(Linear) (Six-base) MAPSORT of: KIEE3334.seq Check: 3809 from: 1 to: 2904 KIEE3334.orf With 182 enzymes: SgfI * July 3, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 268 1425 2904 Size: 268 1157 1479 Fragments arranged by size: 1479 1157 268 AccI GT'mk_AC Cuts at: 0 37 2904 Size: 37 2867 Acc65I G'GTAC_C Cuts at: 0 1495 2904 Size: 1495 1409 AclI AA'CG_TT Cuts at: 0 1788 2904 Size: 1788 1116 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 832 2404 2716 2749 2904 Size: 568 264 1572 312 33 155 Fragments arranged by size: 1572 568 312 264 155 33 AfeI AGC'GCT Cuts at: 0 2356 2904 Size: 2356 548 AhdI GACnn_n'nnGTC Cuts at: 0 660 2904 Size: 660 2244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1907 2904 Size: 1907 997 AlwNI CAG_nnn'CTG Cuts at: 0 864 1219 1469 2646 2694 2727 2904 Size: 864 355 250 1177 48 33 177 Fragments arranged by size: 1177 864 355 250 177 48 33 ApoI r'AATT_y Cuts at: 0 1262 1594 2904 Size: 1262 332 1310 Fragments arranged by size: 1310 1262 332 AvaI C'yCGr_G Cuts at: 0 499 601 1072 2904 Size: 499 102 471 1832 Fragments arranged by size: 1832 499 471 102 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1479 1512 2904 Size: 1479 33 1392 Fragments arranged by size: 1479 1392 33 BamHI G'GATC_C Cuts at: 0 1922 2904 Size: 1922 982 BanI G'GyrC_C Cuts at: 0 133 1098 1495 1685 2261 2904 Size: 133 965 397 190 576 643 Fragments arranged by size: 965 643 576 397 190 133 BanII G_rGCy'C Cuts at: 0 395 719 1133 1540 2204 2904 Size: 395 324 414 407 664 700 Fragments arranged by size: 700 664 414 407 395 324 BbeI G_GCGC'C Cuts at: 0 137 1689 2904 Size: 137 1552 1215 Fragments arranged by size: 1552 1215 137 BbsI GAAGACnn'nnnn_ Cuts at: 0 556 962 2904 Size: 556 406 1942 Fragments arranged by size: 1942 556 406 BbvCI CC'TCA_GC Cuts at: 0 923 1309 2904 Size: 923 386 1595 Fragments arranged by size: 1595 923 386 BfrBI ATG'CAT Cuts at: 0 1782 2904 Size: 1782 1122 BglII A'GATC_T Cuts at: 0 468 1327 2159 2904 Size: 468 859 832 745 Fragments arranged by size: 859 832 745 468 BlpI GC'TnA_GC Cuts at: 0 1292 2501 2821 2904 Size: 1292 1209 320 83 Fragments arranged by size: 1292 1209 320 83 Bme1580I G_kGCm'C Cuts at: 0 1226 2842 2904 Size: 1226 1616 62 Fragments arranged by size: 1616 1226 62 BmgBI CAC'GTC Cuts at: 0 75 2904 Size: 75 2829 BmrI ACTGGGnnnn_n' Cuts at: 0 842 1174 2904 Size: 842 332 1730 Fragments arranged by size: 1730 842 332 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1554 2904 Size: 1554 1350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 388 886 1030 1390 1415 1429 1699 Size: 244 144 498 144 360 25 14 270 Cuts at: 1699 1792 1867 1951 2137 2287 2904 Size: 93 75 84 186 150 617 Fragments arranged by size: 617 498 360 270 244 186 150 144 144 93 84 75 25 14 Bpu10I CC'TnA_GC Cuts at: 0 923 1309 2904 Size: 923 386 1595 Fragments arranged by size: 1595 923 386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1564 2904 Size: 1564 1340 BsaI GGTCTCn'nnnn_ Cuts at: 0 613 1366 2204 2904 Size: 613 753 838 700 Fragments arranged by size: 838 753 700 613 BsaBI GATnn'nnATC Cuts at: 0 247 2904 Size: 247 2657 BsaHI Gr'CG_yC Cuts at: 0 134 356 986 1686 2904 Size: 134 222 630 700 1218 Fragments arranged by size: 1218 700 630 222 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2164 2194 2821 2851 2904 Size: 2164 30 627 30 53 Fragments arranged by size: 2164 627 53 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 229 232 994 1020 1090 1681 1924 2068 Size: 229 3 762 26 70 591 243 144 Cuts at: 2068 2445 2554 2842 2904 Size: 377 109 288 62 Fragments arranged by size: 762 591 377 288 243 229 144 109 70 62 26 3 BseYI C'CCAG_C Cuts at: 0 123 1102 1314 1742 2531 2904 Size: 123 979 212 428 789 373 Fragments arranged by size: 979 789 428 373 212 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 1455 2904 Size: 427 1028 1449 Fragments arranged by size: 1449 1028 427 BsiEI CG_ry'CG Cuts at: 0 963 2755 2904 Size: 963 1792 149 Fragments arranged by size: 1792 963 149 BsiHKAI G_wGCw'C Cuts at: 0 74 719 2060 2204 2246 2681 2904 Size: 74 645 1341 144 42 435 223 Fragments arranged by size: 1341 645 435 223 144 74 42 BsmBI CGTCTCn'nnnn_ Cuts at: 0 163 2904 Size: 163 2741 Bsp1286I G_dGCh'C Cuts at: 0 74 395 719 1133 1226 1540 2060 2204 Size: 74 321 324 414 93 314 520 144 Cuts at: 2204 2246 2681 2842 2904 Size: 42 435 161 62 Fragments arranged by size: 520 435 414 324 321 314 161 144 93 74 62 42 BspHI T'CATG_A Cuts at: 0 323 2186 2904 Size: 323 1863 718 Fragments arranged by size: 1863 718 323 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 268 1425 2904 Size: 268 1157 1479 Fragments arranged by size: 1479 1157 268 BsrBI CCG'CTC Cuts at: 0 2340 2562 2904 Size: 2340 222 342 Fragments arranged by size: 2340 342 222 BsrFI r'CCGG_y Cuts at: 0 1349 2904 Size: 1349 1555 BsrGI T'GTAC_A Cuts at: 0 1565 2459 2904 Size: 1565 894 445 Fragments arranged by size: 1565 894 445 BssSI C'ACGA_G Cuts at: 0 712 2311 2584 2904 Size: 712 1599 273 320 Fragments arranged by size: 1599 712 320 273 BstAPI GCAn_nnn'nTGC Cuts at: 0 1469 1764 2250 2316 2646 2904 Size: 1469 295 486 66 330 258 Fragments arranged by size: 1469 486 330 295 258 66 BstXI CCAn_nnnn'nTGG Cuts at: 0 366 1095 1147 2904 Size: 366 729 52 1757 Fragments arranged by size: 1757 729 366 52 BstYI r'GATC_y Cuts at: 0 248 468 1156 1327 1922 2159 2423 2778 Size: 248 220 688 171 595 237 264 355 Cuts at: 2778 2904 Size: 126 Fragments arranged by size: 688 595 355 264 248 237 220 171 126 BtgI C'CryG_G Cuts at: 0 350 989 2904 Size: 350 639 1915 Fragments arranged by size: 1915 639 350 BtsI GCAGTG_nn' Cuts at: 0 1220 2620 2904 Size: 1220 1400 284 Fragments arranged by size: 1400 1220 284 EaeI y'GGCC_r Cuts at: 0 188 254 960 1394 2123 2453 2752 2904 Size: 188 66 706 434 729 330 299 152 Fragments arranged by size: 729 706 434 330 299 188 152 66 EagI C'GGCC_G Cuts at: 0 960 2752 2904 Size: 960 1792 152 Fragments arranged by size: 1792 960 152 EarI CTCTTCn'nnn_ Cuts at: 0 43 941 971 1718 2125 2234 2461 2539 Size: 43 898 30 747 407 109 227 78 Cuts at: 2539 2768 2904 Size: 229 136 Fragments arranged by size: 898 747 407 229 227 136 109 78 43 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 7 67 1034 1915 1935 2848 2904 Size: 7 60 967 881 20 913 56 Fragments arranged by size: 967 913 881 60 56 20 7 EcoICRI GAG'CTC Cuts at: 0 717 2202 2904 Size: 717 1485 702 Fragments arranged by size: 1485 717 702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 388 568 832 886 1030 1390 1415 Size: 244 144 180 264 54 144 360 25 Cuts at: 1415 1429 1699 1792 1867 1951 2137 2287 2404 Size: 14 270 93 75 84 186 150 117 Cuts at: 2404 2716 2749 2904 Size: 312 33 155 Fragments arranged by size: 360 312 270 264 244 186 180 155 150 144 144 117 93 84 75 54 33 25 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 1933 2904 Size: 1933 971 EcoO109I rG'GnC_Cy Cuts at: 0 732 1305 1435 1554 2112 2700 2904 Size: 732 573 130 119 558 588 204 Fragments arranged by size: 732 588 573 558 204 130 119 EcoRI G'AATT_C Cuts at: 0 1262 2904 Size: 1262 1642 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1839 2904 Size: 1839 1065 FspI TGC'GCA Cuts at: 0 2323 2904 Size: 2323 581 HaeII r_GCGC'y Cuts at: 0 137 1367 1689 2358 2904 Size: 137 1230 322 669 546 Fragments arranged by size: 1230 669 546 322 137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 646 678 985 1017 1524 1556 2904 Size: 646 32 307 32 507 32 1348 Fragments arranged by size: 1348 646 507 307 32 32 32 HindIII A'AGCT_T Cuts at: 0 2746 2904 Size: 2746 158 KasI G'GCGC_C Cuts at: 0 133 1685 2904 Size: 133 1552 1219 Fragments arranged by size: 1552 1219 133 KpnI G_GTAC'C Cuts at: 0 1499 2904 Size: 1499 1405 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 734 2537 2904 Size: 734 1803 367 Fragments arranged by size: 1803 734 367 MscI TGG'CCA Cuts at: 0 256 1396 2125 2455 2904 Size: 256 1140 729 330 449 Fragments arranged by size: 1140 729 449 330 256 MslI CAynn'nnrTG Cuts at: 0 2455 2904 Size: 2455 449 MspA1I CmG'CkG Cuts at: 0 861 1095 1746 1770 2025 2250 2268 2307 Size: 861 234 651 24 255 225 18 39 Cuts at: 2307 2382 2500 2646 2904 Size: 75 118 146 258 Fragments arranged by size: 861 651 258 255 234 225 146 118 75 39 24 18 NaeI GCC'GGC Cuts at: 0 1351 2904 Size: 1351 1553 NarI GG'CG_CC Cuts at: 0 134 1686 2904 Size: 134 1552 1218 Fragments arranged by size: 1552 1218 134 NgoMIV G'CCGG_C Cuts at: 0 1349 2904 Size: 1349 1555 NsiI A_TGCA'T Cuts at: 0 1784 2904 Size: 1784 1120 NspI r_CATG'y Cuts at: 0 2607 2904 Size: 2607 297 PasI CC'CwG_GG Cuts at: 0 666 2904 Size: 666 2238 PflMI CCAn_nnn'nTGG Cuts at: 0 2670 2904 Size: 2670 234 PpuMI rG'GwC_Cy Cuts at: 0 732 1305 1554 2112 2904 Size: 732 573 249 558 792 Fragments arranged by size: 792 732 573 558 249 PshAI GACnn'nnGTC Cuts at: 0 1245 2904 Size: 1245 1659 PspXI vC'TCGA_Gb Cuts at: 0 499 2904 Size: 499 2405 PstI C_TGCA'G Cuts at: 0 1138 1462 1769 2255 2570 2642 2651 2904 Size: 1138 324 307 486 315 72 9 253 Fragments arranged by size: 1138 486 324 315 307 253 72 9 PvuII CAG'CTG Cuts at: 0 861 1746 1770 2250 2268 2382 2500 2646 Size: 861 885 24 480 18 114 118 146 Cuts at: 2646 2904 Size: 258 Fragments arranged by size: 885 861 480 258 146 118 114 24 18 SacI G_AGCT'C Cuts at: 0 719 2204 2904 Size: 719 1485 700 Fragments arranged by size: 1485 719 700 SapI GCTCTTCn'nnn_ Cuts at: 0 1718 2234 2461 2904 Size: 1718 516 227 443 Fragments arranged by size: 1718 516 443 227 ScaI AGT'ACT Cuts at: 0 2296 2904 Size: 2296 608 SexAI A'CCwGG_T Cuts at: 0 1951 2904 Size: 1951 953 SfcI C'TryA_G Cuts at: 0 556 791 1134 1458 1765 2251 2566 2638 Size: 556 235 343 324 307 486 315 72 Cuts at: 2638 2647 2883 2904 Size: 9 236 21 Fragments arranged by size: 556 486 343 324 315 307 236 235 72 21 9 SfoI GGC'GCC Cuts at: 0 135 1687 2904 Size: 135 1552 1217 Fragments arranged by size: 1552 1217 135 SmlI C'TyrA_G Cuts at: 0 499 1072 1579 2904 Size: 499 573 507 1325 Fragments arranged by size: 1325 573 507 499 StuI AGG'CCT Cuts at: 0 383 2904 Size: 383 2521 StyI C'CwwG_G Cuts at: 0 929 1376 2904 Size: 929 447 1528 Fragments arranged by size: 1528 929 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1116 1369 2904 Size: 1116 253 1535 Fragments arranged by size: 1535 1116 253 TatI w'GTAC_w Cuts at: 0 1565 2294 2459 2904 Size: 1565 729 165 445 Fragments arranged by size: 1565 729 445 165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2164 2196 2821 2853 2904 Size: 2164 32 625 32 51 Fragments arranged by size: 2164 625 51 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1404 2904 Size: 1404 1500 XhoI C'TCGA_G Cuts at: 0 499 1072 2904 Size: 499 573 1832 Fragments arranged by size: 1832 573 499 XmnI GAAnn'nnTTC Cuts at: 0 1819 2904 Size: 1819 1085 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AhdI AlfI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PflMI PpuMI PshAI PspXI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AflII AflIII AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BcgI BciVI BclI BglI BmtI BsaAI BsaWI BsiWI BsmI BspEI BsrDI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRV FseI FspAI HincII HpaI MfeI MluI NcoI NdeI NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI ZraI