(Linear) (Six-base) MAPSORT of: KIEE4255.seq Check: 2513 from: 1 to: 3066 KIEE4255.orf With 182 enzymes: SgfI * July 3, 2012 18:51 .. AccI GT'mk_AC Cuts at: 0 1731 3066 Size: 1731 1335 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 718 1040 2096 2699 3066 Size: 329 389 322 1056 603 367 Fragments arranged by size: 1056 603 389 367 329 322 AflIII A'CryG_T Cuts at: 0 1105 1375 3066 Size: 1105 270 1691 Fragments arranged by size: 1691 1105 270 AleI CACnn'nnGTG Cuts at: 0 745 3066 Size: 745 2321 ApaLI G'TGCA_C Cuts at: 0 692 907 3066 Size: 692 215 2159 Fragments arranged by size: 2159 692 215 ApoI r'AATT_y Cuts at: 0 331 1993 2110 2123 2620 2739 2833 3066 Size: 331 1662 117 13 497 119 94 233 Fragments arranged by size: 1662 497 331 233 119 117 94 13 AseI AT'TA_AT Cuts at: 0 2117 3066 Size: 2117 949 AvaI C'yCGr_G Cuts at: 0 61 1114 1381 3066 Size: 61 1053 267 1685 Fragments arranged by size: 1685 1053 267 61 BamHI G'GATC_C Cuts at: 0 539 3066 Size: 539 2527 BanI G'GyrC_C Cuts at: 0 16 3066 Size: 16 3050 BanII G_rGCy'C Cuts at: 0 573 1404 3066 Size: 573 831 1662 Fragments arranged by size: 1662 831 573 BbeI G_GCGC'C Cuts at: 0 20 3066 Size: 20 3046 BbsI GAAGACnn'nnnn_ Cuts at: 0 279 1028 1806 3066 Size: 279 749 778 1260 Fragments arranged by size: 1260 778 749 279 BciVI GTATCCnnnnn_n' Cuts at: 0 799 1086 2222 2752 3066 Size: 799 287 1136 530 314 Fragments arranged by size: 1136 799 530 314 287 BfrBI ATG'CAT Cuts at: 0 1473 1965 3066 Size: 1473 492 1101 Fragments arranged by size: 1473 1101 492 BglI GCCn_nnn'nGGC Cuts at: 0 568 3066 Size: 568 2498 BlpI GC'TnA_GC Cuts at: 0 68 3066 Size: 68 2998 Bme1580I G_kGCm'C Cuts at: 0 696 911 3066 Size: 696 215 2155 Fragments arranged by size: 2155 696 215 BmgBI CAC'GTC Cuts at: 0 2857 3066 Size: 2857 209 BmrI ACTGGGnnnn_n' Cuts at: 0 655 1838 2499 3066 Size: 655 1183 661 567 Fragments arranged by size: 1183 661 655 567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 117 516 2482 3066 Size: 117 399 1966 584 Fragments arranged by size: 1966 584 399 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 553 1301 2159 3066 Size: 9 544 748 858 907 Fragments arranged by size: 907 858 748 544 9 Bpu10I CC'TnA_GC Cuts at: 0 551 3066 Size: 551 2515 BsaAI yAC'GTr Cuts at: 0 1108 1376 3066 Size: 1108 268 1690 Fragments arranged by size: 1690 1108 268 BsaHI Gr'CG_yC Cuts at: 0 17 3066 Size: 17 3049 BsaWI w'CCGG_w Cuts at: 0 972 3066 Size: 972 2094 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 1095 1125 3066 Size: 400 30 665 30 1941 Fragments arranged by size: 1941 665 400 30 30 BseYI C'CCAG_C Cuts at: 0 162 318 477 611 801 3066 Size: 162 156 159 134 190 2265 Fragments arranged by size: 2265 190 162 159 156 134 BsiHKAI G_wGCw'C Cuts at: 0 257 696 911 1113 1404 3066 Size: 257 439 215 202 291 1662 Fragments arranged by size: 1662 439 291 257 215 202 BsmI GAATG_Cn' Cuts at: 0 2291 3066 Size: 2291 775 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2601 3066 Size: 2601 465 Bsp1286I G_dGCh'C Cuts at: 0 257 573 696 911 1113 1404 3066 Size: 257 316 123 215 202 291 1662 Fragments arranged by size: 1662 316 291 257 215 202 123 BspEI T'CCGG_A Cuts at: 0 972 3066 Size: 972 2094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1839 3066 Size: 1839 1227 BssHII G'CGCG_C Cuts at: 0 176 3066 Size: 176 2890 BssSI C'ACGA_G Cuts at: 0 135 235 3066 Size: 135 100 2831 Fragments arranged by size: 2831 135 100 BstAPI GCAn_nnn'nTGC Cuts at: 0 742 3066 Size: 742 2324 BstXI CCAn_nnnn'nTGG Cuts at: 0 1302 2538 3066 Size: 1302 1236 528 Fragments arranged by size: 1302 1236 528 BstYI r'GATC_y Cuts at: 0 539 2525 3066 Size: 539 1986 541 Fragments arranged by size: 1986 541 539 BtgI C'CryG_G Cuts at: 0 468 1154 3066 Size: 468 686 1912 Fragments arranged by size: 1912 686 468 BtsI GCAGTG_nn' Cuts at: 0 740 1181 3066 Size: 740 441 1885 Fragments arranged by size: 1885 740 441 DraI TTT'AAA Cuts at: 0 1265 2315 2390 3066 Size: 1265 1050 75 676 Fragments arranged by size: 1265 1050 676 75 EaeI y'GGCC_r Cuts at: 0 83 3066 Size: 83 2983 EarI CTCTTCn'nnn_ Cuts at: 0 820 1409 2171 2410 3066 Size: 820 589 762 239 656 Fragments arranged by size: 820 762 656 589 239 EcoICRI GAG'CTC Cuts at: 0 1402 3066 Size: 1402 1664 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 329 553 718 1040 1301 2096 2159 Size: 9 320 224 165 322 261 795 63 Cuts at: 2159 2699 3066 Size: 540 367 Fragments arranged by size: 795 540 367 322 320 261 224 165 63 9 EcoO109I rG'GnC_Cy Cuts at: 0 129 527 978 3066 Size: 129 398 451 2088 Fragments arranged by size: 2088 451 398 129 EcoRI G'AATT_C Cuts at: 0 331 2739 3066 Size: 331 2408 327 Fragments arranged by size: 2408 331 327 HaeII r_GCGC'y Cuts at: 0 20 42 99 3066 Size: 20 22 57 2967 Fragments arranged by size: 2967 57 22 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 775 807 1071 1103 2261 2293 3026 3058 Size: 775 32 264 32 1158 32 733 32 Cuts at: 3058 3066 Size: 8 Fragments arranged by size: 1158 775 733 264 32 32 32 32 8 HincII GTy'rAC Cuts at: 0 1340 1732 2508 3066 Size: 1340 392 776 558 Fragments arranged by size: 1340 776 558 392 HindIII A'AGCT_T Cuts at: 0 1430 3066 Size: 1430 1636 HpaI GTT'AAC Cuts at: 0 1340 3066 Size: 1340 1726 KasI G'GCGC_C Cuts at: 0 16 3066 Size: 16 3050 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1793 3066 Size: 1793 1273 MslI CAynn'nnrTG Cuts at: 0 745 1300 1670 1960 2536 3066 Size: 745 555 370 290 576 530 Fragments arranged by size: 745 576 555 530 370 290 MspA1I CmG'CkG Cuts at: 0 89 162 318 477 636 725 801 1599 Size: 89 73 156 159 159 89 76 798 Cuts at: 1599 1635 3066 Size: 36 1431 Fragments arranged by size: 1431 798 159 159 156 89 89 76 73 36 NarI GG'CG_CC Cuts at: 0 17 3066 Size: 17 3049 NcoI C'CATG_G Cuts at: 0 468 1154 3066 Size: 468 686 1912 Fragments arranged by size: 1912 686 468 NdeI CA'TA_TG Cuts at: 0 903 1065 3066 Size: 903 162 2001 Fragments arranged by size: 2001 903 162 NsiI A_TGCA'T Cuts at: 0 1475 1967 3066 Size: 1475 492 1099 Fragments arranged by size: 1475 1099 492 NspI r_CATG'y Cuts at: 0 1959 2235 2872 3066 Size: 1959 276 637 194 Fragments arranged by size: 1959 637 276 194 PflMI CCAn_nnn'nTGG Cuts at: 0 420 1155 2197 3066 Size: 420 735 1042 869 Fragments arranged by size: 1042 869 735 420 PmlI CAC'GTG Cuts at: 0 1108 3066 Size: 1108 1958 PpuMI rG'GwC_Cy Cuts at: 0 129 527 3066 Size: 129 398 2539 Fragments arranged by size: 2539 398 129 PstI C_TGCA'G Cuts at: 0 38 80 1836 3066 Size: 38 42 1756 1230 Fragments arranged by size: 1756 1230 42 38 PvuII CAG'CTG Cuts at: 0 162 318 477 636 725 801 1635 3066 Size: 162 156 159 159 89 76 834 1431 Fragments arranged by size: 1431 834 162 159 159 156 89 76 SacI G_AGCT'C Cuts at: 0 1404 3066 Size: 1404 1662 SalI G'TCGA_C Cuts at: 0 1730 3066 Size: 1730 1336 SapI GCTCTTCn'nnn_ Cuts at: 0 1409 3066 Size: 1409 1657 ScaI AGT'ACT Cuts at: 0 1191 3066 Size: 1191 1875 SexAI A'CCwGG_T Cuts at: 0 1496 3066 Size: 1496 1570 SfcI C'TryA_G Cuts at: 0 34 76 1832 3066 Size: 34 42 1756 1234 Fragments arranged by size: 1756 1234 42 34 SfoI GGC'GCC Cuts at: 0 18 3066 Size: 18 3048 SmaI CCC'GGG Cuts at: 0 63 1116 3066 Size: 63 1053 1950 Fragments arranged by size: 1950 1053 63 SmlI C'TyrA_G Cuts at: 0 1381 3066 Size: 1381 1685 SpeI A'CTAG_T Cuts at: 0 1096 3066 Size: 1096 1970 SphI G_CATG'C Cuts at: 0 2872 3066 Size: 2872 194 SspI AAT'ATT Cuts at: 0 2961 3066 Size: 2961 105 StyI C'CwwG_G Cuts at: 0 468 1154 2816 3066 Size: 468 686 1662 250 Fragments arranged by size: 1662 686 468 250 SwaI ATTT'AAAT Cuts at: 0 2315 3066 Size: 2315 751 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2022 2951 3063 3066 Size: 2022 929 112 3 Fragments arranged by size: 2022 929 112 3 TatI w'GTAC_w Cuts at: 0 2 1189 3066 Size: 2 1187 1877 Fragments arranged by size: 1877 1187 2 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 400 432 1095 1127 3066 Size: 400 32 663 32 1939 Fragments arranged by size: 1939 663 400 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2033 3066 Size: 2033 1033 XbaI T'CTAG_A Cuts at: 0 2433 3066 Size: 2433 633 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 921 3066 Size: 921 2145 XhoI C'TCGA_G Cuts at: 0 1381 3066 Size: 1381 1685 XmaI C'CCGG_G Cuts at: 0 61 1114 3066 Size: 61 1053 1952 Fragments arranged by size: 1952 1053 61 XmnI GAAnn'nnTTC Cuts at: 0 203 493 769 1973 2800 2833 3008 3066 Size: 203 290 276 1204 827 33 175 58 Fragments arranged by size: 1204 827 290 276 203 175 58 33 Enzymes that do cut: AccI AcuI AflIII AleI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI MspA1I NarI NcoI NdeI NsiI NspI PflMI PmlI PpuMI PstI PvuII SacI SalI SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI AscI AsiSI AvrII BaeI BbvCI BcgI BclI BglII BmtI BpuEI BsaI BsaBI BseRI BsgI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI KpnI MfeI MluI MscI NaeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI SfiI SgfI SgrAI SnaBI SrfI StuI ZraI