################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BalI BbvCI BcgI BclI BfrI BglII BlnI BsaBI BsaI BseRI BsgI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SbfI SfiI SfuI SgrAI SnaBI Sse8387I SspBI StuI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1730 GTCGAC GT^[AC][GT]AC AccIII 972 TCCGGA T^CCGGA AcyI 16 GGCGCC G[GA]^CG[CT]C AseI 2116 ATTAAT AT^TAAT AsnI 2116 ATTAAT AT^TAAT AspI 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 539 GGATCC G^GATCC BanI 16 GGCGCC G^G[CT][GA]CC BbeI 16 GGCGCC GGCGC^C BbrPI 1106 CACGTG CAC^GTG BglI 562 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 67 GCTGAGC GC^T[ACGT]AGC Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC BsaHI 16 GGCGCC G[GA]^CG[CT]C BsaWI 972 TCCGGA [AT]^CCGG[AT] BseAI 972 TCCGGA T^CCGGA BsmBI 2595 CGTCTC CGTCTC(1/5) BsmI 2285 GAATGC GAATGC(1/-1) BspEI 972 TCCGGA T^CCGGA BspMI 1830 ACCTGC ACCTGC(4/8) BssHII 176 GCGCGC G^CGCGC BstAPI 736 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 67 GCTGAGC GC^T[ACGT]AGC EaeI 83 CGGCCA [CT]^GGCC[GA] Hin1I 16 GGCGCC G[GA]^CG[CT]C HindIII 1430 AAGCTT A^AGCTT HpaI 1338 GTTAAC GTT^AAC KasI 16 GGCGCC G^GCGCC MroI 972 TCCGGA T^CCGGA NarI 16 GGCGCC GG^CGCC PaeR7I 1381 CTCGAG C^TCGAG PflFI 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1106 CACGTG CAC^GTG PmlI 1106 CACGTG CAC^GTG PshBI 2116 ATTAAT AT^TAAT SacI 1400 GAGCTC GAGCT^C SalI 1730 GTCGAC G^TCGAC SapI 1402 GCTCTTC GCTCTTC(1/4) ScaI 1189 AGTACT AGT^ACT SexAI 1496 ACCTGGT A^CC[AT]GGT SfoI 16 GGCGCC GGC^GCC SmlI 1381 CTCGAG C^T[CT][GA]AG SpeI 1096 ACTAGT A^CTAGT SphI 2868 GCATGC GCATG^C SspI 2959 AATATT AAT^ATT SwaI 2312 ATTTAAAT ATTT^AAAT Tth111I 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2433 TCTAGA T^CTAGA XcmI 914 CCACTGGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1381 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 177 CGCG CG^CG 184 CGCG AfaI 3 GTAC GT^AC 1190 GTAC AflIII 1105 ACACGT A^C[GA][CT]GT 1375 ACGTGT Alw44I 692 GTGCAC G^TGCAC 907 GTGCAC ApaLI 692 GTGCAC G^TGCAC 907 GTGCAC BanII 569 GGGCTC G[GA]GC[CT]^C 1400 GAGCTC BsaAI 1106 CACGTG [CT]AC^GT[GA] 1374 TACGTG BssSI 135 CTCGTG CACGAG(-5/-1) 235 CTCGTG BstUI 177 CGCG CG^CG 184 CGCG BstXI 1295 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2531 CCATACCTGTGG BstYI 539 GGATCC [GA]^GATC[CT] 2525 GGATCT DsaI 468 CCATGG C^C[GA][CT]GG 1154 CCATGG EcoRI 331 GAATTC G^AATTC 2739 GAATTC EcoT22I 1471 ATGCAT ATGCA^T 1963 ATGCAT HgaI 167 GACGC GACGC(5/10) 2058 GCGTC MflI 539 GGATCC [GA]^GATC[CT] 2525 GGATCT MvnI 177 CGCG CG^CG 184 CGCG NcoI 468 CCATGG C^CATGG 1154 CCATGG NdeI 902 CATATG CA^TATG 1064 CATATG NsiI 1471 ATGCAT ATGCA^T 1963 ATGCAT PpuMI 128 GGGTCCT [GA]G^G[AT]CC[CT] 526 AGGTCCC RsaI 3 GTAC GT^AC 1190 GTAC SmaI 61 CCCGGG CCC^GGG 1114 CCCGGG TaqI 1382 TCGA T^CGA 1731 TCGA Tsp45I 950 GTGAC ^GT[GC]AC 1078 GTGAC TthHB8I 1382 TCGA T^CGA 1731 TCGA XhoII 539 GGATCC [GA]^GATC[CT] 2525 GGATCT XmaCI 61 CCCGGG C^CCGGG 1114 CCCGGG XmaI 61 CCCGGG C^CCGGG 1114 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I XmnI