Restriction map for
pFN21AE0973
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KpnI MamI MfeI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI SbfI ScaI SexAI
SfaNI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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ApaI 1305 GGGCCC GGGCC^C
BanI 700 GGCACC G^G[CT][GA]CC
BfaI 649 CTAG C^TAG
BseRI 467 GAGGAG GAGGAG(10/8)
BsgI 345 GTGCAG GTGCAG(16/14)
BsiEI 86 CGGCCG CG[GA][CT]^CG
BsiWI 185 CGTACG C^GTACG
BspHI 410 TCATGA T^CATGA
BspLU11I 764 ACATGT A^CATGT
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BsrFI 102 GCCGGC [GA]^CCGG[CT]
BstAPI 1246 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1114 GGTCACC G^GT[ACGT]ACC
BstPI 1114 GGTCACC G^GT[ACGT]ACC
BstYI 960 AGATCC [GA]^GATC[CT]
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
DraII 1304 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 119 CCGCGG C^C[GA][CT]GG
EaeI 86 CGGCCG [CT]^GGCC[GA]
EagI 86 CGGCCG C^GGCCG
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
EcoO109I 1304 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1114 GGTCACC G^GT[ACGT]ACC
EcoRV 442 GATATC GAT^ATC
FauI 118 CCCGC CCCGC(4/6)
HgaI 181 GACGC GACGC(5/10)
KspI 119 CCGCGG CCGC^GG
MaeI 649 CTAG C^TAG
MflI 960 AGATCC [GA]^GATC[CT]
MluI 182 ACGCGT A^CGCGT
NaeI 102 GCCGGC GCC^GGC
NgoMIV 102 GCCGGC G^CCGGC
NotI 85 GCGGCCGC GC^GGCCGC
NspI 764 ACATGT [GA]CATG^[CT]
RcaI 410 TCATGA T^CATGA
SacI 1453 GAGCTC GAGCT^C
SacII 119 CCGCGG CCGC^GG
SfiI 78 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 589 AATATT AAT^ATT
XhoII 960 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 326 AAATTC [GA]^AATT[CT]
1003 AAATTC
Alw44I 25 GTGCAC G^TGCAC
1291 GTGCAC
ApaLI 25 GTGCAC G^TGCAC
1291 GTGCAC
ApoI 326 AAATTC [GA]^AATT[CT]
1003 AAATTC
BanII 1305 GGGCCC G[GA]GC[CT]^C
1453 GAGCTC
BglI 79 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
142 GCCCCGGCGGC
BpmI 1380 CTCCAG CTGGAG(16/14)
1418 CTCCAG
BsrI 1142 ACTGG ACTGG(1/-1)
302 CCAGT
CfoI 100 GCGC GCG^C
811 GCGC
DraI 993 TTTAAA TTT^AAA
1539 TTTAAA
HaeII 99 AGCGCC [GA]GCGC^[CT]
810 AGCGCC
HhaI 100 GCGC GCG^C
811 GCGC
HinP1I 100 GCGC G^CGC
811 GCGC
HincII 460 GTCAAC GT[CT]^[GA]AC
849 GTTGAC
HindII 460 GTCAAC GT[CT]^[GA]AC
849 GTTGAC
HindIII 1337 AAGCTT A^AGCTT
1535 AAGCTT
MslI 475 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CATGAAAGTG
MspA1I 62 CAGCGG C[AC]G^C[GT]G
119 CCGCGG
PleI 1297 GACTC GAGTC(4/5)
339 GAGTC
PstI 174 CTGCAG CTGCA^G
193 CTGCAG
SapI 1173 GAAGAGC GCTCTTC(1/4)
1455 GCTCTTC
TfiI 564 GATTC G^A[AT]TC
1587 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI