(Circular) (Six-base) MAPSORT of: pFN21AE3107.seq Check: 785 from: 1 to: 5536 pFN21AE3107 5536 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 3599 4983 5251 1632 Size: 400 1567 1384 268 1917 Fragments arranged by size: 1917 1567 1384 400 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 2165 1166 Size: 999 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 2216 3279 4851 2216 Size: 1063 1572 2901 Fragments arranged by size: 2901 1572 1063 ApaI G_GGCC'C Cuts at: 2118 2118 Size: 5536 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2845 3071 3223 5332 1493 Size: 1352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2861 1870 Size: 73 129 789 4545 Fragments arranged by size: 4545 789 129 73 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2857 4312 618 Size: 325 217 627 462 608 1455 1842 Fragments arranged by size: 1842 1455 627 608 462 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2118 2379 2855 1391 Size: 678 49 261 476 4072 Fragments arranged by size: 4072 678 476 261 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 928 Size: 971 903 3662 Fragments arranged by size: 3662 971 903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 4147 136 Size: 107 122 71 631 605 450 2025 1525 Fragments arranged by size: 2025 1525 631 605 450 122 107 71 BglII A'GATC_T Cuts at: 2418 5531 2418 Size: 3113 2423 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2118 3597 4753 1790 Size: 328 1479 1156 2573 Fragments arranged by size: 2573 1479 1156 328 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2074 2393 2564 4196 1239 Size: 835 319 171 1632 2579 Fragments arranged by size: 2579 1632 835 319 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3661 4526 4824 3661 Size: 865 298 4373 Fragments arranged by size: 4373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2203 3327 493 Size: 711 999 1124 2702 Fragments arranged by size: 2702 1124 999 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 2645 2648 1186 Size: 891 568 3 4074 Fragments arranged by size: 4074 891 568 3 BseYI C'CCAG_C Cuts at: 4739 5235 4739 Size: 496 5040 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2248 1163 Size: 573 348 164 4451 Fragments arranged by size: 4451 573 348 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2044 3597 3682 4753 2044 Size: 1553 85 1071 2827 Fragments arranged by size: 2827 1553 1071 85 BsmI GAATG_Cn' Cuts at: 2521 3047 3140 2521 Size: 526 93 4917 Fragments arranged by size: 4917 526 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2069 2118 2379 2855 3597 3682 Size: 399 254 25 49 261 476 742 85 Cuts at: 3682 4753 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 261 254 85 49 25 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 2802 3425 5155 2802 Size: 623 1730 3183 Fragments arranged by size: 3183 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 844 Size: 2047 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3423 1394 Size: 429 1600 3507 Fragments arranged by size: 3507 1600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 4031 4205 65 Size: 1183 894 1889 174 1396 Fragments arranged by size: 1889 1396 1183 894 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4180 4367 1700 Size: 110 2370 187 2869 Fragments arranged by size: 2869 2370 187 110 BsrGI T'GTAC_A Cuts at: 96 2436 96 Size: 2340 3196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstXI CCAn_nnnn'nTGG Cuts at: 2246 2279 2319 2246 Size: 33 40 5463 Fragments arranged by size: 5463 40 33 BstYI r'GATC_y Cuts at: 1972 2418 2866 3233 3618 3635 5087 5173 5185 Size: 446 448 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 448 446 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2055 513 Size: 552 939 6 45 3994 Fragments arranged by size: 3994 939 552 45 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2265 3052 3846 3866 2265 Size: 787 794 20 3935 Fragments arranged by size: 3935 794 787 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2058 2674 Size: 54 1425 50 275 18 177 51 616 Cuts at: 2674 2983 3872 5501 8 Size: 309 889 1629 43 Fragments arranged by size: 1629 1425 889 616 309 275 177 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 3466 1353 Size: 1247 866 3423 Fragments arranged by size: 3423 1247 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4123 4497 4643 387 Size: 1064 138 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2074 2393 2564 3599 4196 4983 Size: 393 400 42 319 171 1035 597 787 Cuts at: 4983 5251 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 787 597 400 393 319 268 171 42 EcoO109I rG'GnC_Cy Cuts at: 2115 2115 Size: 5536 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2708 2734 2708 Size: 26 5510 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 4683 1678 Size: 3005 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373 Size: 32 135 32 2605 32 42 32 2626 Fragments arranged by size: 2626 2605 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 4650 4834 1305 Size: 1428 1917 184 2007 Fragments arranged by size: 2007 1917 1428 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 2676 10 Size: 54 1425 343 228 616 2870 Fragments arranged by size: 2870 1425 616 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2165 2741 3494 3853 4012 518 Size: 648 981 18 576 753 359 159 2042 Fragments arranged by size: 2042 981 753 648 576 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2012 2371 2668 2952 3629 4777 5022 1606 Size: 406 359 297 284 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 406 359 297 284 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2055 513 Size: 552 939 51 3994 Fragments arranged by size: 3994 939 552 51 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2894 4439 1132 Size: 97 1665 1545 2229 Fragments arranged by size: 2229 1665 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PspOMI G'GGCC_C Cuts at: 2114 2114 Size: 5536 PspXI vC'TCGA_Gb Cuts at: 2072 2072 Size: 5536 PstI C_TGCA'G Cuts at: 830 2888 830 Size: 2058 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 PvuII CAG'CTG Cuts at: 2371 2668 2371 Size: 297 5239 SacII CC_GC'GG Cuts at: 2013 2013 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2208 2217 2884 4019 4700 4891 826 Size: 219 1163 9 667 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 681 667 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2863 1872 Size: 991 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 3640 4541 4803 820 Size: 197 926 129 1568 901 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 2097 1517 Size: 580 4956 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 2975 513 Size: 552 939 51 920 3074 Fragments arranged by size: 3074 939 920 552 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 3563 3580 3733 3918 4363 325 Size: 1255 687 1296 17 153 185 445 1498 Fragments arranged by size: 1498 1296 1255 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2365 2413 2436 Size: 274 80 33 51 1443 388 48 23 Cuts at: 2436 3782 5494 96 Size: 1346 1712 138 Fragments arranged by size: 1712 1443 1346 388 274 138 80 51 48 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5496 5528 5496 Size: 32 5504 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2659 2872 2659 Size: 213 5323 XhoI C'TCGA_G Cuts at: 1943 2072 1943 Size: 129 5407 XmaI C'CCGG_G Cuts at: 1870 2861 1870 Size: 991 4545 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI