(Circular) (Six-base) MAPSORT of: pFN21AE3553.seq Check: 3849 from: 1 to: 10603 pFN21AE3553 10603 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2377 5775 6859 2377 Size: 3398 1084 6121 Fragments arranged by size: 6121 3398 1084 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8413 278 Size: 53 83 186 936 6877 2468 Fragments arranged by size: 6877 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2976 3297 7719 7946 1937 Size: 1039 321 4422 227 4594 Fragments arranged by size: 4594 4422 1039 321 227 Acc65I G'GTAC_C Cuts at: 3003 6872 7924 3003 Size: 3869 1052 5682 Fragments arranged by size: 5682 3869 1052 AclI AA'CG_TT Cuts at: 1563 8730 9103 1563 Size: 7167 373 3063 Fragments arranged by size: 7167 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 3338 3596 4130 5938 6473 6627 6981 Size: 636 1070 258 534 1808 535 154 354 Cuts at: 6981 8666 10050 10318 1632 Size: 1685 1384 268 1917 Fragments arranged by size: 1917 1808 1685 1384 1070 636 535 534 354 268 258 154 AflII C'TTAA_G Cuts at: 820 1017 5992 6595 820 Size: 197 4975 603 4828 Fragments arranged by size: 4975 4828 603 197 AflIII A'CryG_T Cuts at: 1203 2979 7338 9502 10479 1203 Size: 1776 4359 2164 977 1327 Fragments arranged by size: 4359 2164 1776 1327 977 AgeI A'CCGG_T Cuts at: 9434 9434 Size: 10603 AhdI GACnn_n'nnGTC Cuts at: 9332 9332 Size: 10603 AleI CACnn'nnGTG Cuts at: 1166 2585 1166 Size: 1419 9184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6168 1184 Size: 4984 5619 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 10571 AlwNI CAG_nnn'CTG Cuts at: 2110 2392 5505 5660 6451 6664 6851 8346 9918 Size: 282 3113 155 791 213 187 1495 1572 Cuts at: 9918 2110 Size: 2795 Fragments arranged by size: 3113 2795 1572 1495 791 282 213 187 155 ApaI G_GGCC'C Cuts at: 7846 7846 Size: 10603 ApaLI G'TGCA_C Cuts at: 2831 3450 4557 8660 9816 2831 Size: 619 1107 4103 1156 3618 Fragments arranged by size: 4103 3618 1156 1107 619 ApoI r'AATT_y Cuts at: 1493 3239 3440 3879 3892 3943 3962 5145 5978 Size: 1746 201 439 13 51 19 1183 833 Cuts at: 5978 6248 6299 6668 7912 8138 8290 10399 1493 Size: 270 51 369 1244 226 152 2109 1697 Fragments arranged by size: 2109 1746 1697 1244 1183 833 439 369 270 226 201 152 51 51 19 13 AseI AT'TA_AT Cuts at: 160 1032 5456 6498 9157 160 Size: 872 4424 1042 2659 1606 Fragments arranged by size: 4424 2659 1606 1042 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10603 AvaI C'yCGr_G Cuts at: 1870 1943 7134 7928 1870 Size: 73 5191 794 4545 Fragments arranged by size: 5191 4545 794 73 AvrII C'CTAG_G Cuts at: 2492 5167 2492 Size: 2675 7928 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6761 6794 6864 6897 7407 7440 6761 Size: 33 70 33 510 33 9924 Fragments arranged by size: 9924 510 70 33 33 33 BamHI G'GATC_C Cuts at: 2515 7933 8300 2515 Size: 5418 367 4818 Fragments arranged by size: 5418 4818 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 3003 6872 7636 7648 Size: 325 217 627 701 515 3869 764 12 Cuts at: 7648 7737 7924 9379 618 Size: 89 187 1455 1842 Fragments arranged by size: 3869 1842 1455 764 701 627 515 325 217 187 89 12 BanII G_rGCy'C Cuts at: 1391 2544 3579 4301 5394 6866 7463 7846 7922 Size: 1153 1035 722 1093 1472 597 383 76 Cuts at: 7922 1391 Size: 4072 Fragments arranged by size: 4072 1472 1153 1093 1035 722 597 383 76 BbeI G_GCGC'C Cuts at: 7741 7741 Size: 10603 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2256 2529 6461 928 Size: 971 357 273 3932 5070 Fragments arranged by size: 5070 3932 971 357 273 BbvCI CC'TCA_GC Cuts at: 4664 4695 4664 Size: 31 10572 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 8794 8828 1903 Size: 34 2441 34 4382 34 3678 Fragments arranged by size: 4382 3678 2441 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2004 3163 3610 3850 3859 4574 6762 7435 8495 Size: 1159 447 240 9 715 2188 673 1060 Cuts at: 8495 9705 2004 Size: 1210 2902 Fragments arranged by size: 2902 2188 1210 1159 1060 715 673 447 240 9 BclI T'GATC_A Cuts at: 1548 4548 1548 Size: 3000 7603 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3686 7441 9214 Size: 107 122 71 631 605 2014 3755 1773 Cuts at: 9214 136 Size: 1525 Fragments arranged by size: 3755 2014 1773 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4033 5010 5242 10598 4033 Size: 977 232 5356 4038 Fragments arranged by size: 5356 4038 977 232 BlpI GC'TnA_GC Cuts at: 4708 8020 4708 Size: 3312 7291 Bme1580I G_kGCm'C Cuts at: 1790 2835 3454 4021 4561 7653 7846 8664 9820 Size: 1045 619 567 540 3092 193 818 1156 Cuts at: 9820 1790 Size: 2573 Fragments arranged by size: 3092 2573 1156 1045 818 619 567 540 193 BmgBI CAC'GTC Cuts at: 1318 2428 7581 7779 1318 Size: 1110 5153 198 4142 Fragments arranged by size: 5153 4142 1110 198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 2467 2483 Size: 416 53 83 399 24 882 168 16 Cuts at: 2483 4749 5296 8334 9292 442 Size: 2266 547 3038 958 1753 Fragments arranged by size: 3038 2266 1753 958 882 547 416 399 168 83 53 24 16 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10603 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3385 7065 7516 7716 3385 Size: 3680 451 200 6272 Fragments arranged by size: 6272 3680 451 200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2678 4284 4640 7327 7470 7853 9263 Size: 893 546 1606 356 2687 143 383 1410 Cuts at: 9263 1239 Size: 2579 Fragments arranged by size: 2687 2579 1606 1410 893 546 383 356 143 Bpu10I CC'TnA_GC Cuts at: 4568 4664 4695 5879 6763 4568 Size: 96 31 1184 884 8408 Fragments arranged by size: 8408 1184 884 96 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2740 6668 6687 7018 7060 8728 9593 9891 2740 Size: 3928 19 331 42 1668 865 298 3452 Fragments arranged by size: 3928 3452 1668 865 331 298 42 19 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6107 6706 7159 7897 9266 882 Size: 617 4608 599 453 738 1369 2219 Fragments arranged by size: 4608 2219 1369 738 617 599 453 BsaAI yAC'GTr Cuts at: 493 1204 4060 4562 8394 493 Size: 711 2856 502 3832 2702 Fragments arranged by size: 3832 2856 2702 711 502 BsaBI GATnn'nnATC Cuts at: 5631 10597 5631 Size: 4966 5637 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7738 8410 8792 9427 Size: 53 83 186 936 6205 672 382 635 Cuts at: 9427 275 Size: 1451 Fragments arranged by size: 6205 1451 936 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2415 2518 2761 4065 6205 9036 9434 9708 9855 Size: 103 243 1304 2140 2831 398 274 147 Cuts at: 9855 10266 2415 Size: 411 2752 Fragments arranged by size: 2831 2752 2140 1304 411 398 274 243 147 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 4500 4530 5474 Size: 30 165 30 682 30 2385 30 944 Cuts at: 5474 5504 5582 5612 5996 6026 6311 6341 7468 Size: 30 78 30 384 30 285 30 1127 Cuts at: 7468 7498 7748 7778 1178 Size: 30 250 30 4003 Fragments arranged by size: 4003 2385 1127 944 682 384 285 250 165 78 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 4205 5606 5663 6925 7688 1186 Size: 1480 1539 1401 57 1262 763 4101 Fragments arranged by size: 4101 1539 1480 1401 1262 763 57 BseYI C'CCAG_C Cuts at: 3169 4013 5235 6910 9806 10302 3169 Size: 844 1222 1675 2896 496 3470 Fragments arranged by size: 3470 2896 1675 1222 844 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 2934 5530 5753 6362 7254 7487 Size: 573 873 325 2596 223 609 892 233 Cuts at: 7487 7594 1163 Size: 107 4172 Fragments arranged by size: 4172 2596 892 873 609 573 325 233 223 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2416 2851 8053 8814 9842 1540 Size: 275 186 415 435 5202 761 1028 2301 Fragments arranged by size: 5202 2301 1028 761 435 415 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2544 2835 3454 3579 4050 4301 4561 5178 Size: 519 291 619 125 471 251 260 617 Cuts at: 5178 5394 5766 8664 8749 9820 2025 Size: 216 372 2898 85 1071 2808 Fragments arranged by size: 2898 2808 1071 619 617 519 471 372 291 260 251 216 125 85 BsmI GAATG_Cn' Cuts at: 2881 3043 4206 6063 6572 8114 8207 2881 Size: 162 1163 1857 509 1542 93 5277 Fragments arranged by size: 5277 1857 1542 1163 509 162 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7227 7227 Size: 10603 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2544 2835 3454 3579 4021 4050 Size: 399 235 519 291 619 125 442 29 Cuts at: 4050 4301 4561 5178 5394 5766 6866 7463 7653 Size: 251 260 617 216 372 1100 597 190 Cuts at: 7653 7846 7922 8664 8749 9820 1391 Size: 193 76 742 85 1071 2174 Fragments arranged by size: 2174 1100 1071 742 619 617 597 519 442 399 372 291 260 251 235 216 193 190 125 85 76 29 BspEI T'CCGG_A Cuts at: 2518 6205 10266 2518 Size: 3687 4061 2855 Fragments arranged by size: 4061 3687 2855 BspHI T'CATG_A Cuts at: 2358 3571 5256 5272 8492 10222 2358 Size: 1213 1685 16 3220 1730 2739 Fragments arranged by size: 3220 2739 1730 1685 1213 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2377 2943 3059 5775 6859 7958 844 Size: 1533 566 116 2716 1084 1099 3489 Fragments arranged by size: 3489 2716 1533 1099 1084 566 116 BsrBI CCG'CTC Cuts at: 1394 1823 4084 5425 7726 8490 1394 Size: 429 2261 1341 2301 764 3507 Fragments arranged by size: 3507 2301 2261 1341 764 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2470 2514 2770 4719 9098 9272 65 Size: 1183 1222 44 256 1949 4379 174 1396 Fragments arranged by size: 4379 1949 1396 1222 1183 256 174 44 BsrFI r'CCGG_y Cuts at: 1700 1810 2343 2694 7569 7633 7645 9247 9434 Size: 110 533 351 4875 64 12 1602 187 Cuts at: 9434 1700 Size: 2869 Fragments arranged by size: 4875 2869 1602 533 351 187 110 64 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10603 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10603 BssSI C'ACGA_G Cuts at: 2024 6828 8663 9675 2024 Size: 4804 1835 1012 2952 Fragments arranged by size: 4804 2952 1835 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6349 7213 794 Size: 5555 864 4184 Fragments arranged by size: 5555 4184 864 BstBI TT'CG_AA Cuts at: 9407 9407 Size: 10603 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10603 BstXI CCAn_nnnn'nTGG Cuts at: 3274 3433 3905 4651 4905 7810 7831 3274 Size: 159 472 746 254 2905 21 6046 Fragments arranged by size: 6046 2905 746 472 254 159 21 BstYI r'GATC_y Cuts at: 1972 2106 2515 4033 5010 5242 7933 8300 8685 Size: 134 409 1518 977 232 2691 367 385 Cuts at: 8685 8702 10154 10240 10252 10598 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2691 1977 1518 1452 977 409 385 367 346 232 134 86 17 12 BstZ17I GTA'TAC Cuts at: 2977 2977 Size: 10603 Bsu36I CC'TnA_GG Cuts at: 2378 5714 7591 2378 Size: 3336 1877 5390 Fragments arranged by size: 5390 3336 1877 BtgI C'CryG_G Cuts at: 513 1065 4449 7865 513 Size: 552 3384 3416 3251 Fragments arranged by size: 3416 3384 3251 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2911 6067 487 Size: 683 56 1685 3156 5023 Fragments arranged by size: 5023 3156 1685 683 56 BtsI GCAGTG_nn' Cuts at: 2806 4439 7180 7258 7555 8119 8913 8933 2806 Size: 1633 2741 78 297 564 794 20 4476 Fragments arranged by size: 4476 2741 1633 794 564 297 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10603 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7176 7211 529 Size: 35 6612 35 3921 Fragments arranged by size: 6612 3921 35 35 DraI TTT'AAA Cuts at: 3976 5314 7907 3976 Size: 1338 2593 6672 Fragments arranged by size: 6672 2593 1338 DraIII CAC_nnn'GTG Cuts at: 7253 7253 Size: 10603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9610 809 Size: 507 8294 1802 Fragments arranged by size: 8294 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2696 3264 3307 Size: 54 1425 50 275 18 866 568 43 Cuts at: 3307 4641 6678 8050 8939 10568 8 Size: 1334 2037 1372 889 1629 43 Fragments arranged by size: 2037 1629 1425 1372 1334 889 866 568 275 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8050 1537 Size: 275 6238 4090 Fragments arranged by size: 6238 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 2618 3251 3561 4182 5399 8533 1353 Size: 989 276 633 310 621 1217 3134 3423 Fragments arranged by size: 3423 3134 1217 989 633 621 310 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5314 7692 9190 9564 9710 387 Size: 1064 138 3725 2378 1498 374 146 1280 Fragments arranged by size: 3725 2378 1498 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2542 3577 4299 5392 2542 Size: 1035 722 1093 7753 Fragments arranged by size: 7753 1093 1035 722 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2268 2678 3338 3596 4130 4284 Size: 393 500 136 410 660 258 534 154 Cuts at: 4284 4640 5938 6473 6627 6981 7327 7470 7853 Size: 356 1298 535 154 354 346 143 383 Cuts at: 7853 8666 9263 10050 10318 1239 Size: 813 597 787 268 1524 Fragments arranged by size: 1524 1298 813 787 660 597 535 534 500 410 393 383 356 354 346 268 258 154 154 143 136 EcoNI CCTnn'n_nnAGG Cuts at: 2648 2648 Size: 10603 EcoO109I rG'GnC_Cy Cuts at: 4543 6184 7239 7842 7875 4543 Size: 1641 1055 603 33 7271 Fragments arranged by size: 7271 1641 1055 603 33 EcoRI G'AATT_C Cuts at: 6299 7912 8290 6299 Size: 1613 378 8612 Fragments arranged by size: 8612 1613 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3718 5002 6778 3718 Size: 1284 1776 7543 Fragments arranged by size: 7543 1776 1284 FspI TGC'GCA Cuts at: 7685 9109 7685 Size: 1424 9179 HaeII r_GCGC'y Cuts at: 1678 2437 7741 9750 1678 Size: 759 5304 2009 2531 Fragments arranged by size: 5304 2531 2009 759 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2545 2577 3314 3346 4023 Size: 32 135 32 973 32 737 32 677 Cuts at: 4023 4055 6311 6343 7546 7578 9244 9276 9318 Size: 32 2256 32 1203 32 1666 32 42 Cuts at: 9318 9350 1373 Size: 32 2626 Fragments arranged by size: 2626 2256 1666 1203 973 737 677 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6736 6919 7947 8193 669 Size: 988 281 4798 183 1028 246 3079 Fragments arranged by size: 4798 3079 1028 988 281 246 183 HindIII A'AGCT_T Cuts at: 748 5180 6220 6951 7036 748 Size: 4432 1040 731 85 4315 Fragments arranged by size: 4432 4315 1040 731 85 HpaI GTT'AAC Cuts at: 8193 8193 Size: 10603 KasI G'GCGC_C Cuts at: 7737 7737 Size: 10603 KpnI G_GTAC'C Cuts at: 3007 6876 7928 3007 Size: 3869 1052 5682 Fragments arranged by size: 5682 3869 1052 MfeI C'AATT_G Cuts at: 6420 8202 6420 Size: 1782 8821 MluI A'CGCG_T Cuts at: 10479 10479 Size: 10603 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 5049 5941 6073 7211 7323 7542 7563 Size: 1359 2385 892 132 1138 112 219 21 Cuts at: 7563 7761 9717 9901 1305 Size: 198 1956 184 2007 Fragments arranged by size: 2385 2007 1956 1359 1138 892 219 198 184 132 112 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 3266 3309 4643 10 Size: 54 1425 343 1434 43 1334 5970 Fragments arranged by size: 5970 1434 1425 1334 343 54 43 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2585 3431 3591 4043 4404 5513 Size: 648 1101 318 846 160 452 361 1109 Cuts at: 5513 6086 8561 8920 9079 518 Size: 573 2475 359 159 2042 Fragments arranged by size: 2475 2042 1109 1101 846 648 573 452 361 359 318 160 159 MspA1I CmG'CkG Cuts at: 1606 4200 6451 6914 7305 7867 8019 8696 9844 Size: 2594 2251 463 391 562 152 677 1148 Cuts at: 9844 10089 1606 Size: 245 2120 Fragments arranged by size: 2594 2251 2120 1148 677 562 463 391 245 152 NarI GG'CG_CC Cuts at: 7738 7738 Size: 10603 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 10051 NdeI CA'TA_TG Cuts at: 387 2071 387 Size: 1684 8919 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10603 NotI GC'GGCC_GC Cuts at: 8050 8050 Size: 10603 NspI r_CATG'y Cuts at: 1132 1229 2983 4403 5125 5158 7015 7342 7961 Size: 97 1754 1420 722 33 1857 327 619 Cuts at: 7961 9506 1132 Size: 1545 2229 Fragments arranged by size: 2229 1857 1754 1545 1420 722 619 327 97 33 PasI CC'CwG_GG Cuts at: 1348 4375 1348 Size: 3027 7576 PciI A'CATG_T Cuts at: 2979 7338 9502 2979 Size: 4359 2164 4080 Fragments arranged by size: 4359 4080 2164 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10603 PfoI T'CCnGG_A Cuts at: 2109 2370 4098 4344 4365 4728 2109 Size: 261 1728 246 21 363 7984 Fragments arranged by size: 7984 1728 363 261 246 21 PmeI GTTT'AAAC Cuts at: 7907 7907 Size: 10603 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2049 2081 2085 2117 6186 6218 8672 8704 10185 Size: 32 4 32 4069 32 2454 32 1481 Cuts at: 10185 10217 2049 Size: 32 2435 Fragments arranged by size: 4069 2454 2435 1481 32 32 32 32 32 4 PpuMI rG'GwC_Cy Cuts at: 4543 6184 4543 Size: 1641 8962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10603 PsiI TTA'TAA Cuts at: 3048 5007 5048 8173 3048 Size: 1959 41 3125 5478 Fragments arranged by size: 5478 3125 1959 41 PspOMI G'GGCC_C Cuts at: 7842 7842 Size: 10603 PstI C_TGCA'G Cuts at: 830 3855 4419 4434 4854 5067 6192 6541 7955 Size: 3025 564 15 420 213 1125 349 1414 Cuts at: 7955 830 Size: 3478 Fragments arranged by size: 3478 3025 1414 1125 564 420 349 213 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10603 PvuII CAG'CTG Cuts at: 6451 6914 7305 6451 Size: 463 391 9749 Fragments arranged by size: 9749 463 391 SacI G_AGCT'C Cuts at: 2544 3579 4301 5394 2544 Size: 1035 722 1093 7753 Fragments arranged by size: 7753 1093 1035 722 SacII CC_GC'GG Cuts at: 7868 7868 Size: 10603 SalI G'TCGA_C Cuts at: 1936 7945 1936 Size: 6009 4594 SapI GCTCTTCn'nnn_ Cuts at: 2618 3251 5399 2618 Size: 633 2148 7822 Fragments arranged by size: 7822 2148 633 SbfI CC_TGCA'GG Cuts at: 4419 7955 4419 Size: 3536 7067 ScaI AGT'ACT Cuts at: 8851 10563 8851 Size: 1712 8891 SexAI A'CCwGG_T Cuts at: 2484 2484 Size: 10603 SfcI C'TryA_G Cuts at: 826 1045 2881 3851 4415 4430 4850 5063 5869 Size: 219 1836 970 564 15 420 213 806 Cuts at: 5869 6188 6537 7543 7951 9086 9767 9958 826 Size: 319 349 1006 408 1135 681 191 1471 Fragments arranged by size: 1836 1471 1135 1006 970 806 681 564 420 408 349 319 219 213 191 15 SfoI GGC'GCC Cuts at: 7739 7739 Size: 10603 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10603 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10603 SmaI CCC'GGG Cuts at: 1872 7930 1872 Size: 6058 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2719 5992 6595 6647 6702 7033 Size: 197 926 776 3273 603 52 55 331 Cuts at: 7033 7039 8707 9608 9870 820 Size: 6 1668 901 262 1553 Fragments arranged by size: 3273 1668 1553 926 901 776 603 331 262 197 55 52 6 SnaBI TAC'GTA Cuts at: 493 8394 493 Size: 7901 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10603 SphI G_CATG'C Cuts at: 1132 7961 1132 Size: 6829 3774 SspI AAT'ATT Cuts at: 5 52 3128 3184 4875 6496 6973 8527 5 Size: 47 3076 56 1691 1621 477 1554 2081 Fragments arranged by size: 3076 2081 1691 1621 1554 477 56 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10603 StyI C'CwwG_G Cuts at: 513 1065 2492 3648 3661 5167 8042 513 Size: 552 1427 1156 13 1506 2875 3074 Fragments arranged by size: 3074 2875 1506 1427 1156 552 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 2837 3798 6276 6304 8630 8647 Size: 1255 843 414 961 2478 28 2326 17 Cuts at: 8647 8800 8985 9430 325 Size: 153 185 445 1498 Fragments arranged by size: 2478 2326 1498 1255 961 843 445 414 185 153 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4314 8849 10561 Size: 274 80 33 51 1443 2337 4535 1712 Cuts at: 10561 96 Size: 138 Fragments arranged by size: 4535 2337 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5996 6028 7115 7147 7468 7500 7748 7780 10563 Size: 32 1087 32 321 32 248 32 2783 Cuts at: 10563 10595 5996 Size: 32 6004 Fragments arranged by size: 6004 2783 1087 321 248 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10603 XbaI T'CTAG_A Cuts at: 7939 7939 Size: 10603 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4399 4736 6815 7835 4399 Size: 337 2079 1020 7167 Fragments arranged by size: 7167 2079 1020 337 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10603 XmaI C'CCGG_G Cuts at: 1870 7928 1870 Size: 6058 4545 XmnI GAAnn'nnTTC Cuts at: 6068 6299 8732 6068 Size: 231 2433 7939 Fragments arranged by size: 7939 2433 231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8411 276 Size: 53 83 186 936 6877 2468 Fragments arranged by size: 6877 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BsiWI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI