Cuts at: 0 305 489 1392 1620 1923 Size: 305 184 903 228 303 Fragments arranged by size: 903 305 303 228 184 ApaLI G'TGCA_C Cuts at: 0 447 1923 Size: 447 1476 AscI GG'CGCG_CC Cuts at: 0 358 1923 Size: 358 1565 AvaI C'yCGr_G Cuts at: 0 1085 1199 1364 1923 Size: 1085 114 165 559 Fragments arranged by size: 1085 559 165 114 BamHI G'GATC_C Cuts at: 0 1410 1923 Size: 1410 513 BanI G'GyrC_C Cuts at: 0 50 366 402 659 1923 Size: 50 316 36 257 1264 Fragments arranged by size: 1264 316 257 50 36 BanII G_rGCy'C Cuts at: 0 11 305 489 1092 1392 1620 1923 Size: 11 294 184 603 300 228 303 Fragments arranged by size: 603 303 300 294 228 184 11 BbeI G_GCGC'C Cuts at: 0 54 663 1923 Size: 54 609 1260 Fragments arranged by size: 1260 609 54 BbvCI CC'TCA_GC Cuts at: 0 157 1923 Size: 157 1766 BciVI GTATCCnnnnn_n' Cuts at: 0 150 1923 Size: 150 1773 BglI GCCn_nnn'nGGC Cuts at: 0 33 1923 Size: 33 1890 Bme1580I G_kGCm'C Cuts at: 0 305 371 407 451 489 1392 1560 1620 Size: 305 66 36 44 38 903 168 60 Cuts at: 1620 1923 Size: 303 Fragments arranged by size: 903 305 303 168 66 60 44 38 36 BmrI ACTGGGnnnn_n' Cuts at: 0 1614 1923 Size: 1614 309 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 1199 1616 1923 Size: 337 862 417 307 Fragments arranged by size: 862 417 337 307 Bpu10I CC'TnA_GC Cuts at: 0 157 1923 Size: 157 1766 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 1923 Size: 120 1803 BsaI GGTCTCn'nnnn_ Cuts at: 0 424 811 1923 Size: 424 387 1112 Fragments arranged by size: 1112 424 387 BsaAI yAC'GTr Cuts at: 0 1265 1923 Size: 1265 658 BsaHI Gr'CG_yC Cuts at: 0 51 499 660 788 1923 Size: 51 448 161 128 1135 Fragments arranged by size: 1135 448 161 128 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 754 784 1589 1619 1923 Size: 754 30 805 30 304 Fragments arranged by size: 805 754 304 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 775 883 1923 Size: 775 108 1040 Fragments arranged by size: 1040 775 108 BseYI C'CCAG_C Cuts at: 0 65 586 1391 1586 1766 1923 Size: 65 521 805 195 180 157 Fragments arranged by size: 805 521 195 180 157 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 787 1231 1339 1923 Size: 41 746 444 108 584 Fragments arranged by size: 746 584 444 108 41 BsiEI CG_ry'CG Cuts at: 0 46 215 260 470 837 1923 Size: 46 169 45 210 367 1086 Fragments arranged by size: 1086 367 210 169 46 45 BsiHKAI G_wGCw'C Cuts at: 0 451 1923 Size: 451 1472 BsmI GAATG_Cn' Cuts at: 0 1228 1395 1923 Size: 1228 167 528 Fragments arranged by size: 1228 528 167 BsmBI CGTCTCn'nnnn_ Cuts at: 0 895 1923 Size: 895 1028 Bsp1286I G_dGCh'C Cuts at: 0 11 305 371 407 451 489 1092 1392 Size: 11 294 66 36 44 38 603 300 Cuts at: 1392 1560 1620 1923 Size: 168 60 303 Fragments arranged by size: 603 303 300 294 168 66 60 44 38 36 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 501 1838 1923 Size: 501 1337 85 Fragments arranged by size: 1337 501 85 BsrBI CCG'CTC Cuts at: 0 604 793 1923 Size: 604 189 1130 Fragments arranged by size: 1130 604 189 BsrDI GCAATG_nn' Cuts at: 0 1334 1923 Size: 1334 589 BsrFI r'CCGG_y Cuts at: 0 53 414 534 593 656 874 1152 1923 Size: 53 361 120 59 63 218 278 771 Fragments arranged by size: 771 361 278 218 120 63 59 53 BssHII G'CGCG_C Cuts at: 0 334 358 388 839 1923 Size: 334 24 30 451 1084 Fragments arranged by size: 1084 451 334 30 24 BstAPI GCAn_nnn'nTGC Cuts at: 0 171 1167 1923 Size: 171 996 756 Fragments arranged by size: 996 756 171 BstXI CCAn_nnnn'nTGG Cuts at: 0 1586 1752 1923 Size: 1586 166 171 Fragments arranged by size: 1586 171 166 BstYI r'GATC_y Cuts at: 0 1410 1923 Size: 1410 513 BstZ17I GTA'TAC Cuts at: 0 1077 1923 Size: 1077 846 BtgI C'CryG_G Cuts at: 0 1781 1923 Size: 1781 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 612 1923 Size: 612 1311 BtsI GCAGTG_nn' Cuts at: 0 1322 1923 Size: 1322 601 DraIII CAC_nnn'GTG Cuts at: 0 1564 1923 Size: 1564 359 EaeI y'GGCC_r Cuts at: 0 43 212 257 291 467 566 774 834 Size: 43 169 45 34 176 99 208 60 Cuts at: 834 1490 1923 Size: 656 433 Fragments arranged by size: 656 433 208 176 169 99 60 45 43 34 EagI C'GGCC_G Cuts at: 0 43 212 257 467 834 1923 Size: 43 169 45 210 367 1089 Fragments arranged by size: 1089 367 210 169 45 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 1113 1199 1616 1636 1923 Size: 337 776 86 417 20 287 Fragments arranged by size: 776 417 337 287 86 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 397 868 1923 Size: 397 471 1055 Fragments arranged by size: 1055 471 397 EcoO109I rG'GnC_Cy Cuts at: 0 301 485 486 1388 1616 1827 1923 Size: 301 184 1 902 228 211 96 Fragments arranged by size: 902 301 228 211 184 96 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1442 1923 Size: 1442 481 FspI TGC'GCA Cuts at: 0 754 1923 Size: 754 1169 HaeII r_GCGC'y Cuts at: 0 54 157 663 1923 Size: 54 103 506 1260 Fragments arranged by size: 1260 506 103 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1843 1875 1923 Size: 1843 32 48 Fragments arranged by size: 1843 48 32 HincII GTy'rAC Cuts at: 0 209 1625 1923 Size: 209 1416 298 Fragments arranged by size: 1416 298 209 KasI G'GCGC_C Cuts at: 0 50 659 1923 Size: 50 609 1264 Fragments arranged by size: 1264 609 50 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 998 1923 Size: 998 925 MslI CAynn'nnrTG Cuts at: 0 1903 1923 Size: 1903 20 MspA1I CmG'CkG Cuts at: 0 77 126 552 733 1050 1857 1923 Size: 77 49 426 181 317 807 66 Fragments arranged by size: 807 426 317 181 77 66 49 NaeI GCC'GGC Cuts at: 0 55 536 595 876 1923 Size: 55 481 59 281 1047 Fragments arranged by size: 1047 481 281 59 55 NarI GG'CG_CC Cuts at: 0 51 660 1923 Size: 51 609 1263 Fragments arranged by size: 1263 609 51 NcoI C'CATG_G Cuts at: 0 1781 1923 Size: 1781 142 NdeI CA'TA_TG Cuts at: 0 1646 1923 Size: 1646 277 NgoMIV G'CCGG_C Cuts at: 0 53 534 593 874 1923 Size: 53 481 59 281 1049 Fragments arranged by size: 1049 481 281 59 53 PflMI CCAn_nnn'nTGG Cuts at: 0 783 1923 Size: 783 1140 PfoI T'CCnGG_A Cuts at: 0 106 263 1923 Size: 106 157 1660 Fragments arranged by size: 1660 157 106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 754 786 1923 Size: 754 32 1137 Fragments arranged by size: 1137 754 32 PpuMI rG'GwC_Cy Cuts at: 0 1827 1923 Size: 1827 96 PspOMI G'GGCC_C Cuts at: 0 301 485 1388 1616 1923 Size: 301 184 903 228 307 Fragments arranged by size: 903 307 301 228 184 PstI C_TGCA'G Cuts at: 0 347 1049 1163 1923 Size: 347 702 114 760 Fragments arranged by size: 760 702 347 114 PvuII CAG'CTG Cuts at: 0 126 1050 1857 1923 Size: 126 924 807 66 Fragments arranged by size: 924 807 126 66 RsrII CG'GwC_CG Cuts at: 0 417 1923 Size: 417 1506 SalI G'TCGA_C Cuts at: 0 207 1923 Size: 207 1716 SfcI C'TryA_G Cuts at: 0 343 1045 1159 1923 Size: 343 702 114 764 Fragments arranged by size: 764 702 343 114 SfoI GGC'GCC Cuts at: 0 52 661 1923 Size: 52 609 1262 Fragments arranged by size: 1262 609 52 SgrAI Cr'CCGG_yG Cuts at: 0 53 1923 Size: 53 1870 SmlI C'TyrA_G Cuts at: 0 99 1923 Size: 99 1824 StuI AGG'CCT Cuts at: 0 322 1036 1923 Size: 322 714 887 Fragments arranged by size: 887 714 322 StyI C'CwwG_G Cuts at: 0 1781 1862 1923 Size: 1781 81 61 Fragments arranged by size: 1781 81 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 72 1666 1923 Size: 72 1594 257 Fragments arranged by size: 1594 257 72 Tth111I GACn'n_nGTC Cuts at: 0 351 498 1923 Size: 351 147 1425 Fragments arranged by size: 1425 351 147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1308 1923 Size: 1308 615 Enzymes that do cut: AarI AccI AclI AcuI AfeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraIII EaeI EagI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV PflMI PfoI PpiI PpuMI PspOMI PstI PvuII RsrII SalI SfcI SfoI SgrAI SmlI StuI StyI TaqII Tth111I XcmI Enzymes that do not cut: AatII Acc65I AflII AflIII AgeI AleI ApoI AseI AsiSI AvrII BaeI BbsI BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI BsaWI BsiWI BspEI BspHI BsrGI BssSI BstBI BstEII Bsu36I ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI MscI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI