(Circular) (Six-base) MAPSORT of: pFN21AE3339.seq Check: 1572 from: 1 to: 7633 pFN21AE3339 7633 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3217 3217 Size: 7633 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5443 278 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4976 1937 Size: 3039 4594 Acc65I G'GTAC_C Cuts at: 3480 4530 4954 3480 Size: 1050 424 6159 Fragments arranged by size: 6159 1050 424 AclI AA'CG_TT Cuts at: 1563 5760 6133 1563 Size: 4197 373 3063 Fragments arranged by size: 4197 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 4410 4616 5696 7080 7348 1632 Size: 2180 598 206 1080 1384 268 1917 Fragments arranged by size: 2180 1917 1384 1080 598 268 206 AfeI AGC'GCT Cuts at: 2513 2612 2513 Size: 99 7534 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7436 AflIII A'CryG_T Cuts at: 1203 2196 6532 7509 1203 Size: 993 4336 977 1327 Fragments arranged by size: 4336 1327 993 977 AgeI A'CCGG_T Cuts at: 4533 6464 4533 Size: 1931 5702 AhdI GACnn_n'nnGTC Cuts at: 2482 6362 2482 Size: 3880 3753 AleI CACnn'nnGTG Cuts at: 1166 3764 1166 Size: 2598 5035 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2925 4671 1184 Size: 1741 1746 4146 Fragments arranged by size: 4146 1746 1741 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2288 2320 2288 Size: 32 7601 AlwNI CAG_nnn'CTG Cuts at: 2176 4388 5376 6948 2176 Size: 2212 988 1572 2861 Fragments arranged by size: 2861 2212 1572 988 ApaI G_GGCC'C Cuts at: 2978 3150 3193 2978 Size: 172 43 7418 Fragments arranged by size: 7418 172 43 ApaLI G'TGCA_C Cuts at: 2733 3173 5690 6846 2733 Size: 440 2517 1156 3520 Fragments arranged by size: 3520 2517 1156 440 ApoI r'AATT_y Cuts at: 1493 4112 4942 5168 5320 7429 1493 Size: 2619 830 226 152 2109 1697 Fragments arranged by size: 2619 2109 1697 830 226 152 AseI AT'TA_AT Cuts at: 160 1032 6187 160 Size: 872 5155 1606 Fragments arranged by size: 5155 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2566 2966 3192 3852 4769 4886 Size: 73 122 501 400 226 660 917 117 Cuts at: 4886 4958 1870 Size: 72 4545 Fragments arranged by size: 4545 917 660 501 400 226 122 117 73 72 BamHI G'GATC_C Cuts at: 2137 4740 4963 5330 2137 Size: 2603 223 367 4440 Fragments arranged by size: 4440 2603 367 223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2570 2773 2787 3480 4530 Size: 325 217 627 783 203 14 693 1050 Cuts at: 4530 4890 4954 6409 618 Size: 360 64 1455 1842 Fragments arranged by size: 1842 1455 1050 783 693 627 360 325 217 203 64 14 BanII G_rGCy'C Cuts at: 1391 2965 2978 3150 3193 3201 3724 4132 4578 Size: 1574 13 172 43 8 523 408 446 Cuts at: 4578 4952 1391 Size: 374 4072 Fragments arranged by size: 4072 1574 523 446 408 374 172 43 13 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2593 928 Size: 971 694 5968 Fragments arranged by size: 5968 971 694 BbvCI CC'TCA_GC Cuts at: 4854 4854 Size: 7633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3809 3843 5824 5858 1903 Size: 34 1872 34 1981 34 3678 Fragments arranged by size: 3678 1981 1872 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5525 6735 5525 Size: 1210 6423 BclI T'GATC_A Cuts at: 1548 3592 4101 1548 Size: 2044 509 5080 Fragments arranged by size: 5080 2044 509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 2579 2760 Size: 107 122 71 631 605 354 553 181 Cuts at: 2760 2850 4728 6244 136 Size: 90 1878 1516 1525 Fragments arranged by size: 1878 1525 1516 631 605 553 354 181 122 107 90 71 BglII A'GATC_T Cuts at: 7628 7628 Size: 7633 BlpI GC'TnA_GC Cuts at: 4463 5050 4463 Size: 587 7046 Bme1580I G_kGCm'C Cuts at: 1790 2737 2897 2978 3131 3150 3177 3193 4609 Size: 947 160 81 153 19 27 16 1416 Cuts at: 4609 4893 5694 6850 1790 Size: 284 801 1156 2573 Fragments arranged by size: 2573 1416 1156 947 801 284 160 153 81 27 19 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7633 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3112 3578 5364 Size: 416 53 83 399 24 1695 466 1786 Cuts at: 5364 6322 442 Size: 958 1753 Fragments arranged by size: 1786 1753 1695 958 466 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7633 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2533 2533 Size: 7633 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3657 3794 4664 4862 6293 1239 Size: 2418 137 870 198 1431 2579 Fragments arranged by size: 2579 2418 1431 870 198 137 Bpu10I CC'TnA_GC Cuts at: 3020 4854 3020 Size: 1834 5799 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5758 6623 6921 5758 Size: 865 298 6470 Fragments arranged by size: 6470 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 2469 2615 6296 882 Size: 617 858 112 146 3681 2219 Fragments arranged by size: 3681 2219 858 617 146 112 BsaAI yAC'GTr Cuts at: 493 1204 2293 2892 3457 5424 493 Size: 711 1089 599 565 1967 2702 Fragments arranged by size: 2702 1967 1089 711 599 565 BsaBI GATnn'nnATC Cuts at: 3768 4100 4370 4874 7627 3768 Size: 332 270 504 2753 3774 Fragments arranged by size: 3774 2753 504 332 270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3085 4074 5440 5822 Size: 53 83 186 936 1552 989 1366 382 Cuts at: 5822 6457 275 Size: 635 1451 Fragments arranged by size: 1552 1451 1366 989 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2859 3708 4533 6066 6464 6738 6885 7296 2859 Size: 849 825 1533 398 274 147 411 3196 Fragments arranged by size: 3196 1533 849 825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2288 2318 3633 3663 4652 Size: 30 165 30 885 30 1315 30 989 Cuts at: 4652 4682 1178 Size: 30 4129 Fragments arranged by size: 4129 1315 989 885 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 3654 1186 Size: 1102 1366 5165 Fragments arranged by size: 5165 1366 1102 BseYI C'CCAG_C Cuts at: 2122 2669 2898 3067 3286 3723 3941 4590 4718 Size: 547 229 169 219 437 218 649 128 Cuts at: 4718 4814 6836 7332 2122 Size: 96 2022 496 2423 Fragments arranged by size: 2423 2022 649 547 496 437 229 219 218 169 128 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3437 3699 3813 4784 1163 Size: 573 1701 262 114 971 4012 Fragments arranged by size: 4012 1701 971 573 262 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5083 5844 6872 1540 Size: 275 186 3082 761 1028 2301 Fragments arranged by size: 3082 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2737 2909 2965 3177 4578 5694 5779 6850 2737 Size: 172 56 212 1401 1116 85 1071 3520 Fragments arranged by size: 3520 1401 1116 1071 212 172 85 56 BsmI GAATG_Cn' Cuts at: 2406 3561 4902 5144 5237 2406 Size: 1155 1341 242 93 4802 Fragments arranged by size: 4802 1341 1155 242 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4762 4762 Size: 7633 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2737 2897 2909 2965 2978 3131 3150 Size: 399 947 160 12 56 13 153 19 Cuts at: 3150 3177 3193 3201 3724 4132 4578 4609 4893 Size: 27 16 8 523 408 446 31 284 Cuts at: 4893 4952 5694 5779 6850 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 947 742 523 446 408 399 284 160 153 85 59 56 31 27 19 16 13 12 8 BspEI T'CCGG_A Cuts at: 7296 7296 Size: 7633 BspHI T'CATG_A Cuts at: 3595 4098 4578 5522 7252 3595 Size: 503 480 944 1730 3976 Fragments arranged by size: 3976 1730 944 503 480 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 3217 4988 844 Size: 1858 515 1771 3489 Fragments arranged by size: 3489 1858 1771 515 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2949 4153 5520 1394 Size: 429 188 938 1204 1367 3507 Fragments arranged by size: 3507 1367 1204 938 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 4395 4680 6128 6302 65 Size: 1183 3147 285 1448 174 1396 Fragments arranged by size: 3147 1448 1396 1183 285 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4533 4611 6277 6464 1700 Size: 110 2723 78 1666 187 2869 Fragments arranged by size: 2869 2723 1666 187 110 78 BsrGI T'GTAC_A Cuts at: 96 2193 2379 96 Size: 2097 186 5350 Fragments arranged by size: 5350 2097 186 BssHII G'CGCG_C Cuts at: 1925 2072 2074 1925 Size: 147 2 7484 Fragments arranged by size: 7484 147 2 BssSI C'ACGA_G Cuts at: 5693 6705 5693 Size: 1012 6621 BstAPI GCAn_nnn'nTGC Cuts at: 794 2748 2837 4060 4908 794 Size: 1954 89 1223 848 3519 Fragments arranged by size: 3519 1954 1223 848 89 BstBI TT'CG_AA Cuts at: 6437 6437 Size: 7633 BstEII G'GTnAC_C Cuts at: 1184 2437 1184 Size: 1253 6380 BstXI CCAn_nnnn'nTGG Cuts at: 3161 3766 4814 3161 Size: 605 1048 5980 Fragments arranged by size: 5980 1048 605 BstYI r'GATC_y Cuts at: 1972 2137 2259 4365 4407 4740 4963 5330 5715 Size: 165 122 2106 42 333 223 367 385 Cuts at: 5715 5732 7184 7270 7282 7628 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2106 1977 1452 385 367 346 333 223 165 122 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2728 3034 3932 4145 2728 Size: 306 898 213 6216 Fragments arranged by size: 6216 898 306 213 BtgI C'CryG_G Cuts at: 513 1065 2004 2266 2442 2539 4827 513 Size: 552 939 262 176 97 2288 3319 Fragments arranged by size: 3319 2288 939 552 262 176 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4440 487 Size: 683 56 3214 3680 Fragments arranged by size: 3680 3214 683 56 BtsI GCAGTG_nn' Cuts at: 2746 2827 2907 3219 4880 5149 5943 5963 2746 Size: 81 80 312 1661 269 794 20 4416 Fragments arranged by size: 4416 1661 794 312 269 81 80 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7598 DraI TTT'AAA Cuts at: 4937 4937 Size: 7633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4166 6640 809 Size: 507 2850 2474 1802 Fragments arranged by size: 2850 2474 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2950 3298 3963 Size: 54 1425 50 275 18 1120 348 665 Cuts at: 3963 3997 4185 4613 5080 5969 7598 8 Size: 34 188 428 467 889 1629 43 Fragments arranged by size: 1629 1425 1120 889 665 467 428 348 275 188 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 5080 1537 Size: 275 3268 4090 Fragments arranged by size: 4090 3268 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4508 4713 5563 1353 Size: 3155 205 850 3423 Fragments arranged by size: 3423 3155 850 205 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6220 6594 6740 387 Size: 1064 138 4631 374 146 1280 Fragments arranged by size: 4631 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2963 4576 2963 Size: 1613 6020 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3657 3794 3812 4410 4616 4664 4862 Size: 393 2025 137 18 598 206 48 198 Cuts at: 4862 5696 6293 7080 7348 1239 Size: 834 597 787 268 1524 Fragments arranged by size: 2025 1524 834 787 598 597 393 268 206 198 137 48 18 EcoNI CCTnn'n_nnAGG Cuts at: 4019 4144 4019 Size: 125 7508 EcoO109I rG'GnC_Cy Cuts at: 2691 2724 2974 2975 2996 3146 3147 3189 3411 Size: 33 250 1 21 150 1 42 222 Cuts at: 3411 3572 3628 3823 3927 4249 4858 2691 Size: 161 56 195 104 322 609 5466 Fragments arranged by size: 5466 609 322 250 222 195 161 150 104 56 42 33 21 1 1 EcoRI G'AATT_C Cuts at: 4942 5320 4942 Size: 378 7255 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 7633 FspI TGC'GCA Cuts at: 6139 6139 Size: 7633 HaeII r_GCGC'y Cuts at: 1678 2017 2515 2614 6780 1678 Size: 339 498 99 4166 2531 Fragments arranged by size: 4166 2531 498 339 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 3332 3364 4350 Size: 32 135 32 896 32 832 32 986 Cuts at: 4350 4382 4483 4515 4732 4764 6274 6306 6348 Size: 32 101 32 217 32 1510 32 42 Cuts at: 6348 6380 1373 Size: 32 2626 Fragments arranged by size: 2626 1510 986 896 832 217 135 101 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4977 5223 669 Size: 988 281 3039 246 3079 Fragments arranged by size: 3079 3039 988 281 246 HindIII A'AGCT_T Cuts at: 748 4628 748 Size: 3880 3753 HpaI GTT'AAC Cuts at: 5223 5223 Size: 7633 KpnI G_GTAC'C Cuts at: 3484 4534 4958 3484 Size: 1050 424 6159 Fragments arranged by size: 6159 1050 424 MfeI C'AATT_G Cuts at: 2254 5232 2254 Size: 2978 4655 MluI A'CGCG_T Cuts at: 7509 7509 Size: 7633 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2440 4358 6747 6931 1305 Size: 1135 1918 2389 184 2007 Fragments arranged by size: 2389 2007 1918 1135 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3300 3965 3999 4187 10 Size: 54 1425 343 1468 665 34 188 3456 Fragments arranged by size: 3456 1468 1425 665 343 188 54 34 MslI CAynn'nnrTG Cuts at: 518 1166 3222 3326 3764 4236 4271 4781 5591 Size: 648 2056 104 438 472 35 510 810 Cuts at: 5591 5950 6109 518 Size: 359 159 2042 Fragments arranged by size: 2056 2042 810 648 510 472 438 359 159 104 35 MspA1I CmG'CkG Cuts at: 1606 2059 2268 2669 3727 4165 4853 5049 5726 Size: 453 209 401 1058 438 688 196 677 Cuts at: 5726 6874 7119 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1058 688 677 453 438 401 245 209 196 NaeI GCC'GGC Cuts at: 4613 4613 Size: 7633 NcoI C'CATG_G Cuts at: 513 1065 2004 4827 513 Size: 552 939 2823 3319 Fragments arranged by size: 3319 2823 939 552 NdeI CA'TA_TG Cuts at: 387 4597 387 Size: 4210 3423 NgoMIV G'CCGG_C Cuts at: 4611 4611 Size: 7633 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7633 NotI GC'GGCC_GC Cuts at: 5080 5080 Size: 7633 NspI r_CATG'y Cuts at: 1132 1229 2200 2746 2835 2991 3221 3338 4991 Size: 97 971 546 89 156 230 117 1653 Cuts at: 4991 6536 1132 Size: 1545 2229 Fragments arranged by size: 2229 1653 1545 971 546 230 156 117 97 89 PasI CC'CwG_GG Cuts at: 1348 2023 2333 3428 3501 4172 4354 4698 1348 Size: 675 310 1095 73 671 182 344 4283 Fragments arranged by size: 4283 1095 675 671 344 310 182 73 PciI A'CATG_T Cuts at: 2196 6532 2196 Size: 4336 3297 PflMI CCAn_nnn'nTGG Cuts at: 1108 2150 2401 1108 Size: 1042 251 6340 Fragments arranged by size: 6340 1042 251 PfoI T'CCnGG_A Cuts at: 3713 4323 3713 Size: 610 7023 PmeI GTTT'AAAC Cuts at: 4937 4937 Size: 7633 PmlI CAC'GTG Cuts at: 2293 2892 3457 2293 Size: 599 565 6469 Fragments arranged by size: 6469 599 565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2288 2320 5702 5734 7215 7247 2288 Size: 32 3382 32 1481 32 2674 Fragments arranged by size: 3382 2674 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2691 2996 3411 3572 3823 3927 4249 4858 2691 Size: 305 415 161 251 104 322 609 5466 Fragments arranged by size: 5466 609 415 322 305 251 161 104 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7633 PsiI TTA'TAA Cuts at: 5203 5203 Size: 7633 PspOMI G'GGCC_C Cuts at: 2974 3146 3189 2974 Size: 172 43 7418 Fragments arranged by size: 7418 172 43 PspXI vC'TCGA_Gb Cuts at: 4769 4769 Size: 7633 PstI C_TGCA'G Cuts at: 830 2304 2455 2753 3214 3525 4197 4985 830 Size: 1474 151 298 461 311 672 788 3478 Fragments arranged by size: 3478 1474 788 672 461 311 298 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7633 PvuII CAG'CTG Cuts at: 4165 4165 Size: 7633 SacI G_AGCT'C Cuts at: 2965 4578 2965 Size: 1613 6020 SacII CC_GC'GG Cuts at: 2269 2269 Size: 7633 SalI G'TCGA_C Cuts at: 1936 4975 1936 Size: 3039 4594 SanDI GG'GwC_CC Cuts at: 3411 3927 3411 Size: 516 7117 SapI GCTCTTCn'nnn_ Cuts at: 4508 4508 Size: 7633 SbfI CC_TGCA'GG Cuts at: 4985 4985 Size: 7633 ScaI AGT'ACT Cuts at: 5881 7593 5881 Size: 1712 5921 SfcI C'TryA_G Cuts at: 826 1045 2300 2451 2749 2778 3210 3521 4193 Size: 219 1255 151 298 29 432 311 672 Cuts at: 4193 4981 6116 6797 6988 826 Size: 788 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 788 681 672 432 311 298 219 191 151 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7633 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7633 SmaI CCC'GGG Cuts at: 1872 2067 2568 2968 3194 3854 4888 4960 1872 Size: 195 501 400 226 660 1034 72 4545 Fragments arranged by size: 4545 1034 660 501 400 226 195 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 4769 5737 6638 6900 820 Size: 197 926 2826 968 901 262 1553 Fragments arranged by size: 2826 1553 968 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5424 493 Size: 4931 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7633 SphI G_CATG'C Cuts at: 1132 2746 2991 3338 4991 1132 Size: 1614 245 347 1653 3774 Fragments arranged by size: 3774 1653 1614 347 245 SrfI GCCC'GGGC Cuts at: 4888 4888 Size: 7633 SspI AAT'ATT Cuts at: 5 52 5557 5 Size: 47 5505 2081 Fragments arranged by size: 5505 2081 47 StuI AGG'CCT Cuts at: 1517 3354 1517 Size: 1837 5796 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 3840 4827 5072 513 Size: 552 939 75 1761 987 245 3074 Fragments arranged by size: 3074 1761 987 939 552 245 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2142 5660 5677 5830 6015 6460 325 Size: 1255 562 3518 17 153 185 445 1498 Fragments arranged by size: 3518 1498 1255 562 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 2379 5879 Size: 274 80 33 51 1443 216 186 3500 Cuts at: 5879 7591 96 Size: 1712 138 Fragments arranged by size: 3500 1712 1443 274 216 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3770 3802 7593 7625 3770 Size: 32 3791 32 3778 Fragments arranged by size: 3791 3778 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3887 1316 Size: 2571 5062 XbaI T'CTAG_A Cuts at: 3493 4969 3493 Size: 1476 6157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2151 2798 3999 4169 4267 2151 Size: 647 1201 170 98 5517 Fragments arranged by size: 5517 1201 647 170 98 XhoI C'TCGA_G Cuts at: 1943 4769 1943 Size: 2826 4807 XmaI C'CCGG_G Cuts at: 1870 2065 2566 2966 3192 3852 4886 4958 1870 Size: 195 501 400 226 660 1034 72 4545 Fragments arranged by size: 4545 1034 660 501 400 226 195 72 XmnI GAAnn'nnTTC Cuts at: 5762 5762 Size: 7633 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5441 276 Size: 53 83 186 936 3907 2468 Fragments arranged by size: 3907 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SexAI SfiI SfoI SwaI