(Linear) (Six-base) MAPSORT of: KIEE3723.seq Check: 495 from: 1 to: 2334 KIEE3723.orf With 182 enzymes: SgfI * July 3, 2012 18:18 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 570 1121 1206 1884 2334 Size: 570 551 85 678 450 Fragments arranged by size: 678 570 551 450 85 AfeI AGC'GCT Cuts at: 0 9 2334 Size: 9 2325 AflII C'TTAA_G Cuts at: 0 841 2334 Size: 841 1493 AflIII A'CryG_T Cuts at: 0 815 2334 Size: 815 1519 AhdI GACnn_n'nnGTC Cuts at: 0 1320 2334 Size: 1320 1014 ApaLI G'TGCA_C Cuts at: 0 1609 2334 Size: 1609 725 ApoI r'AATT_y Cuts at: 0 463 595 847 1449 1824 2020 2334 Size: 463 132 252 602 375 196 314 Fragments arranged by size: 602 463 375 314 252 196 132 AvaI C'yCGr_G Cuts at: 0 455 685 1529 1760 2334 Size: 455 230 844 231 574 Fragments arranged by size: 844 574 455 231 230 AvrII C'CTAG_G Cuts at: 0 705 2334 Size: 705 1629 BamHI G'GATC_C Cuts at: 0 736 2334 Size: 736 1598 BanI G'GyrC_C Cuts at: 0 1353 1659 2278 2334 Size: 1353 306 619 56 Fragments arranged by size: 1353 619 306 56 BanII G_rGCy'C Cuts at: 0 1096 1740 2080 2090 2334 Size: 1096 644 340 10 244 Fragments arranged by size: 1096 644 340 244 10 BbsI GAAGACnn'nnnn_ Cuts at: 0 1279 1396 2074 2334 Size: 1279 117 678 260 Fragments arranged by size: 1279 678 260 117 BglI GCCn_nnn'nGGC Cuts at: 0 1540 2334 Size: 1540 794 Bme1580I G_kGCm'C Cuts at: 0 1613 2281 2334 Size: 1613 668 53 Fragments arranged by size: 1613 668 53 BmrI ACTGGGnnnn_n' Cuts at: 0 233 2334 Size: 233 2101 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 733 2334 Size: 733 1601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 265 1399 2334 Size: 258 7 1134 935 Fragments arranged by size: 1134 935 258 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 748 2334 Size: 151 597 1586 Fragments arranged by size: 1586 597 151 BsaAI yAC'GTr Cuts at: 0 948 1983 2057 2334 Size: 948 1035 74 277 Fragments arranged by size: 1035 948 277 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 58 88 1078 1108 2290 2320 2334 Size: 58 30 990 30 1182 30 14 Fragments arranged by size: 1182 990 58 30 30 30 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 261 1324 2334 Size: 154 107 1063 1010 Fragments arranged by size: 1063 1010 154 107 BseYI C'CCAG_C Cuts at: 0 82 865 1669 1682 1792 1845 2334 Size: 82 783 804 13 110 53 489 Fragments arranged by size: 804 783 489 110 82 53 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 2227 2334 Size: 508 1719 107 Fragments arranged by size: 1719 508 107 BsiHKAI G_wGCw'C Cuts at: 0 1096 1613 2080 2334 Size: 1096 517 467 254 Fragments arranged by size: 1096 517 467 254 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1960 2334 Size: 1960 374 Bsp1286I G_dGCh'C Cuts at: 0 1096 1613 1740 2080 2090 2281 2334 Size: 1096 517 127 340 10 191 53 Fragments arranged by size: 1096 517 340 191 127 53 10 BspHI T'CATG_A Cuts at: 0 893 2334 Size: 893 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 548 845 2334 Size: 548 297 1489 Fragments arranged by size: 1489 548 297 BsrBI CCG'CTC Cuts at: 0 1467 2042 2334 Size: 1467 575 292 Fragments arranged by size: 1467 575 292 BsrDI GCAATG_nn' Cuts at: 0 597 2300 2334 Size: 597 1703 34 Fragments arranged by size: 1703 597 34 BsrFI r'CCGG_y Cuts at: 0 158 1874 2334 Size: 158 1716 460 Fragments arranged by size: 1716 460 158 BssSI C'ACGA_G Cuts at: 0 2073 2194 2334 Size: 2073 121 140 Fragments arranged by size: 2073 140 121 BstAPI GCAn_nnn'nTGC Cuts at: 0 1827 2334 Size: 1827 507 BstYI r'GATC_y Cuts at: 0 736 1022 2108 2334 Size: 736 286 1086 226 Fragments arranged by size: 1086 736 286 226 Bsu36I CC'TnA_GG Cuts at: 0 1307 2334 Size: 1307 1027 BtgI C'CryG_G Cuts at: 0 821 1001 1538 1565 1640 1663 2081 2236 Size: 821 180 537 27 75 23 418 155 Cuts at: 2236 2285 2334 Size: 49 49 Fragments arranged by size: 821 537 418 180 155 75 49 49 27 23 BtsI GCAGTG_nn' Cuts at: 0 1734 1998 2215 2334 Size: 1734 264 217 119 Fragments arranged by size: 1734 264 217 119 ClaI AT'CG_AT Cuts at: 0 903 2334 Size: 903 1431 DraIII CAC_nnn'GTG Cuts at: 0 1986 2334 Size: 1986 348 EaeI y'GGCC_r Cuts at: 0 1064 1199 1479 1860 2334 Size: 1064 135 280 381 474 Fragments arranged by size: 1064 474 381 280 135 EarI CTCTTCn'nnn_ Cuts at: 0 664 1013 2334 Size: 664 349 1321 Fragments arranged by size: 1321 664 349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 416 457 996 1344 1698 2334 Size: 416 41 539 348 354 636 Fragments arranged by size: 636 539 416 354 348 41 EcoICRI GAG'CTC Cuts at: 0 1094 2078 2334 Size: 1094 984 256 Fragments arranged by size: 1094 984 256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 265 570 1121 1206 1399 1884 2334 Size: 258 7 305 551 85 193 485 450 Fragments arranged by size: 551 485 450 305 258 193 85 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1480 2334 Size: 1480 854 EcoO109I rG'GnC_Cy Cuts at: 0 972 1526 1883 2334 Size: 972 554 357 451 Fragments arranged by size: 972 554 451 357 EcoRI G'AATT_C Cuts at: 0 595 1449 2334 Size: 595 854 885 Fragments arranged by size: 885 854 595 EcoRV GAT'ATC Cuts at: 0 2273 2334 Size: 2273 61 HaeII r_GCGC'y Cuts at: 0 11 1163 1221 2334 Size: 11 1152 58 1113 Fragments arranged by size: 1152 1113 58 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 746 778 1306 1338 2334 Size: 746 32 528 32 996 Fragments arranged by size: 996 746 528 32 32 HindIII A'AGCT_T Cuts at: 0 2329 2334 Size: 2329 5 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 119 1827 2334 Size: 119 1708 507 Fragments arranged by size: 1708 507 119 MscI TGG'CCA Cuts at: 0 1066 1862 2334 Size: 1066 796 472 Fragments arranged by size: 1066 796 472 MslI CAynn'nnrTG Cuts at: 0 926 2284 2334 Size: 926 1358 50 Fragments arranged by size: 1358 926 50 MspA1I CmG'CkG Cuts at: 0 734 1141 1215 1567 1665 2135 2238 2334 Size: 734 407 74 352 98 470 103 96 Fragments arranged by size: 734 470 407 352 103 98 96 74 NaeI GCC'GGC Cuts at: 0 1876 2334 Size: 1876 458 NcoI C'CATG_G Cuts at: 0 1001 2081 2285 2334 Size: 1001 1080 204 49 Fragments arranged by size: 1080 1001 204 49 NgoMIV G'CCGG_C Cuts at: 0 1874 2334 Size: 1874 460 NspI r_CATG'y Cuts at: 0 819 2166 2178 2334 Size: 819 1347 12 156 Fragments arranged by size: 1347 819 156 12 PciI A'CATG_T Cuts at: 0 815 2334 Size: 815 1519 PfoI T'CCnGG_A Cuts at: 0 1025 2334 Size: 1025 1309 PmlI CAC'GTG Cuts at: 0 1983 2057 2334 Size: 1983 74 277 Fragments arranged by size: 1983 277 74 PpuMI rG'GwC_Cy Cuts at: 0 972 1526 2334 Size: 972 554 808 Fragments arranged by size: 972 808 554 PspXI vC'TCGA_Gb Cuts at: 0 455 2334 Size: 455 1879 PvuII CAG'CTG Cuts at: 0 1141 2135 2334 Size: 1141 994 199 Fragments arranged by size: 1141 994 199 SacI G_AGCT'C Cuts at: 0 1096 2080 2334 Size: 1096 984 254 Fragments arranged by size: 1096 984 254 SacII CC_GC'GG Cuts at: 0 1568 1666 2239 2334 Size: 1568 98 573 95 Fragments arranged by size: 1568 573 98 95 SanDI GG'GwC_CC Cuts at: 0 1526 2334 Size: 1526 808 ScaI AGT'ACT Cuts at: 0 493 2334 Size: 493 1841 SmaI CCC'GGG Cuts at: 0 1531 1762 2334 Size: 1531 231 572 Fragments arranged by size: 1531 572 231 SmlI C'TyrA_G Cuts at: 0 166 455 763 841 2334 Size: 166 289 308 78 1493 Fragments arranged by size: 1493 308 289 166 78 SphI G_CATG'C Cuts at: 0 2178 2334 Size: 2178 156 StuI AGG'CCT Cuts at: 0 1486 1624 2334 Size: 1486 138 710 Fragments arranged by size: 1486 710 138 StyI C'CwwG_G Cuts at: 0 59 194 705 1001 1349 2081 2285 2334 Size: 59 135 511 296 348 732 204 49 Fragments arranged by size: 732 511 348 296 204 135 59 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1760 1973 2334 Size: 1760 213 361 Fragments arranged by size: 1760 361 213 TatI w'GTAC_w Cuts at: 0 491 2159 2334 Size: 491 1668 175 Fragments arranged by size: 1668 491 175 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 58 90 674 706 1576 1608 2334 Size: 58 32 584 32 870 32 726 Fragments arranged by size: 870 726 584 58 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 201 2079 2334 Size: 201 1878 255 Fragments arranged by size: 1878 255 201 XhoI C'TCGA_G Cuts at: 0 455 2334 Size: 455 1879 XmaI C'CCGG_G Cuts at: 0 1529 1760 2334 Size: 1529 231 574 Fragments arranged by size: 1529 574 231 XmnI GAAnn'nnTTC Cuts at: 0 289 2021 2334 Size: 289 1732 313 Fragments arranged by size: 1732 313 289 Enzymes that do cut: AcuI AfeI AflII AflIII AhdI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BglI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PfoI PmlI PpuMI PspXI PvuII SacI SacII SanDI ScaI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DrdI EagI FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SalI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI