(Circular) (Six-base) MAPSORT of: pFN21AE4255.seq Check: 2129 from: 1 to: 7771 pFN21AE4255 7771 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5581 278 Size: 53 83 186 936 4045 2468 Fragments arranged by size: 4045 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3736 5114 1937 Size: 1799 1378 4594 Fragments arranged by size: 4594 1799 1378 Acc65I G'GTAC_C Cuts at: 5092 5092 Size: 7771 AclI AA'CG_TT Cuts at: 1563 5898 6271 1563 Size: 4335 373 3063 Fragments arranged by size: 4335 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 2723 3045 4101 4704 5834 7218 7486 Size: 702 389 322 1056 603 1130 1384 268 Cuts at: 7486 1632 Size: 1917 Fragments arranged by size: 1917 1384 1130 1056 702 603 389 322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7574 AflIII A'CryG_T Cuts at: 1203 3110 3380 6670 7647 1203 Size: 1907 270 3290 977 1327 Fragments arranged by size: 3290 1907 1327 977 270 AgeI A'CCGG_T Cuts at: 6602 6602 Size: 7771 AhdI GACnn_n'nnGTC Cuts at: 6500 6500 Size: 7771 AleI CACnn'nnGTG Cuts at: 1166 2750 1166 Size: 1584 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7771 AlwNI CAG_nnn'CTG Cuts at: 5514 7086 5514 Size: 1572 6199 ApaLI G'TGCA_C Cuts at: 2697 2912 5828 6984 2697 Size: 215 2916 1156 3484 Fragments arranged by size: 3484 2916 1156 215 ApoI r'AATT_y Cuts at: 1493 2336 3998 4115 4128 4625 4744 4838 5080 Size: 843 1662 117 13 497 119 94 242 Cuts at: 5080 5306 5458 7567 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1662 843 497 242 226 152 119 117 94 13 AseI AT'TA_AT Cuts at: 160 1032 4122 6325 160 Size: 872 3090 2203 1606 Fragments arranged by size: 3090 2203 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7771 AvaI C'yCGr_G Cuts at: 1870 1943 2066 3119 3386 5096 1870 Size: 73 123 1053 267 1710 4545 Fragments arranged by size: 4545 1710 1053 267 123 73 BamHI G'GATC_C Cuts at: 2544 5101 5468 2544 Size: 2557 367 4847 Fragments arranged by size: 4847 2557 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 5092 6547 618 Size: 325 217 627 234 3071 1455 1842 Fragments arranged by size: 3071 1842 1455 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2578 3409 5090 1391 Size: 1187 831 1681 4072 Fragments arranged by size: 4072 1681 1187 831 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 7771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2284 3033 3811 928 Size: 971 385 749 778 4888 Fragments arranged by size: 4888 971 778 749 385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5962 5996 1903 Size: 34 4025 34 3678 Fragments arranged by size: 4025 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2804 3091 4227 4757 5663 6873 2804 Size: 287 1136 530 906 1210 3702 Fragments arranged by size: 3702 1210 1136 906 530 287 BclI T'GATC_A Cuts at: 1548 1548 Size: 7771 BfrBI ATG'CAT Cuts at: 3478 3970 3478 Size: 492 7279 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 6382 136 Size: 107 122 71 631 605 901 3809 1525 Fragments arranged by size: 3809 1525 901 631 605 122 107 71 BglII A'GATC_T Cuts at: 7766 7766 Size: 7771 BlpI GC'TnA_GC Cuts at: 2073 5188 2073 Size: 3115 4656 Bme1580I G_kGCm'C Cuts at: 1790 2701 2916 5832 6988 1790 Size: 911 215 2916 1156 2573 Fragments arranged by size: 2916 2573 1156 911 215 BmgBI CAC'GTC Cuts at: 1318 4862 1318 Size: 3544 4227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2660 3843 4504 Size: 416 53 83 399 24 1243 1183 661 Cuts at: 4504 5502 6460 442 Size: 998 958 1753 Fragments arranged by size: 1753 1243 1183 998 958 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7771 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 2521 4487 2122 Size: 399 1966 5406 Fragments arranged by size: 5406 1966 399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2014 2558 3306 4164 6431 1239 Size: 775 544 748 858 2267 2579 Fragments arranged by size: 2579 2267 858 775 748 544 Bpu10I CC'TnA_GC Cuts at: 2556 2556 Size: 7771 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5896 6761 7059 5896 Size: 865 298 6608 Fragments arranged by size: 6608 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6434 882 Size: 617 4935 2219 Fragments arranged by size: 4935 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3113 3381 5562 493 Size: 711 1909 268 2181 2702 Fragments arranged by size: 2702 2181 1909 711 268 BsaBI GATnn'nnATC Cuts at: 7765 7765 Size: 7771 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 5578 5960 6595 Size: 53 83 186 936 489 3556 382 635 Cuts at: 6595 275 Size: 1451 Fragments arranged by size: 3556 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2977 6204 6602 6876 7023 7434 2977 Size: 3227 398 274 147 411 3314 Fragments arranged by size: 3314 3227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 3100 3130 1178 Size: 30 165 30 1002 30 665 30 5819 Fragments arranged by size: 5819 1002 665 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7771 BseYI C'CCAG_C Cuts at: 2167 2323 2482 2616 2806 6974 7470 2167 Size: 156 159 134 190 4168 496 2468 Fragments arranged by size: 4168 2468 496 190 159 156 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5221 5982 7010 1540 Size: 275 186 3220 761 1028 2301 Fragments arranged by size: 3220 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2701 2916 3118 3409 5832 5917 6988 2262 Size: 439 215 202 291 2423 85 1071 3045 Fragments arranged by size: 3045 2423 1071 439 291 215 202 85 BsmI GAATG_Cn' Cuts at: 4296 5282 5375 4296 Size: 986 93 6692 Fragments arranged by size: 6692 986 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4606 4606 Size: 7771 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2578 2701 2916 3118 3409 5090 Size: 399 472 316 123 215 202 291 1681 Cuts at: 5090 5832 5917 6988 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1681 1071 742 472 399 316 291 215 202 123 85 BspEI T'CCGG_A Cuts at: 2977 7434 2977 Size: 4457 3314 BspHI T'CATG_A Cuts at: 5660 7390 5660 Size: 1730 6041 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3844 5126 844 Size: 3000 1282 3489 Fragments arranged by size: 3489 3000 1282 BsrBI CCG'CTC Cuts at: 1394 1823 5658 1394 Size: 429 3835 3507 Fragments arranged by size: 3835 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6266 6440 65 Size: 1183 5018 174 1396 Fragments arranged by size: 5018 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6415 6602 1700 Size: 110 4605 187 2869 Fragments arranged by size: 4605 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7771 BssHII G'CGCG_C Cuts at: 1925 2181 1925 Size: 256 7515 BssSI C'ACGA_G Cuts at: 2140 2240 5831 6843 2140 Size: 100 3591 1012 3068 Fragments arranged by size: 3591 3068 1012 100 BstAPI GCAn_nnn'nTGC Cuts at: 794 2747 794 Size: 1953 5818 BstBI TT'CG_AA Cuts at: 6575 6575 Size: 7771 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7771 BstXI CCAn_nnnn'nTGG Cuts at: 3307 4543 3307 Size: 1236 6535 BstYI r'GATC_y Cuts at: 1972 2544 4530 5101 5468 5853 5870 7322 7408 Size: 572 1986 571 367 385 17 1452 86 Cuts at: 7408 7420 7766 1972 Size: 12 346 1977 Fragments arranged by size: 1986 1977 1452 572 571 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2473 3159 513 Size: 552 1408 686 5125 Fragments arranged by size: 5125 1408 686 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7032 Fragments arranged by size: 7032 683 56 BtsI GCAGTG_nn' Cuts at: 2745 3186 5287 6081 6101 2745 Size: 441 2101 794 20 4415 Fragments arranged by size: 4415 2101 794 441 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7771 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7736 DraI TTT'AAA Cuts at: 3270 4320 4395 5075 3270 Size: 1050 75 680 5966 Fragments arranged by size: 5966 1050 680 75 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6778 809 Size: 507 5462 1802 Fragments arranged by size: 5462 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 5218 6107 Size: 54 1425 50 275 18 258 3130 889 Cuts at: 6107 7736 8 Size: 1629 43 Fragments arranged by size: 3130 1629 1425 889 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5218 1537 Size: 275 3406 4090 Fragments arranged by size: 4090 3406 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 3414 4176 4415 5701 1353 Size: 1472 589 762 239 1286 3423 Fragments arranged by size: 3423 1472 1286 762 589 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6358 6732 6878 387 Size: 1064 138 4769 374 146 1280 Fragments arranged by size: 4769 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3407 3407 Size: 7771 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2014 2334 2558 2723 3045 3306 4101 Size: 393 382 320 224 165 322 261 795 Cuts at: 4101 4164 4704 5834 6431 7218 7486 1239 Size: 63 540 1130 597 787 268 1524 Fragments arranged by size: 1524 1130 795 787 597 540 393 382 322 320 268 261 224 165 63 EcoO109I rG'GnC_Cy Cuts at: 2134 2532 2983 2134 Size: 398 451 6922 Fragments arranged by size: 6922 451 398 EcoRI G'AATT_C Cuts at: 2336 4744 5080 5458 2336 Size: 2408 336 378 4649 Fragments arranged by size: 4649 2408 378 336 FspI TGC'GCA Cuts at: 6277 6277 Size: 7771 HaeII r_GCGC'y Cuts at: 1678 2025 2047 2104 6918 1678 Size: 347 22 57 4814 2531 Fragments arranged by size: 4814 2531 347 57 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2780 2812 3076 3108 4266 Size: 32 135 32 1208 32 264 32 1158 Cuts at: 4266 4298 5031 5063 6412 6444 6486 6518 1373 Size: 32 733 32 1349 32 42 32 2626 Fragments arranged by size: 2626 1349 1208 1158 733 264 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3737 4513 5115 5361 669 Size: 988 281 1407 392 776 602 246 3079 Fragments arranged by size: 3079 1407 988 776 602 392 281 246 HindIII A'AGCT_T Cuts at: 748 3435 748 Size: 2687 5084 HpaI GTT'AAC Cuts at: 3345 5361 3345 Size: 2016 5755 KasI G'GCGC_C Cuts at: 2021 2021 Size: 7771 KpnI G_GTAC'C Cuts at: 5096 5096 Size: 7771 MfeI C'AATT_G Cuts at: 5370 5370 Size: 7771 MluI A'CGCG_T Cuts at: 7647 7647 Size: 7771 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3798 6885 7069 1305 Size: 2493 3087 184 2007 Fragments arranged by size: 3087 2493 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5949 Fragments arranged by size: 5949 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2750 3305 3675 3965 4541 5729 6088 Size: 648 1584 555 370 290 576 1188 359 Cuts at: 6088 6247 518 Size: 159 2042 Fragments arranged by size: 2042 1584 1188 648 576 555 370 359 290 159 MspA1I CmG'CkG Cuts at: 1606 2094 2167 2323 2482 2641 2730 2806 3604 Size: 488 73 156 159 159 89 76 798 Cuts at: 3604 3640 5187 5864 7012 7257 1606 Size: 36 1547 677 1148 245 2120 Fragments arranged by size: 2120 1547 1148 798 677 488 245 159 159 156 89 76 73 36 NarI GG'CG_CC Cuts at: 2022 2022 Size: 7771 NcoI C'CATG_G Cuts at: 513 1065 2473 3159 513 Size: 552 1408 686 5125 Fragments arranged by size: 5125 1408 686 552 NdeI CA'TA_TG Cuts at: 387 2908 3070 387 Size: 2521 162 5088 Fragments arranged by size: 5088 2521 162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7771 NotI GC'GGCC_GC Cuts at: 5218 5218 Size: 7771 NsiI A_TGCA'T Cuts at: 3480 3972 3480 Size: 492 7279 NspI r_CATG'y Cuts at: 1132 1229 3964 4240 4877 5129 6674 1132 Size: 97 2735 276 637 252 1545 2229 Fragments arranged by size: 2735 2229 1545 637 276 252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7771 PciI A'CATG_T Cuts at: 6670 6670 Size: 7771 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 3160 4202 1108 Size: 1317 735 1042 4677 Fragments arranged by size: 4677 1317 1042 735 PmeI GTTT'AAAC Cuts at: 5075 5075 Size: 7771 PmlI CAC'GTG Cuts at: 3113 3113 Size: 7771 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5840 5872 7353 7385 5840 Size: 32 1481 32 6226 Fragments arranged by size: 6226 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2532 2134 Size: 398 7373 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7771 PsiI TTA'TAA Cuts at: 5341 5341 Size: 7771 PstI C_TGCA'G Cuts at: 830 2043 2085 3841 5123 830 Size: 1213 42 1756 1282 3478 Fragments arranged by size: 3478 1756 1282 1213 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7771 PvuII CAG'CTG Cuts at: 2167 2323 2482 2641 2730 2806 3640 2167 Size: 156 159 159 89 76 834 6298 Fragments arranged by size: 6298 834 159 159 156 89 76 SacI G_AGCT'C Cuts at: 3409 3409 Size: 7771 SalI G'TCGA_C Cuts at: 1936 3735 5113 1936 Size: 1799 1378 4594 Fragments arranged by size: 4594 1799 1378 SapI GCTCTTCn'nnn_ Cuts at: 3414 3414 Size: 7771 SbfI CC_TGCA'GG Cuts at: 5123 5123 Size: 7771 ScaI AGT'ACT Cuts at: 3196 6019 7731 3196 Size: 2823 1712 3236 Fragments arranged by size: 3236 2823 1712 SexAI A'CCwGG_T Cuts at: 3501 3501 Size: 7771 SfcI C'TryA_G Cuts at: 826 1045 2039 2081 3837 5119 6254 6935 7126 Size: 219 994 42 1756 1282 1135 681 191 Cuts at: 7126 826 Size: 1471 Fragments arranged by size: 1756 1471 1282 1135 994 681 219 191 42 SfoI GGC'GCC Cuts at: 2023 2023 Size: 7771 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7771 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7771 SmaI CCC'GGG Cuts at: 1872 2068 3121 5098 1872 Size: 196 1053 1977 4545 Fragments arranged by size: 4545 1977 1053 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 3386 5875 6776 7038 820 Size: 197 926 1443 2489 901 262 1553 Fragments arranged by size: 2489 1553 1443 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5562 493 Size: 5069 2702 SpeI A'CTAG_T Cuts at: 152 3101 152 Size: 2949 4822 SphI G_CATG'C Cuts at: 1132 4877 5129 1132 Size: 3745 252 3774 Fragments arranged by size: 3774 3745 252 SspI AAT'ATT Cuts at: 5 52 4966 5695 5 Size: 47 4914 729 2081 Fragments arranged by size: 4914 2081 729 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7771 StyI C'CwwG_G Cuts at: 513 1065 2473 3159 4821 5210 513 Size: 552 1408 686 1662 389 3074 Fragments arranged by size: 3074 1662 1408 686 552 389 SwaI ATTT'AAAT Cuts at: 4320 4320 Size: 7771 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4027 4956 5068 5798 5815 5968 6153 Size: 1255 2447 929 112 730 17 153 185 Cuts at: 6153 6598 325 Size: 445 1498 Fragments arranged by size: 2447 1498 1255 929 730 445 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3194 6017 Size: 274 80 33 51 1443 30 1187 2823 Cuts at: 6017 7729 96 Size: 1712 138 Fragments arranged by size: 2823 1712 1443 1187 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 3100 3132 7731 7763 2405 Size: 32 663 32 4599 32 2413 Fragments arranged by size: 4599 2413 663 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4038 1316 Size: 2722 5049 XbaI T'CTAG_A Cuts at: 4438 5107 4438 Size: 669 7102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2926 2926 Size: 7771 XhoI C'TCGA_G Cuts at: 1943 3386 1943 Size: 1443 6328 XmaI C'CCGG_G Cuts at: 1870 2066 3119 5096 1870 Size: 196 1053 1977 4545 Fragments arranged by size: 4545 1977 1053 196 XmnI GAAnn'nnTTC Cuts at: 2208 2498 2774 3978 4805 4838 5013 5900 2208 Size: 290 276 1204 827 33 175 887 4079 Fragments arranged by size: 4079 1204 887 827 290 276 175 33 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5579 276 Size: 53 83 186 936 4045 2468 Fragments arranged by size: 4045 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI