################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwI 548 GGATC GGATC(4/5) AlwNI 206 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 109 GGGCCC GGGCC^C AspHI 35 GAGCAC G[AT]GC[AT]^C AvaI 67 CTCGAG C^[CT]CG[GA]G AvaII 317 GGTCC G^G[AT]CC BanI 244 GGCACC G^G[CT][GA]CC BbsI 790 GAAGAC GAAGAC(2/6) BcnI 300 CCGGG CC^[GC]GG BfaI 655 CTAG C^TAG BglI 111 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 413 AGATCT A^GATCT BpuAI 790 GAAGAC GAAGAC(2/6) BsaAI 196 CACGTA [CT]AC^GT[GA] BsiHKAI 35 GAGCAC G[AT]GC[AT]^C BsmAI 603 GAGAC GTCTC(1/5) BsmI 516 GCATTC GAATGC(1/-1) BsoBI 67 CTCGAG C^[CT]CG[GA]G Bsp1407I 431 TGTACA T^GTACA BspHI 797 TCATGA T^CATGA BsrDI 138 CATTGC GCAATG(2/0) BsrGI 431 TGTACA T^GTACA BstF5I 750 GGATG GGATG(2/0) BstYI 413 AGATCT [GA]^GATC[CT] CfoI 105 GCGC GCG^C DraII 109 GGGCCCC [GA]G^G[ACGT]CC[CT] EarI 600 GAAGAG CTCTTC(1/4) EcoO109I 109 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 50 CCATGG C^C[AT][AT]GG FokI 750 GGATG GGATG(9/13) HhaI 105 GCGC GCG^C HinP1I 105 GCGC G^CGC Ksp632I 600 GAAGAG CTCTTC(1/4) KspI 5 CCGCGG CCGC^GG MaeI 655 CTAG C^TAG MflI 413 AGATCT [GA]^GATC[CT] NciI 300 CCGGG CC^[GC]GG NcoI 50 CCATGG C^CATGG PaeR7I 67 CTCGAG C^TCGAG RcaI 797 TCATGA T^CATGA SacII 5 CCGCGG CCGC^GG SmlI 67 CTCGAG C^T[CT][GA]AG SspBI 431 TGTACA T^GTACA StuI 90 AGGCCT AGG^CCT StyI 50 CCATGG C^C[AT][AT]GG Tsp509I 639 AATT ^AATT XbaI 654 TCTAGA T^CTAGA XhoI 67 CTCGAG C^TCGAG XhoII 413 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BalI 53 TGGCCA TGG^CCA 669 TGGCCA BsgI 94 CTGCAC GTGCAG(16/14) 258 CTGCAC DpnII 414 GATC ^GATC 549 GATC DsaI 5 CCGCGG C^C[GA][CT]GG 50 CCATGG HapII 300 CCGG C^CGG 546 CCGG HinfI 459 GAATC G^A[ACGT]TC 825 GAATC HpaII 300 CCGG C^CGG 546 CCGG MaeII 157 ACGT A^CGT 197 ACGT MboI 414 GATC ^GATC 549 GATC MboII 600 GAAGA GAAGA(8/7) 790 GAAGA MluNI 53 TGGCCA TGG^CCA 669 TGGCCA MscI 53 TGGCCA TGG^CCA 669 TGGCCA MspI 300 CCGG C^CGG 546 CCGG NdeII 414 GATC ^GATC 549 GATC PvuII 364 CAGCTG CAG^CTG 661 CAGCTG Sau3AI 414 GATC ^GATC 549 GATC SfcI 203 CTACAG C^T[GA][CT]AG 212 CTGTAG TfiI 459 GAATC G^A[AT]TC 825 GAATC Tsp45I 437 GTGAC ^GT[GC]AC 485 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccII AciI AfaI AluI BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstUI BstXI Cac8I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeIII ItaI MaeIII MnlI MslI MspA1I MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI TaqI TseI TspRI TthHB8I