(Circular) (Six-base) MAPSORT of: pFN21AE3562.seq Check: 8696 from: 1 to: 6055 pFN21AE3562 6055 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 2054 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2674 2984 3398 1937 Size: 737 310 414 4594 Fragments arranged by size: 4594 737 414 310 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2723 2842 4118 5502 5770 1632 Size: 1091 119 1276 1384 268 1917 Fragments arranged by size: 1917 1384 1276 1091 268 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 2955 4954 5931 1203 Size: 1752 1999 977 1327 Fragments arranged by size: 1999 1752 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2834 1166 Size: 1668 4387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 2078 3798 5370 2078 Size: 1720 1572 2763 Fragments arranged by size: 2763 1720 1572 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2521 3364 3590 3742 5851 1493 Size: 1028 843 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 843 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3380 1870 Size: 73 1437 4545 Fragments arranged by size: 4545 1437 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3376 4831 618 Size: 325 217 627 1589 1455 1842 Fragments arranged by size: 1842 1589 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2043 3004 3374 1391 Size: 652 961 370 4072 Fragments arranged by size: 4072 961 652 370 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 928 Size: 971 1023 4061 Fragments arranged by size: 4061 1023 971 BbvCI CC'TCA_GC Cuts at: 2793 3011 2793 Size: 218 5837 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4246 4280 1903 Size: 34 2309 34 3678 Fragments arranged by size: 3678 2309 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 2538 1548 Size: 990 5065 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 2100 4116 5272 1790 Size: 310 2016 1156 2573 Fragments arranged by size: 2573 2016 1156 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2841 3040 3786 Size: 416 53 83 399 24 1424 199 746 Cuts at: 3786 4744 442 Size: 958 1753 Fragments arranged by size: 1753 1424 958 746 416 399 199 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4715 1239 Size: 3476 2579 Bpu10I CC'TnA_GC Cuts at: 2793 3011 2793 Size: 218 5837 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2624 4180 5045 5343 2624 Size: 1556 865 298 3336 Fragments arranged by size: 3336 1556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2480 4718 882 Size: 617 981 2238 2219 Fragments arranged by size: 2238 2219 981 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 3846 493 Size: 711 1392 1250 2702 Fragments arranged by size: 2702 1392 1250 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3862 4244 4879 275 Size: 53 83 186 936 2329 382 635 1451 Fragments arranged by size: 2329 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2131 2161 1178 Size: 30 165 30 728 30 5072 Fragments arranged by size: 5072 728 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6055 BseYI C'CCAG_C Cuts at: 5258 5754 5258 Size: 496 5559 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2041 2188 2956 1163 Size: 573 305 147 768 4262 Fragments arranged by size: 4262 768 573 305 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 3004 4116 4201 5272 2043 Size: 961 1112 85 1071 2826 Fragments arranged by size: 2826 1112 1071 961 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 BsmBI CGTCTCn'nnnn_ Cuts at: 2745 2745 Size: 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2100 3004 3374 4116 4201 5272 Size: 399 253 57 904 370 742 85 1071 Cuts at: 5272 1391 Size: 2174 Fragments arranged by size: 2174 1071 904 742 399 370 253 85 57 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2742 3410 844 Size: 1210 688 668 3489 Fragments arranged by size: 3489 1210 688 668 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4699 4886 1700 Size: 110 2889 187 2869 Fragments arranged by size: 2889 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 2913 4859 2913 Size: 1946 4109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3006 3347 3385 3752 4137 4154 5606 5692 Size: 1034 341 38 367 385 17 1452 86 Cuts at: 5692 5704 6050 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1034 385 367 346 341 86 38 17 12 BstZ17I GTA'TAC Cuts at: 2675 2985 2675 Size: 310 5745 Bsu36I CC'TnA_GG Cuts at: 2556 2739 2921 2556 Size: 183 182 5690 Fragments arranged by size: 5690 183 182 BtgI C'CryG_G Cuts at: 513 1065 2004 3343 513 Size: 552 939 1339 3225 Fragments arranged by size: 3225 1339 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2030 2939 3571 4365 4385 2030 Size: 909 632 794 20 3700 Fragments arranged by size: 3700 909 794 632 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DraIII CAC_nnn'GTG Cuts at: 2025 2025 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2784 3502 4391 Size: 54 1425 50 275 18 954 718 889 Cuts at: 4391 6020 8 Size: 1629 43 Fragments arranged by size: 1629 1425 954 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3176 3985 1353 Size: 1224 599 809 3423 Fragments arranged by size: 3423 1224 809 599 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 3002 2041 Size: 961 5094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2723 2842 4118 4715 5502 5770 1239 Size: 393 1091 119 1276 597 787 268 1524 Fragments arranged by size: 1524 1276 1091 787 597 393 268 119 EcoNI CCTnn'n_nnAGG Cuts at: 2738 2738 Size: 6055 EcoO109I rG'GnC_Cy Cuts at: 3210 3210 Size: 6055 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 5202 1678 Size: 3524 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2229 2261 2853 2885 2991 Size: 32 135 32 657 32 592 32 106 Cuts at: 2991 3023 4696 4728 4770 4802 1373 Size: 32 1673 32 42 32 2626 Fragments arranged by size: 2626 1673 657 592 135 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 2903 748 Size: 2155 3900 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2993 5169 5353 1305 Size: 1688 2176 184 2007 Fragments arranged by size: 2176 2007 1688 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 10 Size: 54 1425 343 954 3279 Fragments arranged by size: 3279 1425 954 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2834 4013 4372 4531 518 Size: 648 1668 1179 359 159 2042 Fragments arranged by size: 2042 1668 1179 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3471 4148 5296 5541 1606 Size: 1865 677 1148 245 2120 Fragments arranged by size: 2120 1865 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2369 2959 3109 3413 4958 1132 Size: 97 1140 590 150 304 1545 2229 Fragments arranged by size: 2229 1545 1140 590 304 150 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 2955 4954 2955 Size: 1999 4056 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PfoI T'CCnGG_A Cuts at: 3234 3234 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 2519 3625 2519 Size: 1106 4949 PstI C_TGCA'G Cuts at: 830 3407 830 Size: 2577 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 SacI G_AGCT'C Cuts at: 2043 3004 2043 Size: 961 5094 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SapI GCTCTTCn'nnn_ Cuts at: 2577 2577 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2281 2410 3403 4538 5219 5410 826 Size: 219 1236 129 993 1135 681 191 1471 Fragments arranged by size: 1471 1236 1135 993 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 4159 5060 5322 820 Size: 197 926 696 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 696 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 2369 3413 1132 Size: 1237 1044 3774 Fragments arranged by size: 3774 1237 1044 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2360 2874 3206 3214 3494 513 Size: 552 939 356 514 332 8 280 3074 Fragments arranged by size: 3074 939 552 514 356 332 280 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2975 4301 Size: 274 80 33 51 1443 213 785 1326 Cuts at: 4301 6013 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1326 785 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6015 6047 6015 Size: 32 6023 Tth111I GACn'n_nGTC Cuts at: 1316 2078 1316 Size: 762 5293 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI