(Linear) (Six-base) MAPSORT of: KIEE3454.seq Check: 7203 from: 1 to: 3984 KIEE3454.orf With 182 enzymes: SgfI * July 3, 2012 17:53 .. AccI GT'mk_AC Cuts at: 0 207 2452 3224 3984 Size: 207 2245 772 760 Fragments arranged by size: 2245 772 760 207 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 590 1097 1688 2297 2537 3185 3976 Size: 568 22 507 591 609 240 648 791 Cuts at: 3976 3984 Size: 8 Fragments arranged by size: 791 648 609 591 568 507 240 22 8 AflII C'TTAA_G Cuts at: 0 1930 2835 3670 3984 Size: 1930 905 835 314 Fragments arranged by size: 1930 905 835 314 AflIII A'CryG_T Cuts at: 0 863 2045 3357 3984 Size: 863 1182 1312 627 Fragments arranged by size: 1312 1182 863 627 AlwNI CAG_nnn'CTG Cuts at: 0 751 2989 3954 3984 Size: 751 2238 965 30 Fragments arranged by size: 2238 965 751 30 ApoI r'AATT_y Cuts at: 0 1002 1593 1650 1715 2345 2398 2586 2654 Size: 1002 591 57 65 630 53 188 68 Cuts at: 2654 2712 2847 3112 3984 Size: 58 135 265 872 Fragments arranged by size: 1002 872 630 591 265 188 135 68 65 58 57 53 AvrII C'CTAG_G Cuts at: 0 1762 3984 Size: 1762 2222 BamHI G'GATC_C Cuts at: 0 819 3984 Size: 819 3165 BanI G'GyrC_C Cuts at: 0 1443 3652 3984 Size: 1443 2209 332 Fragments arranged by size: 2209 1443 332 BanII G_rGCy'C Cuts at: 0 392 455 773 801 3089 3984 Size: 392 63 318 28 2288 895 Fragments arranged by size: 2288 895 392 318 63 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 302 827 1645 2303 2564 3984 Size: 302 525 818 658 261 1420 Fragments arranged by size: 1420 818 658 525 302 261 BciVI GTATCCnnnnn_n' Cuts at: 0 3273 3984 Size: 3273 711 BfrBI ATG'CAT Cuts at: 0 1692 3681 3984 Size: 1692 1989 303 Fragments arranged by size: 1989 1692 303 BglII A'GATC_T Cuts at: 0 487 1926 2505 3984 Size: 487 1439 579 1479 Fragments arranged by size: 1479 1439 579 487 BlpI GC'TnA_GC Cuts at: 0 772 3984 Size: 772 3212 Bme1580I G_kGCm'C Cuts at: 0 345 2576 3984 Size: 345 2231 1408 Fragments arranged by size: 2231 1408 345 BmrI ACTGGGnnnn_n' Cuts at: 0 191 2570 3984 Size: 191 2379 1414 Fragments arranged by size: 2379 1414 191 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 3771 3931 3984 Size: 925 2846 160 53 Fragments arranged by size: 2846 925 160 53 Bpu10I CC'TnA_GC Cuts at: 0 391 737 2375 3088 3984 Size: 391 346 1638 713 896 Fragments arranged by size: 1638 896 713 391 346 BsaI GGTCTCn'nnnn_ Cuts at: 0 3238 3984 Size: 3238 746 BsaAI yAC'GTr Cuts at: 0 2048 3984 Size: 2048 1936 BsaWI w'CCGG_w Cuts at: 0 1811 3314 3984 Size: 1811 1503 670 Fragments arranged by size: 1811 1503 670 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 788 818 1496 1526 3009 3039 3744 3774 Size: 788 30 678 30 1483 30 705 30 Cuts at: 3774 3984 Size: 210 Fragments arranged by size: 1483 788 705 678 210 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 527 3121 3984 Size: 151 376 2594 863 Fragments arranged by size: 2594 863 376 151 BseYI C'CCAG_C Cuts at: 0 1190 2564 3984 Size: 1190 1374 1420 Fragments arranged by size: 1420 1374 1190 BsiHKAI G_wGCw'C Cuts at: 0 392 455 773 871 2207 3843 3984 Size: 392 63 318 98 1336 1636 141 Fragments arranged by size: 1636 1336 392 318 141 98 63 BsmI GAATG_Cn' Cuts at: 0 1194 3984 Size: 1194 2790 Bsp1286I G_dGCh'C Cuts at: 0 345 392 455 773 801 871 2207 2576 Size: 345 47 63 318 28 70 1336 369 Cuts at: 2576 3089 3843 3984 Size: 513 754 141 Fragments arranged by size: 1336 754 513 369 345 318 141 70 63 47 28 BspHI T'CATG_A Cuts at: 0 54 2784 3984 Size: 54 2730 1200 Fragments arranged by size: 2730 1200 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3631 3984 Size: 3631 353 BsrDI GCAATG_nn' Cuts at: 0 37 465 1286 3469 3984 Size: 37 428 821 2183 515 Fragments arranged by size: 2183 821 515 428 37 BsrGI T'GTAC_A Cuts at: 0 1563 2244 2601 3984 Size: 1563 681 357 1383 Fragments arranged by size: 1563 1383 681 357 BstXI CCAn_nnnn'nTGG Cuts at: 0 2361 3630 3984 Size: 2361 1269 354 Fragments arranged by size: 2361 1269 354 BstYI r'GATC_y Cuts at: 0 192 487 819 1146 1926 2505 2537 2985 Size: 192 295 332 327 780 579 32 448 Cuts at: 2985 3984 Size: 999 Fragments arranged by size: 999 780 579 448 332 327 295 192 32 BstZ17I GTA'TAC Cuts at: 0 3225 3984 Size: 3225 759 BtgI C'CryG_G Cuts at: 0 1053 3806 3984 Size: 1053 2753 178 Fragments arranged by size: 2753 1053 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 311 3535 3984 Size: 311 3224 449 Fragments arranged by size: 3224 449 311 BtsI GCAGTG_nn' Cuts at: 0 2576 3984 Size: 2576 1408 DraI TTT'AAA Cuts at: 0 3158 3647 3984 Size: 3158 489 337 Fragments arranged by size: 3158 489 337 DraIII CAC_nnn'GTG Cuts at: 0 2116 3984 Size: 2116 1868 DrdI GACnn_nn'nnGTC Cuts at: 0 831 3984 Size: 831 3153 EaeI y'GGCC_r Cuts at: 0 603 3818 3984 Size: 603 3215 166 Fragments arranged by size: 3215 603 166 EarI CTCTTCn'nnn_ Cuts at: 0 72 566 1031 1076 1217 1298 2119 2441 Size: 72 494 465 45 141 81 821 322 Cuts at: 2441 2516 3984 Size: 75 1468 Fragments arranged by size: 1468 821 494 465 322 141 81 75 72 45 EcoICRI GAG'CTC Cuts at: 0 390 453 771 3984 Size: 390 63 318 3213 Fragments arranged by size: 3213 390 318 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 590 925 1097 1688 2297 2537 3185 Size: 568 22 335 172 591 609 240 648 Cuts at: 3185 3771 3931 3976 3984 Size: 586 160 45 8 Fragments arranged by size: 648 609 591 586 568 335 240 172 160 45 22 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 907 3984 Size: 907 3077 EcoO109I rG'GnC_Cy Cuts at: 0 1672 2000 3984 Size: 1672 328 1984 Fragments arranged by size: 1984 1672 328 EcoRI G'AATT_C Cuts at: 0 1593 2398 2586 3984 Size: 1593 805 188 1398 Fragments arranged by size: 1593 1398 805 188 EcoRV GAT'ATC Cuts at: 0 3558 3747 3984 Size: 3558 189 237 Fragments arranged by size: 3558 237 189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2762 3981 3984 Size: 2762 1219 3 Fragments arranged by size: 2762 1219 3 FspI TGC'GCA Cuts at: 0 3904 3984 Size: 3904 80 FspAI rTGC'GCAy Cuts at: 0 3904 3984 Size: 3904 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 49 81 644 676 873 905 1431 1463 Size: 49 32 563 32 197 32 526 32 Cuts at: 1463 1967 1999 2314 2346 2941 2973 3862 3894 Size: 504 32 315 32 595 32 889 32 Cuts at: 3894 3984 Size: 90 Fragments arranged by size: 889 595 563 526 504 315 197 90 49 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1554 3363 3984 Size: 1554 1809 621 Fragments arranged by size: 1809 1554 621 HindIII A'AGCT_T Cuts at: 0 1323 3984 Size: 1323 2661 MfeI C'AATT_G Cuts at: 0 1756 3984 Size: 1756 2228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 97 847 1592 3984 Size: 97 750 745 2392 Fragments arranged by size: 2392 750 745 97 MslI CAynn'nnrTG Cuts at: 0 368 1271 3080 3415 3984 Size: 368 903 1809 335 569 Fragments arranged by size: 1809 903 569 368 335 MspA1I CmG'CkG Cuts at: 0 2564 3984 Size: 2564 1420 NcoI C'CATG_G Cuts at: 0 1053 3806 3984 Size: 1053 2753 178 Fragments arranged by size: 2753 1053 178 NdeI CA'TA_TG Cuts at: 0 590 2165 3984 Size: 590 1575 1819 Fragments arranged by size: 1819 1575 590 NruI TCG'CGA Cuts at: 0 3554 3984 Size: 3554 430 NsiI A_TGCA'T Cuts at: 0 1694 3683 3984 Size: 1694 1989 301 Fragments arranged by size: 1989 1694 301 NspI r_CATG'y Cuts at: 0 31 355 867 3361 3389 3669 3909 3984 Size: 31 324 512 2494 28 280 240 75 Fragments arranged by size: 2494 512 324 280 240 75 31 28 PciI A'CATG_T Cuts at: 0 863 3357 3984 Size: 863 2494 627 Fragments arranged by size: 2494 863 627 PflMI CCAn_nnn'nTGG Cuts at: 0 1751 2970 3984 Size: 1751 1219 1014 Fragments arranged by size: 1751 1219 1014 PfoI T'CCnGG_A Cuts at: 0 792 1860 3984 Size: 792 1068 2124 Fragments arranged by size: 2124 1068 792 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3469 3501 3984 Size: 3469 32 483 Fragments arranged by size: 3469 483 32 PpuMI rG'GwC_Cy Cuts at: 0 1672 2000 3984 Size: 1672 328 1984 Fragments arranged by size: 1984 1672 328 PshAI GACnn'nnGTC Cuts at: 0 50 161 3984 Size: 50 111 3823 Fragments arranged by size: 3823 111 50 PsiI TTA'TAA Cuts at: 0 3454 3984 Size: 3454 530 PstI C_TGCA'G Cuts at: 0 163 1029 2925 3984 Size: 163 866 1896 1059 Fragments arranged by size: 1896 1059 866 163 PvuII CAG'CTG Cuts at: 0 2564 3984 Size: 2564 1420 SacI G_AGCT'C Cuts at: 0 392 455 773 3984 Size: 392 63 318 3211 Fragments arranged by size: 3211 392 318 63 SanDI GG'GwC_CC Cuts at: 0 2000 3984 Size: 2000 1984 SapI GCTCTTCn'nnn_ Cuts at: 0 2119 2516 3984 Size: 2119 397 1468 Fragments arranged by size: 2119 1468 397 SfcI C'TryA_G Cuts at: 0 159 1025 2921 3322 3782 3984 Size: 159 866 1896 401 460 202 Fragments arranged by size: 1896 866 460 401 202 159 SmlI C'TyrA_G Cuts at: 0 1930 2835 3670 3984 Size: 1930 905 835 314 Fragments arranged by size: 1930 905 835 314 SpeI A'CTAG_T Cuts at: 0 3238 3984 Size: 3238 746 SspI AAT'ATT Cuts at: 0 1020 1296 2764 3984 Size: 1020 276 1468 1220 Fragments arranged by size: 1468 1220 1020 276 StuI AGG'CCT Cuts at: 0 1749 3984 Size: 1749 2235 StyI C'CwwG_G Cuts at: 0 929 1053 1484 1750 1762 2237 3185 3806 Size: 929 124 431 266 12 475 948 621 Cuts at: 3806 3984 Size: 178 Fragments arranged by size: 948 929 621 475 431 266 178 124 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3670 3984 Size: 3670 314 TatI w'GTAC_w Cuts at: 0 596 1309 1314 1563 2244 2601 3763 3984 Size: 596 713 5 249 681 357 1162 221 Fragments arranged by size: 1162 713 681 596 357 249 221 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 219 251 1703 1735 3174 3206 3984 Size: 219 32 1452 32 1439 32 778 Fragments arranged by size: 1452 1439 778 219 32 32 32 XbaI T'CTAG_A Cuts at: 0 2670 3984 Size: 2670 1314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3813 3984 Size: 3813 171 XmnI GAAnn'nnTTC Cuts at: 0 2398 3984 Size: 2398 1586 Enzymes that do cut: AccI AcuI AflII AflIII AlwNI ApoI AvrII BamHI BanI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NdeI NruI NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PstI PvuII SacI SanDI SapI SfcI SmlI SpeI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI BbvCI BcgI BclI BglI BmgBI BmtI BplI BpuEI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII Bsu36I ClaI CspCI EagI EciI FseI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NgoMIV NheI NotI PacI PasI PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I XhoI XmaI ZraI