################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AlwNI ApaI AscI AseI AsnI Asp718I AspI AviII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflII 841 CTTAAG C^TTAAG AflIII 815 ACATGT A^C[GA][CT]GT AhdI 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1609 GTGCAC G^TGCAC AocI 1306 CCTGAGG CC^T[ACGT]AGG Aor51HI 7 AGCGCT AGC^GCT ApaLI 1609 GTGCAC G^TGCAC AspEI 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 705 CCTAGG C^CTAGG BamHI 736 GGATCC G^GATCC BfrI 841 CTTAAG C^TTAAG BglI 1534 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 705 CCTAGG C^CTAGG BsmBI 1966 GAGACG CGTCTC(1/5) BspDI 902 ATCGAT AT^CGAT BspHI 893 TCATGA T^CATGA BspLU11I 815 ACATGT A^CATGT BstAPI 1821 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG ClaI 902 ATCGAT AT^CGAT DraIII 1981 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 7 AGCGCT AGC^GCT Eco81I 1306 CCTGAGG CC^T[ACGT]AGG EcoNI 1476 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2271 GATATC GAT^ATC HindIII 2329 AAGCTT A^AGCTT NaeI 1874 GCCGGC GCC^GGC NgoMIV 1874 GCCGGC G^CCGGC PaeR7I 455 CTCGAG C^TCGAG RcaI 893 TCATGA T^CATGA ScaI 491 AGTACT AGT^ACT SphI 2174 GCATGC GCATG^C XhoI 455 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Asp700I 285 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2017 GAAGAATTTC BalI 1064 TGGCCA TGG^CCA 1860 TGGCCA BbrPI 1981 CACGTG CAC^GTG 2055 CACGTG BsgI 487 GTGCAG GTGCAG(16/14) 2206 GTGCAG BspMI 539 ACCTGC ACCTGC(4/8) 836 ACCTGC BsrBI 2040 CCGCTC CCGCTC(-3/-3) 1465 GAGCGG BsrDI 2301 CATTGC GCAATG(2/0) 590 GCAATG BsrFI 158 ACCGGC [GA]^CCGG[CT] 1874 GCCGGC BsrI 223 ACTGG ACTGG(1/-1) 726 ACTGG BssSI 2073 CACGAG CACGAG(-5/-1) 2194 CACGAG Cfr10I 158 ACCGGC [GA]^CCGG[CT] 1874 GCCGGC EarI 658 CTCTTC CTCTTC(1/4) 1018 GAAGAG EcoRI 595 GAATTC G^AATTC 1449 GAATTC Ksp632I 658 CTCTTC CTCTTC(1/4) 1018 GAAGAG MluNI 1064 TGGCCA TGG^CCA 1860 TGGCCA MscI 1064 TGGCCA TGG^CCA 1860 TGGCCA MslI 922 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2280 CACCTCCATG PmaCI 1981 CACGTG CAC^GTG 2055 CACGTG PmlI 1981 CACGTG CAC^GTG 2055 CACGTG PpuMI 971 AGGACCC [GA]G^G[AT]CC[CT] 1525 GGGTCCC PvuII 1139 CAGCTG CAG^CTG 2133 CAGCTG SacI 1092 GAGCTC GAGCT^C 2076 GAGCTC SmaI 1529 CCCGGG CCC^GGG 1760 CCCGGG StuI 1484 AGGCCT AGG^CCT 1622 AGGCCT TfiI 102 GATTC G^A[AT]TC 321 GAATC XcmI 194 CCAAGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2072 CCACGAGCTCCATGG XmaCI 1529 CCCGGG C^CCGGG 1760 CCCGGG XmaI 1529 CCCGGG C^CCGGG 1760 CCCGGG XmnI 285 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2017 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII