(Linear) (Six-base) MAPSORT of: KIEE3339.seq Check: 4627 from: 1 to: 2928 KIEE3339.orf With 182 enzymes: SgfI * July 3, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1212 2928 Size: 1212 1716 Acc65I G'GTAC_C Cuts at: 0 1475 2525 2928 Size: 1475 1050 403 Fragments arranged by size: 1475 1050 403 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1807 2405 2611 2928 Size: 1807 598 206 317 Fragments arranged by size: 1807 598 317 206 AfeI AGC'GCT Cuts at: 0 508 607 2928 Size: 508 99 2321 Fragments arranged by size: 2321 508 99 AflIII A'CryG_T Cuts at: 0 191 2928 Size: 191 2737 AgeI A'CCGG_T Cuts at: 0 2528 2928 Size: 2528 400 AhdI GACnn_n'nnGTC Cuts at: 0 477 2928 Size: 477 2451 AleI CACnn'nnGTG Cuts at: 0 1759 2928 Size: 1759 1169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 920 2666 2928 Size: 920 1746 262 Fragments arranged by size: 1746 920 262 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 283 315 2928 Size: 283 32 2613 Fragments arranged by size: 2613 283 32 AlwNI CAG_nnn'CTG Cuts at: 0 171 2383 2928 Size: 171 2212 545 Fragments arranged by size: 2212 545 171 ApaI G_GGCC'C Cuts at: 0 973 1145 1188 2928 Size: 973 172 43 1740 Fragments arranged by size: 1740 973 172 43 ApaLI G'TGCA_C Cuts at: 0 728 1168 2928 Size: 728 440 1760 Fragments arranged by size: 1760 728 440 ApoI r'AATT_y Cuts at: 0 2107 2928 Size: 2107 821 AvaI C'yCGr_G Cuts at: 0 60 561 961 1187 1847 2764 2881 2928 Size: 60 501 400 226 660 917 117 47 Fragments arranged by size: 917 660 501 400 226 117 60 47 BamHI G'GATC_C Cuts at: 0 132 2735 2928 Size: 132 2603 193 Fragments arranged by size: 2603 193 132 BanI G'GyrC_C Cuts at: 0 565 768 782 1475 2525 2885 2928 Size: 565 203 14 693 1050 360 43 Fragments arranged by size: 1050 693 565 360 203 43 14 BanII G_rGCy'C Cuts at: 0 960 973 1145 1188 1196 1719 2127 2573 Size: 960 13 172 43 8 523 408 446 Cuts at: 2573 2928 Size: 355 Fragments arranged by size: 960 523 446 408 355 172 43 13 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 588 2928 Size: 588 2340 BbvCI CC'TCA_GC Cuts at: 0 2849 2928 Size: 2849 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1804 1838 2928 Size: 1804 34 1090 Fragments arranged by size: 1804 1090 34 BclI T'GATC_A Cuts at: 0 1587 2096 2928 Size: 1587 509 832 Fragments arranged by size: 1587 832 509 BglI GCCn_nnn'nGGC Cuts at: 0 21 574 755 845 2723 2928 Size: 21 553 181 90 1878 205 Fragments arranged by size: 1878 553 205 181 90 21 BlpI GC'TnA_GC Cuts at: 0 2458 2928 Size: 2458 470 Bme1580I G_kGCm'C Cuts at: 0 732 892 973 1126 1145 1172 1188 2604 Size: 732 160 81 153 19 27 16 1416 Cuts at: 2604 2888 2928 Size: 284 40 Fragments arranged by size: 1416 732 284 160 153 81 40 27 19 16 BmrI ACTGGGnnnn_n' Cuts at: 0 1107 1573 2928 Size: 1107 466 1355 Fragments arranged by size: 1355 1107 466 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 528 2928 Size: 528 2400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1652 1789 2659 2857 2928 Size: 1652 137 870 198 71 Fragments arranged by size: 1652 870 198 137 71 Bpu10I CC'TnA_GC Cuts at: 0 1015 2849 2928 Size: 1015 1834 79 Fragments arranged by size: 1834 1015 79 BsaI GGTCTCn'nnnn_ Cuts at: 0 352 464 610 2928 Size: 352 112 146 2318 Fragments arranged by size: 2318 352 146 112 BsaAI yAC'GTr Cuts at: 0 288 887 1452 2928 Size: 288 599 565 1476 Fragments arranged by size: 1476 599 565 288 BsaBI GATnn'nnATC Cuts at: 0 1763 2095 2365 2869 2928 Size: 1763 332 270 504 59 Fragments arranged by size: 1763 504 332 270 59 BsaHI Gr'CG_yC Cuts at: 0 1080 2069 2928 Size: 1080 989 859 Fragments arranged by size: 1080 989 859 BsaWI w'CCGG_w Cuts at: 0 854 1703 2528 2928 Size: 854 849 825 400 Fragments arranged by size: 854 849 825 400 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 283 313 1628 1658 2647 2677 2928 Size: 283 30 1315 30 989 30 251 Fragments arranged by size: 1315 989 283 251 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 1649 2928 Size: 283 1366 1279 Fragments arranged by size: 1366 1279 283 BseYI C'CCAG_C Cuts at: 0 117 664 893 1062 1281 1718 1936 2585 Size: 117 547 229 169 219 437 218 649 Cuts at: 2585 2713 2809 2928 Size: 128 96 119 Fragments arranged by size: 649 547 437 229 219 218 169 128 119 117 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1432 1694 1808 2779 2928 Size: 1432 262 114 971 149 Fragments arranged by size: 1432 971 262 149 114 BsiHKAI G_wGCw'C Cuts at: 0 732 904 960 1172 2573 2928 Size: 732 172 56 212 1401 355 Fragments arranged by size: 1401 732 355 212 172 56 BsmI GAATG_Cn' Cuts at: 0 401 1556 2897 2928 Size: 401 1155 1341 31 Fragments arranged by size: 1341 1155 401 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2757 2928 Size: 2757 171 Bsp1286I G_dGCh'C Cuts at: 0 732 892 904 960 973 1126 1145 1172 Size: 732 160 12 56 13 153 19 27 Cuts at: 1172 1188 1196 1719 2127 2573 2604 2888 2928 Size: 16 8 523 408 446 31 284 40 Fragments arranged by size: 732 523 446 408 284 160 153 56 40 31 27 19 16 13 12 8 BspHI T'CATG_A Cuts at: 0 1590 2093 2573 2928 Size: 1590 503 480 355 Fragments arranged by size: 1590 503 480 355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 697 1212 2928 Size: 697 515 1716 Fragments arranged by size: 1716 697 515 BsrBI CCG'CTC Cuts at: 0 6 944 2148 2928 Size: 6 938 1204 780 Fragments arranged by size: 1204 938 780 6 BsrDI GCAATG_nn' Cuts at: 0 2390 2675 2928 Size: 2390 285 253 Fragments arranged by size: 2390 285 253 BsrFI r'CCGG_y Cuts at: 0 2528 2606 2928 Size: 2528 78 322 Fragments arranged by size: 2528 322 78 BsrGI T'GTAC_A Cuts at: 0 188 374 2928 Size: 188 186 2554 Fragments arranged by size: 2554 188 186 BssHII G'CGCG_C Cuts at: 0 67 69 2928 Size: 67 2 2859 Fragments arranged by size: 2859 67 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 743 832 2055 2903 2928 Size: 743 89 1223 848 25 Fragments arranged by size: 1223 848 743 89 25 BstEII G'GTnAC_C Cuts at: 0 432 2928 Size: 432 2496 BstXI CCAn_nnnn'nTGG Cuts at: 0 1156 1761 2809 2928 Size: 1156 605 1048 119 Fragments arranged by size: 1156 1048 605 119 BstYI r'GATC_y Cuts at: 0 132 254 2360 2402 2735 2928 Size: 132 122 2106 42 333 193 Fragments arranged by size: 2106 333 193 132 122 42 Bsu36I CC'TnA_GG Cuts at: 0 723 1029 1927 2140 2928 Size: 723 306 898 213 788 Fragments arranged by size: 898 788 723 306 213 BtgI C'CryG_G Cuts at: 0 261 437 534 2822 2928 Size: 261 176 97 2288 106 Fragments arranged by size: 2288 261 176 106 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2435 2928 Size: 2435 493 BtsI GCAGTG_nn' Cuts at: 0 741 822 902 1214 2875 2928 Size: 741 81 80 312 1661 53 Fragments arranged by size: 1661 741 312 81 80 53 DrdI GACnn_nn'nnGTC Cuts at: 0 2161 2928 Size: 2161 767 EaeI y'GGCC_r Cuts at: 0 945 1293 1958 1992 2180 2608 2928 Size: 945 348 665 34 188 428 320 Fragments arranged by size: 945 665 428 348 320 188 34 EarI CTCTTCn'nnn_ Cuts at: 0 2503 2708 2928 Size: 2503 205 220 Fragments arranged by size: 2503 220 205 EcoICRI GAG'CTC Cuts at: 0 958 2571 2928 Size: 958 1613 357 Fragments arranged by size: 1613 958 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1652 1789 1807 2405 2611 2659 2857 2928 Size: 1652 137 18 598 206 48 198 71 Fragments arranged by size: 1652 598 206 198 137 71 48 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 2014 2139 2928 Size: 2014 125 789 Fragments arranged by size: 2014 789 125 EcoO109I rG'GnC_Cy Cuts at: 0 686 719 969 970 991 1141 1142 1184 Size: 686 33 250 1 21 150 1 42 Cuts at: 1184 1406 1567 1623 1818 1922 2244 2853 2928 Size: 222 161 56 195 104 322 609 75 Fragments arranged by size: 686 609 322 250 222 195 161 150 104 75 56 42 33 21 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 378 2928 Size: 378 2550 HaeII r_GCGC'y Cuts at: 0 12 510 609 2928 Size: 12 498 99 2319 Fragments arranged by size: 2319 498 99 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 1327 1359 2345 2377 2478 2510 Size: 463 32 832 32 986 32 101 32 Cuts at: 2510 2727 2759 2928 Size: 217 32 169 Fragments arranged by size: 986 832 463 217 169 101 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 2623 2928 Size: 2623 305 KpnI G_GTAC'C Cuts at: 0 1479 2529 2928 Size: 1479 1050 399 Fragments arranged by size: 1479 1050 399 MfeI C'AATT_G Cuts at: 0 249 2928 Size: 249 2679 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 435 2353 2928 Size: 435 1918 575 Fragments arranged by size: 1918 575 435 MscI TGG'CCA Cuts at: 0 1295 1960 1994 2182 2928 Size: 1295 665 34 188 746 Fragments arranged by size: 1295 746 665 188 34 MslI CAynn'nnrTG Cuts at: 0 1217 1321 1759 2231 2266 2776 2928 Size: 1217 104 438 472 35 510 152 Fragments arranged by size: 1217 510 472 438 152 104 35 MspA1I CmG'CkG Cuts at: 0 54 263 664 1722 2160 2848 2928 Size: 54 209 401 1058 438 688 80 Fragments arranged by size: 1058 688 438 401 209 80 54 NaeI GCC'GGC Cuts at: 0 2608 2928 Size: 2608 320 NcoI C'CATG_G Cuts at: 0 2822 2928 Size: 2822 106 NdeI CA'TA_TG Cuts at: 0 2592 2928 Size: 2592 336 NgoMIV G'CCGG_C Cuts at: 0 2606 2928 Size: 2606 322 NspI r_CATG'y Cuts at: 0 195 741 830 986 1216 1333 2928 Size: 195 546 89 156 230 117 1595 Fragments arranged by size: 1595 546 230 195 156 117 89 PasI CC'CwG_GG Cuts at: 0 18 328 1423 1496 2167 2349 2693 2928 Size: 18 310 1095 73 671 182 344 235 Fragments arranged by size: 1095 671 344 310 235 182 73 18 PciI A'CATG_T Cuts at: 0 191 2928 Size: 191 2737 PflMI CCAn_nnn'nTGG Cuts at: 0 145 396 2928 Size: 145 251 2532 Fragments arranged by size: 2532 251 145 PfoI T'CCnGG_A Cuts at: 0 1708 2318 2928 Size: 1708 610 610 Fragments arranged by size: 1708 610 610 PmlI CAC'GTG Cuts at: 0 288 887 1452 2928 Size: 288 599 565 1476 Fragments arranged by size: 1476 599 565 288 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 283 315 2928 Size: 283 32 2613 Fragments arranged by size: 2613 283 32 PpuMI rG'GwC_Cy Cuts at: 0 686 991 1406 1567 1818 1922 2244 2853 Size: 686 305 415 161 251 104 322 609 Cuts at: 2853 2928 Size: 75 Fragments arranged by size: 686 609 415 322 305 251 161 104 75 PspOMI G'GGCC_C Cuts at: 0 969 1141 1184 2928 Size: 969 172 43 1744 Fragments arranged by size: 1744 969 172 43 PspXI vC'TCGA_Gb Cuts at: 0 2764 2928 Size: 2764 164 PstI C_TGCA'G Cuts at: 0 299 450 748 1209 1520 2192 2928 Size: 299 151 298 461 311 672 736 Fragments arranged by size: 736 672 461 311 299 298 151 PvuII CAG'CTG Cuts at: 0 2160 2928 Size: 2160 768 SacI G_AGCT'C Cuts at: 0 960 2573 2928 Size: 960 1613 355 Fragments arranged by size: 1613 960 355 SacII CC_GC'GG Cuts at: 0 264 2928 Size: 264 2664 SanDI GG'GwC_CC Cuts at: 0 1406 1922 2928 Size: 1406 516 1006 Fragments arranged by size: 1406 1006 516 SapI GCTCTTCn'nnn_ Cuts at: 0 2503 2928 Size: 2503 425 SfcI C'TryA_G Cuts at: 0 295 446 744 773 1205 1516 2188 2928 Size: 295 151 298 29 432 311 672 740 Fragments arranged by size: 740 672 432 311 298 295 151 29 SmaI CCC'GGG Cuts at: 0 62 563 963 1189 1849 2883 2928 Size: 62 501 400 226 660 1034 45 Fragments arranged by size: 1034 660 501 400 226 62 45 SmlI C'TyrA_G Cuts at: 0 2764 2928 Size: 2764 164 SphI G_CATG'C Cuts at: 0 741 986 1333 2928 Size: 741 245 347 1595 Fragments arranged by size: 1595 741 347 245 SrfI GCCC'GGGC Cuts at: 0 2883 2928 Size: 2883 45 StuI AGG'CCT Cuts at: 0 1349 2928 Size: 1349 1579 StyI C'CwwG_G Cuts at: 0 74 1835 2822 2928 Size: 74 1761 987 106 Fragments arranged by size: 1761 987 106 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 137 2928 Size: 137 2791 TatI w'GTAC_w Cuts at: 0 188 374 2928 Size: 188 186 2554 Fragments arranged by size: 2554 188 186 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1765 1797 2928 Size: 1765 32 1131 Fragments arranged by size: 1765 1131 32 Tth111I GACn'n_nGTC Cuts at: 0 1882 2928 Size: 1882 1046 XbaI T'CTAG_A Cuts at: 0 1488 2928 Size: 1488 1440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 146 793 1994 2164 2262 2928 Size: 146 647 1201 170 98 666 Fragments arranged by size: 1201 666 647 170 146 98 XhoI C'TCGA_G Cuts at: 0 2764 2928 Size: 2764 164 XmaI C'CCGG_G Cuts at: 0 60 561 961 1187 1847 2881 2928 Size: 60 501 400 226 660 1034 47 Fragments arranged by size: 1034 660 501 400 226 60 47 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SfcI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AflII AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BglII BmgBI BmtI BpuEI BsiEI BsiWI BspEI BssSI BstBI BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI MluI NarI NheI NotI NruI NsiI PacI PmeI PshAI PsiI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI XmnI ZraI