################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccIII AflII AflIII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspI AvrII BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoO65I EcoRV FbaI FseI HincII HindII HpaI KspI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1495 GGTACC G^GTACC AccI 36 GTCTAC GT^[AC][GT]AC AclI 1787 AACGTT AA^CGTT AhdI 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2354 AGCGCT AGC^GCT Asp700I 1815 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1495 GGTACC G^GTACC AspEI 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2321 TGCGCA TGC^GCA BamHI 1922 GGATCC G^GATCC BfaI 424 CTAG C^TAG BsaBI 243 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 169 GAGACG CGTCTC(1/5) BsrFI 1349 GCCGGC [GA]^CCGG[CT] Cfr10I 1349 GCCGGC [GA]^CCGG[CT] Eam1105I 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2354 AGCGCT AGC^GCT EcoNI 1929 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1262 GAATTC G^AATTC EcoT22I 1780 ATGCAT ATGCA^T FspI 2321 TGCGCA TGC^GCA HindIII 2746 AAGCTT A^AGCTT KpnI 1495 GGTACC GGTAC^C MaeI 424 CTAG C^TAG MamI 243 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 2451 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1349 GCCGGC GCC^GGC NgoMIV 1349 GCCGGC G^CCGGC NsiI 1780 ATGCAT ATGCA^T NspI 2603 ACATGC [GA]CATG^[CT] PflMI 2664 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1241 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1787 AACGTT AA^CGTT ScaI 2294 AGTACT AGT^ACT SexAI 1951 ACCAGGT A^CC[AT]GGT StuI 381 AGGCCT AGG^CCT Van91I 2664 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1397 CCAATGGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1815 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 1262 GAATTC [GA]^AATT[CT] 1594 AAATTT ApoI 1262 GAATTC [GA]^AATT[CT] 1594 AAATTT BbeI 133 GGCGCC GGCGC^C 1685 GGCGCC BbsI 549 GAAGAC GAAGAC(2/6) 955 GAAGAC BbvCI 1308 CCTCAGC CCTCAGC(-5/-2) 922 GCTGAGG BpuAI 549 GAAGAC GAAGAC(2/6) 955 GAAGAC BsgI 442 CTGCAC GTGCAG(16/14) 1470 CTGCAC BsiEI 960 CGGCCG CG[GA][CT]^CG 2752 CGGCCG Bsp1407I 1565 TGTACA T^GTACA 2459 TGTACA BspHI 323 TCATGA T^CATGA 2186 TCATGA BspMI 259 ACCTGC ACCTGC(4/8) 1416 ACCTGC BsrBI 2338 GAGCGG CCGCTC(-3/-3) 2560 GAGCGG BsrGI 1565 TGTACA T^GTACA 2459 TGTACA DsaI 350 CCGTGG C^C[GA][CT]GG 989 CCGTGG EagI 960 CGGCCG C^GGCCG 2752 CGGCCG EclXI 960 CGGCCG C^GGCCG 2752 CGGCCG Eco52I 960 CGGCCG C^GGCCG 2752 CGGCCG EcoT14I 929 CCAAGG C^C[AT][AT]GG 1376 CCTTGG KasI 133 GGCGCC G^GCGCC 1685 GGCGCC NarI 133 GGCGCC GG^CGCC 1685 GGCGCC PaeR7I 499 CTCGAG C^TCGAG 1072 CTCGAG RcaI 323 TCATGA T^CATGA 2186 TCATGA SacI 715 GAGCTC GAGCT^C 2200 GAGCTC SfoI 133 GGCGCC GGC^GCC 1685 GGCGCC SspBI 1565 TGTACA T^GTACA 2459 TGTACA StyI 929 CCAAGG C^C[AT][AT]GG 1376 CCTTGG TfiI 543 GAATC G^A[AT]TC 568 GATTC XhoI 499 CTCGAG C^TCGAG 1072 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII