(Circular) (Six-base) MAPSORT of: pFN21AE1282.seq Check: 4969 from: 1 to: 7087 pFN21AE1282 7087 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2008 2008 Size: 7087 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4897 278 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4430 1937 Size: 2493 4594 Acc65I G'GTAC_C Cuts at: 2900 4408 2900 Size: 1508 5579 AclI AA'CG_TT Cuts at: 1563 4159 5214 5587 1563 Size: 2596 1055 373 3063 Fragments arranged by size: 3063 2596 1055 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2640 3692 3749 5150 6534 6802 1632 Size: 563 445 1052 57 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1052 563 445 268 57 AfeI AGC'GCT Cuts at: 2441 2455 2800 2441 Size: 14 345 6728 Fragments arranged by size: 6728 345 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6890 AflIII A'CryG_T Cuts at: 1203 3285 5986 6963 1203 Size: 2082 2701 977 1327 Fragments arranged by size: 2701 2082 1327 977 AgeI A'CCGG_T Cuts at: 5918 5918 Size: 7087 AhdI GACnn_n'nnGTC Cuts at: 5816 5816 Size: 7087 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3950 1184 Size: 2766 4321 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3845 3877 3845 Size: 32 7055 AlwNI CAG_nnn'CTG Cuts at: 2779 4830 6402 2779 Size: 2051 1572 3464 Fragments arranged by size: 3464 2051 1572 ApaI G_GGCC'C Cuts at: 2585 2850 2885 2585 Size: 265 35 6787 Fragments arranged by size: 6787 265 35 ApaLI G'TGCA_C Cuts at: 3935 4088 5144 6300 3935 Size: 153 1056 1156 4722 Fragments arranged by size: 4722 1156 1056 153 ApoI r'AATT_y Cuts at: 1493 2147 4036 4208 4396 4622 4774 6883 1493 Size: 654 1889 172 188 226 152 2109 1697 Fragments arranged by size: 2109 1889 1697 654 226 188 172 152 AseI AT'TA_AT Cuts at: 160 1032 5641 160 Size: 872 4609 1606 Fragments arranged by size: 4609 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2722 2894 2905 3277 3361 3562 Size: 73 270 509 172 11 372 84 201 Cuts at: 3562 4072 4412 1870 Size: 510 340 4545 Fragments arranged by size: 4545 510 509 372 340 270 201 172 84 73 11 BamHI G'GATC_C Cuts at: 4417 4784 4417 Size: 367 6720 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2900 3411 3470 3760 4048 Size: 325 217 627 1113 511 59 290 288 Cuts at: 4048 4408 5863 618 Size: 360 1455 1842 Fragments arranged by size: 1842 1455 1113 627 511 360 325 290 288 217 59 BanII G_rGCy'C Cuts at: 1391 2585 2645 2850 2875 2885 3278 3648 4406 Size: 1194 60 205 25 10 393 370 758 Cuts at: 4406 1391 Size: 4072 Fragments arranged by size: 4072 1194 758 393 370 205 60 25 10 BbeI G_GCGC'C Cuts at: 3415 3474 3764 4052 3415 Size: 59 290 288 6450 Fragments arranged by size: 6450 290 288 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2931 928 Size: 971 1032 5084 Fragments arranged by size: 5084 1032 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2131 2165 2491 2525 5278 5312 1903 Size: 34 194 34 326 34 2753 34 3678 Fragments arranged by size: 3678 2753 326 194 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4162 4979 6189 4162 Size: 817 1210 5060 Fragments arranged by size: 5060 1210 817 BclI T'GATC_A Cuts at: 1548 1548 Size: 7087 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2255 2580 Size: 107 122 71 631 605 337 246 325 Cuts at: 2580 2589 4250 5698 136 Size: 9 1661 1448 1525 Fragments arranged by size: 1661 1525 1448 631 605 337 325 246 122 107 71 9 BglII A'GATC_T Cuts at: 7082 7082 Size: 7087 BlpI GC'TnA_GC Cuts at: 3068 4504 3068 Size: 1436 5651 Bme1580I G_kGCm'C Cuts at: 1790 2585 2757 2850 2885 3468 3771 3905 3939 Size: 795 172 93 35 583 303 134 34 Cuts at: 3939 4092 4190 5148 6304 1790 Size: 153 98 958 1156 2573 Fragments arranged by size: 2573 1156 958 795 583 303 172 153 134 98 93 35 34 BmgBI CAC'GTC Cuts at: 1318 2922 1318 Size: 1604 5483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3971 4818 5776 Size: 416 53 83 399 24 2554 847 958 Cuts at: 5776 442 Size: 1753 Fragments arranged by size: 2554 1753 958 847 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7087 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3446 Size: 7087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 3277 3398 5747 1239 Size: 1217 821 121 2349 2579 Fragments arranged by size: 2579 2349 1217 821 121 Bpu10I CC'TnA_GC Cuts at: 2259 2450 2259 Size: 191 6896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5212 6077 6375 5212 Size: 865 298 5924 Fragments arranged by size: 5924 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3417 4374 5750 882 Size: 617 1918 957 1376 2219 Fragments arranged by size: 2219 1918 1376 957 617 BsaAI yAC'GTr Cuts at: 493 1204 4022 4878 493 Size: 711 2818 856 2702 Fragments arranged by size: 2818 2702 856 711 BsaBI GATnn'nnATC Cuts at: 7081 7081 Size: 7087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2666 2675 2855 3216 Size: 53 83 186 936 1133 9 180 361 Cuts at: 3216 3412 3471 3501 3761 4049 4894 5276 5911 Size: 196 59 30 260 288 845 382 635 Cuts at: 5911 275 Size: 1451 Fragments arranged by size: 1451 1133 936 845 635 382 361 288 260 196 186 180 83 59 53 30 9 BsaWI w'CCGG_w Cuts at: 3640 3822 4105 5520 5918 6192 6339 6750 3640 Size: 182 283 1415 398 274 147 411 3977 Fragments arranged by size: 3977 1415 411 398 283 274 182 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2845 2875 3011 Size: 30 165 30 789 30 623 30 136 Cuts at: 3011 3041 3206 3236 3275 3305 1178 Size: 30 165 30 39 30 4960 Fragments arranged by size: 4960 789 623 165 165 136 39 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 3110 1186 Size: 1441 483 5163 Fragments arranged by size: 5163 1441 483 BseYI C'CCAG_C Cuts at: 2742 3193 3486 3551 3676 4199 4274 4352 6290 Size: 451 293 65 125 523 75 78 1938 Cuts at: 6290 6786 2742 Size: 496 3043 Fragments arranged by size: 3043 1938 523 496 451 293 125 78 75 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3136 4126 1163 Size: 573 1400 990 4124 Fragments arranged by size: 4124 1400 990 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 2295 3349 4537 5298 6326 Size: 275 186 205 89 1054 1188 761 1028 Cuts at: 6326 1540 Size: 2301 Fragments arranged by size: 2301 1188 1054 1028 761 275 205 186 89 BsiHKAI G_wGCw'C Cuts at: 3011 3939 4092 5148 5233 6304 3011 Size: 928 153 1056 85 1071 3794 Fragments arranged by size: 3794 1071 1056 928 153 85 BsmI GAATG_Cn' Cuts at: 4598 4691 4598 Size: 93 6994 BsmBI CGTCTCn'nnnn_ Cuts at: 2682 3226 2682 Size: 544 6543 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2585 2645 2757 2850 2875 2885 3011 Size: 399 795 60 112 93 25 10 126 Cuts at: 3011 3278 3468 3648 3771 3905 3939 4092 4190 Size: 267 190 180 123 134 34 153 98 Cuts at: 4190 4406 5148 5233 6304 1391 Size: 216 742 85 1071 2174 Fragments arranged by size: 2174 1071 795 742 399 267 216 190 180 153 134 126 123 112 98 93 85 60 34 25 10 BspEI T'CCGG_A Cuts at: 3640 4105 6750 3640 Size: 465 2645 3977 Fragments arranged by size: 3977 2645 465 BspHI T'CATG_A Cuts at: 4976 6706 4976 Size: 1730 5357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 2067 2772 3435 4442 844 Size: 1164 59 705 663 1007 3489 Fragments arranged by size: 3489 1164 1007 705 663 59 BsrBI CCG'CTC Cuts at: 1394 1823 2208 3875 4974 1394 Size: 429 385 1667 1099 3507 Fragments arranged by size: 3507 1667 1099 429 385 BsrDI GCAATG_nn' Cuts at: 65 1248 5582 5756 65 Size: 1183 4334 174 1396 Fragments arranged by size: 4334 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2527 3985 4256 5731 5918 1700 Size: 110 717 1458 271 1475 187 2869 Fragments arranged by size: 2869 1475 1458 717 271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7087 BssHII G'CGCG_C Cuts at: 1925 2165 3666 1925 Size: 240 1501 5346 Fragments arranged by size: 5346 1501 240 BssSI C'ACGA_G Cuts at: 5147 6159 5147 Size: 1012 6075 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 4149 794 Size: 1985 1370 3732 Fragments arranged by size: 3732 1985 1370 BstBI TT'CG_AA Cuts at: 5891 5891 Size: 7087 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7087 BstXI CCAn_nnnn'nTGG Cuts at: 2393 2575 3529 2393 Size: 182 954 5951 Fragments arranged by size: 5951 954 182 BstYI r'GATC_y Cuts at: 1972 4417 4784 5169 5186 6638 6724 6736 7082 Size: 2445 367 385 17 1452 86 12 346 Cuts at: 7082 1972 Size: 1977 Fragments arranged by size: 2445 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2110 2487 2842 3270 4116 513 Size: 552 1045 377 355 428 846 3484 Fragments arranged by size: 3484 1045 846 552 428 377 355 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6348 Fragments arranged by size: 6348 683 56 BtsI GCAGTG_nn' Cuts at: 3522 3707 3945 4603 5397 5417 3522 Size: 185 238 658 794 20 5192 Fragments arranged by size: 5192 794 658 238 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7087 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4193 4228 529 Size: 35 3629 35 3388 Fragments arranged by size: 3629 3388 35 35 DraI TTT'AAA Cuts at: 4391 4391 Size: 7087 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2950 6094 809 Size: 507 1634 3144 1802 Fragments arranged by size: 3144 1802 1634 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2292 2793 Size: 54 1425 50 275 18 373 89 501 Cuts at: 2793 2916 3060 3309 3346 3870 4119 4534 5423 Size: 123 144 249 37 524 249 415 889 Cuts at: 5423 7052 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 524 501 415 373 275 249 249 144 123 89 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2203 2292 3346 4534 1537 Size: 275 391 89 1054 1188 4090 Fragments arranged by size: 4090 1188 1054 391 275 89 EarI CTCTTCn'nnn_ Cuts at: 1353 2953 5017 1353 Size: 1600 2064 3423 Fragments arranged by size: 3423 2064 1600 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2748 3733 3774 3780 5674 6048 Size: 1064 138 1159 985 41 6 1894 374 Cuts at: 6048 6194 387 Size: 146 1280 Fragments arranged by size: 1894 1280 1159 1064 985 374 146 138 41 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2456 2640 3277 3398 3692 3749 Size: 393 563 261 184 637 121 294 57 Cuts at: 3749 5150 5747 6534 6802 1239 Size: 1401 597 787 268 1524 Fragments arranged by size: 1524 1401 787 637 597 563 393 294 268 261 184 121 57 EcoO109I rG'GnC_Cy Cuts at: 2581 2618 2738 2847 2881 2882 3055 3352 3573 Size: 37 120 109 34 1 173 297 221 Cuts at: 3573 3809 4030 2581 Size: 236 221 5638 Fragments arranged by size: 5638 297 236 221 221 173 120 109 37 34 1 EcoRI G'AATT_C Cuts at: 2147 4396 4774 2147 Size: 2249 378 4460 Fragments arranged by size: 4460 2249 378 FspI TGC'GCA Cuts at: 3083 3623 5593 3083 Size: 540 1970 4577 Fragments arranged by size: 4577 1970 540 HaeII r_GCGC'y Cuts at: 1678 2136 2443 2457 2802 3415 3474 3728 3764 Size: 458 307 14 345 613 59 254 36 Cuts at: 3764 4009 4052 4068 6234 1678 Size: 245 43 16 2166 2531 Fragments arranged by size: 2531 2166 613 458 345 307 254 245 59 43 36 16 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2845 2877 3206 Size: 32 135 32 620 32 621 32 329 Cuts at: 3206 3238 4221 4253 5728 5760 5802 5834 1373 Size: 32 983 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 983 621 620 329 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 4431 4677 669 Size: 988 281 184 2309 246 3079 Fragments arranged by size: 3079 2309 988 281 246 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 7087 HpaI GTT'AAC Cuts at: 4677 4677 Size: 7087 KasI G'GCGC_C Cuts at: 3411 3470 3760 4048 3411 Size: 59 290 288 6450 Fragments arranged by size: 6450 290 288 59 KpnI G_GTAC'C Cuts at: 2904 4412 2904 Size: 1508 5579 MfeI C'AATT_G Cuts at: 4686 4686 Size: 7087 MluI A'CGCG_T Cuts at: 6963 6963 Size: 7087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3843 6201 6385 1305 Size: 2538 2358 184 2007 Fragments arranged by size: 2538 2358 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3311 4121 10 Size: 54 1425 343 1479 810 2976 Fragments arranged by size: 2976 1479 1425 810 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 3884 5045 5404 5563 518 Size: 648 1225 1493 1161 359 159 2042 Fragments arranged by size: 2042 1493 1225 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2173 2288 2489 2660 3507 4128 4278 Size: 506 61 115 201 171 847 621 150 Cuts at: 4278 4503 5180 6328 6573 1606 Size: 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 621 506 245 225 201 171 150 115 61 NaeI GCC'GGC Cuts at: 3987 3987 Size: 7087 NarI GG'CG_CC Cuts at: 3412 3471 3761 4049 3412 Size: 59 290 288 6450 Fragments arranged by size: 6450 290 288 59 NcoI C'CATG_G Cuts at: 513 1065 4116 513 Size: 552 3051 3484 Fragments arranged by size: 3484 3051 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7087 NgoMIV G'CCGG_C Cuts at: 3985 3985 Size: 7087 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7087 NotI GC'GGCC_GC Cuts at: 2292 4534 2292 Size: 2242 4845 NruI TCG'CGA Cuts at: 4340 4340 Size: 7087 NspI r_CATG'y Cuts at: 1132 1229 2071 3481 4445 5990 1132 Size: 97 842 1410 964 1545 2229 Fragments arranged by size: 2229 1545 1410 964 842 97 PasI CC'CwG_GG Cuts at: 1348 2073 2833 2867 4246 1348 Size: 725 760 34 1379 4189 Fragments arranged by size: 4189 1379 760 725 34 PciI A'CATG_T Cuts at: 5986 5986 Size: 7087 PflMI CCAn_nnn'nTGG Cuts at: 1108 2085 2833 1108 Size: 977 748 5362 Fragments arranged by size: 5362 977 748 PfoI T'CCnGG_A Cuts at: 2030 2196 2030 Size: 166 6921 PmeI GTTT'AAAC Cuts at: 4391 4391 Size: 7087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5156 5188 6669 6701 5156 Size: 32 1481 32 5542 Fragments arranged by size: 5542 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2618 3809 4030 2618 Size: 1191 221 5675 Fragments arranged by size: 5675 1191 221 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7087 PsiI TTA'TAA Cuts at: 4657 4657 Size: 7087 PspOMI G'GGCC_C Cuts at: 2581 2846 2881 2581 Size: 265 35 6787 Fragments arranged by size: 6787 265 35 PstI C_TGCA'G Cuts at: 830 4439 830 Size: 3609 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7087 PvuII CAG'CTG Cuts at: 2173 3507 2173 Size: 1334 5753 RsrII CG'GwC_CG Cuts at: 3857 3857 Size: 7087 SacII CC_GC'GG Cuts at: 2113 2490 2113 Size: 377 6710 SalI G'TCGA_C Cuts at: 1936 4429 1936 Size: 2493 4594 SanDI GG'GwC_CC Cuts at: 2618 3809 2618 Size: 1191 5896 SbfI CC_TGCA'GG Cuts at: 4439 4439 Size: 7087 ScaI AGT'ACT Cuts at: 5335 7047 5335 Size: 1712 5375 SfcI C'TryA_G Cuts at: 826 1045 4435 5570 6251 6442 826 Size: 219 3390 1135 681 191 1471 Fragments arranged by size: 3390 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2580 4250 2580 Size: 1670 5417 SfoI GGC'GCC Cuts at: 3413 3472 3762 4050 3413 Size: 59 290 288 6450 Fragments arranged by size: 6450 290 288 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 SgrAI Cr'CCGG_yG Cuts at: 1700 2527 4256 1700 Size: 827 1729 4531 Fragments arranged by size: 4531 1729 827 SmaI CCC'GGG Cuts at: 1872 2215 2724 2907 3564 4074 4414 1872 Size: 343 509 183 657 510 340 4545 Fragments arranged by size: 4545 657 510 509 343 340 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 5191 6092 6354 820 Size: 197 926 3248 901 262 1553 Fragments arranged by size: 3248 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4878 493 Size: 4385 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7087 SphI G_CATG'C Cuts at: 1132 2071 4445 1132 Size: 939 2374 3774 Fragments arranged by size: 3774 2374 939 SspI AAT'ATT Cuts at: 5 52 5011 5 Size: 47 4959 2081 Fragments arranged by size: 4959 2081 47 StuI AGG'CCT Cuts at: 1517 3635 1517 Size: 2118 4969 StyI C'CwwG_G Cuts at: 513 1065 3918 4116 4313 4526 513 Size: 552 2853 198 197 213 3074 Fragments arranged by size: 3074 2853 552 213 198 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2963 5114 5131 5284 5469 5914 325 Size: 1255 1383 2151 17 153 185 445 1498 Fragments arranged by size: 2151 1498 1383 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3288 5333 7045 Size: 274 80 33 51 1443 1311 2045 1712 Cuts at: 7045 96 Size: 138 Fragments arranged by size: 2045 1712 1443 1311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7047 7079 7047 Size: 32 7055 Tth111I GACn'n_nGTC Cuts at: 1316 3218 1316 Size: 1902 5185 XbaI T'CTAG_A Cuts at: 4226 4423 4226 Size: 197 6890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3232 3232 Size: 7087 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7087 XmaI C'CCGG_G Cuts at: 1870 2213 2722 2905 3562 4072 4412 1870 Size: 343 509 183 657 510 340 4545 Fragments arranged by size: 4545 657 510 509 343 340 183 XmnI GAAnn'nnTTC Cuts at: 3254 3341 5216 3254 Size: 87 1875 5125 Fragments arranged by size: 5125 1875 87 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4895 276 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PmlI PspXI PsrI SacI SapI SexAI SrfI SwaI