(Linear) (Six-base) MAPSORT of: KIEE3553.seq Check: 1815 from: 1 to: 5898 KIEE3553.orf With 182 enzymes: SgfI * July 3, 2012 18:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 372 3770 4854 5898 Size: 372 3398 1084 1044 Fragments arranged by size: 3398 1084 1044 372 AccI GT'mk_AC Cuts at: 0 971 1292 5714 5898 Size: 971 321 4422 184 Fragments arranged by size: 4422 971 321 184 Acc65I G'GTAC_C Cuts at: 0 998 4867 5898 Size: 998 3869 1031 Fragments arranged by size: 3869 1031 998 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 1333 1591 2125 3933 4468 4622 4976 Size: 263 1070 258 534 1808 535 154 354 Cuts at: 4976 5898 Size: 922 Fragments arranged by size: 1808 1070 922 535 534 354 263 258 154 AflII C'TTAA_G Cuts at: 0 3987 4590 5898 Size: 3987 603 1308 Fragments arranged by size: 3987 1308 603 AflIII A'CryG_T Cuts at: 0 974 5333 5898 Size: 974 4359 565 Fragments arranged by size: 4359 974 565 AleI CACnn'nnGTG Cuts at: 0 580 5898 Size: 580 5318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4163 5898 Size: 4163 1735 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 80 112 5898 Size: 80 32 5786 Fragments arranged by size: 5786 80 32 AlwNI CAG_nnn'CTG Cuts at: 0 105 387 3500 3655 4446 4659 4846 5898 Size: 105 282 3113 155 791 213 187 1052 Fragments arranged by size: 3113 1052 791 282 213 187 155 105 ApaI G_GGCC'C Cuts at: 0 5841 5898 Size: 5841 57 ApaLI G'TGCA_C Cuts at: 0 826 1445 2552 5898 Size: 826 619 1107 3346 Fragments arranged by size: 3346 1107 826 619 ApoI r'AATT_y Cuts at: 0 1234 1435 1874 1887 1938 1957 3140 3973 Size: 1234 201 439 13 51 19 1183 833 Cuts at: 3973 4243 4294 4663 5898 Size: 270 51 369 1235 Fragments arranged by size: 1235 1234 1183 833 439 369 270 201 51 51 19 13 AseI AT'TA_AT Cuts at: 0 3451 4493 5898 Size: 3451 1042 1405 Fragments arranged by size: 3451 1405 1042 AvaI C'yCGr_G Cuts at: 0 5129 5898 Size: 5129 769 AvrII C'CTAG_G Cuts at: 0 487 3162 5898 Size: 487 2675 2736 Fragments arranged by size: 2736 2675 487 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 4756 4789 4859 4892 5402 5435 5898 Size: 4756 33 70 33 510 33 463 Fragments arranged by size: 4756 510 463 70 33 33 33 BamHI G'GATC_C Cuts at: 0 510 5898 Size: 510 5388 BanI G'GyrC_C Cuts at: 0 483 998 4867 5631 5643 5732 5898 Size: 483 515 3869 764 12 89 166 Fragments arranged by size: 3869 764 515 483 166 89 12 BanII G_rGCy'C Cuts at: 0 539 1574 2296 3389 4861 5458 5841 5898 Size: 539 1035 722 1093 1472 597 383 57 Fragments arranged by size: 1472 1093 1035 722 597 539 383 57 BbeI G_GCGC'C Cuts at: 0 5736 5898 Size: 5736 162 BbsI GAAGACnn'nnnn_ Cuts at: 0 251 524 4456 5898 Size: 251 273 3932 1442 Fragments arranged by size: 3932 1442 273 251 BbvCI CC'TCA_GC Cuts at: 0 2659 2690 5898 Size: 2659 31 3208 Fragments arranged by size: 3208 2659 31 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2373 2407 5898 Size: 2373 34 3491 Fragments arranged by size: 3491 2373 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1158 1605 1845 1854 2569 4757 5430 5898 Size: 1158 447 240 9 715 2188 673 468 Fragments arranged by size: 2188 1158 715 673 468 447 240 9 BclI T'GATC_A Cuts at: 0 2543 5898 Size: 2543 3355 BglI GCCn_nnn'nGGC Cuts at: 0 1681 5436 5898 Size: 1681 3755 462 Fragments arranged by size: 3755 1681 462 BglII A'GATC_T Cuts at: 0 2028 3005 3237 5898 Size: 2028 977 232 2661 Fragments arranged by size: 2661 2028 977 232 BlpI GC'TnA_GC Cuts at: 0 2703 5898 Size: 2703 3195 Bme1580I G_kGCm'C Cuts at: 0 830 1449 2016 2556 5648 5841 5898 Size: 830 619 567 540 3092 193 57 Fragments arranged by size: 3092 830 619 567 540 193 57 BmgBI CAC'GTC Cuts at: 0 423 5576 5774 5898 Size: 423 5153 198 124 Fragments arranged by size: 5153 423 198 124 BmrI ACTGGGnnnn_n' Cuts at: 0 294 462 478 2744 3291 5898 Size: 294 168 16 2266 547 2607 Fragments arranged by size: 2607 2266 547 294 168 16 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1380 5060 5511 5711 5898 Size: 1380 3680 451 200 187 Fragments arranged by size: 3680 1380 451 200 187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 673 2279 2635 5322 5465 5848 5898 Size: 127 546 1606 356 2687 143 383 50 Fragments arranged by size: 2687 1606 546 383 356 143 127 50 Bpu10I CC'TnA_GC Cuts at: 0 2563 2659 2690 3874 4758 5898 Size: 2563 96 31 1184 884 1140 Fragments arranged by size: 2563 1184 1140 884 96 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 735 4663 4682 5013 5055 5898 Size: 735 3928 19 331 42 843 Fragments arranged by size: 3928 843 735 331 42 19 BsaI GGTCTCn'nnnn_ Cuts at: 0 4102 4701 5154 5892 5898 Size: 4102 599 453 738 6 Fragments arranged by size: 4102 738 599 453 6 BsaAI yAC'GTr Cuts at: 0 2055 2557 5898 Size: 2055 502 3341 Fragments arranged by size: 3341 2055 502 BsaBI GATnn'nnATC Cuts at: 0 3626 5898 Size: 3626 2272 BsaHI Gr'CG_yC Cuts at: 0 5733 5898 Size: 5733 165 BsaWI w'CCGG_w Cuts at: 0 410 513 756 2060 4200 5898 Size: 410 103 243 1304 2140 1698 Fragments arranged by size: 2140 1698 1304 410 243 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 80 110 2495 2525 3469 3499 3577 3607 Size: 80 30 2385 30 944 30 78 30 Cuts at: 3607 3991 4021 4306 4336 5463 5493 5743 5773 Size: 384 30 285 30 1127 30 250 30 Cuts at: 5773 5898 Size: 125 Fragments arranged by size: 2385 1127 944 384 285 250 125 80 78 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 661 2200 3601 3658 4920 5683 5898 Size: 661 1539 1401 57 1262 763 215 Fragments arranged by size: 1539 1401 1262 763 661 215 57 BseYI C'CCAG_C Cuts at: 0 1164 2008 3230 4905 5898 Size: 1164 844 1222 1675 993 Fragments arranged by size: 1675 1222 1164 993 844 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 929 3525 3748 4357 5249 5482 5589 Size: 604 325 2596 223 609 892 233 107 Cuts at: 5589 5898 Size: 309 Fragments arranged by size: 2596 892 609 604 325 309 233 223 107 BsiEI CG_ry'CG Cuts at: 0 411 846 5898 Size: 411 435 5052 Fragments arranged by size: 5052 435 411 BsiHKAI G_wGCw'C Cuts at: 0 20 539 830 1449 1574 2045 2296 2556 Size: 20 519 291 619 125 471 251 260 Cuts at: 2556 3173 3389 3761 5898 Size: 617 216 372 2137 Fragments arranged by size: 2137 619 617 519 471 372 291 260 251 216 125 20 BsmI GAATG_Cn' Cuts at: 0 876 1038 2201 4058 4567 5898 Size: 876 162 1163 1857 509 1331 Fragments arranged by size: 1857 1331 1163 876 509 162 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5222 5898 Size: 5222 676 Bsp1286I G_dGCh'C Cuts at: 0 20 539 830 1449 1574 2016 2045 2296 Size: 20 519 291 619 125 442 29 251 Cuts at: 2296 2556 3173 3389 3761 4861 5458 5648 5841 Size: 260 617 216 372 1100 597 190 193 Cuts at: 5841 5898 Size: 57 Fragments arranged by size: 1100 619 617 597 519 442 372 291 260 251 216 193 190 125 57 29 20 BspEI T'CCGG_A Cuts at: 0 513 4200 5898 Size: 513 3687 1698 Fragments arranged by size: 3687 1698 513 BspHI T'CATG_A Cuts at: 0 353 1566 3251 3267 5898 Size: 353 1213 1685 16 2631 Fragments arranged by size: 2631 1685 1213 353 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 372 938 1054 3770 4854 5898 Size: 372 566 116 2716 1084 1044 Fragments arranged by size: 2716 1084 1044 566 372 116 BsrBI CCG'CTC Cuts at: 0 2079 3420 5721 5898 Size: 2079 1341 2301 177 Fragments arranged by size: 2301 2079 1341 177 BsrDI GCAATG_nn' Cuts at: 0 465 509 765 2714 5898 Size: 465 44 256 1949 3184 Fragments arranged by size: 3184 1949 465 256 44 BsrFI r'CCGG_y Cuts at: 0 338 689 5564 5628 5640 5898 Size: 338 351 4875 64 12 258 Fragments arranged by size: 4875 351 338 258 64 12 BssSI C'ACGA_G Cuts at: 0 19 4823 5898 Size: 19 4804 1075 Fragments arranged by size: 4804 1075 19 BstAPI GCAn_nnn'nTGC Cuts at: 0 4344 5208 5898 Size: 4344 864 690 Fragments arranged by size: 4344 864 690 BstXI CCAn_nnnn'nTGG Cuts at: 0 1269 1428 1900 2646 2900 5805 5826 5898 Size: 1269 159 472 746 254 2905 21 72 Fragments arranged by size: 2905 1269 746 472 254 159 72 21 BstYI r'GATC_y Cuts at: 0 101 510 2028 3005 3237 5898 Size: 101 409 1518 977 232 2661 Fragments arranged by size: 2661 1518 977 409 232 101 BstZ17I GTA'TAC Cuts at: 0 972 5898 Size: 972 4926 Bsu36I CC'TnA_GG Cuts at: 0 373 3709 5586 5898 Size: 373 3336 1877 312 Fragments arranged by size: 3336 1877 373 312 BtgI C'CryG_G Cuts at: 0 2444 5860 5898 Size: 2444 3416 38 Fragments arranged by size: 3416 2444 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 906 4062 5898 Size: 906 3156 1836 Fragments arranged by size: 3156 1836 906 BtsI GCAGTG_nn' Cuts at: 0 801 2434 5175 5253 5550 5898 Size: 801 1633 2741 78 297 348 Fragments arranged by size: 2741 1633 801 348 297 78 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 5171 5206 5898 Size: 5171 35 692 Fragments arranged by size: 5171 692 35 DraI TTT'AAA Cuts at: 0 1971 3309 5898 Size: 1971 1338 2589 Fragments arranged by size: 2589 1971 1338 DraIII CAC_nnn'GTG Cuts at: 0 5248 5898 Size: 5248 650 EaeI y'GGCC_r Cuts at: 0 691 1259 1302 2636 4673 5898 Size: 691 568 43 1334 2037 1225 Fragments arranged by size: 2037 1334 1225 691 568 43 EarI CTCTTCn'nnn_ Cuts at: 0 337 613 1246 1556 2177 3394 5898 Size: 337 276 633 310 621 1217 2504 Fragments arranged by size: 2504 1217 633 621 337 310 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3309 5687 5898 Size: 3309 2378 211 Fragments arranged by size: 3309 2378 211 EcoICRI GAG'CTC Cuts at: 0 537 1572 2294 3387 5898 Size: 537 1035 722 1093 2511 Fragments arranged by size: 2511 1093 1035 722 537 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 263 673 1333 1591 2125 2279 2635 Size: 127 136 410 660 258 534 154 356 Cuts at: 2635 3933 4468 4622 4976 5322 5465 5848 5898 Size: 1298 535 154 354 346 143 383 50 Fragments arranged by size: 1298 660 535 534 410 383 356 354 346 258 154 154 143 136 127 50 EcoNI CCTnn'n_nnAGG Cuts at: 0 643 5898 Size: 643 5255 EcoO109I rG'GnC_Cy Cuts at: 0 2538 4179 5234 5837 5870 5898 Size: 2538 1641 1055 603 33 28 Fragments arranged by size: 2538 1641 1055 603 33 28 EcoRI G'AATT_C Cuts at: 0 4294 5898 Size: 4294 1604 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1713 2997 4773 5898 Size: 1713 1284 1776 1125 Fragments arranged by size: 1776 1713 1284 1125 FspI TGC'GCA Cuts at: 0 5680 5898 Size: 5680 218 HaeII r_GCGC'y Cuts at: 0 432 5736 5898 Size: 432 5304 162 Fragments arranged by size: 5304 432 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 540 572 1309 1341 2018 2050 4306 4338 Size: 540 32 737 32 677 32 2256 32 Cuts at: 4338 5541 5573 5898 Size: 1203 32 325 Fragments arranged by size: 2256 1203 737 677 540 325 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 4731 4914 5898 Size: 4731 183 984 Fragments arranged by size: 4731 984 183 HindIII A'AGCT_T Cuts at: 0 3175 4215 4946 5031 5898 Size: 3175 1040 731 85 867 Fragments arranged by size: 3175 1040 867 731 85 KasI G'GCGC_C Cuts at: 0 5732 5898 Size: 5732 166 KpnI G_GTAC'C Cuts at: 0 1002 4871 5898 Size: 1002 3869 1027 Fragments arranged by size: 3869 1027 1002 MfeI C'AATT_G Cuts at: 0 4415 5898 Size: 4415 1483 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 659 3044 3936 4068 5206 5318 5537 5558 Size: 659 2385 892 132 1138 112 219 21 Cuts at: 5558 5756 5898 Size: 198 142 Fragments arranged by size: 2385 1138 892 659 219 198 142 132 112 21 MscI TGG'CCA Cuts at: 0 1261 1304 2638 5898 Size: 1261 43 1334 3260 Fragments arranged by size: 3260 1334 1261 43 MslI CAynn'nnrTG Cuts at: 0 262 580 1426 1586 2038 2399 3508 4081 Size: 262 318 846 160 452 361 1109 573 Cuts at: 4081 5898 Size: 1817 Fragments arranged by size: 1817 1109 846 573 452 361 318 262 160 MspA1I CmG'CkG Cuts at: 0 2195 4446 4909 5300 5862 5898 Size: 2195 2251 463 391 562 36 Fragments arranged by size: 2251 2195 562 463 391 36 NarI GG'CG_CC Cuts at: 0 5733 5898 Size: 5733 165 NdeI CA'TA_TG Cuts at: 0 66 5898 Size: 66 5832 NspI r_CATG'y Cuts at: 0 978 2398 3120 3153 5010 5337 5898 Size: 978 1420 722 33 1857 327 561 Fragments arranged by size: 1857 1420 978 722 561 327 33 PasI CC'CwG_GG Cuts at: 0 2370 5898 Size: 2370 3528 PciI A'CATG_T Cuts at: 0 974 5333 5898 Size: 974 4359 565 Fragments arranged by size: 4359 974 565 PfoI T'CCnGG_A Cuts at: 0 104 365 2093 2339 2360 2723 5898 Size: 104 261 1728 246 21 363 3175 Fragments arranged by size: 3175 1728 363 261 246 104 21 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 44 76 80 112 4181 4213 5898 Size: 44 32 4 32 4069 32 1685 Fragments arranged by size: 4069 1685 44 32 32 32 4 PpuMI rG'GwC_Cy Cuts at: 0 2538 4179 5898 Size: 2538 1641 1719 Fragments arranged by size: 2538 1719 1641 PsiI TTA'TAA Cuts at: 0 1043 3002 3043 5898 Size: 1043 1959 41 2855 Fragments arranged by size: 2855 1959 1043 41 PspOMI G'GGCC_C Cuts at: 0 5837 5898 Size: 5837 61 PstI C_TGCA'G Cuts at: 0 1850 2414 2429 2849 3062 4187 4536 5898 Size: 1850 564 15 420 213 1125 349 1362 Fragments arranged by size: 1850 1362 1125 564 420 349 213 15 PvuII CAG'CTG Cuts at: 0 4446 4909 5300 5898 Size: 4446 463 391 598 Fragments arranged by size: 4446 598 463 391 SacI G_AGCT'C Cuts at: 0 539 1574 2296 3389 5898 Size: 539 1035 722 1093 2509 Fragments arranged by size: 2509 1093 1035 722 539 SacII CC_GC'GG Cuts at: 0 5863 5898 Size: 5863 35 SapI GCTCTTCn'nnn_ Cuts at: 0 613 1246 3394 5898 Size: 613 633 2148 2504 Fragments arranged by size: 2504 2148 633 613 SbfI CC_TGCA'GG Cuts at: 0 2414 5898 Size: 2414 3484 SexAI A'CCwGG_T Cuts at: 0 479 5898 Size: 479 5419 SfcI C'TryA_G Cuts at: 0 876 1846 2410 2425 2845 3058 3864 4183 Size: 876 970 564 15 420 213 806 319 Cuts at: 4183 4532 5538 5898 Size: 349 1006 360 Fragments arranged by size: 1006 970 876 806 564 420 360 349 319 213 15 SfoI GGC'GCC Cuts at: 0 5734 5898 Size: 5734 164 SmlI C'TyrA_G Cuts at: 0 714 3987 4590 4642 4697 5028 5034 5898 Size: 714 3273 603 52 55 331 6 864 Fragments arranged by size: 3273 864 714 603 331 55 52 6 SspI AAT'ATT Cuts at: 0 1123 1179 2870 4491 4968 5898 Size: 1123 56 1691 1621 477 930 Fragments arranged by size: 1691 1621 1123 930 477 56 StyI C'CwwG_G Cuts at: 0 487 1643 1656 3162 5898 Size: 487 1156 13 1506 2736 Fragments arranged by size: 2736 1506 1156 487 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 418 832 1793 4271 4299 5898 Size: 418 414 961 2478 28 1599 Fragments arranged by size: 2478 1599 961 418 414 28 TatI w'GTAC_w Cuts at: 0 2309 5898 Size: 2309 3589 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3991 4023 5110 5142 5463 5495 5743 5775 Size: 3991 32 1087 32 321 32 248 32 Cuts at: 5775 5898 Size: 123 Fragments arranged by size: 3991 1087 321 248 123 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2394 2731 4810 5830 5898 Size: 2394 337 2079 1020 68 Fragments arranged by size: 2394 2079 1020 337 68 XmnI GAAnn'nnTTC Cuts at: 0 4063 4294 5898 Size: 4063 231 1604 Fragments arranged by size: 4063 1604 231 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I NarI NdeI NspI PasI PciI PfoI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfoI SmlI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AgeI AhdI AscI AsiSI BfrBI BmtI BsiWI BsrGI BssHII BstBI BstEII ClaI DrdI EagI EcoRV FseI FspAI HpaI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PshAI PspXI PsrI PvuI RsrII SalI SanDI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI