Restriction map for
pFN21AB8349
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI Ksp632I MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AatII 565 GACGTC GACGT^C
Acc65I 143 GGTACC G^GTACC
AccI 304 GTCTAC GT^[AC][GT]AC
AccII 30 CGCG CG^CG
AcsI 727 GAATTC [GA]^AATT[CT]
AlwNI 733 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 116 GGGCCC GGGCC^C
ApoI 727 GAATTC [GA]^AATT[CT]
Asp700I 630 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 143 GGTACC G^GTACC
BalI 56 TGGCCA TGG^CCA
BamHI 164 GGATCC G^GATCC
BanII 116 GGGCCC G[GA]GC[CT]^C
BbeI 383 GGCGCC GGCGC^C
BbrPI 10 CACGTG CAC^GTG
BbsI 741 GAAGAC GAAGAC(2/6)
BglI 423 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 772 CTCCAG CTGGAG(16/14)
BpuAI 741 GAAGAC GAAGAC(2/6)
BsaAI 10 CACGTG [CT]AC^GT[GA]
BseRI 293 GAGGAG GAGGAG(10/8)
BsgI 7 CTGCAC GTGCAG(16/14)
BsiEI 155 CGGCCG CG[GA][CT]^CG
BstUI 30 CGCG CG^CG
BstYI 164 GGATCC [GA]^GATC[CT]
CfoI 384 GCGC GCG^C
DraII 482 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 155 CGGCCG C^GGCCG
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
EcoO109I 482 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 727 GAATTC G^AATTC
HaeII 383 GGCGCC [GA]GCGC^[CT]
HhaI 384 GCGC GCG^C
HinP1I 384 GCGC G^CGC
KasI 383 GGCGCC G^GCGCC
KpnI 143 GGTACC GGTAC^C
KspI 29 CCGCGG CCGC^GG
MflI 164 GGATCC [GA]^GATC[CT]
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MslI 433 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 30 CGCG CG^CG
NarI 383 GGCGCC GG^CGCC
NcoI 345 CCATGG C^CATGG
NspI 432 GCATGT [GA]CATG^[CT]
PaeR7I 655 CTCGAG C^TCGAG
PflMI 107 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 10 CACGTG CAC^GTG
PmlI 10 CACGTG CAC^GTG
SacII 29 CCGCGG CCGC^GG
SfoI 383 GGCGCC GGC^GCC
SmaI 139 CCCGGG CCC^GGG
SmlI 655 CTCGAG C^T[CT][GA]AG
TaqI 656 TCGA T^CGA
TfiI 377 GAATC G^A[AT]TC
Tsp509I 728 AATT ^AATT
TthHB8I 656 TCGA T^CGA
Van91I 107 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 655 CTCGAG C^TCGAG
XhoII 164 GGATCC [GA]^GATC[CT]
XmaCI 139 CCCGGG C^CCGGG
XmaI 139 CCCGGG C^CCGGG
XmnI 630 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 144 GTAC GT^AC
652 GTAC
AlwI 165 GATCC GGATC(4/5)
164 GGATC
AvaI 139 CCCGGG C^[CT]CG[GA]G
655 CTCGAG
BsoBI 139 CCCGGG C^[CT]CG[GA]G
655 CTCGAG
BsrBI 40 CCGCTC CCGCTC(-3/-3)
658 GAGCGG
BsrI 353 ACTGG ACTGG(1/-1)
107 CCAGT
BstF5I 417 CATCC GGATG(2/0)
317 GGATG
DdeI 395 CTGAG C^T[ACGT]AG
746 CTCAG
DpnII 165 GATC ^GATC
624 GATC
DsaI 29 CCGCGG C^C[GA][CT]GG
345 CCATGG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
345 CCATGG
FokI 417 CATCC GGATG(9/13)
317 GGATG
HgaI 65 GCGTC GACGC(5/10)
302 GCGTC
MboI 165 GATC ^GATC
624 GATC
NdeII 165 GATC ^GATC
624 GATC
PleI 491 GACTC GAGTC(4/5)
744 GACTC
PvuII 93 CAGCTG CAG^CTG
218 CAGCTG
RsaI 144 GTAC GT^AC
652 GTAC
Sau3AI 165 GATC ^GATC
624 GATC
StyI 239 CCAAGG C^C[AT][AT]GG
345 CCATGG
XcmI 520 CCACCCCACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
768 CCACCTCCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I EaeI EcoRII
FauI Fnu4HI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI MaeII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PstI
Sau96I ScrFI SfaNI SfcI TseI
TspRI