################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 522 GCGG CCGC(-3/-1) AcsI 516 AAATTT [GA]^AATT[CT] AflIII 950 ACATGT A^C[GA][CT]GT AlwNI 68 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 516 AAATTT [GA]^AATT[CT] AspI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 818 GGACC G^G[AT]CC BalI 779 TGGCCA TGG^CCA BbsI 910 GAAGAC GAAGAC(2/6) BclI 533 TGATCA T^GATCA BpuAI 910 GAAGAC GAAGAC(2/6) BsaAI 589 CACGTA [CT]AC^GT[GA] BsaI 481 GAGACC GGTCTC(1/5) BsmBI 746 GAGACG CGTCTC(1/5) BspLU11I 950 ACATGT A^CATGT BstBI 907 TTCGAA TT^CGAA DraII 1204 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 15 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1338 CCACGG C^C[GA][CT]GG EaeI 779 TGGCCA [CT]^GGCC[GA] EcoNI 729 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1204 AGGCCCC [GA]G^G[ACGT]CC[CT] FbaI 533 TGATCA T^GATCA HindIII 898 AAGCTT A^AGCTT MluNI 779 TGGCCA TGG^CCA MscI 779 TGGCCA TGG^CCA MslI 825 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspV 907 TTCGAA TT^CGAA PflFI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 577 GAAGAGC GCTCTTC(1/4) SfaNI 1304 GCATC GCATC(5/9) SfuI 907 TTCGAA TT^CGAA SmlI 634 CTCAAG C^T[CT][GA]AG SphI 360 GCATGC GCATG^C Tsp45I 76 GTCAC ^GT[GC]AC Tth111I 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 668 GTATAC GT^[AC][GT]AC 978 GTATAC AfaI 186 GTAC GT^AC 971 GTAC AspHI 34 GAGCTC G[AT]GC[AT]^C 995 GAGCTC BanII 34 GAGCTC G[GA]GC[CT]^C 995 GAGCTC BbvCI 787 CCTCAGC CCTCAGC(-5/-2) 1005 CCTCAGC BbvI 932 GCAGC GCAGC(8/12) 152 GCTGC BfaI 650 CTAG C^TAG 1219 CTAG BsiHKAI 34 GAGCTC G[AT]GC[AT]^C 995 GAGCTC BsmFI 107 GTCCC GGGAC(10/14) 241 GTCCC BspMI 40 ACCTGC ACCTGC(4/8) 728 ACCTGC Bst1107I 668 GTATAC GTA^TAC 978 GTATAC BstF5I 1035 GGATG GGATG(2/0) 1149 GGATG BstYI 1001 AGATCC [GA]^GATC[CT] 1342 GGATCT BstZ17I 668 GTATAC GTA^TAC 978 GTATAC Cfr13I 818 GGACC G^G[ACGT]CC 1205 GGCCC EarI 1165 CTCTTC CTCTTC(1/4) 577 GAAGAG Fnu4HI 152 GCTGC GC^[ACGT]GC 932 GCAGC FokI 1035 GGATG GGATG(9/13) 1149 GGATG HaeIII 780 GGCC GG^CC 1205 GGCC ItaI 152 GCTGC GC^[ACGT]GC 932 GCAGC Ksp632I 1165 CTCTTC CTCTTC(1/4) 577 GAAGAG MaeI 650 CTAG C^TAG 1219 CTAG MaeII 590 ACGT A^CGT 749 ACGT MaeIII 76 GTCAC ^GT[ACGT]AC 1185 GTTAC MflI 1001 AGATCC [GA]^GATC[CT] 1342 GGATCT MseI 845 TTAA T^TAA 1262 TTAA NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC 1205 GGCCCC PleI 1045 GACTC GAGTC(4/5) 1111 GACTC RsaI 186 GTAC GT^AC 971 GTAC SacI 34 GAGCTC GAGCT^C 995 GAGCTC Sau96I 818 GGACC G^G[ACGT]CC 1205 GGCCC SfcI 276 CTGTAG C^T[GA][CT]AG 405 CTATAG TaqI 46 TCGA T^CGA 908 TCGA TfiI 235 GAATC G^A[AT]TC 423 GATTC Tru9I 845 TTAA T^TAA 1262 TTAA TseI 152 GCTGC G^C[AT]GC 932 GCAGC TthHB8I 46 TCGA T^CGA 908 TCGA XhoII 1001 AGATCC [GA]^GATC[CT] 1342 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI AocI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Bsu36I Cac8I DdeI DpnII Eco81I EcoRII EcoT14I HinfI HphI MboI MboII MnlI MvaI MwoI NdeII NlaIII NspI Sau3AI ScrFI StyI Tsp509I TspRI