################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccI AflII AgeI AhdI AlwI AocI AscI AseI AsnI AspEI AvrII BamHI BbrPI BbvCI BclI BfrI BglII BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI DraI DraIII Eam1105I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI MamI MfeI MflI MluI MunI NdeI NheI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI SacI SalI SapI SbfI ScaI SexAI SfcI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp45I XhoI XhoII ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 895 GGTACC G^GTACC AclI 2153 AACGTT AA^CGTT AflIII 1280 ACGTGT A^C[GA][CT]GT AlwNI 769 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 895 GGTACC G^GTACC AspI 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 933 GTCTTC GAAGAC(2/6) BciVI 2163 GGATAC GTATCC(6/5) BlpI 1062 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1062 GCTGAGC GC^T[ACGT]AGC BpuAI 933 GTCTTC GAAGAC(2/6) BsaAI 2015 CACGTA [CT]AC^GT[GA] CelII 1062 GCTGAGC GC^T[ACGT]AGC CpoI 1851 CGGTCCG CG^G[AT]CCG DrdI 939 GACCTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 942 CTCTTC CTCTTC(1/4) EcoRI 142 GAATTC G^AATTC HincII 115 GTCAAC GT[CT]^[GA]AC HindII 115 GTCAAC GT[CT]^[GA]AC KpnI 895 GGTACC GGTAC^C Ksp632I 942 CTCTTC CTCTTC(1/4) NaeI 1980 GCCGGC GCC^GGC NcoI 2111 CCATGG C^CATGG NgoMIV 1980 GCCGGC G^CCGGC NotI 286 GCGGCCGC GC^GGCCGC NruI 2333 TCGCGA TCG^CGA PflFI 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2153 AACGTT AA^CGTT RsrII 1851 CGGTCCG CG^G[AT]CCG SphI 62 GCATGC GCATG^C StuI 1628 AGGCCT AGG^CCT TfiI 2104 GATTC G^A[AT]TC Tth111I 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2221 TCTAGA T^CTAGA XcmI 1220 CCAAGCCCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 1635 TCCGGA T^CCGGA 2100 TCCGGA Alw44I 1930 GTGCAC G^TGCAC 2083 GTGCAC ApaLI 1930 GTGCAC G^TGCAC 2083 GTGCAC Asp700I 1245 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1332 GAAGGACTTC AviII 1076 TGCGCA TGC^GCA 1616 TGCGCA BalI 1304 TGGCCA TGG^CCA 2114 TGGCCA BcgI 137 GCATCGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 497 GCAGCCGCTTCG BsaI 1418 GAGACC GGTCTC(1/5) 2375 GAGACC BseAI 1635 TCCGGA T^CCGGA 2100 TCCGGA BseRI 607 GAGGAG GAGGAG(10/8) 1090 GAGGAG BsgI 1146 CTGCAC GTGCAG(16/14) 2136 CTGCAC BsmBI 671 CGTCTC CGTCTC(1/5) 1215 CGTCTC BspEI 1635 TCCGGA T^CCGGA 2100 TCCGGA BsrBI 201 CCGCTC CCGCTC(-3/-3) 1868 CCGCTC BssHII 160 GCGCGC G^CGCGC 1661 GCGCGC BstAPI 768 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2138 GCACCATTTGC FspI 1076 TGCGCA TGC^GCA 1616 TGCGCA KspI 105 CCGCGG CCGC^GG 482 CCGCGG MaeIII 700 GTTAC ^GT[ACGT]AC 2018 GTAAC MluNI 1304 TGGCCA TGG^CCA 2114 TGGCCA MroI 1635 TCCGGA T^CCGGA 2100 TCCGGA MscI 1304 TGGCCA TGG^CCA 2114 TGGCCA MseI 779 TTAA T^TAA 2357 TTAA MslI 382 CACGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1875 CATGTCGGTG NspI 62 GCATGC [GA]CATG^[CT] 1472 ACATGC PflMI 74 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 822 CCACGCCCTGG PvuII 166 CAGCTG CAG^CTG 1500 CAGCTG SacII 105 CCGCGG CCGC^GG 482 CCGCGG SfiI 568 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2238 GGCCCAGGGGGCC SgrAI 521 CGCCGGTG C[GA]^CCGG[CT]G 2250 CACCGGCG Tru9I 779 TTAA T^TAA 2357 TTAA Van91I 74 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 822 CCACGCCCTGG XmnI 1245 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1332 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Aor51HI ApaI ApoI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsaWI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI StyI TaqI TseI Tsp509I TspRI TthHB8I XmaCI XmaI