(Circular) (Six-base) MAPSORT of: pFN21AE3723.seq Check: 4471 from: 1 to: 7039 pFN21AE3723 7039 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4849 278 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4382 1937 Size: 2445 4594 Acc65I G'GTAC_C Cuts at: 4360 4360 Size: 7039 AclI AA'CG_TT Cuts at: 1563 5166 5539 1563 Size: 3603 373 3063 Fragments arranged by size: 3603 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2575 3126 3211 3889 4352 5102 6486 6754 Size: 943 551 85 678 463 750 1384 268 Cuts at: 6754 1632 Size: 1917 Fragments arranged by size: 1917 1384 943 750 678 551 463 268 85 AfeI AGC'GCT Cuts at: 2014 2014 Size: 7039 AflII C'TTAA_G Cuts at: 820 1017 2846 820 Size: 197 1829 5013 Fragments arranged by size: 5013 1829 197 AflIII A'CryG_T Cuts at: 1203 2820 5938 6915 1203 Size: 1617 3118 977 1327 Fragments arranged by size: 3118 1617 1327 977 AgeI A'CCGG_T Cuts at: 5870 5870 Size: 7039 AhdI GACnn_n'nnGTC Cuts at: 3325 5768 3325 Size: 2443 4596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7039 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7039 AlwNI CAG_nnn'CTG Cuts at: 4782 6354 4782 Size: 1572 5467 ApaLI G'TGCA_C Cuts at: 3614 5096 6252 3614 Size: 1482 1156 4401 Fragments arranged by size: 4401 1482 1156 ApoI r'AATT_y Cuts at: 1493 2468 2600 2852 3454 3829 4025 4348 4574 Size: 975 132 252 602 375 196 323 226 Cuts at: 4574 4726 6835 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 975 602 375 323 252 226 196 152 132 AseI AT'TA_AT Cuts at: 160 1032 5593 160 Size: 872 4561 1606 Fragments arranged by size: 4561 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 AvaI C'yCGr_G Cuts at: 1870 1943 2460 2690 3534 3765 4364 1870 Size: 73 517 230 844 231 599 4545 Fragments arranged by size: 4545 844 599 517 231 230 73 AvrII C'CTAG_G Cuts at: 2710 2710 Size: 7039 BamHI G'GATC_C Cuts at: 2741 4369 4736 2741 Size: 1628 367 5044 Fragments arranged by size: 5044 1628 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3358 3664 4283 4360 5815 Size: 325 217 627 1571 306 619 77 1455 Cuts at: 5815 618 Size: 1842 Fragments arranged by size: 1842 1571 1455 627 619 325 306 217 77 BanII G_rGCy'C Cuts at: 1391 3101 3745 4085 4095 4358 1391 Size: 1710 644 340 10 263 4072 Fragments arranged by size: 4072 1710 644 340 263 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3284 3401 4079 928 Size: 971 1385 117 678 3888 Fragments arranged by size: 3888 1385 971 678 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5230 5264 1903 Size: 34 3293 34 3678 Fragments arranged by size: 3678 3293 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4931 6141 4931 Size: 1210 5829 BclI T'GATC_A Cuts at: 1548 1548 Size: 7039 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3545 5650 136 Size: 107 122 71 631 605 1873 2105 1525 Fragments arranged by size: 2105 1873 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7034 7034 Size: 7039 BlpI GC'TnA_GC Cuts at: 4456 4456 Size: 7039 Bme1580I G_kGCm'C Cuts at: 1790 3618 4286 5100 6256 1790 Size: 1828 668 814 1156 2573 Fragments arranged by size: 2573 1828 1156 814 668 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7039 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2238 4770 5728 Size: 416 53 83 399 24 821 2532 958 Cuts at: 5728 442 Size: 1753 Fragments arranged by size: 2532 1753 958 821 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7039 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 7039 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 2270 3404 5699 1239 Size: 1024 7 1134 2295 2579 Fragments arranged by size: 2579 2295 1134 1024 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 2753 5164 6029 6327 2156 Size: 597 2411 865 298 2868 Fragments arranged by size: 2868 2411 865 597 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5702 882 Size: 617 4203 2219 Fragments arranged by size: 4203 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2953 3988 4062 4830 493 Size: 711 1749 1035 74 768 2702 Fragments arranged by size: 2702 1749 1035 768 711 74 BsaBI GATnn'nnATC Cuts at: 7033 7033 Size: 7039 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4846 5228 5863 275 Size: 53 83 186 936 3313 382 635 1451 Fragments arranged by size: 3313 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5472 5870 6144 6291 6702 5472 Size: 398 274 147 411 5809 Fragments arranged by size: 5809 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 3083 3113 4295 Size: 30 165 30 660 30 990 30 1182 Cuts at: 4295 4325 1178 Size: 30 3892 Fragments arranged by size: 3892 1182 990 660 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2266 3329 1186 Size: 973 107 1063 4896 Fragments arranged by size: 4896 1063 973 107 BseYI C'CCAG_C Cuts at: 2087 2870 3674 3687 3797 3850 6242 6738 2087 Size: 783 804 13 110 53 2392 496 2388 Fragments arranged by size: 2392 2388 804 783 496 110 53 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2513 4232 1163 Size: 573 777 1719 3970 Fragments arranged by size: 3970 1719 777 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4489 5250 6278 1540 Size: 275 186 2488 761 1028 2301 Fragments arranged by size: 2488 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3101 3618 4085 5100 5185 6256 3101 Size: 517 467 1015 85 1071 3884 Fragments arranged by size: 3884 1071 1015 517 467 85 BsmI GAATG_Cn' Cuts at: 4550 4643 4550 Size: 93 6946 BsmBI CGTCTCn'nnnn_ Cuts at: 3965 3965 Size: 7039 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3101 3618 3745 4085 4095 4286 4358 Size: 399 1311 517 127 340 10 191 72 Cuts at: 4358 5100 5185 6256 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1311 1071 742 517 399 340 191 127 85 72 10 BspEI T'CCGG_A Cuts at: 6702 6702 Size: 7039 BspHI T'CATG_A Cuts at: 2898 4928 6658 2898 Size: 2030 1730 3279 Fragments arranged by size: 3279 2030 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2553 2850 4394 844 Size: 1709 297 1544 3489 Fragments arranged by size: 3489 1709 1544 297 BsrBI CCG'CTC Cuts at: 1394 1823 3472 4047 4926 1394 Size: 429 1649 575 879 3507 Fragments arranged by size: 3507 1649 879 575 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2602 4305 5534 5708 65 Size: 1183 1354 1703 1229 174 1396 Fragments arranged by size: 1703 1396 1354 1229 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2163 3879 5683 5870 1700 Size: 110 353 1716 1804 187 2869 Fragments arranged by size: 2869 1804 1716 353 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7039 BssSI C'ACGA_G Cuts at: 4078 4199 5099 6111 4078 Size: 121 900 1012 5006 Fragments arranged by size: 5006 1012 900 121 BstAPI GCAn_nnn'nTGC Cuts at: 794 3832 794 Size: 3038 4001 BstBI TT'CG_AA Cuts at: 5843 5843 Size: 7039 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7039 BstYI r'GATC_y Cuts at: 1972 2741 3027 4113 4369 4736 5121 5138 6590 Size: 769 286 1086 256 367 385 17 1452 Cuts at: 6590 6676 6688 7034 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1086 769 385 367 346 286 256 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3312 3312 Size: 7039 BtgI C'CryG_G Cuts at: 513 1065 2826 3006 3543 3570 3645 3668 4086 Size: 552 1761 180 537 27 75 23 418 Cuts at: 4086 4241 4290 513 Size: 155 49 3262 Fragments arranged by size: 3262 1761 552 537 418 180 155 75 49 27 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6300 Fragments arranged by size: 6300 683 56 BtsI GCAGTG_nn' Cuts at: 3739 4003 4220 4555 5349 5369 3739 Size: 264 217 335 794 20 5409 Fragments arranged by size: 5409 794 335 264 217 20 ClaI AT'CG_AT Cuts at: 1554 2908 1554 Size: 1354 5685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7004 DraI TTT'AAA Cuts at: 4343 4343 Size: 7039 DraIII CAC_nnn'GTG Cuts at: 3991 3991 Size: 7039 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6046 809 Size: 507 4730 1802 Fragments arranged by size: 4730 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3069 3204 3484 Size: 54 1425 50 275 18 1239 135 280 Cuts at: 3484 3865 4486 5375 7004 8 Size: 381 621 889 1629 43 Fragments arranged by size: 1629 1425 1239 889 621 381 280 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4486 1537 Size: 275 2674 4090 Fragments arranged by size: 4090 2674 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 3018 4969 1353 Size: 1316 349 1951 3423 Fragments arranged by size: 3423 1951 1316 349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2421 2462 3001 3349 3703 5626 Size: 1064 138 832 41 539 348 354 1923 Cuts at: 5626 6000 6146 387 Size: 374 146 1280 Fragments arranged by size: 1923 1280 1064 832 539 374 354 348 146 138 41 EcoICRI GAG'CTC Cuts at: 3099 4083 3099 Size: 984 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2263 2270 2575 3126 3211 3404 3889 Size: 393 631 7 305 551 85 193 485 Cuts at: 3889 4352 5102 5699 6486 6754 1239 Size: 463 750 597 787 268 1524 Fragments arranged by size: 1524 787 750 631 597 551 485 463 393 305 268 193 85 7 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 7039 EcoO109I rG'GnC_Cy Cuts at: 2977 3531 3888 2977 Size: 554 357 6128 Fragments arranged by size: 6128 554 357 EcoRI G'AATT_C Cuts at: 2600 3454 4348 4726 2600 Size: 854 894 378 4913 Fragments arranged by size: 4913 894 854 378 EcoRV GAT'ATC Cuts at: 4278 4278 Size: 7039 FspI TGC'GCA Cuts at: 5545 5545 Size: 7039 HaeII r_GCGC'y Cuts at: 1678 2016 3168 3226 6186 1678 Size: 338 1152 58 2960 2531 Fragments arranged by size: 2960 2531 1152 338 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2751 2783 3311 3343 5680 Size: 32 135 32 1179 32 528 32 2337 Cuts at: 5680 5712 5754 5786 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2337 1179 528 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4383 4629 669 Size: 988 281 2445 246 3079 Fragments arranged by size: 3079 2445 988 281 246 HindIII A'AGCT_T Cuts at: 748 4334 748 Size: 3586 3453 HpaI GTT'AAC Cuts at: 4629 4629 Size: 7039 KpnI G_GTAC'C Cuts at: 4364 4364 Size: 7039 MfeI C'AATT_G Cuts at: 4638 4638 Size: 7039 MluI A'CGCG_T Cuts at: 6915 6915 Size: 7039 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 3832 6153 6337 1305 Size: 819 1708 2321 184 2007 Fragments arranged by size: 2321 2007 1708 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3071 3867 10 Size: 54 1425 343 1239 796 3182 Fragments arranged by size: 3182 1425 1239 796 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2931 4289 4997 5356 5515 518 Size: 648 1765 1358 708 359 159 2042 Fragments arranged by size: 2042 1765 1358 708 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2739 3146 3220 3572 3670 4140 4243 4455 Size: 1133 407 74 352 98 470 103 212 Cuts at: 4455 5132 6280 6525 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 677 470 407 352 245 212 103 98 74 NaeI GCC'GGC Cuts at: 3881 3881 Size: 7039 NcoI C'CATG_G Cuts at: 513 1065 3006 4086 4290 513 Size: 552 1941 1080 204 3262 Fragments arranged by size: 3262 1941 1080 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 7039 NgoMIV G'CCGG_C Cuts at: 3879 3879 Size: 7039 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7039 NotI GC'GGCC_GC Cuts at: 4486 4486 Size: 7039 NspI r_CATG'y Cuts at: 1132 1229 2824 4171 4183 4397 5942 1132 Size: 97 1595 1347 12 214 1545 2229 Fragments arranged by size: 2229 1595 1545 1347 214 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7039 PciI A'CATG_T Cuts at: 2820 5938 2820 Size: 3118 3921 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7039 PfoI T'CCnGG_A Cuts at: 3030 3030 Size: 7039 PmeI GTTT'AAAC Cuts at: 4343 4343 Size: 7039 PmlI CAC'GTG Cuts at: 3988 4062 3988 Size: 74 6965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5108 5140 6621 6653 5108 Size: 32 1481 32 5494 Fragments arranged by size: 5494 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2977 3531 2977 Size: 554 6485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7039 PsiI TTA'TAA Cuts at: 4609 4609 Size: 7039 PspXI vC'TCGA_Gb Cuts at: 2460 2460 Size: 7039 PstI C_TGCA'G Cuts at: 830 4391 830 Size: 3561 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7039 PvuII CAG'CTG Cuts at: 3146 4140 3146 Size: 994 6045 SacI G_AGCT'C Cuts at: 3101 4085 3101 Size: 984 6055 SacII CC_GC'GG Cuts at: 3573 3671 4244 3573 Size: 98 573 6368 Fragments arranged by size: 6368 573 98 SalI G'TCGA_C Cuts at: 1936 4381 1936 Size: 2445 4594 SanDI GG'GwC_CC Cuts at: 3531 3531 Size: 7039 SbfI CC_TGCA'GG Cuts at: 4391 4391 Size: 7039 ScaI AGT'ACT Cuts at: 2498 5287 6999 2498 Size: 2789 1712 2538 Fragments arranged by size: 2789 2538 1712 SfcI C'TryA_G Cuts at: 826 1045 4387 5522 6203 6394 826 Size: 219 3342 1135 681 191 1471 Fragments arranged by size: 3342 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7039 SmaI CCC'GGG Cuts at: 1872 3536 3767 4366 1872 Size: 1664 231 599 4545 Fragments arranged by size: 4545 1664 599 231 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 2460 2768 2846 5143 6044 Size: 197 926 228 289 308 78 2297 901 Cuts at: 6044 6306 820 Size: 262 1553 Fragments arranged by size: 2297 1553 926 901 308 289 262 228 197 78 SnaBI TAC'GTA Cuts at: 493 4830 493 Size: 4337 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7039 SphI G_CATG'C Cuts at: 1132 4183 4397 1132 Size: 3051 214 3774 Fragments arranged by size: 3774 3051 214 SspI AAT'ATT Cuts at: 5 52 4963 5 Size: 47 4911 2081 Fragments arranged by size: 4911 2081 47 StuI AGG'CCT Cuts at: 1517 3491 3629 1517 Size: 1974 138 4927 Fragments arranged by size: 4927 1974 138 StyI C'CwwG_G Cuts at: 513 1065 2064 2199 2710 3006 3354 4086 4290 Size: 552 999 135 511 296 348 732 204 Cuts at: 4290 4478 513 Size: 188 3074 Fragments arranged by size: 3074 999 732 552 511 348 296 204 188 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3765 3978 5066 5083 5236 5421 5866 Size: 1255 2185 213 1088 17 153 185 445 Cuts at: 5866 325 Size: 1498 Fragments arranged by size: 2185 1498 1255 1088 445 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4164 5285 Size: 274 80 33 51 1443 519 1668 1121 Cuts at: 5285 6997 96 Size: 1712 138 Fragments arranged by size: 1712 1668 1443 1121 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 2679 2711 3581 3613 6999 7031 2063 Size: 32 584 32 870 32 3386 32 2071 Fragments arranged by size: 3386 2071 870 584 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7039 XbaI T'CTAG_A Cuts at: 4375 4375 Size: 7039 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 4084 2206 Size: 1878 5161 XhoI C'TCGA_G Cuts at: 1943 2460 1943 Size: 517 6522 XmaI C'CCGG_G Cuts at: 1870 3534 3765 4364 1870 Size: 1664 231 599 4545 Fragments arranged by size: 4545 1664 599 231 XmnI GAAnn'nnTTC Cuts at: 2294 4026 5168 2294 Size: 1732 1142 4165 Fragments arranged by size: 4165 1732 1142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4847 276 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI