################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AclI AgeI AhdI Aor51HI AscI AspEI AspI BbrPI BsiWI Bsp1407I BspDI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI HpaI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcyI 5732 GGCGCC G[GA]^CG[CT]C ApaI 5837 GGGCCC GGGCC^C AvaI 5129 CCCGAG C^[CT]CG[GA]G AviII 5678 TGCGCA TGC^GCA BamHI 510 GGATCC G^GATCC BbeI 5732 GGCGCC GGCGC^C BcgI 2384 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2543 TGATCA T^GATCA BlpI 2702 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2702 GCTCAGC GC^T[ACGT]AGC BsaBI 3622 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5732 GGCGCC G[GA]^CG[CT]C BsmBI 5228 GAGACG CGTCTC(1/5) BsoBI 5129 CCCGAG C^[CT]CG[GA]G Bst1107I 970 GTATAC GTA^TAC BstZ17I 970 GTATAC GTA^TAC CelII 2702 GCTCAGC GC^T[ACGT]AGC DraIII 5243 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 639 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4294 GAATTC G^AATTC FbaI 2543 TGATCA T^GATCA FspI 5678 TGCGCA TGC^GCA Hin1I 5732 GGCGCC G[GA]^CG[CT]C KasI 5732 GGCGCC G^GCGCC KspI 5860 CCGCGG CCGC^GG MamI 3622 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4415 CAATTG C^AATTG MunI 4415 CAATTG C^AATTG NarI 5732 GGCGCC GG^CGCC NdeI 65 CATATG CA^TATG SacII 5860 CCGCGG CCGC^GG SbfI 2409 CCTGCAGG CCTGCA^GG SexAI 479 ACCTGGT A^CC[AT]GGT SfoI 5732 GGCGCC GGC^GCC Sse8387I 2409 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 998 GGTACC G^GTACC 4867 GGTACC AccIII 513 TCCGGA T^CCGGA 4200 TCCGGA AflII 3987 CTTAAG C^TTAAG 4590 CTTAAG AflIII 974 ACATGT A^C[GA][CT]GT 5333 ACATGT AseI 3450 ATTAAT AT^TAAT 4492 ATTAAT AsnI 3450 ATTAAT AT^TAAT 4492 ATTAAT Asp700I 4059 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4290 GAATGAATTC Asp718I 998 GGTACC G^GTACC 4867 GGTACC AvrII 487 CCTAGG C^CTAGG 3162 CCTAGG BbvCI 2689 CCTCAGC CCTCAGC(-5/-2) 2658 GCTGAGG BfrI 3987 CTTAAG C^TTAAG 4590 CTTAAG BglI 1675 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5430 GCCTGCCGGGC BlnI 487 CCTAGG C^CTAGG 3162 CCTAGG BsaAI 2053 TACGTG [CT]AC^GT[GA] 2555 CACGTA BseAI 513 TCCGGA T^CCGGA 4200 TCCGGA BsiEI 408 CGACCG CG[GA][CT]^CG 843 CGGTCG BspEI 513 TCCGGA T^CCGGA 4200 TCCGGA BspLU11I 974 ACATGT A^CATGT 5333 ACATGT BssSI 19 CACGAG CACGAG(-5/-1) 4823 CTCGTG BstAPI 4338 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5202 GCAGAGGATGC DraI 1969 TTTAAA TTT^AAA 3307 TTTAAA DsaI 2444 CCGTGG C^C[GA][CT]GG 5860 CCGCGG HaeII 428 AGCGCC [GA]GCGC^[CT] 5732 GGCGCC HgaI 4886 GACGC GACGC(5/10) 5711 GCGTC HincII 4729 GTTGAC GT[CT]^[GA]AC 4912 GTCAAC HindII 4729 GTTGAC GT[CT]^[GA]AC 4912 GTCAAC KpnI 998 GGTACC GGTAC^C 4867 GGTACC MroI 513 TCCGGA T^CCGGA 4200 TCCGGA PpuMI 2537 AGGACCT [GA]G^G[AT]CC[CT] 4178 AGGTCCT PshBI 3450 ATTAAT AT^TAAT 4492 ATTAAT XmnI 4059 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4290 GAATGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII