(Linear) (Six-base) MAPSORT of: KIEE1282.seq Check: 6920 from: 1 to: 2382 KIEE1282.orf With 182 enzymes: SgfI * July 3, 2012 16:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3 2382 Size: 3 2379 Acc65I G'GTAC_C Cuts at: 0 895 2382 Size: 895 1487 AclI AA'CG_TT Cuts at: 0 2154 2382 Size: 2154 228 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 635 1687 1744 2382 Size: 190 445 1052 57 638 Fragments arranged by size: 1052 638 445 190 57 AfeI AGC'GCT Cuts at: 0 436 450 795 2382 Size: 436 14 345 1587 Fragments arranged by size: 1587 436 345 14 AflIII A'CryG_T Cuts at: 0 1280 2382 Size: 1280 1102 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1945 2382 Size: 1945 437 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1840 1872 2382 Size: 1840 32 510 Fragments arranged by size: 1840 510 32 AlwNI CAG_nnn'CTG Cuts at: 0 774 2382 Size: 774 1608 ApaI G_GGCC'C Cuts at: 0 580 845 880 2382 Size: 580 265 35 1502 Fragments arranged by size: 1502 580 265 35 ApaLI G'TGCA_C Cuts at: 0 1930 2083 2382 Size: 1930 153 299 Fragments arranged by size: 1930 299 153 ApoI r'AATT_y Cuts at: 0 142 2031 2203 2382 Size: 142 1889 172 179 Fragments arranged by size: 1889 179 172 142 AvaI C'yCGr_G Cuts at: 0 208 717 889 900 1272 1356 1557 2067 Size: 208 509 172 11 372 84 201 510 Cuts at: 2067 2382 Size: 315 Fragments arranged by size: 510 509 372 315 208 201 172 84 11 BanI G'GyrC_C Cuts at: 0 895 1406 1465 1755 2043 2382 Size: 895 511 59 290 288 339 Fragments arranged by size: 895 511 339 290 288 59 BanII G_rGCy'C Cuts at: 0 580 640 845 870 880 1273 1643 2382 Size: 580 60 205 25 10 393 370 739 Fragments arranged by size: 739 580 393 370 205 60 25 10 BbeI G_GCGC'C Cuts at: 0 1410 1469 1759 2047 2382 Size: 1410 59 290 288 335 Fragments arranged by size: 1410 335 290 288 59 BbsI GAAGACnn'nnnn_ Cuts at: 0 926 2382 Size: 926 1456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 126 160 486 520 2382 Size: 126 34 326 34 1862 Fragments arranged by size: 1862 326 126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2157 2382 Size: 2157 225 BglI GCCn_nnn'nGGC Cuts at: 0 250 575 584 2245 2382 Size: 250 325 9 1661 137 Fragments arranged by size: 1661 325 250 137 9 BlpI GC'TnA_GC Cuts at: 0 1063 2382 Size: 1063 1319 Bme1580I G_kGCm'C Cuts at: 0 580 752 845 880 1463 1766 1900 1934 Size: 580 172 93 35 583 303 134 34 Cuts at: 1934 2087 2185 2382 Size: 153 98 197 Fragments arranged by size: 583 580 303 197 172 153 134 98 93 35 34 BmgBI CAC'GTC Cuts at: 0 917 2382 Size: 917 1465 BmrI ACTGGGnnnn_n' Cuts at: 0 1966 2382 Size: 1966 416 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1441 2382 Size: 1441 941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 1272 1393 2382 Size: 451 821 121 989 Fragments arranged by size: 989 821 451 121 Bpu10I CC'TnA_GC Cuts at: 0 254 445 2382 Size: 254 191 1937 Fragments arranged by size: 1937 254 191 BsaI GGTCTCn'nnnn_ Cuts at: 0 1412 2369 2382 Size: 1412 957 13 Fragments arranged by size: 1412 957 13 BsaAI yAC'GTr Cuts at: 0 2017 2382 Size: 2017 365 BsaHI Gr'CG_yC Cuts at: 0 661 670 850 1211 1407 1466 1496 1756 Size: 661 9 180 361 196 59 30 260 Cuts at: 1756 2044 2382 Size: 288 338 Fragments arranged by size: 661 361 338 288 260 196 180 59 30 9 BsaWI w'CCGG_w Cuts at: 0 1635 1817 2100 2382 Size: 1635 182 283 282 Fragments arranged by size: 1635 283 282 182 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 187 217 840 870 1006 1036 1201 1231 Size: 187 30 623 30 136 30 165 30 Cuts at: 1231 1270 1300 2382 Size: 39 30 1082 Fragments arranged by size: 1082 623 187 165 136 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 622 1105 2382 Size: 622 483 1277 Fragments arranged by size: 1277 622 483 BseYI C'CCAG_C Cuts at: 0 737 1188 1481 1546 1671 2194 2269 2347 Size: 737 451 293 65 125 523 75 78 Cuts at: 2347 2382 Size: 35 Fragments arranged by size: 737 523 451 293 125 78 75 65 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1131 2121 2382 Size: 1131 990 261 Fragments arranged by size: 1131 990 261 BsiEI CG_ry'CG Cuts at: 0 201 290 1344 2382 Size: 201 89 1054 1038 Fragments arranged by size: 1054 1038 201 89 BsiHKAI G_wGCw'C Cuts at: 0 1006 1934 2087 2382 Size: 1006 928 153 295 Fragments arranged by size: 1006 928 295 153 BsmBI CGTCTCn'nnnn_ Cuts at: 0 677 1221 2382 Size: 677 544 1161 Fragments arranged by size: 1161 677 544 Bsp1286I G_dGCh'C Cuts at: 0 580 640 752 845 870 880 1006 1273 Size: 580 60 112 93 25 10 126 267 Cuts at: 1273 1463 1643 1766 1900 1934 2087 2185 2382 Size: 190 180 123 134 34 153 98 197 Fragments arranged by size: 580 267 197 190 180 153 134 126 123 112 98 93 60 34 25 10 BspEI T'CCGG_A Cuts at: 0 1635 2100 2382 Size: 1635 465 282 Fragments arranged by size: 1635 465 282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3 62 767 1430 2382 Size: 3 59 705 663 952 Fragments arranged by size: 952 705 663 59 3 BsrBI CCG'CTC Cuts at: 0 203 1870 2382 Size: 203 1667 512 Fragments arranged by size: 1667 512 203 BsrFI r'CCGG_y Cuts at: 0 522 1980 2251 2382 Size: 522 1458 271 131 Fragments arranged by size: 1458 522 271 131 BssHII G'CGCG_C Cuts at: 0 160 1661 2382 Size: 160 1501 721 Fragments arranged by size: 1501 721 160 BstAPI GCAn_nnn'nTGC Cuts at: 0 774 2144 2382 Size: 774 1370 238 Fragments arranged by size: 1370 774 238 BstXI CCAn_nnnn'nTGG Cuts at: 0 388 570 1524 2382 Size: 388 182 954 858 Fragments arranged by size: 954 858 388 182 BtgI C'CryG_G Cuts at: 0 105 482 837 1265 2111 2382 Size: 105 377 355 428 846 271 Fragments arranged by size: 846 428 377 355 271 105 BtsI GCAGTG_nn' Cuts at: 0 1517 1702 1940 2382 Size: 1517 185 238 442 Fragments arranged by size: 1517 442 238 185 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2188 2223 2382 Size: 2188 35 159 Fragments arranged by size: 2188 159 35 DrdI GACnn_nn'nnGTC Cuts at: 0 945 2382 Size: 945 1437 EaeI y'GGCC_r Cuts at: 0 198 287 788 911 1055 1304 1341 1865 Size: 198 89 501 123 144 249 37 524 Cuts at: 1865 2114 2382 Size: 249 268 Fragments arranged by size: 524 501 268 249 249 198 144 123 89 37 EagI C'GGCC_G Cuts at: 0 198 287 1341 2382 Size: 198 89 1054 1041 Fragments arranged by size: 1054 1041 198 89 EarI CTCTTCn'nnn_ Cuts at: 0 948 2382 Size: 948 1434 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 743 1728 1769 1775 2382 Size: 743 985 41 6 607 Fragments arranged by size: 985 743 607 41 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 451 635 1272 1393 1687 1744 2382 Size: 190 261 184 637 121 294 57 638 Fragments arranged by size: 638 637 294 261 190 184 121 57 EcoO109I rG'GnC_Cy Cuts at: 0 576 613 733 842 876 877 1050 1347 Size: 576 37 120 109 34 1 173 297 Cuts at: 1347 1568 1804 2025 2382 Size: 221 236 221 357 Fragments arranged by size: 576 357 297 236 221 221 173 120 109 37 34 1 EcoRI G'AATT_C Cuts at: 0 142 2382 Size: 142 2240 FspI TGC'GCA Cuts at: 0 1078 1618 2382 Size: 1078 540 764 Fragments arranged by size: 1078 764 540 HaeII r_GCGC'y Cuts at: 0 131 438 452 797 1410 1469 1723 1759 Size: 131 307 14 345 613 59 254 36 Cuts at: 1759 2004 2047 2063 2382 Size: 245 43 16 319 Fragments arranged by size: 613 345 319 307 254 245 131 59 43 36 16 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 840 872 1201 1233 2216 2248 Size: 187 32 621 32 329 32 983 32 Cuts at: 2248 2382 Size: 134 Fragments arranged by size: 983 621 329 187 134 32 32 32 32 HincII GTy'rAC Cuts at: 0 117 2382 Size: 117 2265 KasI G'GCGC_C Cuts at: 0 1406 1465 1755 2043 2382 Size: 1406 59 290 288 339 Fragments arranged by size: 1406 339 290 288 59 KpnI G_GTAC'C Cuts at: 0 899 2382 Size: 899 1483 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1838 2382 Size: 1838 544 MscI TGG'CCA Cuts at: 0 1306 2116 2382 Size: 1306 810 266 Fragments arranged by size: 1306 810 266 MslI CAynn'nnrTG Cuts at: 0 386 1879 2382 Size: 386 1493 503 Fragments arranged by size: 1493 503 386 MspA1I CmG'CkG Cuts at: 0 107 168 283 484 655 1502 2123 2273 Size: 107 61 115 201 171 847 621 150 Cuts at: 2273 2382 Size: 109 Fragments arranged by size: 847 621 201 171 150 115 109 107 61 NaeI GCC'GGC Cuts at: 0 1982 2382 Size: 1982 400 NarI GG'CG_CC Cuts at: 0 1407 1466 1756 2044 2382 Size: 1407 59 290 288 338 Fragments arranged by size: 1407 338 290 288 59 NcoI C'CATG_G Cuts at: 0 2111 2382 Size: 2111 271 NgoMIV G'CCGG_C Cuts at: 0 1980 2382 Size: 1980 402 NotI GC'GGCC_GC Cuts at: 0 287 2382 Size: 287 2095 NruI TCG'CGA Cuts at: 0 2335 2382 Size: 2335 47 NspI r_CATG'y Cuts at: 0 66 1476 2382 Size: 66 1410 906 Fragments arranged by size: 1410 906 66 PasI CC'CwG_GG Cuts at: 0 68 828 862 2241 2382 Size: 68 760 34 1379 141 Fragments arranged by size: 1379 760 141 68 34 PflMI CCAn_nnn'nTGG Cuts at: 0 80 828 2382 Size: 80 748 1554 Fragments arranged by size: 1554 748 80 PfoI T'CCnGG_A Cuts at: 0 25 191 2382 Size: 25 166 2191 Fragments arranged by size: 2191 166 25 PpuMI rG'GwC_Cy Cuts at: 0 613 1804 2025 2382 Size: 613 1191 221 357 Fragments arranged by size: 1191 613 357 221 PspOMI G'GGCC_C Cuts at: 0 576 841 876 2382 Size: 576 265 35 1506 Fragments arranged by size: 1506 576 265 35 PvuII CAG'CTG Cuts at: 0 168 1502 2382 Size: 168 1334 880 Fragments arranged by size: 1334 880 168 RsrII CG'GwC_CG Cuts at: 0 1852 2382 Size: 1852 530 SacII CC_GC'GG Cuts at: 0 108 485 2382 Size: 108 377 1897 Fragments arranged by size: 1897 377 108 SanDI GG'GwC_CC Cuts at: 0 613 1804 2382 Size: 613 1191 578 Fragments arranged by size: 1191 613 578 SfiI GGCCn_nnn'nGGCC Cuts at: 0 575 2245 2382 Size: 575 1670 137 Fragments arranged by size: 1670 575 137 SfoI GGC'GCC Cuts at: 0 1408 1467 1757 2045 2382 Size: 1408 59 290 288 337 Fragments arranged by size: 1408 337 290 288 59 SgrAI Cr'CCGG_yG Cuts at: 0 522 2251 2382 Size: 522 1729 131 Fragments arranged by size: 1729 522 131 SmaI CCC'GGG Cuts at: 0 210 719 902 1559 2069 2382 Size: 210 509 183 657 510 313 Fragments arranged by size: 657 510 509 313 210 183 SphI G_CATG'C Cuts at: 0 66 2382 Size: 66 2316 StuI AGG'CCT Cuts at: 0 1630 2382 Size: 1630 752 StyI C'CwwG_G Cuts at: 0 1913 2111 2308 2382 Size: 1913 198 197 74 Fragments arranged by size: 1913 198 197 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 958 2382 Size: 958 1424 TatI w'GTAC_w Cuts at: 0 1283 2382 Size: 1283 1099 Tth111I GACn'n_nGTC Cuts at: 0 1213 2382 Size: 1213 1169 XbaI T'CTAG_A Cuts at: 0 2221 2382 Size: 2221 161 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1227 2382 Size: 1227 1155 XmaI C'CCGG_G Cuts at: 0 208 717 900 1557 2067 2382 Size: 208 509 183 657 510 315 Fragments arranged by size: 657 510 509 315 208 183 XmnI GAAnn'nnTTC Cuts at: 0 1249 1336 2382 Size: 1249 87 1046 Fragments arranged by size: 1249 1046 87 Enzymes that do cut: AarI Acc65I AclI AcuI AfeI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI BtsI CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI PasI PflMI PfoI PpuMI PspOMI PvuII RsrII SacII SanDI SfiI SfoI SgrAI SmaI SphI StuI StyI TaqII TatI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AflII AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII BmtI BpuEI BsaBI BsiWI BsmI BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI NdeI NheI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SmlI SnaBI SpeI SrfI SspI SwaI TstI XhoI ZraI