Restriction map for
pFN21AE4119
(Circular) (Six-base) MAPSORT of: pFN21AE4119.seq Check: 983 from: 1 to: 6127
pFN21AE4119 6127 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3336 3336
Size: 6127
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3129 3937 278
Size: 53 83 186 936 1593 808 2468
Fragments arranged by size:
2468 1593 936 808 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 3045 4254 4627 1563
Size: 1482 1209 373 3063
Fragments arranged by size:
3063 1482 1209 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2705 2883 2937 4190 5574 5842 1632
Size: 932 141 178 54 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 932 268 178 141 54
AfeI AGC'GCT
Cuts at: 3310 3310
Size: 6127
AflII C'TTAA_G
Cuts at: 820 1017 2408 820
Size: 197 1391 4539
Fragments arranged by size:
4539 1391 197
AflIII A'CryG_T
Cuts at: 1203 2631 5026 6003 1203
Size: 1428 2395 977 1327
Fragments arranged by size:
2395 1428 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 2071 4856 2071
Size: 2785 3342
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 3416 3870 5442 3416
Size: 454 1572 4101
Fragments arranged by size:
4101 1572 454
ApaI G_GGCC'C
Cuts at: 2203 2246 2203
Size: 43 6084
ApaLI G'TGCA_C
Cuts at: 3323 4184 5340 3323
Size: 861 1156 4110
Fragments arranged by size:
4110 1156 861
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 3452 1870
Size: 73 311 1198 4545
Fragments arranged by size:
4545 1198 311 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2163 2173 2235 2365
Size: 325 217 627 338 38 10 62 130
Cuts at: 2365 3448 4903 618
Size: 1083 1455 1842
Fragments arranged by size:
1842 1455 1083 627 338 325 217 130
62 38 10
BanII G_rGCy'C
Cuts at: 1391 2203 2246 3446 1391
Size: 812 43 1200 4072
Fragments arranged by size:
4072 1200 812 43
BbeI G_GCGC'C
Cuts at: 2129 2177 2239 2129
Size: 48 62 6017
Fragments arranged by size:
6017 62 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2228 2801 2871 2881 2999 928
Size: 971 329 573 70 10 118 4056
Fragments arranged by size:
4056 971 573 329 118 70 10
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2452 2452
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2265 2287 4738
Size: 107 122 71 631 605 593 22 2451
Cuts at: 4738 136
Size: 1525
Fragments arranged by size:
2451 1525 631 605 593 122 107 71
22
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2203 2246 2253 3327 4188 5344 1790
Size: 413 43 7 1074 861 1156 2573
Fragments arranged by size:
2573 1156 1074 861 413 43 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2131 2206 2284
Size: 416 53 83 399 24 714 75 78
Cuts at: 2284 3079 3297 3858 4816 442
Size: 795 218 561 958 1753
Fragments arranged by size:
1753 958 795 714 561 416 399 218
83 78 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2718 3003 2718
Size: 285 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 3116 4787 1239
Size: 1493 384 1671 2579
Fragments arranged by size:
2579 1671 1493 384
Bpu10I CC'TnA_GC
Cuts at: 2013 2013
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2911 3918 493
Size: 711 1707 1007 2702
Fragments arranged by size:
2702 1707 1007 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 2174 2236 3126
Size: 53 83 186 936 593 48 62 890
Cuts at: 3126 3934 4316 4951 275
Size: 808 382 635 1451
Fragments arranged by size:
1451 936 890 808 635 593 382 186
83 62 53 48
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 1186
Size: 856 5271
BseYI C'CCAG_C
Cuts at: 3237 5330 5826 3237
Size: 2093 496 3538
Fragments arranged by size:
3538 2093 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2350 2528 3314 1163
Size: 573 360 254 178 786 3976
Fragments arranged by size:
3976 786 573 360 254 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2269 3577 4338 5366 1540
Size: 275 186 268 1308 761 1028 2301
Fragments arranged by size:
2301 1308 1028 761 275 268 186
BsiHKAI G_wGCw'C
Cuts at: 2349 3327 4188 4273 5344 2349
Size: 978 861 85 1071 3132
Fragments arranged by size:
3132 1071 978 861 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2600
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 2246 2253 2349 3327 3446 4188
Size: 399 413 43 7 96 978 119 742
Cuts at: 4188 4273 5344 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 978 742 413 399 119 96
85 43 7
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 3336 3482 844
Size: 1201 1291 146 3489
Fragments arranged by size:
3489 1291 1201 146
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2232 4771 4958 1700
Size: 110 422 2539 187 2869
Fragments arranged by size:
2869 2539 422 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 2426 4187 5199 2426
Size: 1761 1012 3354
Fragments arranged by size:
3354 1761 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 3375 2050
Size: 1325 4802
BstYI r'GATC_y
Cuts at: 1972 2754 3457 3824 4209 4226 5678 5764 5776
Size: 782 703 367 385 17 1452 86 12
Cuts at: 5776 6122 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 782 703 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3055 3055
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 2361 513
Size: 552 939 97 260 4279
Fragments arranged by size:
4279 939 552 260 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DraIII CAC_nnn'GTG
Cuts at: 2487 3262 2487
Size: 775 5352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2205 2266
Size: 54 1425 50 275 18 256 119 61
Cuts at: 2266 2312 2430 2939 3365 3574 4463 6092 8
Size: 46 118 509 426 209 889 1629 43
Fragments arranged by size:
1629 1425 889 509 426 275 256 209
119 118 61 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2266 3574 1537
Size: 275 454 1308 4090
Fragments arranged by size:
4090 1308 454 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 2559 2598 2687 4057 1353
Size: 944 262 39 89 1370 3423
Fragments arranged by size:
3423 1370 944 262 89 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2705 2732 2883 2937 3116 4190
Size: 393 932 141 27 151 54 179 1074
Cuts at: 4190 4787 5574 5842 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1074 932 787 597 393 268 179
151 141 54 27
EcoO109I rG'GnC_Cy
Cuts at: 2065 2200 2243 2569 2065
Size: 135 43 326 5623
Fragments arranged by size:
5623 326 135 43
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2129 2177 2233 2239 3312 5274 1678
Size: 451 48 56 6 1073 1962 2531
Fragments arranged by size:
2531 1962 1073 451 56 48 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2491 2523 4768
Size: 32 135 32 485 32 402 32 2245
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2245 485 402 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2034 2742 3471 3717 669
Size: 988 281 96 708 729 246 3079
Fragments arranged by size:
3079 988 729 708 281 246 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KasI G'GCGC_C
Cuts at: 2125 2173 2235 2125
Size: 48 62 6017
Fragments arranged by size:
6017 62 48
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2207 2432 2941 10
Size: 54 1425 343 375 225 509 3196
Fragments arranged by size:
3196 1425 509 375 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4085 4444 4603 518
Size: 648 2919 359 159 2042
Fragments arranged by size:
2919 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2284 2311 3543 4220 5368 5613 1606
Size: 497 181 27 1232 677 1148 245 2120
Fragments arranged by size:
2120 1232 1148 677 497 245 181 27
NaeI GCC'GGC
Cuts at: 2234 2234
Size: 6127
NarI GG'CG_CC
Cuts at: 2126 2174 2236 2126
Size: 48 62 6017
Fragments arranged by size:
6017 62 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 2361 513
Size: 552 939 357 4279
Fragments arranged by size:
4279 939 552 357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NgoMIV G'CCGG_C
Cuts at: 2232 2232
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 2266 3574 2266
Size: 1308 4819
NsiI A_TGCA'T
Cuts at: 2454 2454
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 3485 5030 1132
Size: 97 2256 1545 2229
Fragments arranged by size:
2256 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2487 2937 3262 1108
Size: 1379 450 325 3973
Fragments arranged by size:
3973 1379 450 325
PfoI T'CCnGG_A
Cuts at: 2022 2061 2757 2022
Size: 39 696 5392
Fragments arranged by size:
5392 696 39
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2569 2065
Size: 504 5623
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 2199 2242 2199
Size: 43 6084
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
SacII CC_GC'GG
Cuts at: 2104 2104
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SapI GCTCTTCn'nnn_
Cuts at: 2297 2687 2297
Size: 390 5737
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 3304 3475 4610 5291 5482 826
Size: 219 2259 171 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 2265 2265
Size: 6127
SfoI GGC'GCC
Cuts at: 2127 2175 2237 2127
Size: 48 62 6017
Fragments arranged by size:
6017 62 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 2232 1700
Size: 532 5595
SmaI CCC'GGG
Cuts at: 1872 2256 3454 1872
Size: 384 1198 4545
Fragments arranged by size:
4545 1198 384
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 4231 5132 5394 820
Size: 197 926 465 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 465 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 1517
Size: 864 5263
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 3566 513
Size: 552 939 357 1205 3074
Fragments arranged by size:
3074 1205 939 552 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2181 2383 3251 4154 4171 4324 4509
Size: 1255 601 202 868 903 17 153 185
Cuts at: 4509 4954 325
Size: 445 1498
Fragments arranged by size:
1498 1255 903 868 601 445 202 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4373 6085 96
Size: 274 80 33 51 1443 2396 1712 138
Fragments arranged by size:
2396 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 6087 6119 2860
Size: 32 3195 32 2868
Fragments arranged by size:
3195 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2200
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 2254 3452 1870
Size: 384 1198 4545
Fragments arranged by size:
4545 1198 384
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3127 3935 276
Size: 53 83 186 936 1593 808 2468
Fragments arranged by size:
2468 1593 936 808 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I EcoICRI EcoNI
EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SrfI SwaI