Restriction map for
pFN21AE3454
(Circular) (Six-base) MAPSORT of: pFN21AE3454.seq Check: 7673 from: 1 to: 8689
pFN21AE3454 8689 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6499 278
Size: 53 83 186 936 4963 2468
Fragments arranged by size:
4963 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2212 4457 5229 6032 1937
Size: 275 2245 772 803 4594
Fragments arranged by size:
4594 2245 803 772 275
Acc65I G'GTAC_C
Cuts at: 6010 6010
Size: 8689
AclI AA'CG_TT
Cuts at: 1563 6816 7189 1563
Size: 5253 373 3063
Fragments arranged by size:
5253 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2595 3102 3693 4302 4542 5190 5981
Size: 941 22 507 591 609 240 648 791
Cuts at: 5981 6752 8136 8404 1632
Size: 771 1384 268 1917
Fragments arranged by size:
1917 1384 941 791 771 648 609 591
507 268 240 22
AflII C'TTAA_G
Cuts at: 820 1017 3935 4840 5675 820
Size: 197 2918 905 835 3834
Fragments arranged by size:
3834 2918 905 835 197
AflIII A'CryG_T
Cuts at: 1203 2868 4050 5362 7588 8565 1203
Size: 1665 1182 1312 2226 977 1327
Fragments arranged by size:
2226 1665 1327 1312 1182 977
AgeI A'CCGG_T
Cuts at: 7520 7520
Size: 8689
AhdI GACnn_n'nnGTC
Cuts at: 7418 7418
Size: 8689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8689
AlwNI CAG_nnn'CTG
Cuts at: 2756 4994 5959 6432 8004 2756
Size: 2238 965 473 1572 3441
Fragments arranged by size:
3441 2238 1572 965 473
ApaLI G'TGCA_C
Cuts at: 6746 7902 6746
Size: 1156 7533
ApoI r'AATT_y
Cuts at: 1493 3007 3598 3655 3720 4350 4403 4591 4659
Size: 1514 591 57 65 630 53 188 68
Cuts at: 4659 4717 4852 5117 5998 6224 6376 8485 1493
Size: 58 135 265 881 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 881 630 591 265 226
188 152 135 68 65 58 57 53
AseI AT'TA_AT
Cuts at: 160 1032 7243 160
Size: 872 6211 1606
Fragments arranged by size:
6211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
AvaI C'yCGr_G
Cuts at: 1870 1943 6014 1870
Size: 73 4071 4545
Fragments arranged by size:
4545 4071 73
AvrII C'CTAG_G
Cuts at: 3767 3767
Size: 8689
BamHI G'GATC_C
Cuts at: 2824 6019 6386 2824
Size: 3195 367 5127
Fragments arranged by size:
5127 3195 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 5657 6010 7465 618
Size: 325 217 627 1661 2209 353 1455 1842
Fragments arranged by size:
2209 1842 1661 1455 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2397 2460 2778 2806 5094 6008 1391
Size: 1006 63 318 28 2288 914 4072
Fragments arranged by size:
4072 2288 1006 914 318 63 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 2832 3650 4308 4569 928
Size: 971 408 525 818 658 261 5048
Fragments arranged by size:
5048 971 818 658 525 408 261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6880 6914 1903
Size: 34 4943 34 3678
Fragments arranged by size:
4943 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5278 6581 7791 5278
Size: 1303 1210 6176
Fragments arranged by size:
6176 1303 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8689
BfrBI ATG'CAT
Cuts at: 3697 5686 3697
Size: 1989 6700
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7300 136
Size: 107 122 71 631 605 5628 1525
Fragments arranged by size:
5628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2492 3931 4510 8684 2492
Size: 1439 579 4174 2497
Fragments arranged by size:
4174 2497 1439 579
BlpI GC'TnA_GC
Cuts at: 2777 6106 2777
Size: 3329 5360
Bme1580I G_kGCm'C
Cuts at: 1790 2350 4581 6750 7906 1790
Size: 560 2231 2169 1156 2573
Fragments arranged by size:
2573 2231 2169 1156 560
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 4575 6420
Size: 416 53 83 399 24 779 2379 1845
Cuts at: 6420 7378 442
Size: 958 1753
Fragments arranged by size:
2379 1845 1753 958 779 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2930 5776 5936 7349 1239
Size: 1691 2846 160 1413 2579
Fragments arranged by size:
2846 2579 1691 1413 160
Bpu10I CC'TnA_GC
Cuts at: 2396 2742 4380 5093 2396
Size: 346 1638 713 5992
Fragments arranged by size:
5992 1638 713 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6814 7679 7977 6814
Size: 865 298 7526
Fragments arranged by size:
7526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 7352 882
Size: 617 3744 2109 2219
Fragments arranged by size:
3744 2219 2109 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 6480 493
Size: 711 2849 2427 2702
Fragments arranged by size:
2849 2702 2427 711
BsaBI GATnn'nnATC
Cuts at: 8683 8683
Size: 8689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6496 6878 7513 275
Size: 53 83 186 936 4963 382 635 1451
Fragments arranged by size:
4963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 5319 7122 7520 7794 7941 8352 3816
Size: 1503 1803 398 274 147 411 4153
Fragments arranged by size:
4153 1803 1503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2793 2823 3501 3531 5014
Size: 30 165 30 1390 30 678 30 1483
Cuts at: 5014 5044 5749 5779 1178
Size: 30 705 30 4088
Fragments arranged by size:
4088 1483 1390 705 678 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2532 5126 1186
Size: 970 376 2594 4749
Fragments arranged by size:
4749 2594 970 376
BseYI C'CCAG_C
Cuts at: 3195 4569 7892 8388 3195
Size: 1374 3323 496 3496
Fragments arranged by size:
3496 3323 1374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6139 6900 7928 1540
Size: 275 186 4138 761 1028 2301
Fragments arranged by size:
4138 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2460 2778 2876 4212 5848 6750 6835 7906
Size: 63 318 98 1336 1636 902 85 1071
Cuts at: 7906 2397
Size: 3180
Fragments arranged by size:
3180 1636 1336 1071 902 318 98 85
63
BsmI GAATG_Cn'
Cuts at: 3199 6200 6293 3199
Size: 3001 93 5595
Fragments arranged by size:
5595 3001 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2350 2397 2460 2778 2806 2876 4212
Size: 399 560 47 63 318 28 70 1336
Cuts at: 4212 4581 5094 5848 6008 6750 6835 7906 1391
Size: 369 513 754 160 742 85 1071 2174
Fragments arranged by size:
2174 1336 1071 754 742 560 513 399
369 318 160 85 70 63 47 28
BspEI T'CCGG_A
Cuts at: 8352 8352
Size: 8689
BspHI T'CATG_A
Cuts at: 2059 4789 6578 8308 2059
Size: 2730 1789 1730 2440
Fragments arranged by size:
2730 2440 1789 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5636 6044 844
Size: 4792 408 3489
Fragments arranged by size:
4792 3489 408
BsrBI CCG'CTC
Cuts at: 1394 1823 6576 1394
Size: 429 4753 3507
Fragments arranged by size:
4753 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2042 2470 3291 5474 7184 7358 65
Size: 1183 794 428 821 2183 1710 174 1396
Fragments arranged by size:
2183 1710 1396 1183 821 794 428 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7333 7520 1700
Size: 110 5523 187 2869
Fragments arranged by size:
5523 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3568 4249 4606 96
Size: 3472 681 357 4179
Fragments arranged by size:
4179 3472 681 357
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8689
BssSI C'ACGA_G
Cuts at: 6749 7761 6749
Size: 1012 7677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8689
BstBI TT'CG_AA
Cuts at: 7493 7493
Size: 8689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8689
BstXI CCAn_nnnn'nTGG
Cuts at: 4366 5635 4366
Size: 1269 7420
BstYI r'GATC_y
Cuts at: 1972 2197 2492 2824 3151 3931 4510 4542 4990
Size: 225 295 332 327 780 579 32 448
Cuts at: 4990 6019 6386 6771 6788 8240 8326 8338 8684
Size: 1029 367 385 17 1452 86 12 346
Cuts at: 8684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1029 780 579 448 385 367
346 332 327 295 225 86 32 17
12
BstZ17I GTA'TAC
Cuts at: 5230 5230
Size: 8689
BtgI C'CryG_G
Cuts at: 513 1065 3058 5811 513
Size: 552 1993 2753 3391
Fragments arranged by size:
3391 2753 1993 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2316 5540 487
Size: 683 56 1090 3224 3636
Fragments arranged by size:
3636 3224 1090 683 56
BtsI GCAGTG_nn'
Cuts at: 4581 6205 6999 7019 4581
Size: 1624 794 20 6251
Fragments arranged by size:
6251 1624 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8654
DraI TTT'AAA
Cuts at: 5163 5652 5993 5163
Size: 489 341 7859
Fragments arranged by size:
7859 489 341
DraIII CAC_nnn'GTG
Cuts at: 4121 4121
Size: 8689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2836 7696 809
Size: 507 1520 4860 1802
Fragments arranged by size:
4860 1802 1520 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2608 5823 6136
Size: 54 1425 50 275 18 778 3215 313
Cuts at: 6136 7025 8654 8
Size: 889 1629 43
Fragments arranged by size:
3215 1629 1425 889 778 313 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6136 1537
Size: 275 4324 4090
Fragments arranged by size:
4324 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 2571 3036 3081 3222 3303 4124 4446
Size: 724 494 465 45 141 81 821 322
Cuts at: 4446 4521 6619 1353
Size: 75 2098 3423
Fragments arranged by size:
3423 2098 821 724 494 465 322 141
81 75 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7276 7650 7796 387
Size: 1064 138 5687 374 146 1280
Fragments arranged by size:
5687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2458 2776 2395
Size: 63 318 8308
Fragments arranged by size:
8308 318 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 2595 2930 3102 3693 4302 4542
Size: 393 941 22 335 172 591 609 240
Cuts at: 4542 5190 5776 5936 5981 6752 7349 8136 8404
Size: 648 586 160 45 771 597 787 268
Cuts at: 8404 1239
Size: 1524
Fragments arranged by size:
1524 941 787 771 648 609 597 591
586 393 335 268 240 172 160 45
22
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 2912
Size: 8689
EcoO109I rG'GnC_Cy
Cuts at: 3677 4005 3677
Size: 328 8361
EcoRI G'AATT_C
Cuts at: 3598 4403 4591 5998 6376 3598
Size: 805 188 1407 378 5911
Fragments arranged by size:
5911 1407 805 378 188
EcoRV GAT'ATC
Cuts at: 5563 5752 5563
Size: 189 8500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4767 5986 4767
Size: 1219 7470
FspI TGC'GCA
Cuts at: 5909 7195 5909
Size: 1286 7403
FspAI rTGC'GCAy
Cuts at: 5909 5909
Size: 8689
HaeII r_GCGC'y
Cuts at: 1678 7836 1678
Size: 6158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2649 2681 2878
Size: 32 135 32 482 32 563 32 197
Cuts at: 2878 2910 3436 3468 3972 4004 4319 4351 4946
Size: 32 526 32 504 32 315 32 595
Cuts at: 4946 4978 5867 5899 7330 7362 7404 7436 1373
Size: 32 889 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 889 595 563 526 504 482
315 197 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3559 5368 6033 6279 669
Size: 988 281 1621 1809 665 246 3079
Fragments arranged by size:
3079 1809 1621 988 665 281 246
HindIII A'AGCT_T
Cuts at: 748 3328 748
Size: 2580 6109
HpaI GTT'AAC
Cuts at: 6279 6279
Size: 8689
KpnI G_GTAC'C
Cuts at: 6014 6014
Size: 8689
MfeI C'AATT_G
Cuts at: 3761 6288 3761
Size: 2527 6162
MluI A'CGCG_T
Cuts at: 8565 8565
Size: 8689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 2852 3597 7803 7987 1305
Size: 797 750 745 4206 184 2007
Fragments arranged by size:
4206 2007 797 750 745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6867
Fragments arranged by size:
6867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 3276 5085 5420 6647 7006 7165
Size: 648 1207 903 1809 335 1227 359 159
Cuts at: 7165 518
Size: 2042
Fragments arranged by size:
2042 1809 1227 1207 903 648 359 335
159
MspA1I CmG'CkG
Cuts at: 1606 4569 6105 6782 7930 8175 1606
Size: 2963 1536 677 1148 245 2120
Fragments arranged by size:
2963 2120 1536 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3058 5811 513
Size: 552 1993 2753 3391
Fragments arranged by size:
3391 2753 1993 552
NdeI CA'TA_TG
Cuts at: 387 2595 4170 387
Size: 2208 1575 4906
Fragments arranged by size:
4906 2208 1575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8689
NotI GC'GGCC_GC
Cuts at: 6136 6136
Size: 8689
NruI TCG'CGA
Cuts at: 5559 5559
Size: 8689
NsiI A_TGCA'T
Cuts at: 3699 5688 3699
Size: 1989 6700
NspI r_CATG'y
Cuts at: 1132 1229 2036 2360 2872 5366 5394 5674 5914
Size: 97 807 324 512 2494 28 280 240
Cuts at: 5914 6047 7592 1132
Size: 133 1545 2229
Fragments arranged by size:
2494 2229 1545 807 512 324 280 240
133 97 28
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8689
PciI A'CATG_T
Cuts at: 2868 5362 7588 2868
Size: 2494 2226 3969
Fragments arranged by size:
3969 2494 2226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3756 4975 1108
Size: 2648 1219 4822
Fragments arranged by size:
4822 2648 1219
PfoI T'CCnGG_A
Cuts at: 2797 3865 2797
Size: 1068 7621
PmeI GTTT'AAAC
Cuts at: 5993 5993
Size: 8689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 6758 6790 8271 8303 5474
Size: 32 1252 32 1481 32 5860
Fragments arranged by size:
5860 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3677 4005 3677
Size: 328 8361
PshAI GACnn'nnGTC
Cuts at: 1613 2055 2166 1613
Size: 442 111 8136
Fragments arranged by size:
8136 442 111
PsiI TTA'TAA
Cuts at: 5459 6259 5459
Size: 800 7889
PstI C_TGCA'G
Cuts at: 830 2168 3034 4930 6041 830
Size: 1338 866 1896 1111 3478
Fragments arranged by size:
3478 1896 1338 1111 866
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8689
PvuII CAG'CTG
Cuts at: 4569 4569
Size: 8689
SacI G_AGCT'C
Cuts at: 2397 2460 2778 2397
Size: 63 318 8308
Fragments arranged by size:
8308 318 63
SalI G'TCGA_C
Cuts at: 1936 6031 1936
Size: 4095 4594
SanDI GG'GwC_CC
Cuts at: 4005 4005
Size: 8689
SapI GCTCTTCn'nnn_
Cuts at: 4124 4521 4124
Size: 397 8292
SbfI CC_TGCA'GG
Cuts at: 6041 6041
Size: 8689
ScaI AGT'ACT
Cuts at: 6937 8649 6937
Size: 1712 6977
SfcI C'TryA_G
Cuts at: 826 1045 2164 3030 4926 5327 5787 6037 7172
Size: 219 1119 866 1896 401 460 250 1135
Cuts at: 7172 7853 8044 826
Size: 681 191 1471
Fragments arranged by size:
1896 1471 1135 1119 866 681 460 401
250 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8689
SmaI CCC'GGG
Cuts at: 1872 6016 1872
Size: 4144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3935 4840 5675 6793 7694 7956
Size: 197 926 1992 905 835 1118 901 262
Cuts at: 7956 820
Size: 1553
Fragments arranged by size:
1992 1553 1118 926 905 901 835 262
197
SnaBI TAC'GTA
Cuts at: 493 6480 493
Size: 5987 2702
SpeI A'CTAG_T
Cuts at: 152 5243 152
Size: 5091 3598
SphI G_CATG'C
Cuts at: 1132 6047 1132
Size: 4915 3774
SspI AAT'ATT
Cuts at: 5 52 3025 3301 4769 6613 5
Size: 47 2973 276 1468 1844 2081
Fragments arranged by size:
2973 2081 1844 1468 276 47
StuI AGG'CCT
Cuts at: 1517 3754 1517
Size: 2237 6452
StyI C'CwwG_G
Cuts at: 513 1065 2934 3058 3489 3755 3767 4242 5190
Size: 552 1869 124 431 266 12 475 948
Cuts at: 5190 5811 6128 513
Size: 621 317 3074
Fragments arranged by size:
3074 1869 948 621 552 475 431 317
266 124 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5675 6716 6733 6886 7071 7516 325
Size: 1255 4095 1041 17 153 185 445 1498
Fragments arranged by size:
4095 1498 1255 1041 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3314 3319
Size: 274 80 33 51 1443 624 713 5
Cuts at: 3319 3568 4249 4606 5768 6935 8647 96
Size: 249 681 357 1162 1167 1712 138
Fragments arranged by size:
1712 1443 1167 1162 713 681 624 357
274 249 138 80 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 3708 3740 5179 5211 8649 8681 2224
Size: 32 1452 32 1439 32 3438 32 2232
Fragments arranged by size:
3438 2232 1452 1439 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8689
XbaI T'CTAG_A
Cuts at: 4675 6025 4675
Size: 1350 7339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5818 5818
Size: 8689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8689
XmaI C'CCGG_G
Cuts at: 1870 6014 1870
Size: 4144 4545
XmnI GAAnn'nnTTC
Cuts at: 4403 6818 4403
Size: 2415 6274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6497 276
Size: 53 83 186 936 4963 2468
Fragments arranged by size:
4963 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI BsiWI BsmBI Bsu36I FseI KasI NaeI NarI
NgoMIV PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI