Restriction map for
pFN21AE3339
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AscI AseI AsnI Asp700I AviII
AvrII BbeI BciVI BfrI BglII
BlnI BseAI BsiEI BsiWI BspDI
BspEI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI MluI MroI NarI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 2107 GAATTT [GA]^AATT[CT]
AflIII 191 ACATGT A^C[GA][CT]GT
AgeI 2528 ACCGGT A^CCGGT
AhdI 472 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2107 GAATTT [GA]^AATT[CT]
AspEI 472 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 595 GTCTTC GAAGAC(2/6)
BbvCI 2848 GCTGAGG CCTCAGC(-5/-2)
BcgI 1815 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2457 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2457 GCTGAGC GC^T[ACGT]AGC
BpuAI 595 GTCTTC GAAGAC(2/6)
BsmBI 2751 CGTCTC CGTCTC(1/5)
BspLU11I 191 ACATGT A^CATGT
BstEII 432 GGTAACC G^GT[ACGT]ACC
BstPI 432 GGTAACC G^GT[ACGT]ACC
CelII 2457 GCTGAGC GC^T[ACGT]AGC
DrdI 2155 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 472 GACCTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 432 GGTAACC G^GT[ACGT]ACC
HindIII 2623 AAGCTT A^AGCTT
KspI 261 CCGCGG CCGC^GG
MfeI 249 CAATTG C^AATTG
MunI 249 CAATTG C^AATTG
NaeI 2606 GCCGGC GCC^GGC
NcoI 2822 CCATGG C^CATGG
NdeI 2591 CATATG CA^TATG
NgoMIV 2606 GCCGGC G^CCGGC
PaeR7I 2764 CTCGAG C^TCGAG
PflFI 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2528 ACCGGT A^CCGGT
PvuII 2158 CAGCTG CAG^CTG
SacII 261 CCGCGG CCGC^GG
SapI 2508 GAAGAGC GCTCTTC(1/4)
SmlI 2764 CTCGAG C^T[CT][GA]AG
StuI 1347 AGGCCT AGG^CCT
Tth111I 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1488 TCTAGA T^CTAGA
XhoI 2764 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1475 GGTACC G^GTACC
2525 GGTACC
AcyI 1079 GACGCC G[GA]^CG[CT]C
2068 GGCGTC
Alw44I 728 GTGCAC G^TGCAC
1168 GTGCAC
AlwNI 166 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
2378 CAGCCCCTG
Aor51HI 506 AGCGCT AGC^GCT
605 AGCGCT
ApaLI 728 GTGCAC G^TGCAC
1168 GTGCAC
Asp718I 1475 GGTACC G^GTACC
2525 GGTACC
BamHI 132 GGATCC G^GATCC
2735 GGATCC
BclI 1587 TGATCA T^GATCA
2096 TGATCA
BsaHI 1079 GACGCC G[GA]^CG[CT]C
2068 GGCGTC
BseRI 268 GAGGAG GAGGAG(10/8)
1634 GAGGAG
Bsp1407I 188 TGTACA T^GTACA
374 TGTACA
BspMI 688 ACCTGC ACCTGC(4/8)
1203 ACCTGC
BsrDI 2391 CATTGC GCAATG(2/0)
2676 CATTGC
BsrFI 2528 ACCGGT [GA]^CCGG[CT]
2606 GCCGGC
BsrGI 188 TGTACA T^GTACA
374 TGTACA
BssHII 67 GCGCGC G^CGCGC
69 GCGCGC
Cfr10I 2528 ACCGGT [GA]^CCGG[CT]
2606 GCCGGC
EarI 2702 CTCTTC CTCTTC(1/4)
2508 GAAGAG
Eco47III 506 AGCGCT AGC^GCT
605 AGCGCT
EcoNI 2010 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2135 CCTTCCTGAGG
FbaI 1587 TGATCA T^GATCA
2096 TGATCA
Hin1I 1079 GACGCC G[GA]^CG[CT]C
2068 GGCGTC
KpnI 1475 GGTACC GGTAC^C
2525 GGTACC
Ksp632I 2702 CTCTTC CTCTTC(1/4)
2508 GAAGAG
PflMI 139 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCAGGATGTGG
SacI 956 GAGCTC GAGCT^C
2569 GAGCTC
SspBI 188 TGTACA T^GTACA
374 TGTACA
Van91I 139 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaBI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI